Amino acid dipepetide frequency for Serratia phage Slocum

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.393AlaAla: 7.393 ± 0.821
0.703AlaCys: 0.703 ± 0.155
4.369AlaAsp: 4.369 ± 0.344
5.285AlaGlu: 5.285 ± 0.508
2.78AlaPhe: 2.78 ± 0.275
5.408AlaGly: 5.408 ± 0.631
1.558AlaHis: 1.558 ± 0.238
5.377AlaIle: 5.377 ± 0.474
6.141AlaLys: 6.141 ± 0.557
7.149AlaLeu: 7.149 ± 0.483
2.505AlaMet: 2.505 ± 0.31
4.094AlaAsn: 4.094 ± 0.429
2.566AlaPro: 2.566 ± 0.345
3.086AlaGln: 3.086 ± 0.405
3.849AlaArg: 3.849 ± 0.336
4.155AlaSer: 4.155 ± 0.36
4.919AlaThr: 4.919 ± 0.553
5.346AlaVal: 5.346 ± 0.358
1.191AlaTrp: 1.191 ± 0.234
2.322AlaTyr: 2.322 ± 0.232
0.0AlaXaa: 0.0 ± 0.0
Cys
0.855CysAla: 0.855 ± 0.187
0.183CysCys: 0.183 ± 0.09
0.642CysAsp: 0.642 ± 0.16
0.458CysGlu: 0.458 ± 0.127
0.458CysPhe: 0.458 ± 0.103
0.855CysGly: 0.855 ± 0.18
0.244CysHis: 0.244 ± 0.086
0.458CysIle: 0.458 ± 0.127
1.069CysLys: 1.069 ± 0.203
1.039CysLeu: 1.039 ± 0.171
0.275CysMet: 0.275 ± 0.091
0.489CysAsn: 0.489 ± 0.126
0.336CysPro: 0.336 ± 0.103
0.55CysGln: 0.55 ± 0.153
0.58CysArg: 0.58 ± 0.124
0.428CysSer: 0.428 ± 0.097
0.764CysThr: 0.764 ± 0.141
0.611CysVal: 0.611 ± 0.13
0.153CysTrp: 0.153 ± 0.068
0.306CysTyr: 0.306 ± 0.091
0.0CysXaa: 0.0 ± 0.0
Asp
5.408AspAla: 5.408 ± 0.387
0.611AspCys: 0.611 ± 0.146
2.689AspAsp: 2.689 ± 0.3
3.88AspGlu: 3.88 ± 0.351
3.055AspPhe: 3.055 ± 0.267
4.735AspGly: 4.735 ± 0.864
0.886AspHis: 0.886 ± 0.167
3.819AspIle: 3.819 ± 0.281
3.88AspLys: 3.88 ± 0.415
5.133AspLeu: 5.133 ± 0.428
2.077AspMet: 2.077 ± 0.219
2.291AspAsn: 2.291 ± 0.285
2.566AspPro: 2.566 ± 0.244
1.741AspGln: 1.741 ± 0.179
2.505AspArg: 2.505 ± 0.3
3.422AspSer: 3.422 ± 0.35
3.911AspThr: 3.911 ± 0.354
4.338AspVal: 4.338 ± 0.342
0.917AspTrp: 0.917 ± 0.194
2.689AspTyr: 2.689 ± 0.312
0.0AspXaa: 0.0 ± 0.0
Glu
5.133GluAla: 5.133 ± 0.466
0.794GluCys: 0.794 ± 0.177
3.88GluAsp: 3.88 ± 0.444
5.041GluGlu: 5.041 ± 0.526
2.597GluPhe: 2.597 ± 0.268
3.849GluGly: 3.849 ± 0.365
1.344GluHis: 1.344 ± 0.236
4.155GluIle: 4.155 ± 0.432
4.338GluLys: 4.338 ± 0.397
7.118GluLeu: 7.118 ± 0.523
2.322GluMet: 2.322 ± 0.344
2.597GluAsn: 2.597 ± 0.299
1.803GluPro: 1.803 ± 0.282
2.78GluGln: 2.78 ± 0.364
3.147GluArg: 3.147 ± 0.419
3.391GluSer: 3.391 ± 0.315
3.33GluThr: 3.33 ± 0.313
4.98GluVal: 4.98 ± 0.431
1.13GluTrp: 1.13 ± 0.177
2.78GluTyr: 2.78 ± 0.375
0.0GluXaa: 0.0 ± 0.0
Phe
2.811PheAla: 2.811 ± 0.284
0.489PheCys: 0.489 ± 0.158
2.414PheAsp: 2.414 ± 0.318
2.78PheGlu: 2.78 ± 0.282
1.65PhePhe: 1.65 ± 0.237
2.719PheGly: 2.719 ± 0.296
1.008PheHis: 1.008 ± 0.236
2.383PheIle: 2.383 ± 0.301
2.811PheLys: 2.811 ± 0.289
2.75PheLeu: 2.75 ± 0.337
1.375PheMet: 1.375 ± 0.234
2.322PheAsn: 2.322 ± 0.272
1.619PhePro: 1.619 ± 0.208
1.283PheGln: 1.283 ± 0.187
2.047PheArg: 2.047 ± 0.284
2.872PheSer: 2.872 ± 0.333
2.933PheThr: 2.933 ± 0.372
2.291PheVal: 2.291 ± 0.263
0.672PheTrp: 0.672 ± 0.183
1.619PheTyr: 1.619 ± 0.213
0.0PheXaa: 0.0 ± 0.0
Gly
5.102GlyAla: 5.102 ± 0.523
0.855GlyCys: 0.855 ± 0.181
4.002GlyAsp: 4.002 ± 0.38
4.277GlyGlu: 4.277 ± 0.397
2.811GlyPhe: 2.811 ± 0.267
3.758GlyGly: 3.758 ± 0.421
1.191GlyHis: 1.191 ± 0.194
4.124GlyIle: 4.124 ± 0.336
4.644GlyLys: 4.644 ± 0.34
5.071GlyLeu: 5.071 ± 0.456
1.864GlyMet: 1.864 ± 0.249
3.422GlyAsn: 3.422 ± 0.504
1.253GlyPro: 1.253 ± 0.258
2.841GlyGln: 2.841 ± 0.323
3.513GlyArg: 3.513 ± 0.259
4.797GlySer: 4.797 ± 0.321
4.247GlyThr: 4.247 ± 0.764
5.133GlyVal: 5.133 ± 0.39
1.253GlyTrp: 1.253 ± 0.266
2.536GlyTyr: 2.536 ± 0.254
0.0GlyXaa: 0.0 ± 0.0
His
1.283HisAla: 1.283 ± 0.224
0.336HisCys: 0.336 ± 0.104
1.344HisAsp: 1.344 ± 0.226
1.253HisGlu: 1.253 ± 0.222
0.886HisPhe: 0.886 ± 0.207
1.772HisGly: 1.772 ± 0.238
0.458HisHis: 0.458 ± 0.13
1.253HisIle: 1.253 ± 0.211
1.161HisLys: 1.161 ± 0.232
1.619HisLeu: 1.619 ± 0.26
0.458HisMet: 0.458 ± 0.145
0.764HisAsn: 0.764 ± 0.151
1.008HisPro: 1.008 ± 0.184
0.58HisGln: 0.58 ± 0.129
1.008HisArg: 1.008 ± 0.176
1.314HisSer: 1.314 ± 0.202
1.039HisThr: 1.039 ± 0.175
1.528HisVal: 1.528 ± 0.257
0.183HisTrp: 0.183 ± 0.075
0.855HisTyr: 0.855 ± 0.163
0.0HisXaa: 0.0 ± 0.0
Ile
4.827IleAla: 4.827 ± 0.429
0.611IleCys: 0.611 ± 0.152
4.094IleAsp: 4.094 ± 0.323
4.43IleGlu: 4.43 ± 0.403
2.169IlePhe: 2.169 ± 0.239
3.727IleGly: 3.727 ± 0.334
1.558IleHis: 1.558 ± 0.219
3.269IleIle: 3.269 ± 0.362
3.941IleLys: 3.941 ± 0.363
4.583IleLeu: 4.583 ± 0.417
1.68IleMet: 1.68 ± 0.229
3.208IleAsn: 3.208 ± 0.278
2.719IlePro: 2.719 ± 0.281
2.016IleGln: 2.016 ± 0.267
3.758IleArg: 3.758 ± 0.312
4.43IleSer: 4.43 ± 0.366
3.391IleThr: 3.391 ± 0.344
3.972IleVal: 3.972 ± 0.289
0.703IleTrp: 0.703 ± 0.158
2.139IleTyr: 2.139 ± 0.277
0.0IleXaa: 0.0 ± 0.0
Lys
5.499LysAla: 5.499 ± 0.585
0.58LysCys: 0.58 ± 0.145
4.124LysAsp: 4.124 ± 0.468
4.124LysGlu: 4.124 ± 0.448
2.566LysPhe: 2.566 ± 0.326
3.941LysGly: 3.941 ± 0.328
1.314LysHis: 1.314 ± 0.202
3.116LysIle: 3.116 ± 0.291
4.002LysLys: 4.002 ± 0.445
6.507LysLeu: 6.507 ± 0.528
2.2LysMet: 2.2 ± 0.252
2.78LysAsn: 2.78 ± 0.325
2.475LysPro: 2.475 ± 0.309
2.627LysGln: 2.627 ± 0.283
3.116LysArg: 3.116 ± 0.334
3.3LysSer: 3.3 ± 0.309
3.788LysThr: 3.788 ± 0.27
4.399LysVal: 4.399 ± 0.414
1.008LysTrp: 1.008 ± 0.177
3.055LysTyr: 3.055 ± 0.396
0.0LysXaa: 0.0 ± 0.0
Leu
5.927LeuAla: 5.927 ± 0.446
0.886LeuCys: 0.886 ± 0.186
5.835LeuAsp: 5.835 ± 0.436
6.935LeuGlu: 6.935 ± 0.525
2.994LeuPhe: 2.994 ± 0.317
4.827LeuGly: 4.827 ± 0.373
1.497LeuHis: 1.497 ± 0.23
4.491LeuIle: 4.491 ± 0.401
4.827LeuLys: 4.827 ± 0.366
6.232LeuLeu: 6.232 ± 0.432
2.322LeuMet: 2.322 ± 0.29
5.071LeuAsn: 5.071 ± 0.485
3.208LeuPro: 3.208 ± 0.359
2.902LeuGln: 2.902 ± 0.382
4.124LeuArg: 4.124 ± 0.375
5.56LeuSer: 5.56 ± 0.432
5.163LeuThr: 5.163 ± 0.537
5.957LeuVal: 5.957 ± 0.438
0.642LeuTrp: 0.642 ± 0.131
2.566LeuTyr: 2.566 ± 0.26
0.0LeuXaa: 0.0 ± 0.0
Met
2.597MetAla: 2.597 ± 0.372
0.214MetCys: 0.214 ± 0.079
1.833MetAsp: 1.833 ± 0.264
2.077MetGlu: 2.077 ± 0.265
1.528MetPhe: 1.528 ± 0.2
1.833MetGly: 1.833 ± 0.237
0.672MetHis: 0.672 ± 0.159
1.864MetIle: 1.864 ± 0.267
2.23MetLys: 2.23 ± 0.309
2.475MetLeu: 2.475 ± 0.343
0.611MetMet: 0.611 ± 0.151
1.283MetAsn: 1.283 ± 0.196
1.13MetPro: 1.13 ± 0.19
1.314MetGln: 1.314 ± 0.214
1.558MetArg: 1.558 ± 0.24
2.2MetSer: 2.2 ± 0.284
1.864MetThr: 1.864 ± 0.239
1.558MetVal: 1.558 ± 0.192
0.214MetTrp: 0.214 ± 0.07
1.13MetTyr: 1.13 ± 0.183
0.0MetXaa: 0.0 ± 0.0
Asn
4.002AsnAla: 4.002 ± 0.496
0.489AsnCys: 0.489 ± 0.134
2.902AsnAsp: 2.902 ± 0.31
2.566AsnGlu: 2.566 ± 0.31
2.139AsnPhe: 2.139 ± 0.307
4.552AsnGly: 4.552 ± 0.429
0.764AsnHis: 0.764 ± 0.157
3.544AsnIle: 3.544 ± 0.259
3.147AsnLys: 3.147 ± 0.373
3.452AsnLeu: 3.452 ± 0.309
1.466AsnMet: 1.466 ± 0.179
2.597AsnAsn: 2.597 ± 0.356
2.23AsnPro: 2.23 ± 0.304
1.405AsnGln: 1.405 ± 0.23
2.627AsnArg: 2.627 ± 0.241
3.238AsnSer: 3.238 ± 0.37
2.841AsnThr: 2.841 ± 0.457
3.116AsnVal: 3.116 ± 0.297
0.55AsnTrp: 0.55 ± 0.153
1.803AsnTyr: 1.803 ± 0.27
0.0AsnXaa: 0.0 ± 0.0
Pro
3.3ProAla: 3.3 ± 0.487
0.428ProCys: 0.428 ± 0.122
2.139ProAsp: 2.139 ± 0.275
2.689ProGlu: 2.689 ± 0.356
1.314ProPhe: 1.314 ± 0.225
2.291ProGly: 2.291 ± 0.256
0.855ProHis: 0.855 ± 0.14
1.833ProIle: 1.833 ± 0.265
1.803ProLys: 1.803 ± 0.266
2.75ProLeu: 2.75 ± 0.298
0.794ProMet: 0.794 ± 0.148
2.352ProAsn: 2.352 ± 0.282
1.833ProPro: 1.833 ± 0.377
1.405ProGln: 1.405 ± 0.181
1.436ProArg: 1.436 ± 0.218
2.139ProSer: 2.139 ± 0.26
2.505ProThr: 2.505 ± 0.399
2.719ProVal: 2.719 ± 0.278
0.672ProTrp: 0.672 ± 0.163
1.314ProTyr: 1.314 ± 0.208
0.0ProXaa: 0.0 ± 0.0
Gln
2.872GlnAla: 2.872 ± 0.361
0.306GlnCys: 0.306 ± 0.08
2.047GlnAsp: 2.047 ± 0.197
2.536GlnGlu: 2.536 ± 0.309
1.466GlnPhe: 1.466 ± 0.19
2.139GlnGly: 2.139 ± 0.261
0.764GlnHis: 0.764 ± 0.174
2.169GlnIle: 2.169 ± 0.281
1.986GlnLys: 1.986 ± 0.336
2.963GlnLeu: 2.963 ± 0.261
1.1GlnMet: 1.1 ± 0.16
1.528GlnAsn: 1.528 ± 0.211
0.825GlnPro: 0.825 ± 0.162
1.68GlnGln: 1.68 ± 0.237
1.955GlnArg: 1.955 ± 0.267
2.077GlnSer: 2.077 ± 0.27
2.23GlnThr: 2.23 ± 0.326
2.78GlnVal: 2.78 ± 0.273
0.611GlnTrp: 0.611 ± 0.141
1.558GlnTyr: 1.558 ± 0.172
0.0GlnXaa: 0.0 ± 0.0
Arg
4.124ArgAla: 4.124 ± 0.444
0.458ArgCys: 0.458 ± 0.134
3.116ArgAsp: 3.116 ± 0.328
3.483ArgGlu: 3.483 ± 0.378
2.077ArgPhe: 2.077 ± 0.256
3.361ArgGly: 3.361 ± 0.316
0.764ArgHis: 0.764 ± 0.177
3.391ArgIle: 3.391 ± 0.308
3.361ArgLys: 3.361 ± 0.32
4.46ArgLeu: 4.46 ± 0.371
1.619ArgMet: 1.619 ± 0.265
2.78ArgAsn: 2.78 ± 0.299
1.589ArgPro: 1.589 ± 0.283
1.619ArgGln: 1.619 ± 0.256
2.505ArgArg: 2.505 ± 0.337
2.536ArgSer: 2.536 ± 0.281
2.383ArgThr: 2.383 ± 0.23
3.697ArgVal: 3.697 ± 0.339
0.336ArgTrp: 0.336 ± 0.125
2.169ArgTyr: 2.169 ± 0.305
0.0ArgXaa: 0.0 ± 0.0
Ser
5.071SerAla: 5.071 ± 0.523
0.825SerCys: 0.825 ± 0.192
3.422SerAsp: 3.422 ± 0.333
3.177SerGlu: 3.177 ± 0.291
3.391SerPhe: 3.391 ± 0.298
4.705SerGly: 4.705 ± 0.386
1.069SerHis: 1.069 ± 0.232
3.941SerIle: 3.941 ± 0.359
4.155SerLys: 4.155 ± 0.407
4.674SerLeu: 4.674 ± 0.377
1.65SerMet: 1.65 ± 0.231
2.902SerAsn: 2.902 ± 0.28
1.833SerPro: 1.833 ± 0.276
1.986SerGln: 1.986 ± 0.246
3.605SerArg: 3.605 ± 0.29
3.941SerSer: 3.941 ± 0.489
3.941SerThr: 3.941 ± 0.388
4.735SerVal: 4.735 ± 0.462
1.161SerTrp: 1.161 ± 0.175
2.261SerTyr: 2.261 ± 0.237
0.0SerXaa: 0.0 ± 0.0
Thr
4.858ThrAla: 4.858 ± 0.57
0.519ThrCys: 0.519 ± 0.118
3.177ThrAsp: 3.177 ± 0.376
3.025ThrGlu: 3.025 ± 0.313
2.352ThrPhe: 2.352 ± 0.266
5.377ThrGly: 5.377 ± 0.76
1.375ThrHis: 1.375 ± 0.192
4.277ThrIle: 4.277 ± 0.436
4.063ThrLys: 4.063 ± 0.386
4.705ThrLeu: 4.705 ± 0.4
1.68ThrMet: 1.68 ± 0.223
3.116ThrAsn: 3.116 ± 0.479
3.147ThrPro: 3.147 ± 0.431
1.833ThrGln: 1.833 ± 0.237
2.23ThrArg: 2.23 ± 0.254
3.88ThrSer: 3.88 ± 0.482
3.666ThrThr: 3.666 ± 0.438
4.674ThrVal: 4.674 ± 0.478
0.733ThrTrp: 0.733 ± 0.19
2.322ThrTyr: 2.322 ± 0.251
0.0ThrXaa: 0.0 ± 0.0
Val
5.744ValAla: 5.744 ± 0.467
0.794ValCys: 0.794 ± 0.162
4.827ValAsp: 4.827 ± 0.389
4.522ValGlu: 4.522 ± 0.396
2.627ValPhe: 2.627 ± 0.271
4.277ValGly: 4.277 ± 0.416
1.466ValHis: 1.466 ± 0.257
4.399ValIle: 4.399 ± 0.33
3.727ValLys: 3.727 ± 0.338
5.591ValLeu: 5.591 ± 0.416
2.352ValMet: 2.352 ± 0.213
2.994ValAsn: 2.994 ± 0.279
2.627ValPro: 2.627 ± 0.302
2.414ValGln: 2.414 ± 0.272
3.483ValArg: 3.483 ± 0.389
5.01ValSer: 5.01 ± 0.352
4.949ValThr: 4.949 ± 0.495
5.285ValVal: 5.285 ± 0.465
0.611ValTrp: 0.611 ± 0.152
2.444ValTyr: 2.444 ± 0.265
0.0ValXaa: 0.0 ± 0.0
Trp
0.855TrpAla: 0.855 ± 0.174
0.092TrpCys: 0.092 ± 0.045
1.161TrpAsp: 1.161 ± 0.194
1.1TrpGlu: 1.1 ± 0.194
0.458TrpPhe: 0.458 ± 0.104
0.55TrpGly: 0.55 ± 0.131
0.397TrpHis: 0.397 ± 0.164
0.642TrpIle: 0.642 ± 0.128
0.458TrpLys: 0.458 ± 0.105
1.008TrpLeu: 1.008 ± 0.18
0.55TrpMet: 0.55 ± 0.199
0.886TrpAsn: 0.886 ± 0.164
0.489TrpPro: 0.489 ± 0.12
0.244TrpGln: 0.244 ± 0.097
0.58TrpArg: 0.58 ± 0.115
1.039TrpSer: 1.039 ± 0.382
0.855TrpThr: 0.855 ± 0.169
1.039TrpVal: 1.039 ± 0.181
0.275TrpTrp: 0.275 ± 0.111
0.642TrpTyr: 0.642 ± 0.184
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.536TyrAla: 2.536 ± 0.266
0.58TyrCys: 0.58 ± 0.137
2.536TyrAsp: 2.536 ± 0.262
2.872TyrGlu: 2.872 ± 0.296
1.528TyrPhe: 1.528 ± 0.24
2.016TyrGly: 2.016 ± 0.274
0.917TyrHis: 0.917 ± 0.179
2.719TyrIle: 2.719 ± 0.275
2.78TyrLys: 2.78 ± 0.379
2.689TyrLeu: 2.689 ± 0.365
1.253TyrMet: 1.253 ± 0.205
1.955TyrAsn: 1.955 ± 0.222
1.314TyrPro: 1.314 ± 0.231
1.283TyrGln: 1.283 ± 0.221
2.2TyrArg: 2.2 ± 0.261
2.719TyrSer: 2.719 ± 0.287
2.322TyrThr: 2.322 ± 0.298
1.986TyrVal: 1.986 ± 0.23
0.306TyrTrp: 0.306 ± 0.104
1.589TyrTyr: 1.589 ± 0.209
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 168 proteins (32733 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski