Amino acid dipepetide frequency for Streptomyces phage Abt2graduatex2

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
23.497AlaAla: 23.497 ± 1.803
1.331AlaCys: 1.331 ± 0.349
8.507AlaAsp: 8.507 ± 0.985
10.359AlaGlu: 10.359 ± 0.765
3.762AlaPhe: 3.762 ± 0.468
11.228AlaGly: 11.228 ± 0.924
2.199AlaHis: 2.199 ± 0.383
3.472AlaIle: 3.472 ± 0.564
3.588AlaLys: 3.588 ± 0.552
10.475AlaLeu: 10.475 ± 1.108
3.993AlaMet: 3.993 ± 0.503
3.646AlaAsn: 3.646 ± 0.556
6.25AlaPro: 6.25 ± 0.693
3.935AlaGln: 3.935 ± 0.432
8.913AlaArg: 8.913 ± 0.851
7.697AlaSer: 7.697 ± 0.765
9.433AlaThr: 9.433 ± 0.724
10.417AlaVal: 10.417 ± 0.912
1.215AlaTrp: 1.215 ± 0.262
3.241AlaTyr: 3.241 ± 0.449
0.0AlaXaa: 0.0 ± 0.0
Cys
2.257CysAla: 2.257 ± 0.389
0.521CysCys: 0.521 ± 0.249
0.752CysAsp: 0.752 ± 0.256
0.694CysGlu: 0.694 ± 0.267
0.405CysPhe: 0.405 ± 0.17
2.431CysGly: 2.431 ± 0.554
0.463CysHis: 0.463 ± 0.174
0.347CysIle: 0.347 ± 0.143
0.289CysLys: 0.289 ± 0.122
1.1CysLeu: 1.1 ± 0.292
0.347CysMet: 0.347 ± 0.165
0.289CysAsn: 0.289 ± 0.15
1.215CysPro: 1.215 ± 0.356
0.521CysGln: 0.521 ± 0.156
1.042CysArg: 1.042 ± 0.275
0.579CysSer: 0.579 ± 0.221
0.868CysThr: 0.868 ± 0.226
1.331CysVal: 1.331 ± 0.287
0.289CysTrp: 0.289 ± 0.117
0.405CysTyr: 0.405 ± 0.166
0.0CysXaa: 0.0 ± 0.0
Asp
8.623AspAla: 8.623 ± 0.623
1.273AspCys: 1.273 ± 0.297
4.804AspAsp: 4.804 ± 0.733
4.514AspGlu: 4.514 ± 0.656
1.678AspPhe: 1.678 ± 0.384
5.498AspGly: 5.498 ± 0.653
1.273AspHis: 1.273 ± 0.267
1.62AspIle: 1.62 ± 0.347
1.215AspLys: 1.215 ± 0.28
6.077AspLeu: 6.077 ± 0.691
1.273AspMet: 1.273 ± 0.318
1.1AspAsn: 1.1 ± 0.194
3.704AspPro: 3.704 ± 0.563
2.199AspGln: 2.199 ± 0.401
5.209AspArg: 5.209 ± 0.596
3.762AspSer: 3.762 ± 0.503
3.299AspThr: 3.299 ± 0.465
5.787AspVal: 5.787 ± 0.654
0.926AspTrp: 0.926 ± 0.299
0.752AspTyr: 0.752 ± 0.203
0.0AspXaa: 0.0 ± 0.0
Glu
8.45GluAla: 8.45 ± 0.753
1.1GluCys: 1.1 ± 0.28
3.183GluAsp: 3.183 ± 0.532
1.273GluGlu: 1.273 ± 0.287
1.447GluPhe: 1.447 ± 0.295
4.63GluGly: 4.63 ± 0.571
1.215GluHis: 1.215 ± 0.278
1.157GluIle: 1.157 ± 0.314
0.926GluLys: 0.926 ± 0.203
5.614GluLeu: 5.614 ± 0.499
1.389GluMet: 1.389 ± 0.269
1.042GluAsn: 1.042 ± 0.235
3.183GluPro: 3.183 ± 0.518
1.852GluGln: 1.852 ± 0.379
4.109GluArg: 4.109 ± 0.568
1.678GluSer: 1.678 ± 0.363
3.53GluThr: 3.53 ± 0.398
3.704GluVal: 3.704 ± 0.427
1.042GluTrp: 1.042 ± 0.257
0.926GluTyr: 0.926 ± 0.283
0.0GluXaa: 0.0 ± 0.0
Phe
3.125PheAla: 3.125 ± 0.403
0.231PheCys: 0.231 ± 0.121
2.141PheAsp: 2.141 ± 0.429
1.678PheGlu: 1.678 ± 0.321
0.637PhePhe: 0.637 ± 0.225
2.894PheGly: 2.894 ± 0.431
0.463PheHis: 0.463 ± 0.165
0.521PheIle: 0.521 ± 0.171
0.463PheLys: 0.463 ± 0.22
2.373PheLeu: 2.373 ± 0.432
0.752PheMet: 0.752 ± 0.206
1.331PheAsn: 1.331 ± 0.298
1.62PhePro: 1.62 ± 0.35
1.042PheGln: 1.042 ± 0.251
1.563PheArg: 1.563 ± 0.306
1.331PheSer: 1.331 ± 0.217
1.62PheThr: 1.62 ± 0.287
1.91PheVal: 1.91 ± 0.333
0.174PheTrp: 0.174 ± 0.092
0.231PheTyr: 0.231 ± 0.1
0.0PheXaa: 0.0 ± 0.0
Gly
8.97GlyAla: 8.97 ± 1.178
2.373GlyCys: 2.373 ± 0.563
4.804GlyAsp: 4.804 ± 0.548
4.514GlyGlu: 4.514 ± 0.505
2.894GlyPhe: 2.894 ± 0.423
9.086GlyGly: 9.086 ± 2.172
1.852GlyHis: 1.852 ± 0.336
3.299GlyIle: 3.299 ± 0.466
3.472GlyLys: 3.472 ± 0.589
7.639GlyLeu: 7.639 ± 0.748
1.62GlyMet: 1.62 ± 0.308
2.489GlyAsn: 2.489 ± 0.449
4.804GlyPro: 4.804 ± 0.624
3.704GlyGln: 3.704 ± 0.528
6.482GlyArg: 6.482 ± 0.654
4.861GlySer: 4.861 ± 0.553
6.366GlyThr: 6.366 ± 0.696
6.25GlyVal: 6.25 ± 0.514
2.141GlyTrp: 2.141 ± 0.36
2.083GlyTyr: 2.083 ± 0.344
0.0GlyXaa: 0.0 ± 0.0
His
2.026HisAla: 2.026 ± 0.312
0.347HisCys: 0.347 ± 0.146
0.984HisAsp: 0.984 ± 0.285
1.042HisGlu: 1.042 ± 0.223
0.579HisPhe: 0.579 ± 0.2
1.62HisGly: 1.62 ± 0.357
0.579HisHis: 0.579 ± 0.185
0.637HisIle: 0.637 ± 0.219
0.463HisLys: 0.463 ± 0.167
1.331HisLeu: 1.331 ± 0.33
0.521HisMet: 0.521 ± 0.213
0.694HisAsn: 0.694 ± 0.212
0.926HisPro: 0.926 ± 0.279
0.579HisGln: 0.579 ± 0.183
2.199HisArg: 2.199 ± 0.373
0.926HisSer: 0.926 ± 0.217
1.273HisThr: 1.273 ± 0.281
1.794HisVal: 1.794 ± 0.371
0.81HisTrp: 0.81 ± 0.257
0.521HisTyr: 0.521 ± 0.169
0.0HisXaa: 0.0 ± 0.0
Ile
4.63IleAla: 4.63 ± 0.587
0.289IleCys: 0.289 ± 0.141
1.563IleAsp: 1.563 ± 0.304
2.836IleGlu: 2.836 ± 0.359
0.579IlePhe: 0.579 ± 0.161
3.125IleGly: 3.125 ± 0.514
0.984IleHis: 0.984 ± 0.231
1.331IleIle: 1.331 ± 0.234
1.1IleLys: 1.1 ± 0.236
2.199IleLeu: 2.199 ± 0.498
0.463IleMet: 0.463 ± 0.156
1.273IleAsn: 1.273 ± 0.324
2.141IlePro: 2.141 ± 0.289
1.215IleGln: 1.215 ± 0.267
2.141IleArg: 2.141 ± 0.371
2.315IleSer: 2.315 ± 0.4
2.836IleThr: 2.836 ± 0.627
2.431IleVal: 2.431 ± 0.388
0.405IleTrp: 0.405 ± 0.153
0.694IleTyr: 0.694 ± 0.227
0.0IleXaa: 0.0 ± 0.0
Lys
3.935LysAla: 3.935 ± 0.427
0.347LysCys: 0.347 ± 0.175
1.968LysAsp: 1.968 ± 0.307
0.81LysGlu: 0.81 ± 0.249
0.347LysPhe: 0.347 ± 0.122
2.72LysGly: 2.72 ± 0.479
1.1LysHis: 1.1 ± 0.24
0.752LysIle: 0.752 ± 0.215
1.215LysLys: 1.215 ± 0.258
2.894LysLeu: 2.894 ± 0.38
1.157LysMet: 1.157 ± 0.333
0.694LysAsn: 0.694 ± 0.2
2.257LysPro: 2.257 ± 0.445
1.273LysGln: 1.273 ± 0.269
1.91LysArg: 1.91 ± 0.32
1.447LysSer: 1.447 ± 0.294
2.373LysThr: 2.373 ± 0.398
1.794LysVal: 1.794 ± 0.354
0.405LysTrp: 0.405 ± 0.143
0.752LysTyr: 0.752 ± 0.263
0.0LysXaa: 0.0 ± 0.0
Leu
11.112LeuAla: 11.112 ± 1.05
1.157LeuCys: 1.157 ± 0.296
6.308LeuAsp: 6.308 ± 0.599
3.588LeuGlu: 3.588 ± 0.457
2.083LeuPhe: 2.083 ± 0.359
6.655LeuGly: 6.655 ± 0.755
1.1LeuHis: 1.1 ± 0.302
3.415LeuIle: 3.415 ± 0.635
3.762LeuLys: 3.762 ± 0.481
6.482LeuLeu: 6.482 ± 0.753
1.736LeuMet: 1.736 ± 0.304
2.373LeuAsn: 2.373 ± 0.38
4.283LeuPro: 4.283 ± 0.484
1.273LeuGln: 1.273 ± 0.229
5.787LeuArg: 5.787 ± 0.679
5.151LeuSer: 5.151 ± 0.582
7.639LeuThr: 7.639 ± 0.85
6.308LeuVal: 6.308 ± 0.555
0.81LeuTrp: 0.81 ± 0.276
1.678LeuTyr: 1.678 ± 0.294
0.0LeuXaa: 0.0 ± 0.0
Met
3.993MetAla: 3.993 ± 0.478
0.116MetCys: 0.116 ± 0.079
1.968MetAsp: 1.968 ± 0.362
0.463MetGlu: 0.463 ± 0.166
0.289MetPhe: 0.289 ± 0.144
1.91MetGly: 1.91 ± 0.383
0.174MetHis: 0.174 ± 0.1
1.273MetIle: 1.273 ± 0.252
0.984MetLys: 0.984 ± 0.24
1.736MetLeu: 1.736 ± 0.311
0.347MetMet: 0.347 ± 0.153
0.868MetAsn: 0.868 ± 0.238
1.157MetPro: 1.157 ± 0.227
0.405MetGln: 0.405 ± 0.144
1.736MetArg: 1.736 ± 0.29
1.331MetSer: 1.331 ± 0.345
1.91MetThr: 1.91 ± 0.451
1.389MetVal: 1.389 ± 0.267
0.463MetTrp: 0.463 ± 0.17
0.521MetTyr: 0.521 ± 0.203
0.0MetXaa: 0.0 ± 0.0
Asn
3.53AsnAla: 3.53 ± 0.527
0.521AsnCys: 0.521 ± 0.195
1.62AsnAsp: 1.62 ± 0.258
1.1AsnGlu: 1.1 ± 0.277
0.81AsnPhe: 0.81 ± 0.221
3.241AsnGly: 3.241 ± 0.419
0.405AsnHis: 0.405 ± 0.151
1.1AsnIle: 1.1 ± 0.256
0.347AsnLys: 0.347 ± 0.149
2.546AsnLeu: 2.546 ± 0.439
0.521AsnMet: 0.521 ± 0.215
0.579AsnAsn: 0.579 ± 0.222
1.852AsnPro: 1.852 ± 0.337
1.331AsnGln: 1.331 ± 0.31
2.026AsnArg: 2.026 ± 0.325
1.1AsnSer: 1.1 ± 0.224
1.389AsnThr: 1.389 ± 0.292
2.431AsnVal: 2.431 ± 0.424
0.463AsnTrp: 0.463 ± 0.175
0.694AsnTyr: 0.694 ± 0.219
0.0AsnXaa: 0.0 ± 0.0
Pro
8.276ProAla: 8.276 ± 0.785
1.1ProCys: 1.1 ± 0.359
4.109ProAsp: 4.109 ± 0.509
3.704ProGlu: 3.704 ± 0.556
1.273ProPhe: 1.273 ± 0.314
5.44ProGly: 5.44 ± 0.741
1.157ProHis: 1.157 ± 0.261
1.447ProIle: 1.447 ± 0.341
2.083ProLys: 2.083 ± 0.433
3.704ProLeu: 3.704 ± 0.613
0.926ProMet: 0.926 ± 0.284
1.1ProAsn: 1.1 ± 0.235
3.357ProPro: 3.357 ± 0.527
1.736ProGln: 1.736 ± 0.326
2.952ProArg: 2.952 ± 0.506
3.53ProSer: 3.53 ± 0.577
2.604ProThr: 2.604 ± 0.424
5.787ProVal: 5.787 ± 0.488
1.331ProTrp: 1.331 ± 0.272
1.042ProTyr: 1.042 ± 0.253
0.0ProXaa: 0.0 ± 0.0
Gln
4.109GlnAla: 4.109 ± 0.485
0.579GlnCys: 0.579 ± 0.173
1.389GlnAsp: 1.389 ± 0.29
0.81GlnGlu: 0.81 ± 0.211
0.868GlnPhe: 0.868 ± 0.242
2.141GlnGly: 2.141 ± 0.327
0.752GlnHis: 0.752 ± 0.206
1.1GlnIle: 1.1 ± 0.241
0.81GlnLys: 0.81 ± 0.222
3.993GlnLeu: 3.993 ± 0.519
0.984GlnMet: 0.984 ± 0.263
0.694GlnAsn: 0.694 ± 0.237
1.852GlnPro: 1.852 ± 0.389
1.447GlnGln: 1.447 ± 0.295
2.604GlnArg: 2.604 ± 0.349
1.447GlnSer: 1.447 ± 0.263
2.026GlnThr: 2.026 ± 0.354
2.026GlnVal: 2.026 ± 0.303
0.926GlnTrp: 0.926 ± 0.229
0.637GlnTyr: 0.637 ± 0.205
0.0GlnXaa: 0.0 ± 0.0
Arg
8.623ArgAla: 8.623 ± 0.699
1.447ArgCys: 1.447 ± 0.382
4.572ArgAsp: 4.572 ± 0.635
2.952ArgGlu: 2.952 ± 0.469
1.678ArgPhe: 1.678 ± 0.31
4.572ArgGly: 4.572 ± 0.558
1.447ArgHis: 1.447 ± 0.343
3.53ArgIle: 3.53 ± 0.443
2.72ArgLys: 2.72 ± 0.682
5.672ArgLeu: 5.672 ± 0.632
2.431ArgMet: 2.431 ± 0.328
2.836ArgAsn: 2.836 ± 0.452
2.836ArgPro: 2.836 ± 0.444
2.141ArgGln: 2.141 ± 0.309
5.44ArgArg: 5.44 ± 0.607
3.241ArgSer: 3.241 ± 0.45
5.093ArgThr: 5.093 ± 0.513
5.672ArgVal: 5.672 ± 0.653
1.389ArgTrp: 1.389 ± 0.294
2.026ArgTyr: 2.026 ± 0.43
0.0ArgXaa: 0.0 ± 0.0
Ser
6.54SerAla: 6.54 ± 0.736
0.868SerCys: 0.868 ± 0.29
3.357SerAsp: 3.357 ± 0.432
3.009SerGlu: 3.009 ± 0.428
1.794SerPhe: 1.794 ± 0.331
7.003SerGly: 7.003 ± 0.728
1.042SerHis: 1.042 ± 0.232
1.505SerIle: 1.505 ± 0.353
1.215SerLys: 1.215 ± 0.269
4.225SerLeu: 4.225 ± 0.511
1.389SerMet: 1.389 ± 0.27
1.273SerAsn: 1.273 ± 0.237
3.125SerPro: 3.125 ± 0.384
1.331SerGln: 1.331 ± 0.355
3.704SerArg: 3.704 ± 0.436
3.183SerSer: 3.183 ± 0.519
3.125SerThr: 3.125 ± 0.407
4.167SerVal: 4.167 ± 0.408
0.868SerTrp: 0.868 ± 0.232
1.215SerTyr: 1.215 ± 0.32
0.0SerXaa: 0.0 ± 0.0
Thr
9.086ThrAla: 9.086 ± 0.797
0.752ThrCys: 0.752 ± 0.251
3.704ThrAsp: 3.704 ± 0.47
4.109ThrGlu: 4.109 ± 0.513
1.852ThrPhe: 1.852 ± 0.356
7.581ThrGly: 7.581 ± 0.609
1.157ThrHis: 1.157 ± 0.282
2.778ThrIle: 2.778 ± 0.428
2.257ThrLys: 2.257 ± 0.359
5.209ThrLeu: 5.209 ± 0.492
1.215ThrMet: 1.215 ± 0.326
1.852ThrAsn: 1.852 ± 0.302
4.341ThrPro: 4.341 ± 0.498
1.678ThrGln: 1.678 ± 0.273
3.762ThrArg: 3.762 ± 0.4
3.762ThrSer: 3.762 ± 0.472
3.241ThrThr: 3.241 ± 0.52
6.655ThrVal: 6.655 ± 0.505
0.984ThrTrp: 0.984 ± 0.246
1.62ThrTyr: 1.62 ± 0.262
0.0ThrXaa: 0.0 ± 0.0
Val
12.269ValAla: 12.269 ± 1.019
1.042ValCys: 1.042 ± 0.298
6.482ValAsp: 6.482 ± 0.717
2.373ValGlu: 2.373 ± 0.408
2.199ValPhe: 2.199 ± 0.335
4.109ValGly: 4.109 ± 0.58
1.389ValHis: 1.389 ± 0.285
4.109ValIle: 4.109 ± 0.609
2.083ValLys: 2.083 ± 0.42
5.787ValLeu: 5.787 ± 0.74
1.331ValMet: 1.331 ± 0.255
2.257ValAsn: 2.257 ± 0.353
5.845ValPro: 5.845 ± 0.586
2.141ValGln: 2.141 ± 0.363
6.077ValArg: 6.077 ± 0.623
4.572ValSer: 4.572 ± 0.492
6.135ValThr: 6.135 ± 0.614
6.655ValVal: 6.655 ± 0.607
1.62ValTrp: 1.62 ± 0.279
0.984ValTyr: 0.984 ± 0.292
0.0ValXaa: 0.0 ± 0.0
Trp
2.083TrpAla: 2.083 ± 0.352
0.405TrpCys: 0.405 ± 0.149
1.1TrpAsp: 1.1 ± 0.245
0.289TrpGlu: 0.289 ± 0.119
0.405TrpPhe: 0.405 ± 0.184
0.868TrpGly: 0.868 ± 0.27
0.463TrpHis: 0.463 ± 0.165
0.289TrpIle: 0.289 ± 0.134
0.637TrpLys: 0.637 ± 0.197
1.794TrpLeu: 1.794 ± 0.329
0.174TrpMet: 0.174 ± 0.091
0.637TrpAsn: 0.637 ± 0.212
1.1TrpPro: 1.1 ± 0.276
0.521TrpGln: 0.521 ± 0.158
0.926TrpArg: 0.926 ± 0.252
1.273TrpSer: 1.273 ± 0.274
1.563TrpThr: 1.563 ± 0.337
1.447TrpVal: 1.447 ± 0.335
0.579TrpTrp: 0.579 ± 0.184
0.521TrpTyr: 0.521 ± 0.189
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.026TyrAla: 2.026 ± 0.364
0.347TyrCys: 0.347 ± 0.145
1.273TyrAsp: 1.273 ± 0.266
1.331TyrGlu: 1.331 ± 0.31
0.521TyrPhe: 0.521 ± 0.19
2.952TyrGly: 2.952 ± 0.49
0.637TyrHis: 0.637 ± 0.189
0.868TyrIle: 0.868 ± 0.285
0.521TyrLys: 0.521 ± 0.179
1.852TyrLeu: 1.852 ± 0.363
0.405TyrMet: 0.405 ± 0.131
0.579TyrAsn: 0.579 ± 0.148
0.81TyrPro: 0.81 ± 0.216
0.926TyrGln: 0.926 ± 0.183
1.736TyrArg: 1.736 ± 0.316
0.752TyrSer: 0.752 ± 0.176
1.273TyrThr: 1.273 ± 0.32
1.505TyrVal: 1.505 ± 0.319
0.231TyrTrp: 0.231 ± 0.153
0.174TyrTyr: 0.174 ± 0.097
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 71 proteins (17280 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski