Pedobacter psychrophilus

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Sphingobacteriia; Sphingobacteriales; Sphingobacteriaceae; Pedobacter

Average proteome isoelectric point is 6.86

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3388 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A179DMK0|A0A179DMK0_9SPHI Crp/Fnr family transcriptional regulator OS=Pedobacter psychrophilus OX=1826909 GN=A5893_04120 PE=4 SV=1
MM1 pKa = 7.61EE2 pKa = 5.7SISWTTFMITITLLVITYY20 pKa = 7.41YY21 pKa = 10.13IIVFIYY27 pKa = 10.21CYY29 pKa = 10.82GPTLSNILPKK39 pKa = 10.94GSDD42 pKa = 3.53DD43 pKa = 3.65FSNPLFIVDD52 pKa = 4.3DD53 pKa = 4.37DD54 pKa = 4.63EE55 pKa = 5.09EE56 pKa = 4.7VVPEE60 pKa = 4.18NAVEE64 pKa = 4.17TNDD67 pKa = 3.36WQEE70 pKa = 4.2PEE72 pKa = 4.44PEE74 pKa = 4.0IQEE77 pKa = 3.83VDD79 pKa = 3.66SLIEE83 pKa = 3.94EE84 pKa = 4.44LNEE87 pKa = 4.87GIGFAADD94 pKa = 3.87HH95 pKa = 6.99EE96 pKa = 4.47YY97 pKa = 11.08QPLAFKK103 pKa = 10.78AHH105 pKa = 6.76LASIINNFPNLKK117 pKa = 10.45DD118 pKa = 4.46SEE120 pKa = 4.32FQPAINEE127 pKa = 4.6LIVKK131 pKa = 9.28EE132 pKa = 4.34SKK134 pKa = 10.73AYY136 pKa = 10.36GLTGLSEE143 pKa = 4.46EE144 pKa = 4.55EE145 pKa = 5.08TMQLWNSNN153 pKa = 3.41

Molecular weight: 17.37 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A179DLQ8|A0A179DLQ8_9SPHI AI-2E family transporter OS=Pedobacter psychrophilus OX=1826909 GN=A5893_00630 PE=3 SV=1
MM1 pKa = 7.78GLRR4 pKa = 11.84KK5 pKa = 9.8FKK7 pKa = 10.46PVTPGTRR14 pKa = 11.84FRR16 pKa = 11.84VGNDD20 pKa = 2.94FTEE23 pKa = 4.0ITTNIPEE30 pKa = 4.08KK31 pKa = 10.88SLVVKK36 pKa = 10.28QGKK39 pKa = 9.42SGGRR43 pKa = 11.84NNTGKK48 pKa = 8.1MTMRR52 pKa = 11.84YY53 pKa = 9.51VGGGHH58 pKa = 5.74KK59 pKa = 9.72QLYY62 pKa = 9.7RR63 pKa = 11.84LIDD66 pKa = 3.83FKK68 pKa = 10.97RR69 pKa = 11.84DD70 pKa = 3.35KK71 pKa = 10.88KK72 pKa = 10.94DD73 pKa = 3.07IPATVATIEE82 pKa = 4.01YY83 pKa = 9.96DD84 pKa = 3.42PNRR87 pKa = 11.84TARR90 pKa = 11.84IALLHH95 pKa = 5.81YY96 pKa = 10.78ADD98 pKa = 3.89GEE100 pKa = 4.06KK101 pKa = 10.2RR102 pKa = 11.84YY103 pKa = 10.09IIAPEE108 pKa = 5.07GIQVGAKK115 pKa = 9.79VIAGDD120 pKa = 3.78TVAPEE125 pKa = 3.86VGNNISLANIPLGSIIHH142 pKa = 6.37NIEE145 pKa = 4.17LNPGRR150 pKa = 11.84GAQIARR156 pKa = 11.84SAGSYY161 pKa = 9.82AQLAARR167 pKa = 11.84DD168 pKa = 3.99GKK170 pKa = 10.84YY171 pKa = 9.07ATIKK175 pKa = 9.6MPSGEE180 pKa = 4.17TRR182 pKa = 11.84LILTTCTATIGTVSNSEE199 pKa = 4.05HH200 pKa = 6.4ANEE203 pKa = 4.29VLGKK207 pKa = 10.21AGRR210 pKa = 11.84KK211 pKa = 7.93RR212 pKa = 11.84WLGRR216 pKa = 11.84RR217 pKa = 11.84PRR219 pKa = 11.84VRR221 pKa = 11.84GVAMNPVDD229 pKa = 3.68HH230 pKa = 7.13PMGGGEE236 pKa = 4.08GRR238 pKa = 11.84SSGGHH243 pKa = 4.68PRR245 pKa = 11.84SRR247 pKa = 11.84NGLLSKK253 pKa = 10.64GFKK256 pKa = 9.8TRR258 pKa = 11.84DD259 pKa = 3.04KK260 pKa = 10.78KK261 pKa = 11.25KK262 pKa = 10.14NSNRR266 pKa = 11.84LIIARR271 pKa = 11.84RR272 pKa = 11.84KK273 pKa = 9.01KK274 pKa = 10.42

Molecular weight: 29.79 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3388

0

3388

1174662

52

3746

346.7

39.05

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.497 ± 0.039

0.746 ± 0.012

5.473 ± 0.028

5.879 ± 0.057

5.387 ± 0.037

6.476 ± 0.046

1.573 ± 0.019

8.384 ± 0.035

8.166 ± 0.044

9.46 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.087 ± 0.026

6.66 ± 0.047

3.443 ± 0.021

3.514 ± 0.023

3.18 ± 0.026

6.601 ± 0.04

5.55 ± 0.047

5.783 ± 0.031

1.055 ± 0.016

4.086 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski