Caulobacter virus Rogue
Average proteome isoelectric point is 6.39
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 331 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|K4JNF0|K4JNF0_9CAUD Putative transglutaminase-like cysteine peptidase OS=Caulobacter virus Rogue OX=1211643 GN=CcrRogue_gp044 PE=4 SV=1
MM1 pKa = 7.52 PHH3 pKa = 7.29 LSKK6 pKa = 10.7 QSYY9 pKa = 9.69 SFDD12 pKa = 3.7 YY13 pKa = 11.0 TSDD16 pKa = 3.19 ALAAAGEE23 pKa = 4.45 FEE25 pKa = 4.45 ALTTEE30 pKa = 5.19 FYY32 pKa = 10.42 RR33 pKa = 11.84 YY34 pKa = 5.92 EE35 pKa = 3.81 TRR37 pKa = 11.84 YY38 pKa = 10.58 LYY40 pKa = 10.69 DD41 pKa = 3.32 AARR44 pKa = 11.84 AIVGVEE50 pKa = 3.81 VRR52 pKa = 11.84 VMDD55 pKa = 3.73 EE56 pKa = 4.49 DD57 pKa = 4.41 GFFVGYY63 pKa = 9.65 VAGEE67 pKa = 3.86 AA68 pKa = 3.67
Molecular weight: 7.66 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.053
IPC2_protein 4.355
IPC_protein 4.19
Toseland 4.024
ProMoST 4.317
Dawson 4.151
Bjellqvist 4.304
Wikipedia 4.037
Rodwell 4.037
Grimsley 3.948
Solomon 4.139
Lehninger 4.088
Nozaki 4.266
DTASelect 4.406
Thurlkill 4.062
EMBOSS 4.05
Sillero 4.304
Patrickios 0.846
IPC_peptide 4.139
IPC2_peptide 4.291
IPC2.peptide.svr19 4.207
Protein with the highest isoelectric point:
>tr|K4JS17|K4JS17_9CAUD Uncharacterized protein OS=Caulobacter virus Rogue OX=1211643 GN=CcrRogue_gp041 PE=4 SV=1
MM1 pKa = 7.64 KK2 pKa = 10.25 RR3 pKa = 11.84 AQCKK7 pKa = 9.98 AGRR10 pKa = 11.84 LLLGWTQAEE19 pKa = 4.19 LADD22 pKa = 3.87 AAGCAVSTVADD33 pKa = 3.75 YY34 pKa = 11.34 EE35 pKa = 4.2 RR36 pKa = 11.84 GARR39 pKa = 11.84 RR40 pKa = 11.84 TDD42 pKa = 3.02 EE43 pKa = 4.21 SIIRR47 pKa = 11.84 RR48 pKa = 11.84 FVDD51 pKa = 3.16 SFSRR55 pKa = 11.84 RR56 pKa = 11.84 GVRR59 pKa = 11.84 FTRR62 pKa = 11.84 TSVIHH67 pKa = 6.45 PEE69 pKa = 4.6 FKK71 pKa = 10.78 AA72 pKa = 3.42
Molecular weight: 8.09 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.345
IPC2_protein 9.37
IPC_protein 10.101
Toseland 10.57
ProMoST 10.262
Dawson 10.657
Bjellqvist 10.379
Wikipedia 10.862
Rodwell 10.76
Grimsley 10.687
Solomon 10.804
Lehninger 10.774
Nozaki 10.599
DTASelect 10.365
Thurlkill 10.57
EMBOSS 10.979
Sillero 10.599
Patrickios 10.599
IPC_peptide 10.818
IPC2_peptide 9.633
IPC2.peptide.svr19 8.546
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
331
0
331
65181
27
2805
196.9
21.66
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.14 ± 0.209
0.936 ± 0.063
6.692 ± 0.195
6.137 ± 0.254
3.756 ± 0.099
8.4 ± 0.635
2.106 ± 0.098
4.692 ± 0.103
4.692 ± 0.193
8.088 ± 0.167
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.396 ± 0.11
2.982 ± 0.115
5.152 ± 0.134
3.248 ± 0.106
6.608 ± 0.273
5.192 ± 0.188
6.157 ± 0.298
6.918 ± 0.117
1.683 ± 0.067
3.027 ± 0.092
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here