Pseudoflavonifractor sp. BIOML-A4

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Oscillospiraceae; Pseudoflavonifractor; unclassified Pseudoflavonifractor

Average proteome isoelectric point is 6.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4105 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6I3RAU3|A0A6I3RAU3_9FIRM Uncharacterized protein OS=Pseudoflavonifractor sp. BIOML-A4 OX=2584666 GN=GMD93_13595 PE=4 SV=1
MM1 pKa = 7.86LKK3 pKa = 10.35RR4 pKa = 11.84KK5 pKa = 9.56LIPLALTLGLLLSCAACGEE24 pKa = 4.61TAGGTASPSSTPSASPSSAPAAGEE48 pKa = 3.95TSIVLSDD55 pKa = 3.71GGVTVDD61 pKa = 4.26GAAASTDD68 pKa = 3.31AGSAVYY74 pKa = 10.16VGAEE78 pKa = 3.91IIYY81 pKa = 9.82YY82 pKa = 9.61EE83 pKa = 4.19AGRR86 pKa = 11.84DD87 pKa = 3.45EE88 pKa = 5.11SYY90 pKa = 11.41GAGAEE95 pKa = 4.28ADD97 pKa = 3.28GHH99 pKa = 5.18TAGEE103 pKa = 4.15AAEE106 pKa = 4.09QTVVTITQPGTYY118 pKa = 8.61RR119 pKa = 11.84VSGTLSAGQLAVDD132 pKa = 5.01LGEE135 pKa = 5.14DD136 pKa = 3.58AQSDD140 pKa = 3.69PDD142 pKa = 3.86AVVTLILDD150 pKa = 3.83NMDD153 pKa = 3.09ITCAVAPAVIFYY165 pKa = 10.66SVYY168 pKa = 9.85EE169 pKa = 4.43CGSADD174 pKa = 3.86TEE176 pKa = 4.45TTAATVDD183 pKa = 3.64TSAAGANVIIADD195 pKa = 3.76GSVNHH200 pKa = 6.24VDD202 pKa = 3.14GSYY205 pKa = 10.08VAKK208 pKa = 10.18IYY210 pKa = 10.67QEE212 pKa = 4.03GTEE215 pKa = 4.39KK216 pKa = 10.81KK217 pKa = 9.04LHH219 pKa = 6.42KK220 pKa = 10.22YY221 pKa = 10.51DD222 pKa = 3.19GAFYY226 pKa = 11.07SKK228 pKa = 10.46MSMNVQGEE236 pKa = 4.09AAGDD240 pKa = 3.8GEE242 pKa = 5.35LYY244 pKa = 8.86ITAANEE250 pKa = 4.05GLDD253 pKa = 3.95SEE255 pKa = 4.77LHH257 pKa = 5.07LTINGGNISITAQNDD272 pKa = 4.74GINTNEE278 pKa = 4.79DD279 pKa = 3.54GVSVTTVNGGRR290 pKa = 11.84LYY292 pKa = 10.65INAGLGDD299 pKa = 4.28EE300 pKa = 4.9GDD302 pKa = 4.78GIDD305 pKa = 3.54SNGYY309 pKa = 9.23LVINGGEE316 pKa = 4.03IVTLANGRR324 pKa = 11.84SGDD327 pKa = 3.61GGLDD331 pKa = 3.42ADD333 pKa = 4.47QGIYY337 pKa = 10.8LNGGSVLALGSRR349 pKa = 11.84NDD351 pKa = 3.6AASTDD356 pKa = 3.52SAQAYY361 pKa = 8.41MEE363 pKa = 4.54LAFSSTLPAGSVVSITDD380 pKa = 3.25AEE382 pKa = 4.47GNEE385 pKa = 4.15LVSYY389 pKa = 8.78TAEE392 pKa = 4.22KK393 pKa = 10.7EE394 pKa = 4.26FATLTYY400 pKa = 8.03TAPALRR406 pKa = 11.84DD407 pKa = 3.33DD408 pKa = 4.4AVYY411 pKa = 10.08HH412 pKa = 5.78VYY414 pKa = 10.77VDD416 pKa = 4.5GVQQQFTGHH425 pKa = 5.77SFGMGGGMMGGQEE438 pKa = 3.88PPEE441 pKa = 4.36GFEE444 pKa = 4.24HH445 pKa = 6.02GQEE448 pKa = 4.3GEE450 pKa = 4.2RR451 pKa = 11.84PDD453 pKa = 4.62LPEE456 pKa = 4.73GMEE459 pKa = 4.12PPEE462 pKa = 4.57DD463 pKa = 4.15FEE465 pKa = 6.28PGQQPDD471 pKa = 3.81GTGMPRR477 pKa = 11.84GTAPADD483 pKa = 4.11DD484 pKa = 4.86GDD486 pKa = 3.86RR487 pKa = 11.84TPPGGAPDD495 pKa = 3.76GSGRR499 pKa = 11.84GGGRR503 pKa = 11.84GDD505 pKa = 3.67RR506 pKa = 11.84GEE508 pKa = 4.24TLDD511 pKa = 3.99PSAEE515 pKa = 4.03FSLSAGSHH523 pKa = 5.4SFSGVGDD530 pKa = 3.78STLGG534 pKa = 3.34

Molecular weight: 53.86 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6I3RCE7|A0A6I3RCE7_9FIRM DUF4314 domain-containing protein OS=Pseudoflavonifractor sp. BIOML-A4 OX=2584666 GN=GMD93_06560 PE=4 SV=1
SS1 pKa = 7.18RR2 pKa = 11.84GGGSFGGGGSRR13 pKa = 11.84GGGFGGGSRR22 pKa = 11.84GGGFGGRR29 pKa = 11.84RR30 pKa = 3.33

Molecular weight: 2.62 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4105

0

4105

1302255

18

4012

317.2

34.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.197 ± 0.054

1.586 ± 0.02

5.7 ± 0.034

6.576 ± 0.039

3.822 ± 0.027

8.579 ± 0.047

1.687 ± 0.017

5.324 ± 0.037

4.411 ± 0.034

9.775 ± 0.06

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.661 ± 0.02

3.335 ± 0.035

4.219 ± 0.03

3.001 ± 0.02

5.808 ± 0.055

5.605 ± 0.031

5.679 ± 0.05

7.322 ± 0.034

1.127 ± 0.017

3.587 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski