Oribacterium parvum ACB1
Average proteome isoelectric point is 6.21
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2127 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|G9WLA7|G9WLA7_9FIRM Phospho-N-acetylmuramoyl-pentapeptide-transferase OS=Oribacterium parvum ACB1 OX=796943 GN=mraY PE=3 SV=1
MM1 pKa = 6.69 EE2 pKa = 5.06 TKK4 pKa = 10.51 HH5 pKa = 7.7 SDD7 pKa = 3.05 EE8 pKa = 4.2 FLSNKK13 pKa = 8.89 EE14 pKa = 3.9 WLQTALSSEE23 pKa = 4.19 KK24 pKa = 10.74 VILRR28 pKa = 11.84 GISALEE34 pKa = 3.73 YY35 pKa = 9.82 LQLFPGYY42 pKa = 9.48 IGEE45 pKa = 4.46 KK46 pKa = 9.9 NIEE49 pKa = 4.24 VYY51 pKa = 10.86 SLTEE55 pKa = 3.83 GQYY58 pKa = 11.63 SNIQYY63 pKa = 10.57 SIVNSFDD70 pKa = 3.61 GIEE73 pKa = 3.97 YY74 pKa = 10.35 LDD76 pKa = 4.94 DD77 pKa = 5.75 GIVLCSTLEE86 pKa = 3.81 QTIKK90 pKa = 11.12 DD91 pKa = 4.53 FIRR94 pKa = 11.84 DD95 pKa = 3.43 YY96 pKa = 10.39 DD97 pKa = 3.76 TSDD100 pKa = 2.86 THH102 pKa = 7.82 VLVEE106 pKa = 4.05 ALGNYY111 pKa = 10.19 YY112 pKa = 10.6 YY113 pKa = 11.0 FNNFTFDD120 pKa = 3.52 KK121 pKa = 10.85 LIVDD125 pKa = 4.28 TDD127 pKa = 3.44 QVLFDD132 pKa = 4.74 EE133 pKa = 4.71 VKK135 pKa = 8.0 EE136 pKa = 3.91 WAIGFVQGANYY147 pKa = 10.0 DD148 pKa = 3.43
Molecular weight: 17.09 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.841
IPC2_protein 4.05
IPC_protein 3.999
Toseland 3.808
ProMoST 4.088
Dawson 3.973
Bjellqvist 4.151
Wikipedia 3.872
Rodwell 3.821
Grimsley 3.719
Solomon 3.961
Lehninger 3.91
Nozaki 4.088
DTASelect 4.266
Thurlkill 3.846
EMBOSS 3.884
Sillero 4.113
Patrickios 0.769
IPC_peptide 3.961
IPC2_peptide 4.088
IPC2.peptide.svr19 4.018
Protein with the highest isoelectric point:
>tr|S2LMD7|S2LMD7_9FIRM Uncharacterized protein (Fragment) OS=Oribacterium parvum ACB1 OX=796943 GN=HMPREF9625_02204 PE=4 SV=1
MM1 pKa = 7.27 FFVSKK6 pKa = 9.42 LTEE9 pKa = 3.93 HH10 pKa = 7.02 LRR12 pKa = 11.84 LLCLGKK18 pKa = 10.6 YY19 pKa = 9.53 KK20 pKa = 10.63 KK21 pKa = 10.71 SPAKK25 pKa = 10.06 KK26 pKa = 9.73 VSLPRR31 pKa = 11.84 TNKK34 pKa = 9.37 QIIYY38 pKa = 8.39 PRR40 pKa = 11.84 CHH42 pKa = 7.44 LDD44 pKa = 3.01 SQLTLRR50 pKa = 11.84 RR51 pKa = 11.84 LAGYY55 pKa = 7.26 QHH57 pKa = 6.85 IPGNSRR63 pKa = 11.84 IPTRR67 pKa = 11.84 CRR69 pKa = 11.84 ILRR72 pKa = 11.84 KK73 pKa = 10.09 NPFPCTLSGPFDD85 pKa = 3.7 NLFLTRR91 pKa = 11.84 FSASRR96 pKa = 11.84 VLCKK100 pKa = 10.68 GIVAVISTSTVYY112 pKa = 10.74 NIQLLRR118 pKa = 11.84 TDD120 pKa = 4.34 FTRR123 pKa = 11.84 KK124 pKa = 9.75 YY125 pKa = 10.3 KK126 pKa = 10.48 SCQAKK131 pKa = 9.48 KK132 pKa = 10.52 SYY134 pKa = 10.35 GITIICIFLSDD145 pKa = 4.23 RR146 pKa = 11.84 LVLPPFQTVRR156 pKa = 11.84 TTVQMEE162 pKa = 3.86 YY163 pKa = 11.02 SPFF166 pKa = 3.72
Molecular weight: 19.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.334
IPC2_protein 9.78
IPC_protein 10.262
Toseland 10.555
ProMoST 10.189
Dawson 10.672
Bjellqvist 10.365
Wikipedia 10.847
Rodwell 11.082
Grimsley 10.73
Solomon 10.73
Lehninger 10.701
Nozaki 10.57
DTASelect 10.35
Thurlkill 10.555
EMBOSS 10.935
Sillero 10.599
Patrickios 10.774
IPC_peptide 10.73
IPC2_peptide 9.487
IPC2.peptide.svr19 8.502
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2127
0
2127
714622
29
2899
336.0
37.83
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.989 ± 0.051
1.189 ± 0.022
5.132 ± 0.042
8.697 ± 0.068
4.736 ± 0.043
7.044 ± 0.048
1.715 ± 0.021
7.096 ± 0.049
7.768 ± 0.043
9.935 ± 0.058
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.807 ± 0.024
4.204 ± 0.035
3.233 ± 0.027
2.928 ± 0.027
4.412 ± 0.037
6.647 ± 0.044
4.577 ± 0.038
6.212 ± 0.037
0.792 ± 0.016
3.888 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here