Streptococcus phage Javan261

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 31 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6A6D8|A0A4D6A6D8_9CAUD Putative head-tail adaptor OS=Streptococcus phage Javan261 OX=2548078 GN=Javan261_0014 PE=4 SV=1
MM1 pKa = 7.47SGYY4 pKa = 8.89ATARR8 pKa = 11.84QNGWMSANDD17 pKa = 3.03IRR19 pKa = 11.84ALEE22 pKa = 4.08NLDD25 pKa = 5.16LISKK29 pKa = 10.14EE30 pKa = 4.26DD31 pKa = 3.62GGDD34 pKa = 3.24LYY36 pKa = 11.13LINGNMLPLKK46 pKa = 10.45DD47 pKa = 3.95AGAFAKK53 pKa = 10.34KK54 pKa = 10.29GEE56 pKa = 4.29HH57 pKa = 5.66NEE59 pKa = 4.03EE60 pKa = 4.09VLEE63 pKa = 5.17LEE65 pKa = 5.76DD66 pKa = 4.83DD67 pKa = 4.07TKK69 pKa = 11.21QQ70 pKa = 3.29

Molecular weight: 7.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6A6G4|A0A4D6A6G4_9CAUD Portal protein OS=Streptococcus phage Javan261 OX=2548078 GN=Javan261_0009 PE=4 SV=1
MM1 pKa = 6.96KK2 pKa = 10.13QIRR5 pKa = 11.84KK6 pKa = 9.35IIQIRR11 pKa = 11.84LPEE14 pKa = 4.02QKK16 pKa = 9.88KK17 pKa = 10.76LKK19 pKa = 8.17VAAYY23 pKa = 10.11ARR25 pKa = 11.84VSHH28 pKa = 5.99TRR30 pKa = 11.84LLQSLSNQVSYY41 pKa = 11.46YY42 pKa = 10.04NQIIGKK48 pKa = 8.38NPEE51 pKa = 3.7WEE53 pKa = 4.32FKK55 pKa = 10.44GVYY58 pKa = 8.84VDD60 pKa = 4.13EE61 pKa = 5.13VISGRR66 pKa = 11.84NASKK70 pKa = 10.2RR71 pKa = 11.84KK72 pKa = 9.0EE73 pKa = 4.03YY74 pKa = 10.53QRR76 pKa = 11.84LLSDD80 pKa = 3.59CRR82 pKa = 11.84KK83 pKa = 10.28GEE85 pKa = 3.84VDD87 pKa = 4.25IILTKK92 pKa = 10.5SISRR96 pKa = 11.84FGRR99 pKa = 11.84NTVEE103 pKa = 3.61LLKK106 pKa = 10.1TIRR109 pKa = 11.84EE110 pKa = 4.11LKK112 pKa = 9.63QLNISVRR119 pKa = 11.84FEE121 pKa = 4.05KK122 pKa = 10.93EE123 pKa = 3.82NIDD126 pKa = 3.69TLTTDD131 pKa = 3.91GEE133 pKa = 4.46LLLTLLSTLAEE144 pKa = 4.31EE145 pKa = 4.16EE146 pKa = 4.35SKK148 pKa = 11.09AISEE152 pKa = 4.2NIKK155 pKa = 10.18WKK157 pKa = 10.24VKK159 pKa = 10.21KK160 pKa = 10.27QFEE163 pKa = 4.11QGLPYY168 pKa = 10.1IKK170 pKa = 9.77QDD172 pKa = 2.99MYY174 pKa = 11.42GYY176 pKa = 10.21RR177 pKa = 11.84FKK179 pKa = 9.96EE180 pKa = 4.03TKK182 pKa = 10.55YY183 pKa = 10.16EE184 pKa = 3.94IEE186 pKa = 3.9PRR188 pKa = 11.84EE189 pKa = 4.17AEE191 pKa = 4.32VVKK194 pKa = 10.41QVYY197 pKa = 8.98AWYY200 pKa = 10.67LEE202 pKa = 4.5GEE204 pKa = 4.38QPTFIARR211 pKa = 11.84KK212 pKa = 9.72LNEE215 pKa = 3.91QGIKK219 pKa = 7.49TRR221 pKa = 11.84KK222 pKa = 8.5GNRR225 pKa = 11.84FSRR228 pKa = 11.84SIIHH232 pKa = 7.3RR233 pKa = 11.84ILGQEE238 pKa = 4.26AYY240 pKa = 9.36TGTLVLQKK248 pKa = 9.22TYY250 pKa = 10.85HH251 pKa = 5.96VGHH254 pKa = 7.31KK255 pKa = 10.11GRR257 pKa = 11.84SVEE260 pKa = 4.27NKK262 pKa = 10.21GEE264 pKa = 3.9RR265 pKa = 11.84TKK267 pKa = 11.34YY268 pKa = 9.51IVEE271 pKa = 4.24NAHH274 pKa = 5.61EE275 pKa = 4.68AIISRR280 pKa = 11.84EE281 pKa = 3.93MFEE284 pKa = 4.82EE285 pKa = 4.3VQQEE289 pKa = 4.0KK290 pKa = 10.69ARR292 pKa = 11.84RR293 pKa = 11.84SLHH296 pKa = 5.27NKK298 pKa = 8.77KK299 pKa = 10.44KK300 pKa = 10.27EE301 pKa = 3.98GRR303 pKa = 11.84HH304 pKa = 5.5DD305 pKa = 3.34

Molecular weight: 35.86 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

31

0

31

7746

54

597

249.9

28.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.235 ± 0.504

0.981 ± 0.16

5.203 ± 0.448

7.449 ± 0.512

3.783 ± 0.237

6.791 ± 0.438

1.975 ± 0.205

6.894 ± 0.414

7.643 ± 0.506

8.817 ± 0.516

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.634 ± 0.266

5.267 ± 0.218

2.969 ± 0.276

4.209 ± 0.203

4.299 ± 0.353

6.765 ± 0.421

6.636 ± 0.574

6.39 ± 0.293

1.33 ± 0.169

3.731 ± 0.3
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski