Flavonifractor plautii ATCC 29863
Average proteome isoelectric point is 6.34
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4278 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|G9YSZ6|G9YSZ6_FLAPL Uncharacterized protein OS=Flavonifractor plautii ATCC 29863 OX=411475 GN=HMPREF0372_02655 PE=4 SV=1
MM1 pKa = 7.28 KK2 pKa = 10.01 RR3 pKa = 11.84 YY4 pKa = 9.47 KK5 pKa = 10.2 RR6 pKa = 11.84 LYY8 pKa = 9.49 VVLAVLLAACIATFALSRR26 pKa = 11.84 YY27 pKa = 8.67 KK28 pKa = 10.46 AQKK31 pKa = 9.93 EE32 pKa = 4.03 QIQTSGEE39 pKa = 4.28 TILSISADD47 pKa = 3.71 EE48 pKa = 4.44 VEE50 pKa = 4.51 SLSWDD55 pKa = 3.36 YY56 pKa = 11.76 DD57 pKa = 3.54 GQTLSFHH64 pKa = 7.47 RR65 pKa = 11.84 DD66 pKa = 3.33 GAWSYY71 pKa = 12.05 DD72 pKa = 3.51 DD73 pKa = 5.67 DD74 pKa = 4.57 AAFPVDD80 pKa = 3.75 AEE82 pKa = 4.25 QMNLLLEE89 pKa = 4.26 PFAAFGAAFIIEE101 pKa = 4.49 DD102 pKa = 3.89 VDD104 pKa = 4.92 DD105 pKa = 4.69 LGQYY109 pKa = 11.01 GLDD112 pKa = 3.57 NPVCTIRR119 pKa = 11.84 ITAGGEE125 pKa = 3.78 THH127 pKa = 7.53 TIQLGDD133 pKa = 3.94 YY134 pKa = 10.65 SKK136 pKa = 10.58 MDD138 pKa = 3.28 AQRR141 pKa = 11.84 YY142 pKa = 7.44 VSIGDD147 pKa = 3.6 GNVYY151 pKa = 10.24 LAAHH155 pKa = 7.02 DD156 pKa = 4.58 PLDD159 pKa = 4.17 EE160 pKa = 4.76 FDD162 pKa = 3.63 VTLDD166 pKa = 3.66 EE167 pKa = 6.83 LIDD170 pKa = 3.82 QDD172 pKa = 5.31 DD173 pKa = 3.89 IPAFGQVSEE182 pKa = 4.68 LRR184 pKa = 11.84 FSGAEE189 pKa = 3.9 NYY191 pKa = 9.78 AVSYY195 pKa = 10.98 QEE197 pKa = 5.66 DD198 pKa = 3.51 GGNTYY203 pKa = 10.6 RR204 pKa = 11.84 EE205 pKa = 3.89 DD206 pKa = 3.22 DD207 pKa = 3.91 VYY209 pKa = 10.48 FTEE212 pKa = 5.16 RR213 pKa = 11.84 DD214 pKa = 3.64 GAQVPLDD221 pKa = 3.68 PDD223 pKa = 3.21 RR224 pKa = 11.84 VEE226 pKa = 6.06 DD227 pKa = 3.87 YY228 pKa = 10.99 LQAIQSLSLTDD239 pKa = 3.71 CVTYY243 pKa = 10.59 SANDD247 pKa = 4.67 DD248 pKa = 3.67 ISSPAAWTAPSS259 pKa = 3.32
Molecular weight: 28.66 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.699
IPC2_protein 3.783
IPC_protein 3.808
Toseland 3.579
ProMoST 3.973
Dawson 3.808
Bjellqvist 3.961
Wikipedia 3.745
Rodwell 3.63
Grimsley 3.49
Solomon 3.795
Lehninger 3.757
Nozaki 3.923
DTASelect 4.177
Thurlkill 3.643
EMBOSS 3.757
Sillero 3.935
Patrickios 0.973
IPC_peptide 3.795
IPC2_peptide 3.91
IPC2.peptide.svr19 3.816
Protein with the highest isoelectric point:
>tr|G9YTJ7|G9YTJ7_FLAPL Uncharacterized protein OS=Flavonifractor plautii ATCC 29863 OX=411475 GN=HMPREF0372_02857 PE=4 SV=1
MM1 pKa = 7.5 KK2 pKa = 10.12 KK3 pKa = 9.97 FHH5 pKa = 5.45 VTRR8 pKa = 11.84 KK9 pKa = 7.37 TVLTIVGVTLPLTAAAILLRR29 pKa = 11.84 RR30 pKa = 11.84 LKK32 pKa = 10.08 RR33 pKa = 11.84 SRR35 pKa = 11.84 RR36 pKa = 11.84 ARR38 pKa = 11.84 SVRR41 pKa = 11.84 AA42 pKa = 3.42
Molecular weight: 4.79 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.493
IPC2_protein 11.096
IPC_protein 12.691
Toseland 12.852
ProMoST 13.349
Dawson 12.852
Bjellqvist 12.852
Wikipedia 13.334
Rodwell 12.544
Grimsley 12.896
Solomon 13.349
Lehninger 13.247
Nozaki 12.852
DTASelect 12.852
Thurlkill 12.852
EMBOSS 13.349
Sillero 12.852
Patrickios 12.281
IPC_peptide 13.349
IPC2_peptide 12.34
IPC2.peptide.svr19 9.118
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4278
0
4278
1149482
37
3468
268.7
29.55
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.234 ± 0.052
1.615 ± 0.02
5.35 ± 0.037
6.85 ± 0.042
3.681 ± 0.029
8.395 ± 0.04
1.834 ± 0.021
5.085 ± 0.034
4.169 ± 0.045
10.394 ± 0.058
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.639 ± 0.02
3.058 ± 0.026
4.558 ± 0.031
3.388 ± 0.024
6.191 ± 0.045
5.319 ± 0.035
5.477 ± 0.039
7.231 ± 0.033
1.155 ± 0.014
3.377 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here