Serpentinomonas raichei
Average proteome isoelectric point is 6.95
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2363 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A060NT44|A0A060NT44_9BURK Thioredoxin domain-containing protein OS=Serpentinomonas raichei OX=1458425 GN=SRAA_2225 PE=3 SV=1
MM1 pKa = 7.64 AATTLIMKK9 pKa = 7.59 TFTAIVEE16 pKa = 4.47 KK17 pKa = 10.17 CTEE20 pKa = 3.73 TGLYY24 pKa = 9.0 VGYY27 pKa = 10.94 VPGFPGAHH35 pKa = 5.05 TQGTSLDD42 pKa = 3.82 EE43 pKa = 4.23 LQQNLQEE50 pKa = 4.33 VVSMLLEE57 pKa = 4.91 DD58 pKa = 4.57 GEE60 pKa = 4.75 PVLDD64 pKa = 3.77 AQFVGTQQLAIAA76 pKa = 4.89
Molecular weight: 8.13 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.858
IPC2_protein 4.139
IPC_protein 3.923
Toseland 3.77
ProMoST 4.075
Dawson 3.884
Bjellqvist 4.05
Wikipedia 3.795
Rodwell 3.77
Grimsley 3.706
Solomon 3.859
Lehninger 3.808
Nozaki 4.037
DTASelect 4.113
Thurlkill 3.821
EMBOSS 3.808
Sillero 4.037
Patrickios 1.901
IPC_peptide 3.859
IPC2_peptide 4.024
IPC2.peptide.svr19 3.949
Protein with the highest isoelectric point:
>tr|A0A060NF26|A0A060NF26_9BURK Beta sliding clamp OS=Serpentinomonas raichei OX=1458425 GN=SRAA_0002 PE=3 SV=1
MM1 pKa = 7.46 EE2 pKa = 5.58 PALNHH7 pKa = 6.89 ANLTLSGRR15 pKa = 11.84 EE16 pKa = 3.93 LARR19 pKa = 11.84 MRR21 pKa = 11.84 RR22 pKa = 11.84 LAMATEE28 pKa = 4.51 GKK30 pKa = 10.19 VGAANRR36 pKa = 11.84 VAAAVRR42 pKa = 11.84 ASQAAAQAVQAAARR56 pKa = 11.84 RR57 pKa = 11.84 DD58 pKa = 3.66 EE59 pKa = 4.35 AQCGCSGAADD69 pKa = 4.13 AACQCDD75 pKa = 3.52 VAAAVSAPASQTKK88 pKa = 9.35 AACALPNGRR97 pKa = 11.84 TLARR101 pKa = 11.84 ARR103 pKa = 11.84 RR104 pKa = 11.84 QTLAQEE110 pKa = 4.53 GKK112 pKa = 10.69 VGIQKK117 pKa = 9.54 VANASRR123 pKa = 11.84 IAATMPDD130 pKa = 3.24 RR131 pKa = 11.84 ADD133 pKa = 3.07 WQTALVQGVTGRR145 pKa = 11.84 QLAMQKK151 pKa = 10.4 RR152 pKa = 11.84 IVQSLAGRR160 pKa = 11.84 TEE162 pKa = 4.1 AQHH165 pKa = 6.45 SSGNRR170 pKa = 11.84 SVARR174 pKa = 11.84 SRR176 pKa = 11.84 SRR178 pKa = 11.84 PVAEE182 pKa = 3.88 RR183 pKa = 11.84 TEE185 pKa = 4.21 VGHH188 pKa = 5.62 TLSGQEE194 pKa = 3.56 VSGIQVEE201 pKa = 4.47 RR202 pKa = 11.84 SSKK205 pKa = 9.22 VTGQEE210 pKa = 3.5 PGTCRR215 pKa = 11.84 NVTGTEE221 pKa = 4.18 YY222 pKa = 10.18 IGMEE226 pKa = 4.48 QYY228 pKa = 10.41 QAWCEE233 pKa = 4.31 SKK235 pKa = 10.34 PQPRR239 pKa = 11.84 PPKK242 pKa = 10.14 VGLSQTTGQQQSISGTEE259 pKa = 3.77 VDD261 pKa = 3.69 PQPRR265 pKa = 11.84 VTGNQTGVCLGITGTQYY282 pKa = 11.47 LSDD285 pKa = 3.79 LTTRR289 pKa = 11.84 LCQEE293 pKa = 4.0 QPLQGPHH300 pKa = 6.33 KK301 pKa = 9.57 VSVMSSRR308 pKa = 11.84 GQQTVTGVVVNGGSKK323 pKa = 8.51 VTGNEE328 pKa = 3.52 AGMQRR333 pKa = 11.84 PITGTQYY340 pKa = 11.52 ARR342 pKa = 11.84 ALQAAPSRR350 pKa = 11.84 MPASEE355 pKa = 4.21 ARR357 pKa = 11.84 HH358 pKa = 5.71 ALPEE362 pKa = 4.01 PVQVQRR368 pKa = 11.84 QSASWRR374 pKa = 11.84 TQSLTGDD381 pKa = 3.43 RR382 pKa = 11.84 PGIGGGGVTGDD393 pKa = 3.42 EE394 pKa = 4.48 RR395 pKa = 11.84 GACEE399 pKa = 5.31 PITGTPFIGPDD410 pKa = 3.27 NQYY413 pKa = 10.9 AACTIDD419 pKa = 3.95 SAWLTRR425 pKa = 11.84 HH426 pKa = 6.5 PEE428 pKa = 4.07 LAVEE432 pKa = 4.72 TPPAAPKK439 pKa = 10.2 GFSIARR445 pKa = 11.84 PQRR448 pKa = 11.84 GQPAGRR454 pKa = 11.84 ATTPQSAAPRR464 pKa = 11.84 NQVTGVAYY472 pKa = 10.33 GNSEE476 pKa = 4.9 RR477 pKa = 11.84 ITGPGFKK484 pKa = 10.19 AQGVITGTPEE494 pKa = 3.56 FRR496 pKa = 11.84 HH497 pKa = 6.23 GGQSLDD503 pKa = 3.45 SRR505 pKa = 11.84 RR506 pKa = 11.84 SSARR510 pKa = 11.84 PATPASPPAVQPPASEE526 pKa = 4.1 RR527 pKa = 11.84 LSGEE531 pKa = 3.59 GRR533 pKa = 11.84 QQGSRR538 pKa = 11.84 LTGDD542 pKa = 2.8 SWSSSRR548 pKa = 11.84 HH549 pKa = 3.99 ISGTDD554 pKa = 3.34 GVSAQSRR561 pKa = 11.84 NPTQRR566 pKa = 11.84 GQPRR570 pKa = 11.84 AMGMGMDD577 pKa = 3.45 ARR579 pKa = 11.84 SVRR582 pKa = 11.84 EE583 pKa = 3.69 QARR586 pKa = 11.84 AEE588 pKa = 4.24 VPPSPVTGSSGNTGRR603 pKa = 11.84 GAMVTISGGARR614 pKa = 11.84 GG615 pKa = 3.54
Molecular weight: 64.02 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.354
IPC2_protein 9.385
IPC_protein 10.277
Toseland 10.54
ProMoST 10.321
Dawson 10.643
Bjellqvist 10.409
Wikipedia 10.877
Rodwell 10.687
Grimsley 10.687
Solomon 10.804
Lehninger 10.76
Nozaki 10.584
DTASelect 10.394
Thurlkill 10.555
EMBOSS 10.965
Sillero 10.599
Patrickios 10.365
IPC_peptide 10.804
IPC2_peptide 9.765
IPC2.peptide.svr19 8.588
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2363
0
2363
785481
41
4077
332.4
36.13
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.42 ± 0.076
1.016 ± 0.019
4.712 ± 0.041
5.363 ± 0.042
3.264 ± 0.028
8.054 ± 0.045
2.563 ± 0.031
3.997 ± 0.042
2.527 ± 0.049
12.183 ± 0.081
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.127 ± 0.025
2.33 ± 0.035
5.715 ± 0.04
5.255 ± 0.049
7.26 ± 0.048
5.025 ± 0.034
4.573 ± 0.044
7.049 ± 0.047
1.586 ± 0.025
1.98 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here