Aurantimonas sp. Leaf443
Average proteome isoelectric point is 6.48
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3439 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0Q6F8A7|A0A0Q6F8A7_9RHIZ Cytochrome o ubiquinol oxidase subunit I OS=Aurantimonas sp. Leaf443 OX=1736378 GN=ASG48_17435 PE=3 SV=1
MM1 pKa = 7.41 KK2 pKa = 10.47 RR3 pKa = 11.84 LALLLASAAFVTPALAADD21 pKa = 4.54 VIYY24 pKa = 7.59 DD25 pKa = 3.9 TPVAPAAPMEE35 pKa = 4.31 IAPVTEE41 pKa = 4.29 TNTWTGLYY49 pKa = 9.84 IGGQAGVAFGQDD61 pKa = 3.2 SNDD64 pKa = 2.9 IRR66 pKa = 11.84 FDD68 pKa = 3.67 PEE70 pKa = 3.65 NNGAFGEE77 pKa = 4.76 TIFEE81 pKa = 4.38 NDD83 pKa = 3.18 DD84 pKa = 3.49 TNAGFVGGGHH94 pKa = 6.88 IGYY97 pKa = 9.89 DD98 pKa = 3.44 YY99 pKa = 11.4 QFGNNLVVGAVADD112 pKa = 4.13 LNYY115 pKa = 10.06 IDD117 pKa = 5.69 AEE119 pKa = 4.32 TSRR122 pKa = 11.84 SYY124 pKa = 11.25 SIDD127 pKa = 3.0 GNRR130 pKa = 11.84 FGVSEE135 pKa = 4.26 DD136 pKa = 3.21 LDD138 pKa = 3.87 YY139 pKa = 11.4 FGTVRR144 pKa = 11.84 GRR146 pKa = 11.84 LGYY149 pKa = 11.2 AMDD152 pKa = 5.12 SILVYY157 pKa = 10.79 GSGGLAYY164 pKa = 10.35 SGYY167 pKa = 10.56 NRR169 pKa = 11.84 DD170 pKa = 3.11 NSFPSVPTNSLAGYY184 pKa = 6.41 TFKK187 pKa = 11.12 EE188 pKa = 4.59 DD189 pKa = 3.61 GDD191 pKa = 4.35 DD192 pKa = 3.6 VDD194 pKa = 3.82 VGYY197 pKa = 10.86 SVGGGIDD204 pKa = 3.48 VMATRR209 pKa = 11.84 NISFGVEE216 pKa = 3.57 YY217 pKa = 10.34 LYY219 pKa = 11.18 TNLGKK224 pKa = 10.02 NDD226 pKa = 3.67 YY227 pKa = 10.69 SVEE230 pKa = 4.0 ATNGSNTIDD239 pKa = 3.49 LTSTSKK245 pKa = 11.3 DD246 pKa = 3.22 DD247 pKa = 5.04 LDD249 pKa = 3.78 FHH251 pKa = 6.88 TIFAKK256 pKa = 10.43 ASYY259 pKa = 10.27 RR260 pKa = 11.84 FNN262 pKa = 3.56
Molecular weight: 27.99 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.754
IPC2_protein 3.923
IPC_protein 3.948
Toseland 3.719
ProMoST 4.113
Dawson 3.935
Bjellqvist 4.101
Wikipedia 3.897
Rodwell 3.77
Grimsley 3.617
Solomon 3.935
Lehninger 3.897
Nozaki 4.05
DTASelect 4.329
Thurlkill 3.77
EMBOSS 3.897
Sillero 4.062
Patrickios 0.922
IPC_peptide 3.935
IPC2_peptide 4.037
IPC2.peptide.svr19 3.945
Protein with the highest isoelectric point:
>tr|A0A0Q6F141|A0A0Q6F141_9RHIZ GTPase HflX OS=Aurantimonas sp. Leaf443 OX=1736378 GN=hflX PE=3 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 10.27 QPSKK9 pKa = 9.73 LVRR12 pKa = 11.84 KK13 pKa = 9.15 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.42 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATVGGRR28 pKa = 11.84 AVIASRR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.73 GRR39 pKa = 11.84 KK40 pKa = 8.92 RR41 pKa = 11.84 LSAA44 pKa = 3.96
Molecular weight: 5.07 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.431
IPC2_protein 11.125
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.34
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.076
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.079
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3439
0
3439
1115558
41
3002
324.4
34.86
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
14.026 ± 0.067
0.766 ± 0.012
5.569 ± 0.03
6.016 ± 0.036
3.732 ± 0.026
9.043 ± 0.044
1.841 ± 0.018
4.589 ± 0.032
2.621 ± 0.035
10.522 ± 0.063
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.308 ± 0.021
2.071 ± 0.02
5.36 ± 0.032
2.638 ± 0.023
7.867 ± 0.046
5.166 ± 0.031
5.164 ± 0.03
7.607 ± 0.035
1.109 ± 0.016
1.985 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here