Prevotella sp. oral taxon 473 str. F0040 
Average proteome isoelectric point is 6.83 
Get precalculated fractions of proteins 
 
  
    Acidic  
     
   
 
  
    pI < 6.8  
     
   
 
  
    6.8-7.4  
     
   
 
  
    pI > 7.4  
     
   
 
  
    Basic  
     
   
    
 
  
    All  
     
   
 
 
Note: above files contain also dissociation constants (pKa) 
 
Virtual 2D-PAGE plot for 2316 proteins (isoelectric point calculated using IPC2_protein) 
 
Get csv file with sequences according to given criteria: 
* You can choose from 21 different methods for calculating isoelectric point 
 
 Summary statistics related to proteome-wise predictions 
 
Protein with the lowest isoelectric point: 
>tr|L1MHT0|L1MHT0_9BACT Lipocalin-like domain-containing protein OS=Prevotella sp. oral taxon 473 str. F0040 OX=1035197 GN=HMPREF9999_01301 PE=4 SV=1 
MM1 pKa = 7.6  LLASCAVRR9 pKa = 11.84  GKK11 pKa = 10.72  YY12 pKa = 10.1  SEE14 pKa = 5.5  DD15 pKa = 3.44  DD16 pKa = 3.78  AEE18 pKa = 4.66  TADD21 pKa = 3.9  SAYY24 pKa = 9.2  DD25 pKa = 3.61  TSDD28 pKa = 3.34  DD29 pKa = 3.98  SQGNNHH35 pKa = 6.68  DD36 pKa = 4.36  AEE38 pKa = 5.22  DD39 pKa = 4.02  NFSGASSRR47 pKa = 11.84  HH48 pKa = 5.29  MDD50 pKa = 3.36  SEE52 pKa = 4.59  DD53 pKa = 3.68  NEE55 pKa = 4.73  GSSTMMSDD63 pKa = 3.0  RR64 pKa = 11.84  LSPEE68 pKa = 3.83  DD69 pKa = 3.34  EE70 pKa = 4.57  AYY72 pKa = 9.85  EE73 pKa = 4.38  AGHH76 pKa = 5.58  SQGYY80 pKa = 10.5  DD81 pKa = 3.31  DD82 pKa = 5.34  GSADD86 pKa = 3.66  AGTGSDD92 pKa = 3.62  HH93 pKa = 5.89  GTSFDD98 pKa = 4.56  EE99 pKa = 4.56  DD100 pKa = 2.91  NKK102 pKa = 9.99  YY103 pKa = 10.0  QGKK106 pKa = 9.24  AAEE109 pKa = 4.27  QYY111 pKa = 10.69  EE112 pKa = 4.27  EE113 pKa = 5.32  GYY115 pKa = 9.86  NAGYY119 pKa = 10.74  NEE121 pKa = 4.38  GFNEE125 pKa = 4.14  LTRR128 pKa = 4.63   
 Molecular weight: 13.78 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  3.705 
IPC2_protein 3.808 
IPC_protein 3.808 
Toseland    3.592 
ProMoST     3.961 
Dawson      3.795 
Bjellqvist  3.948 
Wikipedia   3.719 
Rodwell     3.63 
Grimsley    3.503 
Solomon     3.783 
Lehninger   3.732 
Nozaki      3.91 
DTASelect   4.126 
Thurlkill   3.643 
EMBOSS      3.732 
Sillero     3.923 
Patrickios  1.062 
IPC_peptide 3.783 
IPC2_peptide  3.897 
IPC2.peptide.svr19  3.82 
 Protein with the highest isoelectric point: 
>tr|L1MAM7|L1MAM7_9BACT N-acetylglucosamine-6-phosphate deacetylase OS=Prevotella sp. oral taxon 473 str. F0040 OX=1035197 GN=HMPREF9999_02052 PE=3 SV=1 
MM1 pKa = 7.34  SVLRR5 pKa = 11.84  FTARR9 pKa = 11.84  LCLQRR14 pKa = 11.84  YY15 pKa = 9.74  DD16 pKa = 4.0  EE17 pKa = 5.1  FVTLQSSRR25 pKa = 11.84  EE26 pKa = 3.86  QQGNIGNFKK35 pKa = 10.62  EE36 pKa = 4.32  KK37 pKa = 9.15  LTHH40 pKa = 7.16  FIGSRR45 pKa = 11.84  LRR47 pKa = 11.84  ILSLFSSFAVCLAQRR62 pKa = 3.89   
 Molecular weight: 7.21 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  9.34 
IPC2_protein 9.385 
IPC_protein 10.087 
Toseland    10.423 
ProMoST     10.789 
Dawson      10.54 
Bjellqvist  10.277 
Wikipedia   10.745 
Rodwell     10.687 
Grimsley    10.584 
Solomon     10.672 
Lehninger   10.643 
Nozaki      10.482 
DTASelect   10.248 
Thurlkill   10.438 
EMBOSS      10.818 
Sillero     10.496 
Patrickios  10.599 
IPC_peptide 10.672 
IPC2_peptide  9.648 
IPC2.peptide.svr19  8.695 
  
 
Peptides (in silico  digests for buttom-up proteomics) 
Below you can find 
in silico  digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
 
  
    Try ESI  
     
   
 
  
    ChTry ESI  
     
   
 
  
    ArgC ESI  
     
   
 
  
    LysN ESI  
     
   
 
  
    TryLysC ESI  
     
   
 
  
    Try MALDI  
     
   
 
  
    ChTry MALDI  
     
   
 
  
    ArgC MALDI  
     
   
 
  
    LysN MALDI  
     
   
 
  
    TryLysC MALDI  
     
   
 
  
    Try LTQ  
     
   
 
  
    ChTry LTQ  
     
   
 
  
    ArgC LTQ  
     
   
 
  
    LysN LTQ  
     
   
 
  
    TryLysC LTQ  
     
   
 
  
    Try MSlow  
     
   
 
  
    ChTry MSlow  
     
   
 
  
    ArgC MSlow  
     
   
 
  
    LysN MSlow  
     
   
 
  
    TryLysC MSlow  
     
   
 
  
    Try MShigh  
     
   
 
  
    ChTry MShigh  
     
   
 
  
    ArgC MShigh  
     
   
 
  
    LysN MShigh  
     
   
 
  
    TryLysC MShigh  
     
   
   
  
                       
General Statistics 
    
      
        Number of major isoforms
 
        Number of additional isoforms
 
        Number of all proteins
         
        Number of amino acids
 
        Min. Seq. Length
 
        Max. Seq. Length
 
        Avg. Seq. Length
 
        Avg. Mol. Weight
 
       
     
    
      
        2316 
 
        
        0
 
        
        2316 
         
        734294
 
        38
 
        2495
 
        317.1
 
        35.61
 
                
     
  
 
    Amino acid frequency 
  
  
    
      
        Ala
 
        Cys
 
        Asp
 
        Glu
 
        Phe
 
        Gly
 
        His
 
        Ile
 
        Lys
 
        Leu
 
       
     
    
                 
        8.248 ± 0.052
1.119 ± 0.021
 
        5.242 ± 0.036
6.192 ± 0.054
 
        4.576 ± 0.036
6.629 ± 0.049
 
        2.374 ± 0.03
5.795 ± 0.049
       
        5.58 ± 0.047
9.759 ± 0.061
         
                
     
  
  
  
      
          
        Met
         
        Asn
 
        Gln
 
        Pro
         
        Arg
 
        Ser
 
        Thr
 
        Val
 
        Trp
 
        Tyr
  
       
     
    
                 
        2.487 ± 0.025
4.562 ± 0.048
 
        3.98 ± 0.032
4.222 ± 0.033
 
        5.358 ± 0.04
6.307 ± 0.044
 
        5.792 ± 0.04
6.682 ± 0.044
       
        1.244 ± 0.019
3.852 ± 0.036
         
                
     
  
  
  
Note:  For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level 
Most of the basic statistics you can see at this page can be downloaded from this CSV file  
For dipeptide frequency statistics click here