Puniceispirillum marinum (strain IMCC1322)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Alphaproteobacteria incertae sedis; SAR116 cluster; Candidatus Puniceispirillum; Candidatus Puniceispirillum marinum

Average proteome isoelectric point is 6.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2545 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D5BQZ7|D5BQZ7_PUNMI Cation antiporter OS=Puniceispirillum marinum (strain IMCC1322) OX=488538 GN=SAR116_0467 PE=3 SV=1
MM1 pKa = 7.28VLNKK5 pKa = 9.53MSEE8 pKa = 4.44IISKK12 pKa = 7.94TANSEE17 pKa = 4.28TVTPSSMAGIFSLTEE32 pKa = 3.5AAADD36 pKa = 4.87RR37 pKa = 11.84ITSMLAAEE45 pKa = 4.55PDD47 pKa = 3.42GSFFRR52 pKa = 11.84VAVLGGGCSGFQYY65 pKa = 10.52QFSIDD70 pKa = 3.69TNRR73 pKa = 11.84DD74 pKa = 3.08DD75 pKa = 3.96EE76 pKa = 4.73DD77 pKa = 3.96QVFTSHH83 pKa = 6.23NVDD86 pKa = 3.72VIIDD90 pKa = 3.81EE91 pKa = 4.38MSLEE95 pKa = 4.25LVDD98 pKa = 4.98HH99 pKa = 7.19AEE101 pKa = 3.85LDD103 pKa = 4.08YY104 pKa = 11.61VQDD107 pKa = 3.64LMGSYY112 pKa = 10.21FAVNNPNATASCGCGTSFSVV132 pKa = 3.97

Molecular weight: 14.14 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D5BT02|D5BT02_PUNMI CDP-diacylglycerol--serine O-phosphatidyltransferase OS=Puniceispirillum marinum (strain IMCC1322) OX=488538 GN=SAR116_1156 PE=3 SV=1
MM1 pKa = 7.61FKK3 pKa = 10.68RR4 pKa = 11.84FIRR7 pKa = 11.84NKK9 pKa = 9.6FVIWCLSWVVAGIIASIMITTRR31 pKa = 11.84WKK33 pKa = 8.83TVNRR37 pKa = 11.84QLADD41 pKa = 3.48QILNNHH47 pKa = 6.18NGFVLVLWHH56 pKa = 6.07EE57 pKa = 4.81RR58 pKa = 11.84IFAVPWLWPRR68 pKa = 11.84RR69 pKa = 11.84HH70 pKa = 6.06PMYY73 pKa = 10.71VLQSPHH79 pKa = 7.08ADD81 pKa = 3.54GQLMSYY87 pKa = 8.11TVNRR91 pKa = 11.84MGIRR95 pKa = 11.84TVWGSSNRR103 pKa = 11.84NAISGLRR110 pKa = 11.84GLKK113 pKa = 9.83RR114 pKa = 11.84VLDD117 pKa = 4.19RR118 pKa = 11.84GQIATITPDD127 pKa = 3.45GPRR130 pKa = 11.84GPARR134 pKa = 11.84KK135 pKa = 8.74LAIGPVALAAMAGKK149 pKa = 9.15PVVPICWAVDD159 pKa = 3.98RR160 pKa = 11.84YY161 pKa = 8.84WRR163 pKa = 11.84APGWDD168 pKa = 3.32GTIIPKK174 pKa = 9.91PFARR178 pKa = 11.84GRR180 pKa = 11.84FIWGDD185 pKa = 3.69PIDD188 pKa = 4.11VPKK191 pKa = 10.82GDD193 pKa = 4.32RR194 pKa = 11.84DD195 pKa = 3.63TMEE198 pKa = 3.91TSRR201 pKa = 11.84VEE203 pKa = 3.99IEE205 pKa = 3.86TAMNDD210 pKa = 3.35LADD213 pKa = 4.08RR214 pKa = 11.84ADD216 pKa = 3.76RR217 pKa = 11.84LFAEE221 pKa = 4.62EE222 pKa = 4.77DD223 pKa = 3.61GLRR226 pKa = 3.71

Molecular weight: 25.72 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2545

0

2545

832913

33

8246

327.3

35.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.876 ± 0.063

0.993 ± 0.02

6.609 ± 0.057

5.037 ± 0.042

3.993 ± 0.038

7.84 ± 0.046

2.329 ± 0.027

6.598 ± 0.044

4.153 ± 0.038

9.445 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.09 ± 0.035

3.59 ± 0.046

4.402 ± 0.031

3.363 ± 0.027

5.413 ± 0.041

6.167 ± 0.037

5.616 ± 0.071

6.82 ± 0.038

1.217 ± 0.02

2.451 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski