Neisseria sp. oral taxon 020 str. F0370
Average proteome isoelectric point is 7.12
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2879 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|L1P2Q6|L1P2Q6_9NEIS NAD dependent epimerase/dehydratase family protein (Fragment) OS=Neisseria sp. oral taxon 020 str. F0370 OX=1127694 GN=HMPREF9120_00172 PE=4 SV=1
MM1 pKa = 7.4 FPHH4 pKa = 7.41 PDD6 pKa = 3.27 DD7 pKa = 4.02 EE8 pKa = 4.66 EE9 pKa = 4.63 YY10 pKa = 11.43 NRR12 pKa = 11.84 FIRR15 pKa = 11.84 AALGSRR21 pKa = 11.84 TLYY24 pKa = 10.39 TLVSPEE30 pKa = 4.64 DD31 pKa = 4.11 DD32 pKa = 3.62 VAEE35 pKa = 4.61 CPSAEE40 pKa = 4.01 YY41 pKa = 10.77 EE42 pKa = 4.16 EE43 pKa = 6.1 DD44 pKa = 3.5 DD45 pKa = 4.47 GEE47 pKa = 4.32 PVPVFCVWHH56 pKa = 6.96 DD57 pKa = 3.49 RR58 pKa = 11.84 AQAEE62 pKa = 4.13 ACIAEE67 pKa = 4.38 EE68 pKa = 4.22 WADD71 pKa = 3.74 HH72 pKa = 6.31 EE73 pKa = 4.86 VDD75 pKa = 4.23 EE76 pKa = 5.71 LPLDD80 pKa = 4.3 FFLHH84 pKa = 5.71 EE85 pKa = 4.55 WLVGMDD91 pKa = 3.42 QDD93 pKa = 5.13 AVLLGIDD100 pKa = 5.39 FDD102 pKa = 4.12 PQLYY106 pKa = 10.15 GLEE109 pKa = 4.05 IEE111 pKa = 4.42 PVEE114 pKa = 4.2 VLADD118 pKa = 3.26 ILDD121 pKa = 4.09 AAEE124 pKa = 4.3 QMQCTEE130 pKa = 4.49 MIDD133 pKa = 4.12 GYY135 pKa = 10.99 GEE137 pKa = 4.03 LMNYY141 pKa = 8.95 RR142 pKa = 11.84 LEE144 pKa = 4.37 WEE146 pKa = 4.35 RR147 pKa = 11.84 EE148 pKa = 3.58 MAGQKK153 pKa = 10.11 RR154 pKa = 11.84 LNN156 pKa = 3.5
Molecular weight: 18.0 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.718
IPC2_protein 3.808
IPC_protein 3.783
Toseland 3.592
ProMoST 3.923
Dawson 3.757
Bjellqvist 3.91
Wikipedia 3.656
Rodwell 3.617
Grimsley 3.503
Solomon 3.745
Lehninger 3.706
Nozaki 3.872
DTASelect 4.037
Thurlkill 3.63
EMBOSS 3.668
Sillero 3.897
Patrickios 1.1
IPC_peptide 3.745
IPC2_peptide 3.884
IPC2.peptide.svr19 3.808
Protein with the highest isoelectric point:
>tr|L1NSF4|L1NSF4_9NEIS Protein CreA OS=Neisseria sp. oral taxon 020 str. F0370 OX=1127694 GN=HMPREF9120_01556 PE=4 SV=1
MM1 pKa = 7.62 LPRR4 pKa = 11.84 RR5 pKa = 11.84 SKK7 pKa = 10.69 PPRR10 pKa = 11.84 KK11 pKa = 8.82 QQGRR15 pKa = 11.84 LKK17 pKa = 9.6 TPPRR21 pKa = 11.84 QGRR24 pKa = 11.84 HH25 pKa = 4.26 FQTAFSLLPAPLVARR40 pKa = 11.84 TMRR43 pKa = 11.84 QMII46 pKa = 4.07
Molecular weight: 5.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.493
IPC2_protein 11.096
IPC_protein 12.691
Toseland 12.852
ProMoST 13.349
Dawson 12.852
Bjellqvist 12.852
Wikipedia 13.334
Rodwell 12.501
Grimsley 12.896
Solomon 13.349
Lehninger 13.247
Nozaki 12.852
DTASelect 12.852
Thurlkill 12.852
EMBOSS 13.349
Sillero 12.852
Patrickios 12.237
IPC_peptide 13.349
IPC2_peptide 12.34
IPC2.peptide.svr19 9.129
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2879
0
2879
704475
39
2408
244.7
26.85
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.012 ± 0.081
1.11 ± 0.016
5.26 ± 0.042
5.779 ± 0.053
4.202 ± 0.039
7.975 ± 0.062
2.164 ± 0.025
4.94 ± 0.045
4.775 ± 0.049
10.011 ± 0.074
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.346 ± 0.029
3.551 ± 0.041
4.61 ± 0.039
4.132 ± 0.04
6.3 ± 0.047
5.246 ± 0.038
4.968 ± 0.037
6.583 ± 0.048
1.25 ± 0.021
2.785 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here