Amino acid dipepetide frequency for Pseudoflavonifractor sp. BIOML-A4

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	13.965AlaAla: 13.965 ± 0.184	1.563AlaCys: 1.563 ± 0.034	6.012AlaAsp: 6.012 ± 0.093	6.895AlaGlu: 6.895 ± 0.074	3.612AlaPhe: 3.612 ± 0.058	9.276AlaGly: 9.276 ± 0.112	1.488AlaHis: 1.488 ± 0.038	4.586AlaIle: 4.586 ± 0.072	3.793AlaLys: 3.793 ± 0.059	11.071AlaLeu: 11.071 ± 0.124	2.752AlaMet: 2.752 ± 0.049	2.536AlaAsn: 2.536 ± 0.054	4.016AlaPro: 4.016 ± 0.065	3.181AlaGln: 3.181 ± 0.057	5.549AlaArg: 5.549 ± 0.087	4.59AlaSer: 4.59 ± 0.064	3.886AlaThr: 3.886 ± 0.069	9.173AlaVal: 9.173 ± 0.101	1.096AlaTrp: 1.096 ± 0.03	2.927AlaTyr: 2.927 ± 0.052	0.0AlaXaa: 0.0 ± 0.0
Cys	1.682CysAla: 1.682 ± 0.036	0.323CysCys: 0.323 ± 0.015	0.84CysAsp: 0.84 ± 0.027	0.832CysGlu: 0.832 ± 0.029	0.63CysPhe: 0.63 ± 0.023	1.839CysGly: 1.839 ± 0.045	0.326CysHis: 0.326 ± 0.016	0.835CysIle: 0.835 ± 0.027	0.518CysLys: 0.518 ± 0.024	1.342CysLeu: 1.342 ± 0.036	0.379CysMet: 0.379 ± 0.015	0.431CysAsn: 0.431 ± 0.02	0.835CysPro: 0.835 ± 0.03	0.374CysGln: 0.374 ± 0.019	1.08CysArg: 1.08 ± 0.033	0.812CysSer: 0.812 ± 0.028	0.928CysThr: 0.928 ± 0.027	1.126CysVal: 1.126 ± 0.03	0.177CysTrp: 0.177 ± 0.011	0.548CysTyr: 0.548 ± 0.025	0.0CysXaa: 0.0 ± 0.0
Asp	5.823AspAla: 5.823 ± 0.083	0.949AspCys: 0.949 ± 0.026	2.739AspAsp: 2.739 ± 0.05	3.747AspGlu: 3.747 ± 0.061	2.489AspPhe: 2.489 ± 0.042	6.043AspGly: 6.043 ± 0.104	0.873AspHis: 0.873 ± 0.025	3.398AspIle: 3.398 ± 0.052	2.569AspLys: 2.569 ± 0.048	5.114AspLeu: 5.114 ± 0.061	1.651AspMet: 1.651 ± 0.042	1.719AspAsn: 1.719 ± 0.044	2.553AspPro: 2.553 ± 0.046	1.398AspGln: 1.398 ± 0.037	3.166AspArg: 3.166 ± 0.064	2.684AspSer: 2.684 ± 0.052	3.074AspThr: 3.074 ± 0.057	3.879AspVal: 3.879 ± 0.056	0.763AspTrp: 0.763 ± 0.029	2.364AspTyr: 2.364 ± 0.044	0.0AspXaa: 0.0 ± 0.0
Glu	6.544GluAla: 6.544 ± 0.077	0.736GluCys: 0.736 ± 0.023	3.655GluAsp: 3.655 ± 0.06	5.017GluGlu: 5.017 ± 0.081	1.946GluPhe: 1.946 ± 0.042	5.06GluGly: 5.06 ± 0.067	1.287GluHis: 1.287 ± 0.034	3.617GluIle: 3.617 ± 0.059	3.89GluLys: 3.89 ± 0.06	6.708GluLeu: 6.708 ± 0.091	1.801GluMet: 1.801 ± 0.039	2.745GluAsn: 2.745 ± 0.046	2.113GluPro: 2.113 ± 0.048	2.486GluGln: 2.486 ± 0.051	4.482GluArg: 4.482 ± 0.071	2.999GluSer: 2.999 ± 0.051	3.645GluThr: 3.645 ± 0.058	4.025GluVal: 4.025 ± 0.055	0.633GluTrp: 0.633 ± 0.02	2.368GluTyr: 2.368 ± 0.039	0.0GluXaa: 0.0 ± 0.0
Phe	3.471PheAla: 3.471 ± 0.056	0.733PheCys: 0.733 ± 0.028	2.357PheAsp: 2.357 ± 0.044	2.103PheGlu: 2.103 ± 0.04	1.713PhePhe: 1.713 ± 0.043	3.098PheGly: 3.098 ± 0.05	0.754PheHis: 0.754 ± 0.025	2.058PheIle: 2.058 ± 0.04	1.352PheLys: 1.352 ± 0.031	3.737PheLeu: 3.737 ± 0.074	0.849PheMet: 0.849 ± 0.027	1.362PheAsn: 1.362 ± 0.034	1.583PhePro: 1.583 ± 0.034	1.27PheGln: 1.27 ± 0.028	2.094PheArg: 2.094 ± 0.046	2.76PheSer: 2.76 ± 0.042	2.553PheThr: 2.553 ± 0.052	2.503PheVal: 2.503 ± 0.054	0.428PheTrp: 0.428 ± 0.019	1.44PheTyr: 1.44 ± 0.04	0.0PheXaa: 0.0 ± 0.0
Gly	8.256GlyAla: 8.256 ± 0.105	1.515GlyCys: 1.515 ± 0.039	4.69GlyAsp: 4.69 ± 0.072	5.877GlyGlu: 5.877 ± 0.079	3.334GlyPhe: 3.334 ± 0.05	8.167GlyGly: 8.167 ± 0.136	1.384GlyHis: 1.384 ± 0.035	4.582GlyIle: 4.582 ± 0.062	4.256GlyLys: 4.256 ± 0.063	7.675GlyLeu: 7.675 ± 0.09	2.584GlyMet: 2.584 ± 0.043	2.695GlyAsn: 2.695 ± 0.062	2.261GlyPro: 2.261 ± 0.064	2.405GlyGln: 2.405 ± 0.046	5.06GlyArg: 5.06 ± 0.077	4.769GlySer: 4.769 ± 0.077	4.971GlyThr: 4.971 ± 0.094	6.858GlyVal: 6.858 ± 0.073	1.11GlyTrp: 1.11 ± 0.034	3.339GlyTyr: 3.339 ± 0.053	0.0GlyXaa: 0.0 ± 0.0
His	1.361HisAla: 1.361 ± 0.034	0.315HisCys: 0.315 ± 0.015	0.865HisAsp: 0.865 ± 0.026	0.931HisGlu: 0.931 ± 0.028	0.81HisPhe: 0.81 ± 0.024	1.427HisGly: 1.427 ± 0.038	0.339HisHis: 0.339 ± 0.017	1.216HisIle: 1.216 ± 0.029	0.646HisLys: 0.646 ± 0.029	1.586HisLeu: 1.586 ± 0.04	0.483HisMet: 0.483 ± 0.018	0.62HisAsn: 0.62 ± 0.023	0.976HisPro: 0.976 ± 0.029	0.481HisGln: 0.481 ± 0.018	0.968HisArg: 0.968 ± 0.028	0.903HisSer: 0.903 ± 0.026	1.035HisThr: 1.035 ± 0.031	1.007HisVal: 1.007 ± 0.027	0.234HisTrp: 0.234 ± 0.015	0.664HisTyr: 0.664 ± 0.024	0.0HisXaa: 0.0 ± 0.0
Ile	5.027IleAla: 5.027 ± 0.073	0.918IleCys: 0.918 ± 0.029	3.075IleAsp: 3.075 ± 0.056	3.073IleGlu: 3.073 ± 0.046	1.993IlePhe: 1.993 ± 0.045	3.985IleGly: 3.985 ± 0.068	0.977IleHis: 0.977 ± 0.03	3.054IleIle: 3.054 ± 0.064	2.146IleLys: 2.146 ± 0.043	5.385IleLeu: 5.385 ± 0.082	1.246IleMet: 1.246 ± 0.033	1.835IleAsn: 1.835 ± 0.04	2.782IlePro: 2.782 ± 0.05	1.696IleGln: 1.696 ± 0.039	3.065IleArg: 3.065 ± 0.055	3.498IleSer: 3.498 ± 0.056	3.462IleThr: 3.462 ± 0.054	3.725IleVal: 3.725 ± 0.066	0.494IleTrp: 0.494 ± 0.021	1.802IleTyr: 1.802 ± 0.037	0.0IleXaa: 0.0 ± 0.0
Lys	4.238LysAla: 4.238 ± 0.055	0.531LysCys: 0.531 ± 0.021	2.443LysAsp: 2.443 ± 0.05	3.063LysGlu: 3.063 ± 0.067	1.259LysPhe: 1.259 ± 0.033	3.37LysGly: 3.37 ± 0.051	0.724LysHis: 0.724 ± 0.022	2.408LysIle: 2.408 ± 0.047	2.88LysLys: 2.88 ± 0.057	4.051LysLeu: 4.051 ± 0.058	1.253LysMet: 1.253 ± 0.038	1.888LysAsn: 1.888 ± 0.036	1.749LysPro: 1.749 ± 0.04	1.317LysGln: 1.317 ± 0.036	2.486LysArg: 2.486 ± 0.049	2.449LysSer: 2.449 ± 0.048	2.803LysThr: 2.803 ± 0.056	3.01LysVal: 3.01 ± 0.06	0.493LysTrp: 0.493 ± 0.022	1.697LysTyr: 1.697 ± 0.037	0.0LysXaa: 0.0 ± 0.0
Leu	9.651LeuAla: 9.651 ± 0.111	1.864LeuCys: 1.864 ± 0.043	5.643LeuAsp: 5.643 ± 0.072	6.281LeuGlu: 6.281 ± 0.092	3.851LeuPhe: 3.851 ± 0.07	7.407LeuGly: 7.407 ± 0.089	1.563LeuHis: 1.563 ± 0.032	4.73LeuIle: 4.73 ± 0.079	4.167LeuLys: 4.167 ± 0.061	10.198LeuLeu: 10.198 ± 0.152	2.495LeuMet: 2.495 ± 0.055	3.24LeuAsn: 3.24 ± 0.049	4.734LeuPro: 4.734 ± 0.059	2.519LeuGln: 2.519 ± 0.049	5.829LeuArg: 5.829 ± 0.09	7.026LeuSer: 7.026 ± 0.081	6.15LeuThr: 6.15 ± 0.078	6.19LeuVal: 6.19 ± 0.082	1.037LeuTrp: 1.037 ± 0.031	3.179LeuTyr: 3.179 ± 0.052	0.0LeuXaa: 0.0 ± 0.0
Met	2.665MetAla: 2.665 ± 0.055	0.305MetCys: 0.305 ± 0.016	1.828MetAsp: 1.828 ± 0.045	2.099MetGlu: 2.099 ± 0.04	0.787MetPhe: 0.787 ± 0.026	2.202MetGly: 2.202 ± 0.046	0.37MetHis: 0.37 ± 0.018	1.179MetIle: 1.179 ± 0.034	1.599MetLys: 1.599 ± 0.037	2.545MetLeu: 2.545 ± 0.053	0.69MetMet: 0.69 ± 0.023	1.137MetAsn: 1.137 ± 0.03	1.121MetPro: 1.121 ± 0.031	0.745MetGln: 0.745 ± 0.025	1.388MetArg: 1.388 ± 0.031	1.533MetSer: 1.533 ± 0.037	1.732MetThr: 1.732 ± 0.035	1.825MetVal: 1.825 ± 0.045	0.204MetTrp: 0.204 ± 0.013	0.653MetTyr: 0.653 ± 0.02	0.0MetXaa: 0.0 ± 0.0
Asn	3.186AsnAla: 3.186 ± 0.051	0.534AsnCys: 0.534 ± 0.02	1.651AsnAsp: 1.651 ± 0.04	1.795AsnGlu: 1.795 ± 0.041	1.222AsnPhe: 1.222 ± 0.036	3.092AsnGly: 3.092 ± 0.063	0.608AsnHis: 0.608 ± 0.021	2.185AsnIle: 2.185 ± 0.046	1.393AsnLys: 1.393 ± 0.033	3.101AsnLeu: 3.101 ± 0.051	0.901AsnMet: 0.901 ± 0.029	1.235AsnAsn: 1.235 ± 0.042	1.853AsnPro: 1.853 ± 0.042	1.043AsnGln: 1.043 ± 0.034	1.811AsnArg: 1.811 ± 0.039	1.735AsnSer: 1.735 ± 0.044	1.997AsnThr: 1.997 ± 0.052	2.264AsnVal: 2.264 ± 0.045	0.405AsnTrp: 0.405 ± 0.018	1.336AsnTyr: 1.336 ± 0.04	0.0AsnXaa: 0.0 ± 0.0
Pro	4.732ProAla: 4.732 ± 0.073	0.613ProCys: 0.613 ± 0.025	2.955ProAsp: 2.955 ± 0.056	3.525ProGlu: 3.525 ± 0.057	1.657ProPhe: 1.657 ± 0.038	3.899ProGly: 3.899 ± 0.061	0.7ProHis: 0.7 ± 0.023	2.027ProIle: 2.027 ± 0.042	1.536ProLys: 1.536 ± 0.04	3.565ProLeu: 3.565 ± 0.057	1.077ProMet: 1.077 ± 0.025	1.204ProAsn: 1.204 ± 0.033	1.603ProPro: 1.603 ± 0.049	1.336ProGln: 1.336 ± 0.039	1.939ProArg: 1.939 ± 0.046	2.284ProSer: 2.284 ± 0.05	2.157ProThr: 2.157 ± 0.05	3.423ProVal: 3.423 ± 0.057	0.504ProTrp: 0.504 ± 0.021	1.456ProTyr: 1.456 ± 0.039	0.0ProXaa: 0.0 ± 0.0
Gln	3.039GlnAla: 3.039 ± 0.052	0.373GlnCys: 0.373 ± 0.017	1.442GlnAsp: 1.442 ± 0.033	1.956GlnGlu: 1.956 ± 0.045	1.089GlnPhe: 1.089 ± 0.031	2.365GlnGly: 2.365 ± 0.062	0.454GlnHis: 0.454 ± 0.018	1.769GlnIle: 1.769 ± 0.04	1.583GlnLys: 1.583 ± 0.043	2.729GlnLeu: 2.729 ± 0.044	0.95GlnMet: 0.95 ± 0.028	1.258GlnAsn: 1.258 ± 0.034	1.157GlnPro: 1.157 ± 0.031	0.994GlnGln: 0.994 ± 0.032	1.765GlnArg: 1.765 ± 0.039	1.736GlnSer: 1.736 ± 0.038	1.698GlnThr: 1.698 ± 0.041	2.103GlnVal: 2.103 ± 0.038	0.335GlnTrp: 0.335 ± 0.016	1.214GlnTyr: 1.214 ± 0.036	0.0GlnXaa: 0.0 ± 0.0
Arg	5.639ArgAla: 5.639 ± 0.08	0.93ArgCys: 0.93 ± 0.032	3.182ArgAsp: 3.182 ± 0.061	4.626ArgGlu: 4.626 ± 0.074	2.298ArgPhe: 2.298 ± 0.045	4.068ArgGly: 4.068 ± 0.064	1.055ArgHis: 1.055 ± 0.027	3.006ArgIle: 3.006 ± 0.047	2.516ArgLys: 2.516 ± 0.048	5.795ArgLeu: 5.795 ± 0.08	1.712ArgMet: 1.712 ± 0.037	1.612ArgAsn: 1.612 ± 0.035	2.398ArgPro: 2.398 ± 0.042	2.062ArgGln: 2.062 ± 0.043	4.407ArgArg: 4.407 ± 0.085	2.956ArgSer: 2.956 ± 0.044	2.997ArgThr: 2.997 ± 0.051	3.905ArgVal: 3.905 ± 0.069	0.717ArgTrp: 0.717 ± 0.023	2.201ArgTyr: 2.201 ± 0.042	0.0ArgXaa: 0.0 ± 0.0
Ser	5.929SerAla: 5.929 ± 0.082	0.799SerCys: 0.799 ± 0.029	3.021SerAsp: 3.021 ± 0.051	2.963SerGlu: 2.963 ± 0.053	2.407SerPhe: 2.407 ± 0.039	5.871SerGly: 5.871 ± 0.084	0.995SerHis: 0.995 ± 0.03	3.133SerIle: 3.133 ± 0.051	2.131SerLys: 2.131 ± 0.048	5.259SerLeu: 5.259 ± 0.071	1.469SerMet: 1.469 ± 0.028	1.718SerAsn: 1.718 ± 0.051	2.436SerPro: 2.436 ± 0.049	1.63SerGln: 1.63 ± 0.036	3.199SerArg: 3.199 ± 0.054	3.103SerSer: 3.103 ± 0.057	2.89SerThr: 2.89 ± 0.058	4.341SerVal: 4.341 ± 0.07	0.635SerTrp: 0.635 ± 0.026	2.119SerTyr: 2.119 ± 0.045	0.0SerXaa: 0.0 ± 0.0
Thr	6.344ThrAla: 6.344 ± 0.107	0.709ThrCys: 0.709 ± 0.023	3.259ThrAsp: 3.259 ± 0.058	3.214ThrGlu: 3.214 ± 0.058	2.136ThrPhe: 2.136 ± 0.05	5.586ThrGly: 5.586 ± 0.096	0.935ThrHis: 0.935 ± 0.027	3.042ThrIle: 3.042 ± 0.058	1.927ThrLys: 1.927 ± 0.036	5.777ThrLeu: 5.777 ± 0.069	1.342ThrMet: 1.342 ± 0.029	1.716ThrAsn: 1.716 ± 0.042	2.935ThrPro: 2.935 ± 0.053	1.527ThrGln: 1.527 ± 0.035	2.712ThrArg: 2.712 ± 0.046	2.622ThrSer: 2.622 ± 0.054	2.819ThrThr: 2.819 ± 0.057	5.509ThrVal: 5.509 ± 0.102	0.683ThrTrp: 0.683 ± 0.027	2.0ThrTyr: 2.0 ± 0.056	0.0ThrXaa: 0.0 ± 0.0
Val	6.172ValAla: 6.172 ± 0.087	1.372ValCys: 1.372 ± 0.031	4.337ValAsp: 4.337 ± 0.066	4.768ValGlu: 4.768 ± 0.052	2.986ValPhe: 2.986 ± 0.055	5.212ValGly: 5.212 ± 0.067	1.107ValHis: 1.107 ± 0.035	3.944ValIle: 3.944 ± 0.064	3.411ValLys: 3.411 ± 0.052	7.499ValLeu: 7.499 ± 0.081	1.929ValMet: 1.929 ± 0.04	2.608ValAsn: 2.608 ± 0.047	3.229ValPro: 3.229 ± 0.056	1.906ValGln: 1.906 ± 0.038	4.211ValArg: 4.211 ± 0.055	4.875ValSer: 4.875 ± 0.063	4.983ValThr: 4.983 ± 0.101	5.397ValVal: 5.397 ± 0.083	0.8ValTrp: 0.8 ± 0.029	2.472ValTyr: 2.472 ± 0.042	0.0ValXaa: 0.0 ± 0.0
Trp	1.086TrpAla: 1.086 ± 0.036	0.197TrpCys: 0.197 ± 0.013	0.699TrpAsp: 0.699 ± 0.027	0.743TrpGlu: 0.743 ± 0.025	0.475TrpPhe: 0.475 ± 0.022	0.862TrpGly: 0.862 ± 0.027	0.204TrpHis: 0.204 ± 0.015	0.485TrpIle: 0.485 ± 0.02	0.489TrpLys: 0.489 ± 0.021	1.103TrpLeu: 1.103 ± 0.03	0.299TrpMet: 0.299 ± 0.016	0.467TrpAsn: 0.467 ± 0.018	0.343TrpPro: 0.343 ± 0.019	0.394TrpGln: 0.394 ± 0.018	0.653TrpArg: 0.653 ± 0.025	0.712TrpSer: 0.712 ± 0.025	0.67TrpThr: 0.67 ± 0.031	0.74TrpVal: 0.74 ± 0.024	0.125TrpTrp: 0.125 ± 0.011	0.53TrpTyr: 0.53 ± 0.02	0.0TrpXaa: 0.0 ± 0.0
Tyr	3.16TyrAla: 3.16 ± 0.05	0.578TyrCys: 0.578 ± 0.022	2.304TyrAsp: 2.304 ± 0.043	2.248TyrGlu: 2.248 ± 0.04	1.527TyrPhe: 1.527 ± 0.039	2.956TyrGly: 2.956 ± 0.048	0.723TyrHis: 0.723 ± 0.023	1.983TyrIle: 1.983 ± 0.044	1.311TyrLys: 1.311 ± 0.039	3.514TyrLeu: 3.514 ± 0.048	0.747TyrMet: 0.747 ± 0.022	1.322TyrAsn: 1.322 ± 0.033	1.512TyrPro: 1.512 ± 0.033	1.245TyrGln: 1.245 ± 0.031	2.219TyrArg: 2.219 ± 0.047	2.0TyrSer: 2.0 ± 0.039	2.342TyrThr: 2.342 ± 0.064	2.216TyrVal: 2.216 ± 0.041	0.402TyrTrp: 0.402 ± 0.018	1.557TyrTyr: 1.557 ± 0.041	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4105 proteins (1302255 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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