Amino acid dipepetide frequency for Fluviicola sp. SGL-29

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.79AlaAla: 4.79 ± 0.094
0.774AlaCys: 0.774 ± 0.028
3.574AlaAsp: 3.574 ± 0.062
4.075AlaGlu: 4.075 ± 0.078
3.249AlaPhe: 3.249 ± 0.06
5.053AlaGly: 5.053 ± 0.091
1.176AlaHis: 1.176 ± 0.034
5.072AlaIle: 5.072 ± 0.079
4.064AlaLys: 4.064 ± 0.085
5.839AlaLeu: 5.839 ± 0.085
1.48AlaMet: 1.48 ± 0.042
3.294AlaAsn: 3.294 ± 0.068
2.238AlaPro: 2.238 ± 0.063
2.508AlaGln: 2.508 ± 0.046
2.199AlaArg: 2.199 ± 0.053
4.313AlaSer: 4.313 ± 0.077
4.515AlaThr: 4.515 ± 0.1
4.652AlaVal: 4.652 ± 0.075
0.645AlaTrp: 0.645 ± 0.022
2.587AlaTyr: 2.587 ± 0.052
0.0AlaXaa: 0.0 ± 0.0
Cys
0.649CysAla: 0.649 ± 0.031
0.121CysCys: 0.121 ± 0.01
0.519CysAsp: 0.519 ± 0.032
0.559CysGlu: 0.559 ± 0.025
0.476CysPhe: 0.476 ± 0.023
0.803CysGly: 0.803 ± 0.031
0.192CysHis: 0.192 ± 0.015
0.708CysIle: 0.708 ± 0.029
0.472CysLys: 0.472 ± 0.023
0.79CysLeu: 0.79 ± 0.03
0.206CysMet: 0.206 ± 0.015
0.545CysAsn: 0.545 ± 0.025
0.431CysPro: 0.431 ± 0.027
0.347CysGln: 0.347 ± 0.021
0.308CysArg: 0.308 ± 0.019
0.807CysSer: 0.807 ± 0.037
0.705CysThr: 0.705 ± 0.032
0.586CysVal: 0.586 ± 0.022
0.086CysTrp: 0.086 ± 0.009
0.403CysTyr: 0.403 ± 0.021
0.0CysXaa: 0.0 ± 0.0
Asp
3.755AspAla: 3.755 ± 0.063
0.534AspCys: 0.534 ± 0.025
2.414AspAsp: 2.414 ± 0.057
3.543AspGlu: 3.543 ± 0.061
3.12AspPhe: 3.12 ± 0.057
3.994AspGly: 3.994 ± 0.09
0.901AspHis: 0.901 ± 0.032
3.51AspIle: 3.51 ± 0.055
3.282AspLys: 3.282 ± 0.055
4.728AspLeu: 4.728 ± 0.069
1.038AspMet: 1.038 ± 0.03
2.699AspAsn: 2.699 ± 0.065
1.938AspPro: 1.938 ± 0.056
1.774AspGln: 1.774 ± 0.037
1.974AspArg: 1.974 ± 0.043
2.971AspSer: 2.971 ± 0.058
2.697AspThr: 2.697 ± 0.063
3.698AspVal: 3.698 ± 0.063
0.683AspTrp: 0.683 ± 0.024
2.619AspTyr: 2.619 ± 0.055
0.0AspXaa: 0.0 ± 0.0
Glu
4.045GluAla: 4.045 ± 0.081
0.452GluCys: 0.452 ± 0.022
2.945GluAsp: 2.945 ± 0.06
4.708GluGlu: 4.708 ± 0.084
2.69GluPhe: 2.69 ± 0.046
3.705GluGly: 3.705 ± 0.067
1.372GluHis: 1.372 ± 0.039
4.98GluIle: 4.98 ± 0.078
5.37GluLys: 5.37 ± 0.083
6.947GluLeu: 6.947 ± 0.107
1.52GluMet: 1.52 ± 0.042
3.853GluAsn: 3.853 ± 0.059
1.561GluPro: 1.561 ± 0.033
3.102GluGln: 3.102 ± 0.059
2.708GluArg: 2.708 ± 0.053
3.25GluSer: 3.25 ± 0.057
3.674GluThr: 3.674 ± 0.066
4.18GluVal: 4.18 ± 0.065
0.773GluTrp: 0.773 ± 0.028
2.528GluTyr: 2.528 ± 0.053
0.0GluXaa: 0.0 ± 0.0
Phe
2.875PheAla: 2.875 ± 0.054
0.511PheCys: 0.511 ± 0.025
2.907PheAsp: 2.907 ± 0.054
3.164PheGlu: 3.164 ± 0.063
2.65PhePhe: 2.65 ± 0.066
3.587PheGly: 3.587 ± 0.061
0.975PheHis: 0.975 ± 0.027
3.66PheIle: 3.66 ± 0.072
2.722PheLys: 2.722 ± 0.056
4.46PheLeu: 4.46 ± 0.084
1.11PheMet: 1.11 ± 0.032
2.84PheAsn: 2.84 ± 0.06
1.889PhePro: 1.889 ± 0.038
1.719PheGln: 1.719 ± 0.044
1.947PheArg: 1.947 ± 0.049
4.123PheSer: 4.123 ± 0.074
3.113PheThr: 3.113 ± 0.066
3.214PheVal: 3.214 ± 0.063
0.527PheTrp: 0.527 ± 0.027
2.114PheTyr: 2.114 ± 0.051
0.0PheXaa: 0.0 ± 0.0
Gly
4.718GlyAla: 4.718 ± 0.088
0.999GlyCys: 0.999 ± 0.05
3.285GlyAsp: 3.285 ± 0.065
3.893GlyGlu: 3.893 ± 0.065
3.557GlyPhe: 3.557 ± 0.06
5.213GlyGly: 5.213 ± 0.128
1.244GlyHis: 1.244 ± 0.034
5.492GlyIle: 5.492 ± 0.081
4.979GlyLys: 4.979 ± 0.085
5.554GlyLeu: 5.554 ± 0.072
1.842GlyMet: 1.842 ± 0.041
4.09GlyAsn: 4.09 ± 0.078
1.419GlyPro: 1.419 ± 0.048
2.279GlyGln: 2.279 ± 0.052
2.19GlyArg: 2.19 ± 0.056
4.5GlySer: 4.5 ± 0.083
5.392GlyThr: 5.392 ± 0.141
4.663GlyVal: 4.663 ± 0.072
0.868GlyTrp: 0.868 ± 0.033
2.916GlyTyr: 2.916 ± 0.058
0.0GlyXaa: 0.0 ± 0.0
His
1.276HisAla: 1.276 ± 0.036
0.195HisCys: 0.195 ± 0.013
0.843HisAsp: 0.843 ± 0.024
1.052HisGlu: 1.052 ± 0.035
1.224HisPhe: 1.224 ± 0.035
1.171HisGly: 1.171 ± 0.033
0.529HisHis: 0.529 ± 0.025
1.431HisIle: 1.431 ± 0.038
0.985HisLys: 0.985 ± 0.033
1.972HisLeu: 1.972 ± 0.05
0.374HisMet: 0.374 ± 0.018
0.894HisAsn: 0.894 ± 0.028
1.126HisPro: 1.126 ± 0.037
0.787HisGln: 0.787 ± 0.028
0.782HisArg: 0.782 ± 0.03
1.268HisSer: 1.268 ± 0.038
1.215HisThr: 1.215 ± 0.031
1.182HisVal: 1.182 ± 0.035
0.256HisTrp: 0.256 ± 0.016
0.958HisTyr: 0.958 ± 0.031
0.0HisXaa: 0.0 ± 0.0
Ile
5.404IleAla: 5.404 ± 0.085
0.824IleCys: 0.824 ± 0.035
4.494IleAsp: 4.494 ± 0.068
4.935IleGlu: 4.935 ± 0.081
2.979IlePhe: 2.979 ± 0.053
5.026IleGly: 5.026 ± 0.077
1.672IleHis: 1.672 ± 0.039
5.024IleIle: 5.024 ± 0.073
4.064IleLys: 4.064 ± 0.064
5.954IleLeu: 5.954 ± 0.084
1.217IleMet: 1.217 ± 0.031
3.997IleAsn: 3.997 ± 0.067
3.372IlePro: 3.372 ± 0.054
2.836IleGln: 2.836 ± 0.047
3.254IleArg: 3.254 ± 0.056
5.065IleSer: 5.065 ± 0.066
4.726IleThr: 4.726 ± 0.081
4.976IleVal: 4.976 ± 0.073
0.689IleTrp: 0.689 ± 0.024
2.764IleTyr: 2.764 ± 0.061
0.0IleXaa: 0.0 ± 0.0
Lys
4.174LysAla: 4.174 ± 0.09
0.345LysCys: 0.345 ± 0.02
3.267LysAsp: 3.267 ± 0.06
5.235LysGlu: 5.235 ± 0.105
2.317LysPhe: 2.317 ± 0.054
4.133LysGly: 4.133 ± 0.064
1.259LysHis: 1.259 ± 0.034
4.836LysIle: 4.836 ± 0.079
5.51LysLys: 5.51 ± 0.096
5.497LysLeu: 5.497 ± 0.08
1.978LysMet: 1.978 ± 0.044
4.016LysAsn: 4.016 ± 0.074
2.054LysPro: 2.054 ± 0.05
2.896LysGln: 2.896 ± 0.059
2.826LysArg: 2.826 ± 0.051
3.559LysSer: 3.559 ± 0.065
3.985LysThr: 3.985 ± 0.062
3.838LysVal: 3.838 ± 0.066
0.818LysTrp: 0.818 ± 0.027
2.526LysTyr: 2.526 ± 0.056
0.0LysXaa: 0.0 ± 0.0
Leu
5.521LeuAla: 5.521 ± 0.079
0.76LeuCys: 0.76 ± 0.031
4.382LeuAsp: 4.382 ± 0.067
5.583LeuGlu: 5.583 ± 0.088
5.049LeuPhe: 5.049 ± 0.094
5.34LeuGly: 5.34 ± 0.08
1.684LeuHis: 1.684 ± 0.042
6.805LeuIle: 6.805 ± 0.103
6.53LeuLys: 6.53 ± 0.092
8.86LeuLeu: 8.86 ± 0.126
2.016LeuMet: 2.016 ± 0.044
5.2LeuAsn: 5.2 ± 0.077
3.637LeuPro: 3.637 ± 0.062
3.226LeuGln: 3.226 ± 0.056
3.352LeuArg: 3.352 ± 0.064
6.708LeuSer: 6.708 ± 0.083
5.81LeuThr: 5.81 ± 0.078
5.431LeuVal: 5.431 ± 0.069
0.793LeuTrp: 0.793 ± 0.032
3.169LeuTyr: 3.169 ± 0.054
0.0LeuXaa: 0.0 ± 0.0
Met
1.507MetAla: 1.507 ± 0.037
0.181MetCys: 0.181 ± 0.015
1.296MetAsp: 1.296 ± 0.036
1.496MetGlu: 1.496 ± 0.039
0.79MetPhe: 0.79 ± 0.025
1.487MetGly: 1.487 ± 0.036
0.421MetHis: 0.421 ± 0.02
1.607MetIle: 1.607 ± 0.044
2.134MetLys: 2.134 ± 0.042
1.831MetLeu: 1.831 ± 0.037
0.576MetMet: 0.576 ± 0.024
1.462MetAsn: 1.462 ± 0.035
0.773MetPro: 0.773 ± 0.03
0.823MetGln: 0.823 ± 0.025
0.961MetArg: 0.961 ± 0.027
1.355MetSer: 1.355 ± 0.036
1.366MetThr: 1.366 ± 0.031
1.249MetVal: 1.249 ± 0.034
0.185MetTrp: 0.185 ± 0.013
0.828MetTyr: 0.828 ± 0.029
0.0MetXaa: 0.0 ± 0.0
Asn
3.843AsnAla: 3.843 ± 0.069
0.558AsnCys: 0.558 ± 0.028
2.835AsnAsp: 2.835 ± 0.057
3.466AsnGlu: 3.466 ± 0.06
2.507AsnPhe: 2.507 ± 0.054
4.832AsnGly: 4.832 ± 0.106
1.089AsnHis: 1.089 ± 0.035
3.537AsnIle: 3.537 ± 0.06
3.243AsnLys: 3.243 ± 0.056
4.554AsnLeu: 4.554 ± 0.073
1.195AsnMet: 1.195 ± 0.037
3.277AsnAsn: 3.277 ± 0.078
3.023AsnPro: 3.023 ± 0.073
2.337AsnGln: 2.337 ± 0.046
2.278AsnArg: 2.278 ± 0.047
3.445AsnSer: 3.445 ± 0.068
3.529AsnThr: 3.529 ± 0.068
3.415AsnVal: 3.415 ± 0.068
0.776AsnTrp: 0.776 ± 0.028
2.764AsnTyr: 2.764 ± 0.064
0.0AsnXaa: 0.0 ± 0.0
Pro
2.715ProAla: 2.715 ± 0.066
0.364ProCys: 0.364 ± 0.023
2.307ProAsp: 2.307 ± 0.045
2.905ProGlu: 2.905 ± 0.061
2.013ProPhe: 2.013 ± 0.041
2.644ProGly: 2.644 ± 0.063
0.688ProHis: 0.688 ± 0.024
2.279ProIle: 2.279 ± 0.044
2.074ProLys: 2.074 ± 0.049
2.988ProLeu: 2.988 ± 0.049
0.754ProMet: 0.754 ± 0.027
2.143ProAsn: 2.143 ± 0.054
0.954ProPro: 0.954 ± 0.038
1.205ProGln: 1.205 ± 0.035
0.948ProArg: 0.948 ± 0.03
2.356ProSer: 2.356 ± 0.047
2.266ProThr: 2.266 ± 0.062
3.387ProVal: 3.387 ± 0.079
0.334ProTrp: 0.334 ± 0.016
1.508ProTyr: 1.508 ± 0.038
0.0ProXaa: 0.0 ± 0.0
Gln
2.256GlnAla: 2.256 ± 0.043
0.252GlnCys: 0.252 ± 0.017
1.579GlnAsp: 1.579 ± 0.039
2.465GlnGlu: 2.465 ± 0.053
1.882GlnPhe: 1.882 ± 0.047
2.084GlnGly: 2.084 ± 0.055
0.858GlnHis: 0.858 ± 0.03
2.501GlnIle: 2.501 ± 0.047
2.53GlnLys: 2.53 ± 0.061
4.515GlnLeu: 4.515 ± 0.075
0.884GlnMet: 0.884 ± 0.029
1.943GlnAsn: 1.943 ± 0.051
1.464GlnPro: 1.464 ± 0.044
2.108GlnGln: 2.108 ± 0.064
1.496GlnArg: 1.496 ± 0.041
2.342GlnSer: 2.342 ± 0.049
2.318GlnThr: 2.318 ± 0.05
2.352GlnVal: 2.352 ± 0.045
0.492GlnTrp: 0.492 ± 0.023
1.473GlnTyr: 1.473 ± 0.038
0.0GlnXaa: 0.0 ± 0.0
Arg
2.026ArgAla: 2.026 ± 0.039
0.245ArgCys: 0.245 ± 0.015
1.758ArgAsp: 1.758 ± 0.043
2.522ArgGlu: 2.522 ± 0.061
2.12ArgPhe: 2.12 ± 0.049
1.875ArgGly: 1.875 ± 0.049
0.717ArgHis: 0.717 ± 0.028
3.231ArgIle: 3.231 ± 0.06
2.988ArgLys: 2.988 ± 0.062
3.71ArgLeu: 3.71 ± 0.067
1.159ArgMet: 1.159 ± 0.038
2.133ArgAsn: 2.133 ± 0.049
1.238ArgPro: 1.238 ± 0.04
1.418ArgGln: 1.418 ± 0.037
1.387ArgArg: 1.387 ± 0.039
2.181ArgSer: 2.181 ± 0.045
2.136ArgThr: 2.136 ± 0.05
2.342ArgVal: 2.342 ± 0.045
0.461ArgTrp: 0.461 ± 0.021
1.697ArgTyr: 1.697 ± 0.042
0.0ArgXaa: 0.0 ± 0.0
Ser
4.016SerAla: 4.016 ± 0.076
0.745SerCys: 0.745 ± 0.032
3.435SerAsp: 3.435 ± 0.056
3.863SerGlu: 3.863 ± 0.061
3.693SerPhe: 3.693 ± 0.067
5.616SerGly: 5.616 ± 0.104
1.142SerHis: 1.142 ± 0.034
4.873SerIle: 4.873 ± 0.074
3.655SerLys: 3.655 ± 0.075
5.673SerLeu: 5.673 ± 0.087
1.375SerMet: 1.375 ± 0.037
3.583SerAsn: 3.583 ± 0.073
2.401SerPro: 2.401 ± 0.056
2.129SerGln: 2.129 ± 0.051
2.188SerArg: 2.188 ± 0.04
4.523SerSer: 4.523 ± 0.093
4.182SerThr: 4.182 ± 0.088
4.791SerVal: 4.791 ± 0.079
0.803SerTrp: 0.803 ± 0.029
2.828SerTyr: 2.828 ± 0.048
0.0SerXaa: 0.0 ± 0.0
Thr
4.865ThrAla: 4.865 ± 0.108
0.574ThrCys: 0.574 ± 0.031
3.519ThrAsp: 3.519 ± 0.071
3.621ThrGlu: 3.621 ± 0.056
3.189ThrPhe: 3.189 ± 0.066
5.163ThrGly: 5.163 ± 0.103
1.203ThrHis: 1.203 ± 0.035
5.317ThrIle: 5.317 ± 0.076
3.076ThrLys: 3.076 ± 0.057
5.411ThrLeu: 5.411 ± 0.069
1.097ThrMet: 1.097 ± 0.032
3.348ThrAsn: 3.348 ± 0.072
2.971ThrPro: 2.971 ± 0.079
2.014ThrGln: 2.014 ± 0.052
1.85ThrArg: 1.85 ± 0.043
4.239ThrSer: 4.239 ± 0.086
4.712ThrThr: 4.712 ± 0.118
4.97ThrVal: 4.97 ± 0.11
0.719ThrTrp: 0.719 ± 0.028
2.834ThrTyr: 2.834 ± 0.08
0.0ThrXaa: 0.0 ± 0.0
Val
4.445ValAla: 4.445 ± 0.078
0.723ValCys: 0.723 ± 0.027
3.43ValAsp: 3.43 ± 0.061
4.206ValGlu: 4.206 ± 0.071
3.564ValPhe: 3.564 ± 0.061
3.895ValGly: 3.895 ± 0.072
1.283ValHis: 1.283 ± 0.035
4.998ValIle: 4.998 ± 0.073
4.132ValLys: 4.132 ± 0.073
5.981ValLeu: 5.981 ± 0.076
1.361ValMet: 1.361 ± 0.039
3.941ValAsn: 3.941 ± 0.069
2.459ValPro: 2.459 ± 0.052
2.23ValGln: 2.23 ± 0.05
2.483ValArg: 2.483 ± 0.045
4.665ValSer: 4.665 ± 0.077
4.579ValThr: 4.579 ± 0.117
4.838ValVal: 4.838 ± 0.093
0.697ValTrp: 0.697 ± 0.026
2.897ValTyr: 2.897 ± 0.053
0.0ValXaa: 0.0 ± 0.0
Trp
0.614TrpAla: 0.614 ± 0.024
0.118TrpCys: 0.118 ± 0.01
0.715TrpAsp: 0.715 ± 0.029
0.693TrpGlu: 0.693 ± 0.027
0.548TrpPhe: 0.548 ± 0.024
0.796TrpGly: 0.796 ± 0.03
0.224TrpHis: 0.224 ± 0.013
0.827TrpIle: 0.827 ± 0.027
0.888TrpLys: 0.888 ± 0.032
0.915TrpLeu: 0.915 ± 0.035
0.356TrpMet: 0.356 ± 0.018
0.803TrpAsn: 0.803 ± 0.036
0.22TrpPro: 0.22 ± 0.016
0.386TrpGln: 0.386 ± 0.018
0.372TrpArg: 0.372 ± 0.02
0.793TrpSer: 0.793 ± 0.033
0.665TrpThr: 0.665 ± 0.026
0.662TrpVal: 0.662 ± 0.025
0.145TrpTrp: 0.145 ± 0.011
0.497TrpTyr: 0.497 ± 0.024
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.6TyrAla: 2.6 ± 0.047
0.441TyrCys: 0.441 ± 0.023
2.365TyrAsp: 2.365 ± 0.059
2.379TyrGlu: 2.379 ± 0.046
2.493TyrPhe: 2.493 ± 0.046
2.676TyrGly: 2.676 ± 0.062
0.892TyrHis: 0.892 ± 0.033
2.581TyrIle: 2.581 ± 0.054
2.371TyrLys: 2.371 ± 0.05
3.677TyrLeu: 3.677 ± 0.055
0.793TyrMet: 0.793 ± 0.029
2.442TyrAsn: 2.442 ± 0.055
1.666TyrPro: 1.666 ± 0.04
1.668TyrGln: 1.668 ± 0.039
1.841TyrArg: 1.841 ± 0.045
3.053TyrSer: 3.053 ± 0.062
3.103TyrThr: 3.103 ± 0.078
2.382TyrVal: 2.382 ± 0.047
0.488TyrTrp: 0.488 ± 0.022
2.072TyrTyr: 2.072 ± 0.06
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3167 proteins (1122623 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski