Agarivorans albus MKT 106
Average proteome isoelectric point is 6.12
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4397 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|R9PPL1|R9PPL1_AGAAL Putrescine utilization regulator OS=Agarivorans albus MKT 106 OX=1331007 GN=AALB_0141 PE=4 SV=1
MM1 pKa = 7.62 RR2 pKa = 11.84 LSRR5 pKa = 11.84 IATAVTLFASVTIAGCGSSNSPFDD29 pKa = 3.98 FGNDD33 pKa = 2.97 ITPNEE38 pKa = 4.11 PNSVKK43 pKa = 10.08 IRR45 pKa = 11.84 FNPLTGDD52 pKa = 3.06 VSTPNNLLFNGSLDD66 pKa = 3.53 GTINLPSEE74 pKa = 3.86 AAIALTQQYY83 pKa = 10.78 QDD85 pKa = 2.99 ITNVLGALDD94 pKa = 4.39 GWQTSVPFSIPLAYY108 pKa = 10.34 GDD110 pKa = 5.64 DD111 pKa = 3.43 DD112 pKa = 4.8 AEE114 pKa = 4.29 YY115 pKa = 11.16 NEE117 pKa = 5.19 EE118 pKa = 4.51 IDD120 pKa = 3.87 TKK122 pKa = 11.15 LDD124 pKa = 3.45 ASSFPAGVLLYY135 pKa = 10.11 KK136 pKa = 10.47 VRR138 pKa = 11.84 ASGFADD144 pKa = 3.58 TCADD148 pKa = 3.32 VTEE151 pKa = 4.67 NGGSFVAGQTCRR163 pKa = 11.84 VDD165 pKa = 3.25 EE166 pKa = 3.96 QLVYY170 pKa = 11.08 GEE172 pKa = 5.35 DD173 pKa = 4.05 YY174 pKa = 10.85 IATFSDD180 pKa = 3.58 GSLIVTPLVPFAPATSYY197 pKa = 10.17 MVAITDD203 pKa = 3.82 QLLDD207 pKa = 3.6 EE208 pKa = 4.95 NGRR211 pKa = 11.84 GVAAGEE217 pKa = 4.25 AYY219 pKa = 10.11 QLLSSPITADD229 pKa = 3.9 DD230 pKa = 4.27 DD231 pKa = 3.98 ATVQSLKK238 pKa = 11.07 GLTAFNNGLLAAEE251 pKa = 4.33 GVTANVSYY259 pKa = 10.89 SAVFTTQSVGSVLTTLQQIYY279 pKa = 10.82 GGMALNSLLPLSALVDD295 pKa = 3.76 TGTTAQDD302 pKa = 3.27 VANAALGSSQPGFVAAKK319 pKa = 9.48 YY320 pKa = 9.38 FKK322 pKa = 9.98 STLAVPYY329 pKa = 10.14 YY330 pKa = 10.8 LSDD333 pKa = 4.29 LDD335 pKa = 5.09 NPDD338 pKa = 3.1 LTGADD343 pKa = 3.54 VNKK346 pKa = 9.29 WAQAKK351 pKa = 8.61 TDD353 pKa = 3.66 SPVAILQLLQTNADD367 pKa = 3.74 FADD370 pKa = 3.64 QGSATSFWAQAGAKK384 pKa = 9.29 GFDD387 pKa = 3.18 VATFGGLVAGGDD399 pKa = 3.76 TTTAGQMLAQADD411 pKa = 4.38 LAGLTYY417 pKa = 9.81 MQDD420 pKa = 3.49 GEE422 pKa = 4.35 AMPVDD427 pKa = 3.77 SHH429 pKa = 6.79 RR430 pKa = 11.84 HH431 pKa = 3.64 LTAYY435 pKa = 10.72 NPVPEE440 pKa = 4.5 TKK442 pKa = 9.17 STAFLDD448 pKa = 3.61 LDD450 pKa = 3.75 IYY452 pKa = 11.31 LPDD455 pKa = 3.43 EE456 pKa = 4.37 TLTGNTMPAEE466 pKa = 4.49 GWPVVIFQHH475 pKa = 7.08 GIGSVKK481 pKa = 8.69 EE482 pKa = 3.79 TAAAIAASYY491 pKa = 9.21 AAQGYY496 pKa = 9.62 AVLAMDD502 pKa = 4.93 LPYY505 pKa = 10.58 HH506 pKa = 6.63 GSRR509 pKa = 11.84 GLDD512 pKa = 3.12 LTGDD516 pKa = 3.88 GNAEE520 pKa = 3.54 ISANDD525 pKa = 3.81 DD526 pKa = 3.42 PQVFANLSSFLSIRR540 pKa = 11.84 EE541 pKa = 3.88 NLRR544 pKa = 11.84 QSTADD549 pKa = 3.73 LLAVRR554 pKa = 11.84 AALQAAGAPAEE565 pKa = 4.01 LDD567 pKa = 3.41 GSKK570 pKa = 9.84 VHH572 pKa = 6.53 YY573 pKa = 10.35 AGQSLGAIVGVGPASVADD591 pKa = 3.88 TVNVSGFDD599 pKa = 3.08 IDD601 pKa = 4.63 YY602 pKa = 10.56 SLQSVSLSAPAGGLAGVFQYY622 pKa = 10.86 SPSFGPIVEE631 pKa = 5.23 AGLKK635 pKa = 10.37 SNPGYY640 pKa = 10.29 LALVAAGLGFEE651 pKa = 5.53 DD652 pKa = 5.15 NDD654 pKa = 3.79 TATALEE660 pKa = 4.13 QFAVYY665 pKa = 9.75 QATYY669 pKa = 10.13 PEE671 pKa = 4.37 AAQLVIDD678 pKa = 3.99 SNYY681 pKa = 9.75 PAFASIFGSLAQTVVDD697 pKa = 5.05 GVDD700 pKa = 3.97 PINYY704 pKa = 8.05 AQSGFAMPVLVHH716 pKa = 6.63 EE717 pKa = 4.53 IVGDD721 pKa = 3.87 GSEE724 pKa = 4.29 GSGDD728 pKa = 3.51 QVIPNSLATLPLVGTEE744 pKa = 4.01 PLIASMGLEE753 pKa = 4.09 GTEE756 pKa = 3.99 VTSAGSYY763 pKa = 10.1 AVRR766 pKa = 11.84 FSQGSHH772 pKa = 6.06 SSLISPAASLAATVEE787 pKa = 4.19 MQTQFVSYY795 pKa = 10.24 AVSADD800 pKa = 3.21 AGNASVVVSDD810 pKa = 4.28 SSVIAPAQQ818 pKa = 3.24
Molecular weight: 84.28 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.719
IPC2_protein 3.757
IPC_protein 3.808
Toseland 3.579
ProMoST 3.961
Dawson 3.795
Bjellqvist 3.948
Wikipedia 3.732
Rodwell 3.617
Grimsley 3.478
Solomon 3.795
Lehninger 3.757
Nozaki 3.91
DTASelect 4.177
Thurlkill 3.617
EMBOSS 3.745
Sillero 3.923
Patrickios 0.744
IPC_peptide 3.795
IPC2_peptide 3.897
IPC2.peptide.svr19 3.822
Protein with the highest isoelectric point:
>tr|R9PGZ8|R9PGZ8_AGAAL LemA protein OS=Agarivorans albus MKT 106 OX=1331007 GN=AALB_0697 PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVLKK11 pKa = 10.42 RR12 pKa = 11.84 KK13 pKa = 9.36 RR14 pKa = 11.84 SHH16 pKa = 6.15 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.4 NGRR28 pKa = 11.84 QVLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.5 GRR39 pKa = 11.84 ASLSAA44 pKa = 3.83
Molecular weight: 5.07 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.199
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.647
Grimsley 12.998
Solomon 13.451
Lehninger 13.364
Nozaki 12.954
DTASelect 12.954
Thurlkill 12.954
EMBOSS 13.466
Sillero 12.954
Patrickios 12.369
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.154
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4397
0
4397
1381857
37
4824
314.3
34.84
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.308 ± 0.045
1.035 ± 0.015
5.325 ± 0.037
6.11 ± 0.035
4.096 ± 0.025
6.718 ± 0.036
2.181 ± 0.019
5.838 ± 0.028
4.937 ± 0.035
10.914 ± 0.052
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.401 ± 0.017
4.292 ± 0.028
3.873 ± 0.021
5.607 ± 0.04
4.275 ± 0.028
7.01 ± 0.039
4.722 ± 0.031
6.905 ± 0.029
1.354 ± 0.018
3.1 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here