Paraburkholderia sacchari

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Burkholderiaceae; Paraburkholderia

Average proteome isoelectric point is 6.67

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5729 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0A6QBR2|A0A0A6QBR2_9BURK 3-dmu-9_3-mt domain-containing protein OS=Paraburkholderia sacchari OX=159450 GN=NH14_12980 PE=4 SV=1
MM1 pKa = 7.08ATAAHH6 pKa = 6.62ATQSDD11 pKa = 3.79QAFKK15 pKa = 10.8QWVCVICGWVYY26 pKa = 11.39DD27 pKa = 4.52EE28 pKa = 5.71AAGAPDD34 pKa = 5.29DD35 pKa = 4.4GLAPGTRR42 pKa = 11.84WADD45 pKa = 3.28VPNDD49 pKa = 3.68WRR51 pKa = 11.84CPLCDD56 pKa = 3.32VGKK59 pKa = 10.7EE60 pKa = 3.91DD61 pKa = 3.78FALVEE66 pKa = 4.41FF67 pKa = 4.99

Molecular weight: 7.32 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0A6Q7X3|A0A0A6Q7X3_9BURK Flagellar motor switch protein FliG OS=Paraburkholderia sacchari OX=159450 GN=fliG PE=3 SV=1
MM1 pKa = 7.46HH2 pKa = 7.51SLRR5 pKa = 11.84ATRR8 pKa = 11.84AANKK12 pKa = 9.62LLHH15 pKa = 6.75RR16 pKa = 11.84LATLLLLRR24 pKa = 11.84HH25 pKa = 6.29RR26 pKa = 11.84LLLLRR31 pKa = 11.84TQVLLLRR38 pKa = 11.84APLLLRR44 pKa = 11.84AKK46 pKa = 10.35LRR48 pKa = 11.84LLRR51 pKa = 11.84ATLPLRR57 pKa = 11.84LPLLLRR63 pKa = 11.84TLALLLRR70 pKa = 11.84LRR72 pKa = 11.84AQASKK77 pKa = 10.55LYY79 pKa = 10.82SRR81 pKa = 11.84GARR84 pKa = 11.84PKK86 pKa = 10.67LRR88 pKa = 11.84LQRR91 pKa = 11.84ASKK94 pKa = 9.03RR95 pKa = 11.84TAGRR99 pKa = 11.84SDD101 pKa = 3.23GLVRR105 pKa = 11.84RR106 pKa = 11.84AAGKK110 pKa = 9.87QKK112 pKa = 9.57SHH114 pKa = 6.25EE115 pKa = 4.02PRR117 pKa = 11.84FVAFLLHH124 pKa = 6.15PLVRR128 pKa = 5.22

Molecular weight: 14.7 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5729

0

5729

1829930

38

3400

319.4

34.65

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.023 ± 0.055

0.968 ± 0.01

5.278 ± 0.026

5.282 ± 0.032

3.714 ± 0.02

8.31 ± 0.034

2.341 ± 0.017

4.681 ± 0.025

3.114 ± 0.027

10.098 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.433 ± 0.014

2.857 ± 0.024

5.044 ± 0.023

3.617 ± 0.023

6.888 ± 0.037

5.459 ± 0.027

5.289 ± 0.03

7.756 ± 0.023

1.384 ± 0.013

2.463 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski