Microcystis phage MACPNOA1
Average proteome isoelectric point is 6.98
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 53 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3S6HCJ5|A0A3S6HCJ5_9CAUD Uncharacterized protein OS=Microcystis phage MACPNOA1 OX=1969741 PE=4 SV=1
MM1 pKa = 7.92 RR2 pKa = 11.84 IALLLMLALLAGCASVPDD20 pKa = 4.61 PNKK23 pKa = 10.09 VDD25 pKa = 4.34 PNHH28 pKa = 5.98 QAYY31 pKa = 10.49 LDD33 pKa = 3.61 EE34 pKa = 4.5 LAAIRR39 pKa = 11.84 EE40 pKa = 4.39 ANAQRR45 pKa = 11.84 EE46 pKa = 4.32 RR47 pKa = 11.84 EE48 pKa = 3.98 RR49 pKa = 11.84 RR50 pKa = 11.84 EE51 pKa = 3.55 AYY53 pKa = 10.43 ARR55 pKa = 11.84 MAEE58 pKa = 4.05 ACAGDD63 pKa = 4.09 AEE65 pKa = 4.66 CVRR68 pKa = 11.84 DD69 pKa = 3.88 VARR72 pKa = 11.84 EE73 pKa = 4.04 ATLGEE78 pKa = 4.1 AFANSGGRR86 pKa = 11.84 RR87 pKa = 11.84 DD88 pKa = 4.0 APAPQPYY95 pKa = 9.36 VRR97 pKa = 11.84 PKK99 pKa = 9.62 TKK101 pKa = 10.26 GEE103 pKa = 3.89 RR104 pKa = 11.84 VAEE107 pKa = 3.94 IVANNLTAGLVPSLVQGAVAVRR129 pKa = 11.84 QSDD132 pKa = 3.48 NSSAVAISGNEE143 pKa = 4.12 MIRR146 pKa = 11.84 DD147 pKa = 3.59 IATGAIGVASQGLQQLPGLAPSYY170 pKa = 9.65 TADD173 pKa = 3.44 GDD175 pKa = 3.99 IVLGDD180 pKa = 3.64 NTTVGRR186 pKa = 11.84 DD187 pKa = 3.07 QWGSDD192 pKa = 3.0 NRR194 pKa = 11.84 VGDD197 pKa = 3.75 EE198 pKa = 4.45 FGDD201 pKa = 3.81 INTGTQVEE209 pKa = 4.5 SGIAGDD215 pKa = 4.44 GNRR218 pKa = 11.84 QASPDD223 pKa = 3.83 DD224 pKa = 3.75 YY225 pKa = 11.61 SEE227 pKa = 4.16 RR228 pKa = 11.84 TEE230 pKa = 4.23 CTGPGCQGTNRR241 pKa = 11.84 PIRR244 pKa = 11.84 IALPPEE250 pKa = 4.09 RR251 pKa = 11.84 PEE253 pKa = 4.01 PEE255 pKa = 4.2 EE256 pKa = 4.48 PSDD259 pKa = 3.59 EE260 pKa = 4.17
Molecular weight: 27.42 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.599
IPC2_protein 4.52
IPC_protein 4.444
Toseland 4.279
ProMoST 4.52
Dawson 4.393
Bjellqvist 4.571
Wikipedia 4.266
Rodwell 4.279
Grimsley 4.19
Solomon 4.393
Lehninger 4.342
Nozaki 4.495
DTASelect 4.673
Thurlkill 4.279
EMBOSS 4.291
Sillero 4.558
Patrickios 4.012
IPC_peptide 4.393
IPC2_peptide 4.546
IPC2.peptide.svr19 4.491
Protein with the highest isoelectric point:
>tr|A0A3S6HDH7|A0A3S6HDH7_9CAUD Uncharacterized protein OS=Microcystis phage MACPNOA1 OX=1969741 PE=4 SV=1
MM1 pKa = 7.77 RR2 pKa = 11.84 ALSDD6 pKa = 3.27 TASAVDD12 pKa = 3.43 IEE14 pKa = 4.4 AAIVHH19 pKa = 5.98 HH20 pKa = 7.07 LDD22 pKa = 2.92 RR23 pKa = 11.84 KK24 pKa = 8.96 RR25 pKa = 11.84 RR26 pKa = 11.84 RR27 pKa = 11.84 GASANTLRR35 pKa = 11.84 AYY37 pKa = 10.23 AVDD40 pKa = 3.89 LRR42 pKa = 11.84 QFAAYY47 pKa = 9.5 LVSRR51 pKa = 11.84 RR52 pKa = 11.84 EE53 pKa = 3.9 GVLAALVTHH62 pKa = 6.25 RR63 pKa = 11.84 HH64 pKa = 4.99 IEE66 pKa = 3.95 GWLDD70 pKa = 3.25 TLSAQGVGARR80 pKa = 11.84 SQARR84 pKa = 11.84 KK85 pKa = 8.85 LAVVRR90 pKa = 11.84 GLFRR94 pKa = 11.84 TCEE97 pKa = 3.85 LEE99 pKa = 3.56 GWIGHH104 pKa = 5.84 DD105 pKa = 3.43 PCRR108 pKa = 11.84 DD109 pKa = 3.37 VQVKK113 pKa = 8.19 FRR115 pKa = 11.84 RR116 pKa = 11.84 VLRR119 pKa = 11.84 TAPEE123 pKa = 4.16 LPALYY128 pKa = 10.97 AMVEE132 pKa = 4.63 DD133 pKa = 4.46 IGHH136 pKa = 4.77 THH138 pKa = 6.0 WRR140 pKa = 11.84 DD141 pKa = 3.23 LRR143 pKa = 11.84 DD144 pKa = 3.38 RR145 pKa = 11.84 ALLRR149 pKa = 11.84 LALDD153 pKa = 3.66 AGLRR157 pKa = 11.84 ISEE160 pKa = 4.46 LLCLDD165 pKa = 3.51 MAGCGPHH172 pKa = 6.39 FVDD175 pKa = 4.47 SKK177 pKa = 10.97 RR178 pKa = 11.84 LLVHH182 pKa = 6.51 FGCKK186 pKa = 10.21 GGDD189 pKa = 3.74 SEE191 pKa = 4.97 TSPINQRR198 pKa = 11.84 TATIVQDD205 pKa = 4.15 WIRR208 pKa = 11.84 VRR210 pKa = 11.84 GDD212 pKa = 2.92 VAAPGEE218 pKa = 4.16 TALFVSRR225 pKa = 11.84 RR226 pKa = 11.84 GTRR229 pKa = 11.84 LTRR232 pKa = 11.84 QMAHH236 pKa = 5.06 VVVKK240 pKa = 10.02 QRR242 pKa = 11.84 GAAAGLPGLHH252 pKa = 5.64 MHH254 pKa = 7.37 LLRR257 pKa = 11.84 HH258 pKa = 5.62 RR259 pKa = 11.84 RR260 pKa = 11.84 IGDD263 pKa = 3.44 VIEE266 pKa = 4.42 RR267 pKa = 11.84 CGLLAARR274 pKa = 11.84 EE275 pKa = 4.17 LAHH278 pKa = 6.72 HH279 pKa = 6.74 SSAEE283 pKa = 4.18 TTGAVYY289 pKa = 10.25 GRR291 pKa = 11.84 HH292 pKa = 5.32 VNTAAVQLLRR302 pKa = 11.84 EE303 pKa = 4.15 RR304 pKa = 11.84 ADD306 pKa = 3.65 LDD308 pKa = 3.88 RR309 pKa = 11.84 AGCAAA314 pKa = 4.47
Molecular weight: 34.62 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.337
IPC2_protein 9.194
IPC_protein 9.911
Toseland 10.204
ProMoST 9.999
Dawson 10.365
Bjellqvist 10.16
Wikipedia 10.599
Rodwell 10.394
Grimsley 10.423
Solomon 10.482
Lehninger 10.438
Nozaki 10.321
DTASelect 10.116
Thurlkill 10.262
EMBOSS 10.628
Sillero 10.321
Patrickios 10.028
IPC_peptide 10.482
IPC2_peptide 9.56
IPC2.peptide.svr19 8.328
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
53
0
53
12656
45
1437
238.8
25.93
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
14.238 ± 0.564
1.075 ± 0.219
5.476 ± 0.196
6.479 ± 0.398
2.702 ± 0.194
8.454 ± 0.293
1.723 ± 0.289
3.848 ± 0.242
2.331 ± 0.273
9.956 ± 0.299
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.794 ± 0.126
2.347 ± 0.174
4.828 ± 0.361
4.772 ± 0.407
9.079 ± 0.354
5.136 ± 0.211
5.61 ± 0.407
6.606 ± 0.381
1.557 ± 0.206
1.991 ± 0.191
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here