Microcystis phage MACPNOA1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 53 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A3S6HCJ5|A0A3S6HCJ5_9CAUD Uncharacterized protein OS=Microcystis phage MACPNOA1 OX=1969741 PE=4 SV=1
MM1 pKa = 7.92RR2 pKa = 11.84IALLLMLALLAGCASVPDD20 pKa = 4.61PNKK23 pKa = 10.09VDD25 pKa = 4.34PNHH28 pKa = 5.98QAYY31 pKa = 10.49LDD33 pKa = 3.61EE34 pKa = 4.5LAAIRR39 pKa = 11.84EE40 pKa = 4.39ANAQRR45 pKa = 11.84EE46 pKa = 4.32RR47 pKa = 11.84EE48 pKa = 3.98RR49 pKa = 11.84RR50 pKa = 11.84EE51 pKa = 3.55AYY53 pKa = 10.43ARR55 pKa = 11.84MAEE58 pKa = 4.05ACAGDD63 pKa = 4.09AEE65 pKa = 4.66CVRR68 pKa = 11.84DD69 pKa = 3.88VARR72 pKa = 11.84EE73 pKa = 4.04ATLGEE78 pKa = 4.1AFANSGGRR86 pKa = 11.84RR87 pKa = 11.84DD88 pKa = 4.0APAPQPYY95 pKa = 9.36VRR97 pKa = 11.84PKK99 pKa = 9.62TKK101 pKa = 10.26GEE103 pKa = 3.89RR104 pKa = 11.84VAEE107 pKa = 3.94IVANNLTAGLVPSLVQGAVAVRR129 pKa = 11.84QSDD132 pKa = 3.48NSSAVAISGNEE143 pKa = 4.12MIRR146 pKa = 11.84DD147 pKa = 3.59IATGAIGVASQGLQQLPGLAPSYY170 pKa = 9.65TADD173 pKa = 3.44GDD175 pKa = 3.99IVLGDD180 pKa = 3.64NTTVGRR186 pKa = 11.84DD187 pKa = 3.07QWGSDD192 pKa = 3.0NRR194 pKa = 11.84VGDD197 pKa = 3.75EE198 pKa = 4.45FGDD201 pKa = 3.81INTGTQVEE209 pKa = 4.5SGIAGDD215 pKa = 4.44GNRR218 pKa = 11.84QASPDD223 pKa = 3.83DD224 pKa = 3.75YY225 pKa = 11.61SEE227 pKa = 4.16RR228 pKa = 11.84TEE230 pKa = 4.23CTGPGCQGTNRR241 pKa = 11.84PIRR244 pKa = 11.84IALPPEE250 pKa = 4.09RR251 pKa = 11.84PEE253 pKa = 4.01PEE255 pKa = 4.2EE256 pKa = 4.48PSDD259 pKa = 3.59EE260 pKa = 4.17

Molecular weight:
27.42 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A3S6HDH7|A0A3S6HDH7_9CAUD Uncharacterized protein OS=Microcystis phage MACPNOA1 OX=1969741 PE=4 SV=1
MM1 pKa = 7.77RR2 pKa = 11.84ALSDD6 pKa = 3.27TASAVDD12 pKa = 3.43IEE14 pKa = 4.4AAIVHH19 pKa = 5.98HH20 pKa = 7.07LDD22 pKa = 2.92RR23 pKa = 11.84KK24 pKa = 8.96RR25 pKa = 11.84RR26 pKa = 11.84RR27 pKa = 11.84GASANTLRR35 pKa = 11.84AYY37 pKa = 10.23AVDD40 pKa = 3.89LRR42 pKa = 11.84QFAAYY47 pKa = 9.5LVSRR51 pKa = 11.84RR52 pKa = 11.84EE53 pKa = 3.9GVLAALVTHH62 pKa = 6.25RR63 pKa = 11.84HH64 pKa = 4.99IEE66 pKa = 3.95GWLDD70 pKa = 3.25TLSAQGVGARR80 pKa = 11.84SQARR84 pKa = 11.84KK85 pKa = 8.85LAVVRR90 pKa = 11.84GLFRR94 pKa = 11.84TCEE97 pKa = 3.85LEE99 pKa = 3.56GWIGHH104 pKa = 5.84DD105 pKa = 3.43PCRR108 pKa = 11.84DD109 pKa = 3.37VQVKK113 pKa = 8.19FRR115 pKa = 11.84RR116 pKa = 11.84VLRR119 pKa = 11.84TAPEE123 pKa = 4.16LPALYY128 pKa = 10.97AMVEE132 pKa = 4.63DD133 pKa = 4.46IGHH136 pKa = 4.77THH138 pKa = 6.0WRR140 pKa = 11.84DD141 pKa = 3.23LRR143 pKa = 11.84DD144 pKa = 3.38RR145 pKa = 11.84ALLRR149 pKa = 11.84LALDD153 pKa = 3.66AGLRR157 pKa = 11.84ISEE160 pKa = 4.46LLCLDD165 pKa = 3.51MAGCGPHH172 pKa = 6.39FVDD175 pKa = 4.47SKK177 pKa = 10.97RR178 pKa = 11.84LLVHH182 pKa = 6.51FGCKK186 pKa = 10.21GGDD189 pKa = 3.74SEE191 pKa = 4.97TSPINQRR198 pKa = 11.84TATIVQDD205 pKa = 4.15WIRR208 pKa = 11.84VRR210 pKa = 11.84GDD212 pKa = 2.92VAAPGEE218 pKa = 4.16TALFVSRR225 pKa = 11.84RR226 pKa = 11.84GTRR229 pKa = 11.84LTRR232 pKa = 11.84QMAHH236 pKa = 5.06VVVKK240 pKa = 10.02QRR242 pKa = 11.84GAAAGLPGLHH252 pKa = 5.64MHH254 pKa = 7.37LLRR257 pKa = 11.84HH258 pKa = 5.62RR259 pKa = 11.84RR260 pKa = 11.84IGDD263 pKa = 3.44VIEE266 pKa = 4.42RR267 pKa = 11.84CGLLAARR274 pKa = 11.84EE275 pKa = 4.17LAHH278 pKa = 6.72HH279 pKa = 6.74SSAEE283 pKa = 4.18TTGAVYY289 pKa = 10.25GRR291 pKa = 11.84HH292 pKa = 5.32VNTAAVQLLRR302 pKa = 11.84EE303 pKa = 4.15RR304 pKa = 11.84ADD306 pKa = 3.65LDD308 pKa = 3.88RR309 pKa = 11.84AGCAAA314 pKa = 4.47

Molecular weight:
34.62 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

53

0

53

12656

45

1437

238.8

25.93

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.238 ± 0.564

1.075 ± 0.219

5.476 ± 0.196

6.479 ± 0.398

2.702 ± 0.194

8.454 ± 0.293

1.723 ± 0.289

3.848 ± 0.242

2.331 ± 0.273

9.956 ± 0.299

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.794 ± 0.126

2.347 ± 0.174

4.828 ± 0.361

4.772 ± 0.407

9.079 ± 0.354

5.136 ± 0.211

5.61 ± 0.407

6.606 ± 0.381

1.557 ± 0.206

1.991 ± 0.191

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski