Amino acid dipepetide frequency for Microcystis phage MACPNOA1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
21.177AlaAla: 21.177 ± 2.058
1.501AlaCys: 1.501 ± 0.576
8.139AlaAsp: 8.139 ± 0.924
10.747AlaGlu: 10.747 ± 0.937
3.793AlaPhe: 3.793 ± 0.578
10.352AlaGly: 10.352 ± 1.09
1.58AlaHis: 1.58 ± 0.385
6.401AlaIle: 6.401 ± 0.811
4.662AlaLys: 4.662 ± 0.933
14.935AlaLeu: 14.935 ± 1.255
3.003AlaMet: 3.003 ± 0.568
2.608AlaAsn: 2.608 ± 0.525
5.373AlaPro: 5.373 ± 0.934
7.191AlaGln: 7.191 ± 1.111
12.169AlaArg: 12.169 ± 1.116
6.559AlaSer: 6.559 ± 0.781
8.692AlaThr: 8.692 ± 0.842
8.692AlaVal: 8.692 ± 0.732
1.976AlaTrp: 1.976 ± 0.501
2.766AlaTyr: 2.766 ± 0.438
0.0AlaXaa: 0.0 ± 0.0
Cys
2.292CysAla: 2.292 ± 0.632
0.079CysCys: 0.079 ± 0.09
0.553CysAsp: 0.553 ± 0.478
0.869CysGlu: 0.869 ± 0.354
0.237CysPhe: 0.237 ± 0.141
0.711CysGly: 0.711 ± 0.287
0.158CysHis: 0.158 ± 0.117
0.395CysIle: 0.395 ± 0.184
0.158CysLys: 0.158 ± 0.117
0.79CysLeu: 0.79 ± 0.268
0.237CysMet: 0.237 ± 0.18
0.158CysAsn: 0.158 ± 0.106
0.237CysPro: 0.237 ± 0.133
0.237CysGln: 0.237 ± 0.126
1.027CysArg: 1.027 ± 0.275
1.106CysSer: 1.106 ± 0.438
0.79CysThr: 0.79 ± 0.265
0.474CysVal: 0.474 ± 0.187
0.158CysTrp: 0.158 ± 0.096
0.079CysTyr: 0.079 ± 0.078
0.0CysXaa: 0.0 ± 0.0
Asp
6.954AspAla: 6.954 ± 0.73
0.474AspCys: 0.474 ± 0.19
2.529AspAsp: 2.529 ± 0.377
3.793AspGlu: 3.793 ± 0.667
0.948AspPhe: 0.948 ± 0.28
5.531AspGly: 5.531 ± 0.832
0.948AspHis: 0.948 ± 0.31
2.292AspIle: 2.292 ± 0.424
0.79AspLys: 0.79 ± 0.275
4.741AspLeu: 4.741 ± 0.646
1.027AspMet: 1.027 ± 0.375
1.501AspAsn: 1.501 ± 0.362
4.109AspPro: 4.109 ± 0.471
1.976AspGln: 1.976 ± 0.28
4.583AspArg: 4.583 ± 0.709
2.608AspSer: 2.608 ± 0.472
3.24AspThr: 3.24 ± 0.598
4.504AspVal: 4.504 ± 0.64
1.027AspTrp: 1.027 ± 0.227
1.185AspTyr: 1.185 ± 0.408
0.0AspXaa: 0.0 ± 0.0
Glu
10.036GluAla: 10.036 ± 1.123
0.869GluCys: 0.869 ± 0.414
1.976GluAsp: 1.976 ± 0.353
3.398GluGlu: 3.398 ± 0.603
2.687GluPhe: 2.687 ± 0.464
3.872GluGly: 3.872 ± 0.547
1.185GluHis: 1.185 ± 0.314
3.003GluIle: 3.003 ± 0.441
1.027GluLys: 1.027 ± 0.397
9.008GluLeu: 9.008 ± 1.143
1.501GluMet: 1.501 ± 0.382
1.106GluAsn: 1.106 ± 0.283
2.45GluPro: 2.45 ± 0.448
3.398GluGln: 3.398 ± 0.537
8.455GluArg: 8.455 ± 0.824
3.398GluSer: 3.398 ± 0.51
2.45GluThr: 2.45 ± 0.436
3.398GluVal: 3.398 ± 0.541
0.395GluTrp: 0.395 ± 0.166
1.185GluTyr: 1.185 ± 0.303
0.0GluXaa: 0.0 ± 0.0
Phe
3.714PheAla: 3.714 ± 0.461
0.237PheCys: 0.237 ± 0.14
1.976PheAsp: 1.976 ± 0.442
1.896PheGlu: 1.896 ± 0.434
0.632PhePhe: 0.632 ± 0.213
3.319PheGly: 3.319 ± 0.517
0.316PheHis: 0.316 ± 0.184
0.79PheIle: 0.79 ± 0.253
0.711PheLys: 0.711 ± 0.244
1.343PheLeu: 1.343 ± 0.352
0.553PheMet: 0.553 ± 0.204
1.106PheAsn: 1.106 ± 0.336
0.869PhePro: 0.869 ± 0.305
0.711PheGln: 0.711 ± 0.306
1.976PheArg: 1.976 ± 0.313
1.422PheSer: 1.422 ± 0.262
1.501PheThr: 1.501 ± 0.309
3.003PheVal: 3.003 ± 0.527
0.474PheTrp: 0.474 ± 0.268
0.474PheTyr: 0.474 ± 0.151
0.0PheXaa: 0.0 ± 0.0
Gly
12.88GlyAla: 12.88 ± 1.183
1.185GlyCys: 1.185 ± 0.358
5.373GlyAsp: 5.373 ± 0.639
5.531GlyGlu: 5.531 ± 0.754
2.845GlyPhe: 2.845 ± 0.513
6.559GlyGly: 6.559 ± 0.75
1.501GlyHis: 1.501 ± 0.385
2.924GlyIle: 2.924 ± 0.533
2.608GlyLys: 2.608 ± 0.409
8.297GlyLeu: 8.297 ± 0.732
1.58GlyMet: 1.58 ± 0.358
2.134GlyAsn: 2.134 ± 0.381
3.161GlyPro: 3.161 ± 0.468
4.188GlyGln: 4.188 ± 0.646
6.401GlyArg: 6.401 ± 0.849
4.346GlySer: 4.346 ± 0.629
4.346GlyThr: 4.346 ± 0.774
5.294GlyVal: 5.294 ± 0.673
1.58GlyTrp: 1.58 ± 0.366
1.817GlyTyr: 1.817 ± 0.35
0.0GlyXaa: 0.0 ± 0.0
His
2.845HisAla: 2.845 ± 0.555
0.079HisCys: 0.079 ± 0.09
1.106HisAsp: 1.106 ± 0.387
0.869HisGlu: 0.869 ± 0.322
0.316HisPhe: 0.316 ± 0.18
2.292HisGly: 2.292 ± 0.654
0.553HisHis: 0.553 ± 0.241
0.632HisIle: 0.632 ± 0.222
0.316HisLys: 0.316 ± 0.238
1.738HisLeu: 1.738 ± 0.445
0.158HisMet: 0.158 ± 0.098
0.079HisAsn: 0.079 ± 0.073
1.027HisPro: 1.027 ± 0.292
0.553HisGln: 0.553 ± 0.221
1.343HisArg: 1.343 ± 0.352
0.869HisSer: 0.869 ± 0.298
0.632HisThr: 0.632 ± 0.255
1.185HisVal: 1.185 ± 0.325
0.316HisTrp: 0.316 ± 0.176
0.316HisTyr: 0.316 ± 0.161
0.0HisXaa: 0.0 ± 0.0
Ile
5.215IleAla: 5.215 ± 1.037
0.395IleCys: 0.395 ± 0.18
2.529IleAsp: 2.529 ± 0.639
3.872IleGlu: 3.872 ± 0.642
0.474IlePhe: 0.474 ± 0.23
4.346IleGly: 4.346 ± 0.567
0.711IleHis: 0.711 ± 0.259
1.501IleIle: 1.501 ± 0.305
1.027IleLys: 1.027 ± 0.235
2.371IleLeu: 2.371 ± 0.447
0.395IleMet: 0.395 ± 0.196
1.976IleAsn: 1.976 ± 0.384
1.106IlePro: 1.106 ± 0.316
1.343IleGln: 1.343 ± 0.368
3.161IleArg: 3.161 ± 0.482
2.45IleSer: 2.45 ± 0.544
2.608IleThr: 2.608 ± 0.534
2.292IleVal: 2.292 ± 0.478
0.158IleTrp: 0.158 ± 0.11
0.553IleTyr: 0.553 ± 0.193
0.0IleXaa: 0.0 ± 0.0
Lys
3.161LysAla: 3.161 ± 0.722
0.158LysCys: 0.158 ± 0.118
0.79LysAsp: 0.79 ± 0.243
0.711LysGlu: 0.711 ± 0.22
0.711LysPhe: 0.711 ± 0.338
1.896LysGly: 1.896 ± 0.339
0.553LysHis: 0.553 ± 0.205
0.79LysIle: 0.79 ± 0.22
0.316LysLys: 0.316 ± 0.152
2.45LysLeu: 2.45 ± 0.526
0.474LysMet: 0.474 ± 0.166
0.948LysAsn: 0.948 ± 0.312
2.055LysPro: 2.055 ± 0.506
0.632LysGln: 0.632 ± 0.225
2.529LysArg: 2.529 ± 0.468
1.343LysSer: 1.343 ± 0.308
1.501LysThr: 1.501 ± 0.374
1.185LysVal: 1.185 ± 0.424
0.79LysTrp: 0.79 ± 0.326
0.316LysTyr: 0.316 ± 0.17
0.0LysXaa: 0.0 ± 0.0
Leu
12.248LeuAla: 12.248 ± 1.329
1.106LeuCys: 1.106 ± 0.348
7.349LeuAsp: 7.349 ± 0.733
5.531LeuGlu: 5.531 ± 0.856
2.292LeuPhe: 2.292 ± 0.38
8.139LeuGly: 8.139 ± 0.806
1.58LeuHis: 1.58 ± 0.406
2.45LeuIle: 2.45 ± 0.635
2.45LeuLys: 2.45 ± 0.441
10.115LeuLeu: 10.115 ± 0.766
1.976LeuMet: 1.976 ± 0.366
1.976LeuAsn: 1.976 ± 0.472
4.504LeuPro: 4.504 ± 0.644
7.27LeuGln: 7.27 ± 0.882
11.379LeuArg: 11.379 ± 0.938
4.425LeuSer: 4.425 ± 0.635
6.243LeuThr: 6.243 ± 0.854
5.294LeuVal: 5.294 ± 0.473
1.106LeuTrp: 1.106 ± 0.304
2.134LeuTyr: 2.134 ± 0.384
0.0LeuXaa: 0.0 ± 0.0
Met
3.24MetAla: 3.24 ± 0.519
0.079MetCys: 0.079 ± 0.075
1.264MetAsp: 1.264 ± 0.283
0.553MetGlu: 0.553 ± 0.179
0.158MetPhe: 0.158 ± 0.125
1.185MetGly: 1.185 ± 0.283
0.395MetHis: 0.395 ± 0.155
0.711MetIle: 0.711 ± 0.203
0.237MetLys: 0.237 ± 0.162
2.134MetLeu: 2.134 ± 0.418
0.079MetMet: 0.079 ± 0.092
0.474MetAsn: 0.474 ± 0.192
1.027MetPro: 1.027 ± 0.203
0.79MetGln: 0.79 ± 0.253
2.371MetArg: 2.371 ± 0.44
1.501MetSer: 1.501 ± 0.347
0.869MetThr: 0.869 ± 0.265
0.79MetVal: 0.79 ± 0.229
0.079MetTrp: 0.079 ± 0.085
0.0MetTyr: 0.0 ± 0.0
0.0MetXaa: 0.0 ± 0.0
Asn
3.635AsnAla: 3.635 ± 0.604
0.237AsnCys: 0.237 ± 0.108
0.869AsnAsp: 0.869 ± 0.266
0.474AsnGlu: 0.474 ± 0.195
0.632AsnPhe: 0.632 ± 0.181
1.896AsnGly: 1.896 ± 0.445
0.395AsnHis: 0.395 ± 0.187
0.79AsnIle: 0.79 ± 0.253
0.316AsnLys: 0.316 ± 0.156
2.687AsnLeu: 2.687 ± 0.453
0.237AsnMet: 0.237 ± 0.143
0.474AsnAsn: 0.474 ± 0.188
2.292AsnPro: 2.292 ± 0.359
1.264AsnGln: 1.264 ± 0.341
2.45AsnArg: 2.45 ± 0.686
1.264AsnSer: 1.264 ± 0.434
1.58AsnThr: 1.58 ± 0.372
1.422AsnVal: 1.422 ± 0.272
0.237AsnTrp: 0.237 ± 0.124
0.316AsnTyr: 0.316 ± 0.177
0.0AsnXaa: 0.0 ± 0.0
Pro
6.401ProAla: 6.401 ± 1.006
0.79ProCys: 0.79 ± 0.279
2.845ProAsp: 2.845 ± 0.528
4.03ProGlu: 4.03 ± 0.686
1.027ProPhe: 1.027 ± 0.303
4.662ProGly: 4.662 ± 0.77
1.027ProHis: 1.027 ± 0.287
1.817ProIle: 1.817 ± 0.401
1.264ProLys: 1.264 ± 0.344
3.714ProLeu: 3.714 ± 0.578
0.869ProMet: 0.869 ± 0.249
1.343ProAsn: 1.343 ± 0.31
2.213ProPro: 2.213 ± 0.604
1.976ProGln: 1.976 ± 0.374
3.161ProArg: 3.161 ± 0.633
2.45ProSer: 2.45 ± 0.428
2.529ProThr: 2.529 ± 0.594
3.635ProVal: 3.635 ± 0.633
1.106ProTrp: 1.106 ± 0.33
1.422ProTyr: 1.422 ± 0.358
0.0ProXaa: 0.0 ± 0.0
Gln
6.717GlnAla: 6.717 ± 1.163
0.316GlnCys: 0.316 ± 0.149
1.738GlnAsp: 1.738 ± 0.452
1.659GlnGlu: 1.659 ± 0.449
2.055GlnPhe: 2.055 ± 0.51
3.951GlnGly: 3.951 ± 0.556
0.869GlnHis: 0.869 ± 0.327
2.529GlnIle: 2.529 ± 0.446
0.474GlnLys: 0.474 ± 0.171
5.847GlnLeu: 5.847 ± 0.678
1.185GlnMet: 1.185 ± 0.389
0.869GlnAsn: 0.869 ± 0.317
2.924GlnPro: 2.924 ± 0.638
3.161GlnGln: 3.161 ± 0.727
4.662GlnArg: 4.662 ± 0.592
1.817GlnSer: 1.817 ± 0.288
1.738GlnThr: 1.738 ± 0.315
3.398GlnVal: 3.398 ± 0.436
1.106GlnTrp: 1.106 ± 0.274
0.711GlnTyr: 0.711 ± 0.224
0.0GlnXaa: 0.0 ± 0.0
Arg
12.959ArgAla: 12.959 ± 1.317
0.869ArgCys: 0.869 ± 0.302
5.768ArgAsp: 5.768 ± 0.721
7.349ArgGlu: 7.349 ± 0.734
2.292ArgPhe: 2.292 ± 0.495
6.875ArgGly: 6.875 ± 0.97
1.264ArgHis: 1.264 ± 0.366
3.872ArgIle: 3.872 ± 0.396
2.371ArgLys: 2.371 ± 0.548
7.428ArgLeu: 7.428 ± 0.758
1.422ArgMet: 1.422 ± 0.305
1.58ArgAsn: 1.58 ± 0.28
3.951ArgPro: 3.951 ± 0.576
3.793ArgGln: 3.793 ± 0.587
7.902ArgArg: 7.902 ± 1.252
4.109ArgSer: 4.109 ± 0.639
5.373ArgThr: 5.373 ± 0.68
7.665ArgVal: 7.665 ± 0.675
1.817ArgTrp: 1.817 ± 0.415
2.134ArgTyr: 2.134 ± 0.542
0.0ArgXaa: 0.0 ± 0.0
Ser
7.27SerAla: 7.27 ± 0.741
0.869SerCys: 0.869 ± 0.259
2.687SerAsp: 2.687 ± 0.436
3.951SerGlu: 3.951 ± 0.499
1.343SerPhe: 1.343 ± 0.42
5.373SerGly: 5.373 ± 0.568
1.106SerHis: 1.106 ± 0.306
2.213SerIle: 2.213 ± 0.337
1.264SerLys: 1.264 ± 0.396
4.425SerLeu: 4.425 ± 0.49
0.711SerMet: 0.711 ± 0.245
1.422SerAsn: 1.422 ± 0.467
2.608SerPro: 2.608 ± 0.517
1.896SerGln: 1.896 ± 0.371
3.477SerArg: 3.477 ± 0.597
1.896SerSer: 1.896 ± 0.523
3.872SerThr: 3.872 ± 0.741
3.951SerVal: 3.951 ± 0.661
0.316SerTrp: 0.316 ± 0.135
0.711SerTyr: 0.711 ± 0.206
0.0SerXaa: 0.0 ± 0.0
Thr
7.665ThrAla: 7.665 ± 0.962
0.632ThrCys: 0.632 ± 0.223
2.687ThrAsp: 2.687 ± 0.502
2.924ThrGlu: 2.924 ± 0.556
2.134ThrPhe: 2.134 ± 0.474
6.164ThrGly: 6.164 ± 1.081
1.185ThrHis: 1.185 ± 0.31
2.134ThrIle: 2.134 ± 0.456
1.027ThrLys: 1.027 ± 0.274
7.033ThrLeu: 7.033 ± 0.874
0.474ThrMet: 0.474 ± 0.197
0.948ThrAsn: 0.948 ± 0.299
3.951ThrPro: 3.951 ± 0.61
1.896ThrGln: 1.896 ± 0.372
3.24ThrArg: 3.24 ± 0.487
3.003ThrSer: 3.003 ± 0.53
3.556ThrThr: 3.556 ± 0.695
3.24ThrVal: 3.24 ± 0.723
1.027ThrTrp: 1.027 ± 0.281
1.185ThrTyr: 1.185 ± 0.283
0.0ThrXaa: 0.0 ± 0.0
Val
8.692ValAla: 8.692 ± 0.986
0.553ValCys: 0.553 ± 0.239
3.635ValAsp: 3.635 ± 0.581
4.504ValGlu: 4.504 ± 0.469
1.976ValPhe: 1.976 ± 0.494
4.662ValGly: 4.662 ± 0.573
1.501ValHis: 1.501 ± 0.469
2.687ValIle: 2.687 ± 0.445
1.659ValLys: 1.659 ± 0.332
6.243ValLeu: 6.243 ± 0.657
1.264ValMet: 1.264 ± 0.412
1.896ValAsn: 1.896 ± 0.329
2.687ValPro: 2.687 ± 0.445
4.267ValGln: 4.267 ± 0.707
6.006ValArg: 6.006 ± 0.78
4.267ValSer: 4.267 ± 0.44
2.766ValThr: 2.766 ± 0.617
3.714ValVal: 3.714 ± 0.724
1.027ValTrp: 1.027 ± 0.31
2.055ValTyr: 2.055 ± 0.581
0.0ValXaa: 0.0 ± 0.0
Trp
2.134TrpAla: 2.134 ± 0.426
0.158TrpCys: 0.158 ± 0.101
0.158TrpAsp: 0.158 ± 0.1
0.632TrpGlu: 0.632 ± 0.194
0.316TrpPhe: 0.316 ± 0.175
1.343TrpGly: 1.343 ± 0.345
0.395TrpHis: 0.395 ± 0.198
0.237TrpIle: 0.237 ± 0.174
0.395TrpLys: 0.395 ± 0.174
1.976TrpLeu: 1.976 ± 0.384
0.474TrpMet: 0.474 ± 0.177
0.237TrpAsn: 0.237 ± 0.152
0.948TrpPro: 0.948 ± 0.275
0.79TrpGln: 0.79 ± 0.296
1.817TrpArg: 1.817 ± 0.447
1.106TrpSer: 1.106 ± 0.351
0.632TrpThr: 0.632 ± 0.208
1.106TrpVal: 1.106 ± 0.254
0.474TrpTrp: 0.474 ± 0.17
0.237TrpTyr: 0.237 ± 0.126
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.161TyrAla: 3.161 ± 0.333
0.158TyrCys: 0.158 ± 0.112
0.79TyrAsp: 0.79 ± 0.218
1.501TyrGlu: 1.501 ± 0.384
0.158TyrPhe: 0.158 ± 0.114
1.422TyrGly: 1.422 ± 0.448
0.0TyrHis: 0.0 ± 0.0
0.316TyrIle: 0.316 ± 0.152
0.237TyrLys: 0.237 ± 0.127
2.292TyrLeu: 2.292 ± 0.57
0.237TyrMet: 0.237 ± 0.13
0.632TyrAsn: 0.632 ± 0.237
0.79TyrPro: 0.79 ± 0.255
0.395TyrGln: 0.395 ± 0.135
2.687TyrArg: 2.687 ± 0.547
1.422TyrSer: 1.422 ± 0.284
1.185TyrThr: 1.185 ± 0.462
1.817TyrVal: 1.817 ± 0.421
0.395TyrTrp: 0.395 ± 0.185
0.316TyrTyr: 0.316 ± 0.135
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 53 proteins (12656 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski