Streptococcus infantis SK970
Average proteome isoelectric point is 6.19
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2144 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|F9PUJ1|F9PUJ1_9STRE ABC transporter permease protein OS=Streptococcus infantis SK970 OX=1035189 GN=HMPREF9954_0895 PE=3 SV=1
MM1 pKa = 7.28 KK2 pKa = 9.87 RR3 pKa = 11.84 DD4 pKa = 3.37 EE5 pKa = 5.08 FGFILPNLYY14 pKa = 10.4 YY15 pKa = 10.62 QFLTEE20 pKa = 4.68 LKK22 pKa = 10.21 EE23 pKa = 3.69 IDD25 pKa = 3.98 PYY27 pKa = 11.28 EE28 pKa = 5.54 IGDD31 pKa = 4.33 SGICLYY37 pKa = 10.78 AQEE40 pKa = 5.16 DD41 pKa = 3.74 LQEE44 pKa = 4.4 RR45 pKa = 11.84 NEE47 pKa = 4.19 TYY49 pKa = 10.3 QIEE52 pKa = 4.57 EE53 pKa = 4.14 VEE55 pKa = 4.17 PDD57 pKa = 3.28 YY58 pKa = 11.59 FMIGQEE64 pKa = 3.56 GDD66 pKa = 2.95 LAYY69 pKa = 10.23 FIKK72 pKa = 10.68 KK73 pKa = 10.41 NSDD76 pKa = 3.05 DD77 pKa = 4.51 CIYY80 pKa = 10.94 EE81 pKa = 4.03 NDD83 pKa = 3.68 LGALGSLEE91 pKa = 4.12 MQKK94 pKa = 10.32 VAANVYY100 pKa = 10.61 DD101 pKa = 4.89 FIEE104 pKa = 4.4 KK105 pKa = 9.97 VLKK108 pKa = 10.81 EE109 pKa = 4.04 EE110 pKa = 4.26 LL111 pKa = 3.56
Molecular weight: 13.02 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.778
IPC2_protein 3.986
IPC_protein 3.91
Toseland 3.732
ProMoST 4.037
Dawson 3.872
Bjellqvist 4.024
Wikipedia 3.757
Rodwell 3.745
Grimsley 3.643
Solomon 3.859
Lehninger 3.821
Nozaki 3.986
DTASelect 4.126
Thurlkill 3.757
EMBOSS 3.77
Sillero 4.024
Patrickios 0.998
IPC_peptide 3.859
IPC2_peptide 4.012
IPC2.peptide.svr19 3.922
Protein with the highest isoelectric point:
>tr|F9PZ73|F9PZ73_9STRE SseB domain-containing protein OS=Streptococcus infantis SK970 OX=1035189 GN=HMPREF9954_0345 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 10.24 QPSKK9 pKa = 9.82 LRR11 pKa = 11.84 RR12 pKa = 11.84 ARR14 pKa = 11.84 KK15 pKa = 8.57 HH16 pKa = 4.75 GFRR19 pKa = 11.84 NRR21 pKa = 11.84 MSTKK25 pKa = 9.22 NGRR28 pKa = 11.84 RR29 pKa = 11.84 VLAARR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 KK37 pKa = 8.87 GRR39 pKa = 11.84 KK40 pKa = 8.75 VLAAA44 pKa = 4.31
Molecular weight: 5.27 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.449
IPC2_protein 11.155
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.398
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.135
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.082
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2144
0
2144
564361
37
2882
263.2
29.55
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.409 ± 0.061
0.543 ± 0.017
5.738 ± 0.043
7.486 ± 0.063
4.486 ± 0.052
6.573 ± 0.055
1.831 ± 0.024
7.06 ± 0.062
6.949 ± 0.059
9.815 ± 0.09
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.461 ± 0.029
4.499 ± 0.038
3.445 ± 0.049
4.067 ± 0.043
4.035 ± 0.036
6.08 ± 0.05
5.844 ± 0.081
6.987 ± 0.047
0.922 ± 0.017
3.767 ± 0.042
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here