Cronobacter phage vB_CsaM_GAP31

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Vequintavirinae; Seunavirus; Cronobacter virus GAP31

Average proteome isoelectric point is 6.34

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 269 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K4F6N3|K4F6N3_9CAUD Uncharacterized protein OS=Cronobacter phage vB_CsaM_GAP31 OX=1141135 GN=GAP31_076 PE=4 SV=1
MM1 pKa = 7.56EE2 pKa = 6.04LSDD5 pKa = 5.14LYY7 pKa = 11.29SDD9 pKa = 3.77APIRR13 pKa = 11.84LSPAEE18 pKa = 4.33YY19 pKa = 10.76KK20 pKa = 10.8NADD23 pKa = 3.63LVKK26 pKa = 10.25HH27 pKa = 4.96VVAMEE32 pKa = 3.96FEE34 pKa = 4.24FFEE37 pKa = 4.4YY38 pKa = 10.5LASFEE43 pKa = 5.92DD44 pKa = 4.52DD45 pKa = 3.89FNSDD49 pKa = 3.19GLYY52 pKa = 10.72GAA54 pKa = 5.36

Molecular weight: 6.17 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K4F5T7|K4F5T7_9CAUD Uncharacterized protein OS=Cronobacter phage vB_CsaM_GAP31 OX=1141135 GN=GAP31_077 PE=4 SV=1
MM1 pKa = 7.38NNYY4 pKa = 9.57FNSRR8 pKa = 11.84ALARR12 pKa = 11.84TAAKK16 pKa = 10.67SNGGKK21 pKa = 10.56VIDD24 pKa = 4.81NGTAAPTGKK33 pKa = 9.25RR34 pKa = 11.84WEE36 pKa = 4.32VIPGVMVEE44 pKa = 4.99AIQDD48 pKa = 3.73TADD51 pKa = 3.06QRR53 pKa = 11.84AEE55 pKa = 3.92MEE57 pKa = 4.13RR58 pKa = 11.84APVVTLEE65 pKa = 4.11VKK67 pKa = 10.27AGYY70 pKa = 9.94RR71 pKa = 11.84NRR73 pKa = 11.84KK74 pKa = 7.95APRR77 pKa = 11.84VSYY80 pKa = 11.12VHH82 pKa = 7.21DD83 pKa = 4.74ANGKK87 pKa = 8.25PIPVMHH93 pKa = 6.95KK94 pKa = 9.45RR95 pKa = 11.84SQVAARR101 pKa = 11.84LAAHH105 pKa = 7.69LARR108 pKa = 11.84NN109 pKa = 3.77

Molecular weight: 11.91 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

269

0

269

44952

37

929

167.1

18.85

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.25 ± 0.206

1.29 ± 0.068

6.543 ± 0.131

6.576 ± 0.215

4.26 ± 0.113

7.094 ± 0.224

1.849 ± 0.088

5.764 ± 0.097

6.841 ± 0.257

7.833 ± 0.149

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.994 ± 0.101

4.774 ± 0.136

3.806 ± 0.133

3.464 ± 0.117

4.923 ± 0.149

5.864 ± 0.161

6.006 ± 0.222

7.159 ± 0.169

1.789 ± 0.091

3.922 ± 0.136
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski