Streptomyces phage Abt2graduatex2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Woodruffvirus; unclassified Woodruffvirus

Average proteome isoelectric point is 6.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 71 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2D1GP29|A0A2D1GP29_9CAUD Uncharacterized protein OS=Streptomyces phage Abt2graduatex2 OX=2041353 GN=SEA_ABT2GRADUATEX2_42 PE=4 SV=1
MM1 pKa = 7.39SCDD4 pKa = 4.54LIANLDD10 pKa = 3.64VVRR13 pKa = 11.84ATRR16 pKa = 11.84VDD18 pKa = 3.2NCGAPIPGEE27 pKa = 3.65NAFVSEE33 pKa = 4.63CVASVAMNPNVDD45 pKa = 3.33VADD48 pKa = 3.95DD49 pKa = 4.18VIYY52 pKa = 10.59RR53 pKa = 11.84AANGTLCGVKK63 pKa = 10.19RR64 pKa = 11.84GCPTLLGYY72 pKa = 10.45DD73 pKa = 3.5LEE75 pKa = 5.03FNFFQVSPQLTSVLTNQPEE94 pKa = 4.06VLDD97 pKa = 4.33ANGEE101 pKa = 4.24LVGNDD106 pKa = 3.35DD107 pKa = 5.52CAIQCAGGFALEE119 pKa = 4.85FWAEE123 pKa = 3.95LVGQNCTTTGAQRR136 pKa = 11.84YY137 pKa = 9.18LYY139 pKa = 10.79VLLPWVSNAYY149 pKa = 9.7ISDD152 pKa = 4.01LSIGNEE158 pKa = 3.42AVTFQLVGNSKK169 pKa = 10.8AGGQWGVGPYY179 pKa = 10.52DD180 pKa = 3.69VVDD183 pKa = 3.71TAIAPAPPVAGPMLTPLGDD202 pKa = 3.43TCHH205 pKa = 6.76RR206 pKa = 11.84RR207 pKa = 11.84MQIVTIAPPVPDD219 pKa = 4.64PACDD223 pKa = 3.42FATVPPAEE231 pKa = 4.11

Molecular weight: 24.31 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2D1GNU1|A0A2D1GNU1_9CAUD Uncharacterized protein OS=Streptomyces phage Abt2graduatex2 OX=2041353 GN=SEA_ABT2GRADUATEX2_62 PE=4 SV=1
MM1 pKa = 7.53GMTKK5 pKa = 9.43TPAPKK10 pKa = 9.98YY11 pKa = 10.24LSRR14 pKa = 11.84QEE16 pKa = 3.72AALALGIDD24 pKa = 3.96VPAVDD29 pKa = 4.34RR30 pKa = 11.84LISRR34 pKa = 11.84GLLARR39 pKa = 11.84YY40 pKa = 9.21RR41 pKa = 11.84IRR43 pKa = 11.84GRR45 pKa = 11.84YY46 pKa = 7.82VRR48 pKa = 11.84VPAGQVLEE56 pKa = 4.46LSSLPRR62 pKa = 11.84EE63 pKa = 3.9WLLRR67 pKa = 11.84CC68 pKa = 4.59

Molecular weight: 7.65 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

71

0

71

17280

36

1250

243.4

26.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.715 ± 0.501

1.615 ± 0.221

6.458 ± 0.291

5.145 ± 0.247

2.61 ± 0.173

8.97 ± 0.529

1.985 ± 0.19

3.455 ± 0.24

3.015 ± 0.301

8.27 ± 0.323

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.303 ± 0.178

2.76 ± 0.177

5.666 ± 0.254

3.125 ± 0.143

7.031 ± 0.326

5.394 ± 0.195

6.759 ± 0.212

7.946 ± 0.244

1.707 ± 0.139

2.072 ± 0.169
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski