Ancient caribou feces associated virus

Taxonomy: Viruses; unclassified viruses

Average proteome isoelectric point is 7.65

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A088FX63|A0A088FX63_9VIRU Cap OS=Ancient caribou feces associated virus OX=1542745 PE=4 SV=1
MM1 pKa = 7.85PPRR4 pKa = 11.84EE5 pKa = 3.93PRR7 pKa = 11.84FNGIRR12 pKa = 11.84FFLTYY17 pKa = 10.69ARR19 pKa = 11.84ADD21 pKa = 4.19AISIDD26 pKa = 3.91DD27 pKa = 4.1VADD30 pKa = 4.04HH31 pKa = 6.48LHH33 pKa = 5.75QLADD37 pKa = 3.16SWLEE41 pKa = 3.81IVQEE45 pKa = 3.87DD46 pKa = 3.92HH47 pKa = 7.01VPNEE51 pKa = 3.89EE52 pKa = 4.24HH53 pKa = 7.28PDD55 pKa = 3.53GGIHH59 pKa = 5.21YY60 pKa = 10.29HH61 pKa = 5.99VVLCFATRR69 pKa = 11.84YY70 pKa = 9.71QGALDD75 pKa = 3.71SFDD78 pKa = 3.97VLGHH82 pKa = 6.21HH83 pKa = 6.88PNWTPIRR90 pKa = 11.84NATVDD95 pKa = 3.42LTNRR99 pKa = 11.84RR100 pKa = 11.84HH101 pKa = 6.07YY102 pKa = 9.73IRR104 pKa = 11.84KK105 pKa = 9.7GIRR108 pKa = 11.84AKK110 pKa = 10.51EE111 pKa = 3.96DD112 pKa = 2.88QHH114 pKa = 5.44TVKK117 pKa = 10.39NHH119 pKa = 5.21KK120 pKa = 7.36TTACDD125 pKa = 3.89YY126 pKa = 10.54IIEE129 pKa = 5.02PDD131 pKa = 3.23TRR133 pKa = 11.84GNVPEE138 pKa = 4.33YY139 pKa = 10.49SSQAGRR145 pKa = 11.84LNWGGILEE153 pKa = 4.18QATTGEE159 pKa = 4.39EE160 pKa = 4.0FLALCRR166 pKa = 11.84LHH168 pKa = 6.81QPKK171 pKa = 10.54DD172 pKa = 3.05WILRR176 pKa = 11.84NEE178 pKa = 4.31ALTKK182 pKa = 10.47YY183 pKa = 9.82AASYY187 pKa = 8.13YY188 pKa = 10.29QEE190 pKa = 3.72ARR192 pKa = 11.84EE193 pKa = 4.29PKK195 pKa = 9.99KK196 pKa = 10.64SLPPGTLSSFLPRR209 pKa = 11.84WMNGSNKK216 pKa = 9.88SLPRR220 pKa = 11.84YY221 pKa = 8.96VYY223 pKa = 10.04SGSFTRR229 pKa = 11.84TSLDD233 pKa = 3.16VLIIDD238 pKa = 4.61SLIMLQPKK246 pKa = 9.31PDD248 pKa = 3.62RR249 pKa = 11.84PKK251 pKa = 10.19TLLLVGPTRR260 pKa = 11.84LGKK263 pKa = 7.0THH265 pKa = 6.27WAEE268 pKa = 3.69SLGRR272 pKa = 11.84HH273 pKa = 5.88SYY275 pKa = 8.41MCGMWRR281 pKa = 11.84SDD283 pKa = 3.53SFNDD287 pKa = 3.52RR288 pKa = 11.84DD289 pKa = 4.6DD290 pKa = 4.38YY291 pKa = 11.74LILDD295 pKa = 4.64DD296 pKa = 4.93FDD298 pKa = 6.19FDD300 pKa = 4.26FFHH303 pKa = 7.1GMRR306 pKa = 11.84KK307 pKa = 9.86AIWGAQPEE315 pKa = 4.58FTTVDD320 pKa = 2.84KK321 pKa = 10.33WRR323 pKa = 11.84KK324 pKa = 9.0GVAKK328 pKa = 9.77WGKK331 pKa = 8.62PLIWLCQEE339 pKa = 3.8EE340 pKa = 4.82KK341 pKa = 11.12NPFVALDD348 pKa = 3.52KK349 pKa = 11.05NGRR352 pKa = 11.84PVMAEE357 pKa = 4.07EE358 pKa = 3.51EE359 pKa = 4.4RR360 pKa = 11.84AWYY363 pKa = 9.18RR364 pKa = 11.84ANCVEE369 pKa = 4.04VHH371 pKa = 5.28VTTKK375 pKa = 10.72LYY377 pKa = 10.52PEE379 pKa = 4.34

Molecular weight: 43.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A088FX63|A0A088FX63_9VIRU Cap OS=Ancient caribou feces associated virus OX=1542745 PE=4 SV=1
MM1 pKa = 7.91AYY3 pKa = 9.89RR4 pKa = 11.84RR5 pKa = 11.84KK6 pKa = 8.1TRR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 8.51SKK12 pKa = 9.75GRR14 pKa = 11.84HH15 pKa = 5.18RR16 pKa = 11.84GSKK19 pKa = 9.85RR20 pKa = 11.84LSEE23 pKa = 4.13HH24 pKa = 4.82MVRR27 pKa = 11.84AIKK30 pKa = 10.31AISQIPVEE38 pKa = 4.53TKK40 pKa = 10.27RR41 pKa = 11.84FPLSHH46 pKa = 7.26PFATYY51 pKa = 8.69LTYY54 pKa = 10.6GAYY57 pKa = 10.24SSGSNAIIRR66 pKa = 11.84ANIFNPIPHH75 pKa = 7.61DD76 pKa = 4.19DD77 pKa = 3.25VSTAFKK83 pKa = 10.78SEE85 pKa = 3.76EE86 pKa = 4.27SFVGSSIEE94 pKa = 4.06SRR96 pKa = 11.84GLRR99 pKa = 11.84WQMNGYY105 pKa = 9.85LSSAVAKK112 pKa = 9.52PDD114 pKa = 2.61VWFRR118 pKa = 11.84FTVYY122 pKa = 10.6SEE124 pKa = 4.11NEE126 pKa = 4.04YY127 pKa = 10.73FAGSTDD133 pKa = 3.37GVGGVSPSDD142 pKa = 3.26KK143 pKa = 11.01LFDD146 pKa = 5.15PDD148 pKa = 4.85HH149 pKa = 7.09DD150 pKa = 4.12QTPTWSRR157 pKa = 11.84WNTQIAKK164 pKa = 9.58IHH166 pKa = 5.4FQRR169 pKa = 11.84KK170 pKa = 7.8FVLGSASVGQGSLSKK185 pKa = 10.6KK186 pKa = 10.23FYY188 pKa = 10.28IPLRR192 pKa = 11.84KK193 pKa = 9.63KK194 pKa = 8.68MQSADD199 pKa = 3.69EE200 pKa = 4.57EE201 pKa = 4.93GTTVAGTFGEE211 pKa = 4.36AKK213 pKa = 10.06GMQYY217 pKa = 11.09YY218 pKa = 8.56WVLEE222 pKa = 3.97TLGIGLTALGSDD234 pKa = 3.32ISAVIGTDD242 pKa = 3.65VYY244 pKa = 11.27FKK246 pKa = 10.96DD247 pKa = 3.58AA248 pKa = 4.21

Molecular weight: 27.65 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

627

248

379

313.5

35.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.018 ± 0.382

0.957 ± 0.567

6.22 ± 0.819

5.423 ± 0.585

4.625 ± 0.605

7.177 ± 1.004

3.509 ± 0.646

5.104 ± 0.321

5.901 ± 0.565

7.496 ± 1.336

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.914 ± 0.061

3.349 ± 0.551

5.423 ± 0.824

3.03 ± 0.116

7.018 ± 0.335

7.496 ± 2.249

6.061 ± 0.232

5.423 ± 0.371

2.711 ± 0.412

4.147 ± 0.171
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski