Prevotella sp. oral taxon 473 str. F0040
Average proteome isoelectric point is 6.83
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2316 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|L1MHT0|L1MHT0_9BACT Lipocalin-like domain-containing protein OS=Prevotella sp. oral taxon 473 str. F0040 OX=1035197 GN=HMPREF9999_01301 PE=4 SV=1
MM1 pKa = 7.6 LLASCAVRR9 pKa = 11.84 GKK11 pKa = 10.72 YY12 pKa = 10.1 SEE14 pKa = 5.5 DD15 pKa = 3.44 DD16 pKa = 3.78 AEE18 pKa = 4.66 TADD21 pKa = 3.9 SAYY24 pKa = 9.2 DD25 pKa = 3.61 TSDD28 pKa = 3.34 DD29 pKa = 3.98 SQGNNHH35 pKa = 6.68 DD36 pKa = 4.36 AEE38 pKa = 5.22 DD39 pKa = 4.02 NFSGASSRR47 pKa = 11.84 HH48 pKa = 5.29 MDD50 pKa = 3.36 SEE52 pKa = 4.59 DD53 pKa = 3.68 NEE55 pKa = 4.73 GSSTMMSDD63 pKa = 3.0 RR64 pKa = 11.84 LSPEE68 pKa = 3.83 DD69 pKa = 3.34 EE70 pKa = 4.57 AYY72 pKa = 9.85 EE73 pKa = 4.38 AGHH76 pKa = 5.58 SQGYY80 pKa = 10.5 DD81 pKa = 3.31 DD82 pKa = 5.34 GSADD86 pKa = 3.66 AGTGSDD92 pKa = 3.62 HH93 pKa = 5.89 GTSFDD98 pKa = 4.56 EE99 pKa = 4.56 DD100 pKa = 2.91 NKK102 pKa = 9.99 YY103 pKa = 10.0 QGKK106 pKa = 9.24 AAEE109 pKa = 4.27 QYY111 pKa = 10.69 EE112 pKa = 4.27 EE113 pKa = 5.32 GYY115 pKa = 9.86 NAGYY119 pKa = 10.74 NEE121 pKa = 4.38 GFNEE125 pKa = 4.14 LTRR128 pKa = 4.63
Molecular weight: 13.78 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.705
IPC2_protein 3.808
IPC_protein 3.808
Toseland 3.592
ProMoST 3.961
Dawson 3.795
Bjellqvist 3.948
Wikipedia 3.719
Rodwell 3.63
Grimsley 3.503
Solomon 3.783
Lehninger 3.732
Nozaki 3.91
DTASelect 4.126
Thurlkill 3.643
EMBOSS 3.732
Sillero 3.923
Patrickios 1.062
IPC_peptide 3.783
IPC2_peptide 3.897
IPC2.peptide.svr19 3.82
Protein with the highest isoelectric point:
>tr|L1MAM7|L1MAM7_9BACT N-acetylglucosamine-6-phosphate deacetylase OS=Prevotella sp. oral taxon 473 str. F0040 OX=1035197 GN=HMPREF9999_02052 PE=3 SV=1
MM1 pKa = 7.34 SVLRR5 pKa = 11.84 FTARR9 pKa = 11.84 LCLQRR14 pKa = 11.84 YY15 pKa = 9.74 DD16 pKa = 4.0 EE17 pKa = 5.1 FVTLQSSRR25 pKa = 11.84 EE26 pKa = 3.86 QQGNIGNFKK35 pKa = 10.62 EE36 pKa = 4.32 KK37 pKa = 9.15 LTHH40 pKa = 7.16 FIGSRR45 pKa = 11.84 LRR47 pKa = 11.84 ILSLFSSFAVCLAQRR62 pKa = 3.89
Molecular weight: 7.21 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.34
IPC2_protein 9.385
IPC_protein 10.087
Toseland 10.423
ProMoST 10.789
Dawson 10.54
Bjellqvist 10.277
Wikipedia 10.745
Rodwell 10.687
Grimsley 10.584
Solomon 10.672
Lehninger 10.643
Nozaki 10.482
DTASelect 10.248
Thurlkill 10.438
EMBOSS 10.818
Sillero 10.496
Patrickios 10.599
IPC_peptide 10.672
IPC2_peptide 9.648
IPC2.peptide.svr19 8.695
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2316
0
2316
734294
38
2495
317.1
35.61
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.248 ± 0.052
1.119 ± 0.021
5.242 ± 0.036
6.192 ± 0.054
4.576 ± 0.036
6.629 ± 0.049
2.374 ± 0.03
5.795 ± 0.049
5.58 ± 0.047
9.759 ± 0.061
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.487 ± 0.025
4.562 ± 0.048
3.98 ± 0.032
4.222 ± 0.033
5.358 ± 0.04
6.307 ± 0.044
5.792 ± 0.04
6.682 ± 0.044
1.244 ± 0.019
3.852 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here