Serratia phage Slocum

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Demerecviridae; unclassified Demerecviridae

Average proteome isoelectric point is 6.35

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 168 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5P8PLR4|A0A5P8PLR4_9CAUD Uncharacterized protein OS=Serratia phage Slocum OX=2601679 GN=CPT_Slocum_019 PE=4 SV=1
MM1 pKa = 7.1MEE3 pKa = 4.46SVKK6 pKa = 9.54NTYY9 pKa = 10.6LEE11 pKa = 5.13GIATSFEE18 pKa = 4.85GIDD21 pKa = 4.43GDD23 pKa = 4.46YY24 pKa = 11.31DD25 pKa = 3.09WTTYY29 pKa = 11.28YY30 pKa = 10.55DD31 pKa = 3.8VKK33 pKa = 10.24LTPDD37 pKa = 3.6FRR39 pKa = 11.84ALVGINLLEE48 pKa = 4.85KK49 pKa = 10.61GLNLQDD55 pKa = 3.55YY56 pKa = 9.52PEE58 pKa = 5.24ANFSISTNSITPLASLEE75 pKa = 4.26VYY77 pKa = 10.75ADD79 pKa = 3.85EE80 pKa = 5.45EE81 pKa = 4.33QMQPLATIEE90 pKa = 4.36FDD92 pKa = 3.38TVMTFIFKK100 pKa = 10.54

Molecular weight: 11.35 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5P8PLJ2|A0A5P8PLJ2_9CAUD Uncharacterized protein OS=Serratia phage Slocum OX=2601679 GN=CPT_Slocum_032 PE=4 SV=1
MM1 pKa = 7.88QIRR4 pKa = 11.84QCLLEE9 pKa = 4.49NGLYY13 pKa = 9.64VQIRR17 pKa = 11.84HH18 pKa = 5.51IRR20 pKa = 11.84RR21 pKa = 11.84KK22 pKa = 9.58YY23 pKa = 8.38RR24 pKa = 11.84AAGAFRR30 pKa = 11.84GKK32 pKa = 10.64FEE34 pKa = 5.07IIGGKK39 pKa = 9.08LRR41 pKa = 11.84SYY43 pKa = 11.0QNNPYY48 pKa = 10.23FGVVLL53 pKa = 4.09

Molecular weight: 6.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

168

0

168

32733

34

1690

194.8

21.87

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.019 ± 0.321

1.075 ± 0.095

6.055 ± 0.164

6.651 ± 0.269

4.085 ± 0.144

6.669 ± 0.284

2.01 ± 0.144

6.122 ± 0.147

6.452 ± 0.27

8.197 ± 0.28

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.018 ± 0.108

4.943 ± 0.231

3.657 ± 0.168

3.559 ± 0.147

4.983 ± 0.149

6.244 ± 0.171

6.104 ± 0.332

7.014 ± 0.149

1.332 ± 0.129

3.81 ± 0.129
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski