Thiocapsa marina 5811
Average proteome isoelectric point is 6.3
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4915 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|F9UDC1|F9UDC1_9GAMM Sigma54 specific transcriptional regulator Fis family OS=Thiocapsa marina 5811 OX=768671 GN=ThimaDRAFT_2924 PE=4 SV=1
MM1 pKa = 7.07 YY2 pKa = 9.98 RR3 pKa = 11.84 IKK5 pKa = 10.84 HH6 pKa = 5.8 PADD9 pKa = 3.96 PSLVLAALTSGLLVASAAPSWAYY32 pKa = 10.7 DD33 pKa = 3.32 VTDD36 pKa = 3.85 NFSIGAVLGAAGQCQEE52 pKa = 4.25 GLGGLVDD59 pKa = 3.77 EE60 pKa = 5.67 AGQSLEE66 pKa = 4.17 GDD68 pKa = 3.89 TCRR71 pKa = 11.84 GGMPFQLEE79 pKa = 4.03 MSFRR83 pKa = 11.84 PSEE86 pKa = 3.95 RR87 pKa = 11.84 DD88 pKa = 2.91 EE89 pKa = 5.31 LYY91 pKa = 11.07 ALLGFAVDD99 pKa = 3.55 NGLNAVSPFVLAPWAVDD116 pKa = 5.15 LEE118 pKa = 5.72 DD119 pKa = 5.52 DD120 pKa = 4.26 LQDD123 pKa = 3.14 INGRR127 pKa = 11.84 GRR129 pKa = 11.84 DD130 pKa = 3.68 YY131 pKa = 11.45 LLQAWYY137 pKa = 10.01 KK138 pKa = 8.84 HH139 pKa = 4.88 TFTLGEE145 pKa = 4.4 DD146 pKa = 3.39 ATLGATFGIIDD157 pKa = 3.57 STGYY161 pKa = 10.79 LDD163 pKa = 3.76 EE164 pKa = 4.36 NAYY167 pKa = 10.82 ANDD170 pKa = 3.58 EE171 pKa = 4.16 FTQFMNEE178 pKa = 3.6 AFVNSGGFNLPSYY191 pKa = 10.55 DD192 pKa = 3.25 AGAALEE198 pKa = 4.25 LALGDD203 pKa = 3.89 WEE205 pKa = 4.2 ITAAALNIGEE215 pKa = 4.56 NDD217 pKa = 3.99 DD218 pKa = 3.82 GNNYY222 pKa = 9.33 NFWGAQLGYY231 pKa = 10.7 RR232 pKa = 11.84 LDD234 pKa = 3.7 TALGEE239 pKa = 4.31 GNYY242 pKa = 9.92 RR243 pKa = 11.84 AVITGTSSAFFKK255 pKa = 10.74 PGAPLGEE262 pKa = 4.22 EE263 pKa = 4.36 PEE265 pKa = 4.35 GTEE268 pKa = 5.07 DD269 pKa = 4.13 GSPLAAEE276 pKa = 4.48 DD277 pKa = 4.25 TPEE280 pKa = 4.05 KK281 pKa = 9.48 TDD283 pKa = 3.21 LMGYY287 pKa = 9.96 GLSFDD292 pKa = 3.54 QALGDD297 pKa = 3.58 RR298 pKa = 11.84 LGAFLRR304 pKa = 11.84 MSWTDD309 pKa = 3.28 HH310 pKa = 6.52 EE311 pKa = 6.2 DD312 pKa = 3.16 ILDD315 pKa = 3.64 YY316 pKa = 11.01 RR317 pKa = 11.84 GLYY320 pKa = 9.2 TGGLNIGGGLWGRR333 pKa = 11.84 EE334 pKa = 3.64 ADD336 pKa = 3.77 NVGIAYY342 pKa = 9.61 GYY344 pKa = 10.86 LDD346 pKa = 3.95 GANTGIANTNVAEE359 pKa = 4.09 IYY361 pKa = 10.75 YY362 pKa = 10.2 RR363 pKa = 11.84 FAANDD368 pKa = 3.74 FLALTADD375 pKa = 3.69 VQYY378 pKa = 10.55 MADD381 pKa = 3.97 AYY383 pKa = 11.0 DD384 pKa = 3.53 SGEE387 pKa = 4.89 DD388 pKa = 3.25 IEE390 pKa = 5.27 GWIFGLRR397 pKa = 11.84 LVAGLL402 pKa = 3.94
Molecular weight: 43.01 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.711
IPC2_protein 3.757
IPC_protein 3.783
Toseland 3.567
ProMoST 3.935
Dawson 3.77
Bjellqvist 3.923
Wikipedia 3.694
Rodwell 3.605
Grimsley 3.465
Solomon 3.757
Lehninger 3.719
Nozaki 3.872
DTASelect 4.113
Thurlkill 3.605
EMBOSS 3.706
Sillero 3.897
Patrickios 0.858
IPC_peptide 3.757
IPC2_peptide 3.884
IPC2.peptide.svr19 3.796
Protein with the highest isoelectric point:
>tr|F9UCF1|F9UCF1_9GAMM ATP synthase subunit alpha OS=Thiocapsa marina 5811 OX=768671 GN=atpA PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.56 RR3 pKa = 11.84 TFQPSRR9 pKa = 11.84 LKK11 pKa = 10.48 RR12 pKa = 11.84 ARR14 pKa = 11.84 THH16 pKa = 5.93 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 SATKK25 pKa = 10.29 NGRR28 pKa = 11.84 KK29 pKa = 9.22 VLNARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.49 GRR39 pKa = 11.84 VRR41 pKa = 11.84 LATT44 pKa = 3.61
Molecular weight: 5.11 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.676
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.398
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.177
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4915
0
4915
1589608
30
2892
323.4
35.44
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.571 ± 0.046
1.01 ± 0.013
6.032 ± 0.029
6.189 ± 0.031
3.472 ± 0.021
8.21 ± 0.031
2.169 ± 0.017
4.887 ± 0.021
2.533 ± 0.023
11.177 ± 0.048
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.193 ± 0.016
2.285 ± 0.019
5.567 ± 0.028
3.274 ± 0.022
8.147 ± 0.041
5.272 ± 0.023
5.053 ± 0.024
7.182 ± 0.032
1.447 ± 0.015
2.33 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here