Corynebacterium kutscheri
Average proteome isoelectric point is 6.08
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2047 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0F6R2H8|A0A0F6R2H8_9CORY Transcriptional regulator OS=Corynebacterium kutscheri OX=35755 GN=UL82_07535 PE=4 SV=1
MM1 pKa = 7.56 SLPEE5 pKa = 3.97 NFSYY9 pKa = 10.78 SEE11 pKa = 3.7 EE12 pKa = 4.34 HH13 pKa = 5.73 EE14 pKa = 4.8 WINSTADD21 pKa = 3.3 AAVGTTVKK29 pKa = 10.89 VGITSIAADD38 pKa = 3.64 RR39 pKa = 11.84 LGEE42 pKa = 4.26 VVFAEE47 pKa = 4.81 LPQVGDD53 pKa = 3.82 SIEE56 pKa = 4.35 AGEE59 pKa = 4.36 TCGEE63 pKa = 4.19 VEE65 pKa = 4.36 STKK68 pKa = 10.75 SVSDD72 pKa = 3.74 LYY74 pKa = 11.51 APVTGTVIAVNDD86 pKa = 4.2 AVHH89 pKa = 7.42 DD90 pKa = 4.55 DD91 pKa = 3.67 YY92 pKa = 11.9 AIINNDD98 pKa = 3.16 PFGQGWLFEE107 pKa = 4.47 VEE109 pKa = 4.17 VEE111 pKa = 4.96 SIGEE115 pKa = 4.06 LLTAEE120 pKa = 4.89 EE121 pKa = 4.33 YY122 pKa = 11.19 GKK124 pKa = 11.14 ANGVV128 pKa = 3.29
Molecular weight: 13.57 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.78
IPC2_protein 3.808
IPC_protein 3.732
Toseland 3.554
ProMoST 3.872
Dawson 3.694
Bjellqvist 3.846
Wikipedia 3.579
Rodwell 3.567
Grimsley 3.478
Solomon 3.668
Lehninger 3.63
Nozaki 3.821
DTASelect 3.935
Thurlkill 3.592
EMBOSS 3.605
Sillero 3.846
Patrickios 1.825
IPC_peptide 3.681
IPC2_peptide 3.821
IPC2.peptide.svr19 3.754
Protein with the highest isoelectric point:
>tr|A0A0F6TC53|A0A0F6TC53_9CORY Dihydrolipoyl dehydrogenase OS=Corynebacterium kutscheri OX=35755 GN=lpd PE=3 SV=1
MM1 pKa = 7.69 AKK3 pKa = 10.06 GKK5 pKa = 8.69 RR6 pKa = 11.84 TFQPNNRR13 pKa = 11.84 RR14 pKa = 11.84 RR15 pKa = 11.84 AKK17 pKa = 10.01 KK18 pKa = 8.51 HH19 pKa = 4.28 GFRR22 pKa = 11.84 IRR24 pKa = 11.84 MRR26 pKa = 11.84 TRR28 pKa = 11.84 AGRR31 pKa = 11.84 AIVAARR37 pKa = 11.84 RR38 pKa = 11.84 SKK40 pKa = 10.76 GRR42 pKa = 11.84 AKK44 pKa = 9.67 LTAA47 pKa = 4.21
Molecular weight: 5.43 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.525
IPC2_protein 11.286
IPC_protein 12.881
Toseland 13.042
ProMoST 13.539
Dawson 13.056
Bjellqvist 13.042
Wikipedia 13.525
Rodwell 12.735
Grimsley 13.086
Solomon 13.539
Lehninger 13.451
Nozaki 13.042
DTASelect 13.042
Thurlkill 13.042
EMBOSS 13.554
Sillero 13.042
Patrickios 12.457
IPC_peptide 13.554
IPC2_peptide 12.53
IPC2.peptide.svr19 9.203
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2047
0
2047
665480
17
2988
325.1
35.47
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.589 ± 0.059
0.787 ± 0.015
5.545 ± 0.051
6.026 ± 0.052
3.555 ± 0.035
7.623 ± 0.048
2.3 ± 0.028
6.43 ± 0.042
3.763 ± 0.051
9.725 ± 0.061
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.185 ± 0.025
3.394 ± 0.035
4.631 ± 0.037
3.725 ± 0.032
5.486 ± 0.052
6.257 ± 0.041
6.5 ± 0.057
7.69 ± 0.049
1.311 ± 0.027
2.479 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here