Marinoscillum sp. 108

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Cytophagia; Cytophagales; Reichenbachiellaceae; Marinoscillum; unclassified Marinoscillum

Average proteome isoelectric point is 6.05

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4475 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A654DYU8|A0A654DYU8_9BACT Uncharacterized protein OS=Marinoscillum sp. 108 OX=2653151 GN=MARINOS108_11871 PE=4 SV=1
MM1 pKa = 7.59LISSKK6 pKa = 10.14IYY8 pKa = 10.28RR9 pKa = 11.84FTFLLSLFSLLLTIACGGGDD29 pKa = 3.82GDD31 pKa = 6.11DD32 pKa = 3.98EE33 pKa = 4.79PEE35 pKa = 4.05PTAEE39 pKa = 4.8GILINEE45 pKa = 4.61IVASGDD51 pKa = 3.31DD52 pKa = 3.86WIEE55 pKa = 3.97LYY57 pKa = 10.7NALEE61 pKa = 4.12TSKK64 pKa = 10.92DD65 pKa = 3.42LSGYY69 pKa = 8.32TISDD73 pKa = 3.29SGNEE77 pKa = 4.04YY78 pKa = 10.33EE79 pKa = 5.34LPAGTTIAARR89 pKa = 11.84GFVVLLCNDD98 pKa = 4.25LGTGLNTNFKK108 pKa = 9.94LSSSGEE114 pKa = 4.28SVSLKK119 pKa = 10.15NAEE122 pKa = 4.05GTLIDD127 pKa = 3.97QVEE130 pKa = 4.67FPNLDD135 pKa = 3.23NGQSYY140 pKa = 11.4ARR142 pKa = 11.84FPDD145 pKa = 5.22GADD148 pKa = 2.68LWQITGVTTQGEE160 pKa = 5.04SNGSDD165 pKa = 3.3GAPAINSISRR175 pKa = 11.84SPLVPALNEE184 pKa = 4.2NVVVTAEE191 pKa = 4.64LISTTGVASVKK202 pKa = 10.21LFHH205 pKa = 7.05RR206 pKa = 11.84FNGSAFSEE214 pKa = 4.62VAMTLQSGTSYY225 pKa = 10.46TGTIPGMATEE235 pKa = 4.61GTVEE239 pKa = 4.29YY240 pKa = 10.4YY241 pKa = 10.57VEE243 pKa = 4.34AVGTNGSSSFKK254 pKa = 10.1PASAPDD260 pKa = 3.51NTDD263 pKa = 4.03DD264 pKa = 4.55YY265 pKa = 11.85LLNTDD270 pKa = 5.09PLPQLVINEE279 pKa = 3.99FMASNTSCCPDD290 pKa = 3.32TDD292 pKa = 3.77SGEE295 pKa = 4.83DD296 pKa = 4.07EE297 pKa = 4.21FDD299 pKa = 3.31DD300 pKa = 4.6WIEE303 pKa = 3.77IHH305 pKa = 6.47NLGATAVNLADD316 pKa = 4.67MYY318 pKa = 11.37FSDD321 pKa = 5.83DD322 pKa = 3.53KK323 pKa = 11.76DD324 pKa = 3.71NPFGDD329 pKa = 4.91KK330 pKa = 10.59ISDD333 pKa = 3.69DD334 pKa = 3.82DD335 pKa = 4.4AAATTIPAGGYY346 pKa = 9.7LVFWADD352 pKa = 3.36GSTSQGPTHH361 pKa = 6.93LNFSLSADD369 pKa = 3.79GEE371 pKa = 4.72DD372 pKa = 3.36VGLFYY377 pKa = 10.4IDD379 pKa = 3.81GRR381 pKa = 11.84TIDD384 pKa = 4.24AYY386 pKa = 9.9TFGAQSEE393 pKa = 4.45NVSWGRR399 pKa = 11.84ITDD402 pKa = 4.44GGDD405 pKa = 2.59TWGAQATPTPGQANQQ420 pKa = 3.17

Molecular weight: 44.36 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A654E699|A0A654E699_9BACT DinB family protein OS=Marinoscillum sp. 108 OX=2653151 GN=MARINOS108_90125 PE=4 SV=1
MM1 pKa = 6.86IRR3 pKa = 11.84KK4 pKa = 9.33RR5 pKa = 11.84FITVVIRR12 pKa = 11.84IYY14 pKa = 11.06LNNLHH19 pKa = 6.67LEE21 pKa = 4.29KK22 pKa = 10.69EE23 pKa = 4.42QTTLTQTANSICHH36 pKa = 6.52LADD39 pKa = 3.19NLSYY43 pKa = 10.96LRR45 pKa = 11.84RR46 pKa = 11.84QKK48 pKa = 11.01GRR50 pKa = 11.84ICIKK54 pKa = 10.0IEE56 pKa = 3.85RR57 pKa = 11.84LNTIIPRR64 pKa = 11.84GYY66 pKa = 10.74

Molecular weight: 7.86 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4475

0

4475

1608423

24

6157

359.4

40.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.94 ± 0.032

0.692 ± 0.012

5.828 ± 0.035

6.62 ± 0.035

4.923 ± 0.027

7.247 ± 0.044

1.928 ± 0.019

6.915 ± 0.035

5.837 ± 0.052

9.558 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.379 ± 0.018

5.004 ± 0.034

3.756 ± 0.022

3.648 ± 0.021

4.053 ± 0.027

7.008 ± 0.045

5.788 ± 0.045

6.572 ± 0.026

1.25 ± 0.013

4.054 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski