Fluviicola sp. SGL-29

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Crocinitomicaceae; Fluviicola; unclassified Fluviicola

Average proteome isoelectric point is 6.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3167 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6B5PHL2|A0A6B5PHL2_9FLAO GTPase Der OS=Fluviicola sp. SGL-29 OX=2710880 GN=der PE=3 SV=1
MM1 pKa = 7.25KK2 pKa = 10.03KK3 pKa = 10.5VYY5 pKa = 10.15FFTVALAATTTVFSQDD21 pKa = 3.11GADD24 pKa = 3.19TLTFEE29 pKa = 4.91SYY31 pKa = 11.49NLGTQDD37 pKa = 3.93YY38 pKa = 10.25YY39 pKa = 11.67NGSDD43 pKa = 3.33EE44 pKa = 5.48AGDD47 pKa = 3.53IVIGNFTLSNSYY59 pKa = 8.23NTEE62 pKa = 3.59WGSWSGFAVSKK73 pKa = 10.47VQDD76 pKa = 3.39VTTAGWGNQYY86 pKa = 11.48ASFANGGADD95 pKa = 3.24GSSQYY100 pKa = 11.2GMWYY104 pKa = 10.18NGGTIEE110 pKa = 4.38FNAAKK115 pKa = 9.81VLKK118 pKa = 10.3SIQITNATYY127 pKa = 8.98TAISMRR133 pKa = 11.84DD134 pKa = 2.86GDD136 pKa = 3.87QFAKK140 pKa = 10.61QFGSVNSAAGTPDD153 pKa = 3.25GTNGADD159 pKa = 2.96WFLLQIVPLNEE170 pKa = 3.54NDD172 pKa = 3.74EE173 pKa = 4.48LVGDD177 pKa = 4.5TIDD180 pKa = 5.71FYY182 pKa = 11.61LADD185 pKa = 4.02YY186 pKa = 10.92RR187 pKa = 11.84FADD190 pKa = 4.5DD191 pKa = 3.64NDD193 pKa = 4.16DD194 pKa = 4.32YY195 pKa = 11.55IVNSWEE201 pKa = 4.17TVDD204 pKa = 5.15LGNVTANKK212 pKa = 9.95LAFVLSSSDD221 pKa = 3.34NGEE224 pKa = 3.7WGMNTPGYY232 pKa = 10.35FSVDD236 pKa = 2.84NLVANNSLGIEE247 pKa = 4.23TLTQLNAVVYY257 pKa = 7.25PNPAQEE263 pKa = 4.35QFTIATTEE271 pKa = 3.95AADD274 pKa = 3.79MALLNAMGQVVRR286 pKa = 11.84TAVVNGTTVINVNGLPAGMYY306 pKa = 10.05QIALKK311 pKa = 8.49TASGSSVQKK320 pKa = 10.75LVIQQ324 pKa = 4.2

Molecular weight: 34.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6B5PMM3|A0A6B5PMM3_9FLAO Arginine--tRNA ligase OS=Fluviicola sp. SGL-29 OX=2710880 GN=argS PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVRR10 pKa = 11.84KK11 pKa = 9.55RR12 pKa = 11.84RR13 pKa = 11.84NKK15 pKa = 9.75HH16 pKa = 4.03GFRR19 pKa = 11.84SRR21 pKa = 11.84MATKK25 pKa = 10.11NGRR28 pKa = 11.84RR29 pKa = 11.84VLAARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.27GRR39 pKa = 11.84KK40 pKa = 8.73KK41 pKa = 9.94LTVSDD46 pKa = 3.97EE47 pKa = 4.27PMHH50 pKa = 6.8KK51 pKa = 10.24RR52 pKa = 3.38

Molecular weight: 6.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3167

0

3167

1122623

25

3087

354.5

39.79

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.61 ± 0.04

0.971 ± 0.018

5.187 ± 0.032

6.436 ± 0.057

5.011 ± 0.037

6.909 ± 0.053

1.952 ± 0.023

7.327 ± 0.037

6.507 ± 0.066

8.986 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.233 ± 0.02

5.65 ± 0.039

3.683 ± 0.029

3.779 ± 0.027

3.734 ± 0.032

6.552 ± 0.038

6.469 ± 0.075

6.681 ± 0.031

1.124 ± 0.015

4.198 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski