Amino acid dipepetide frequency for Ruminococcus flavefaciens

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	8.92AlaAla: 8.92 ± 0.135	1.05AlaCys: 1.05 ± 0.03	5.679AlaAsp: 5.679 ± 0.076	6.761AlaGlu: 6.761 ± 0.123	3.306AlaPhe: 3.306 ± 0.075	5.25AlaGly: 5.25 ± 0.086	1.099AlaHis: 1.099 ± 0.03	4.772AlaIle: 4.772 ± 0.076	5.105AlaLys: 5.105 ± 0.082	6.667AlaLeu: 6.667 ± 0.097	2.237AlaMet: 2.237 ± 0.052	2.998AlaAsn: 2.998 ± 0.066	2.689AlaPro: 2.689 ± 0.071	2.146AlaGln: 2.146 ± 0.056	2.413AlaArg: 2.413 ± 0.055	4.205AlaSer: 4.205 ± 0.074	3.139AlaThr: 3.139 ± 0.075	7.176AlaVal: 7.176 ± 0.11	0.551AlaTrp: 0.551 ± 0.026	2.774AlaTyr: 2.774 ± 0.05	0.0AlaXaa: 0.0 ± 0.0
Cys	1.273CysAla: 1.273 ± 0.04	0.354CysCys: 0.354 ± 0.019	1.114CysAsp: 1.114 ± 0.035	1.084CysGlu: 1.084 ± 0.034	0.759CysPhe: 0.759 ± 0.029	1.856CysGly: 1.856 ± 0.049	0.295CysHis: 0.295 ± 0.018	1.187CysIle: 1.187 ± 0.036	0.913CysLys: 0.913 ± 0.035	1.106CysLeu: 1.106 ± 0.038	0.381CysMet: 0.381 ± 0.018	0.664CysAsn: 0.664 ± 0.027	0.638CysPro: 0.638 ± 0.028	0.325CysGln: 0.325 ± 0.018	0.696CysArg: 0.696 ± 0.028	1.129CysSer: 1.129 ± 0.038	0.943CysThr: 0.943 ± 0.036	0.972CysVal: 0.972 ± 0.036	0.118CysTrp: 0.118 ± 0.011	0.628CysTyr: 0.628 ± 0.028	0.0CysXaa: 0.0 ± 0.0
Asp	4.003AspAla: 4.003 ± 0.073	0.974AspCys: 0.974 ± 0.032	4.084AspAsp: 4.084 ± 0.078	5.164AspGlu: 5.164 ± 0.076	3.128AspPhe: 3.128 ± 0.061	5.092AspGly: 5.092 ± 0.104	0.914AspHis: 0.914 ± 0.036	5.882AspIle: 5.882 ± 0.084	4.948AspLys: 4.948 ± 0.078	4.197AspLeu: 4.197 ± 0.072	2.154AspMet: 2.154 ± 0.046	3.09AspAsn: 3.09 ± 0.06	1.76AspPro: 1.76 ± 0.052	0.982AspGln: 0.982 ± 0.035	2.396AspArg: 2.396 ± 0.046	4.035AspSer: 4.035 ± 0.064	3.586AspThr: 3.586 ± 0.056	4.017AspVal: 4.017 ± 0.065	0.614AspTrp: 0.614 ± 0.029	3.177AspTyr: 3.177 ± 0.062	0.0AspXaa: 0.0 ± 0.0
Glu	5.249GluAla: 5.249 ± 0.074	0.934GluCys: 0.934 ± 0.032	3.713GluAsp: 3.713 ± 0.061	5.277GluGlu: 5.277 ± 0.092	2.731GluPhe: 2.731 ± 0.06	4.017GluGly: 4.017 ± 0.071	1.247GluHis: 1.247 ± 0.034	4.984GluIle: 4.984 ± 0.066	6.363GluLys: 6.363 ± 0.094	7.096GluLeu: 7.096 ± 0.107	2.107GluMet: 2.107 ± 0.049	4.273GluAsn: 4.273 ± 0.069	2.029GluPro: 2.029 ± 0.048	2.279GluGln: 2.279 ± 0.053	3.054GluArg: 3.054 ± 0.068	3.573GluSer: 3.573 ± 0.065	3.624GluThr: 3.624 ± 0.06	3.755GluVal: 3.755 ± 0.066	0.687GluTrp: 0.687 ± 0.029	3.339GluTyr: 3.339 ± 0.058	0.0GluXaa: 0.0 ± 0.0
Phe	3.278PheAla: 3.278 ± 0.064	0.796PheCys: 0.796 ± 0.027	3.172PheAsp: 3.172 ± 0.064	2.844PheGlu: 2.844 ± 0.056	1.907PhePhe: 1.907 ± 0.055	3.382PheGly: 3.382 ± 0.07	0.625PheHis: 0.625 ± 0.026	3.342PheIle: 3.342 ± 0.071	2.451PheLys: 2.451 ± 0.053	3.2PheLeu: 3.2 ± 0.068	1.214PheMet: 1.214 ± 0.038	1.892PheAsn: 1.892 ± 0.046	1.291PhePro: 1.291 ± 0.041	0.755PheGln: 0.755 ± 0.028	2.0PheArg: 2.0 ± 0.049	3.37PheSer: 3.37 ± 0.075	2.737PheThr: 2.737 ± 0.05	2.681PheVal: 2.681 ± 0.053	0.378PheTrp: 0.378 ± 0.022	1.759PheTyr: 1.759 ± 0.044	0.0PheXaa: 0.0 ± 0.0
Gly	5.196GlyAla: 5.196 ± 0.079	1.338GlyCys: 1.338 ± 0.047	4.524GlyAsp: 4.524 ± 0.077	4.685GlyGlu: 4.685 ± 0.078	3.311GlyPhe: 3.311 ± 0.069	5.548GlyGly: 5.548 ± 0.123	1.14GlyHis: 1.14 ± 0.037	5.495GlyIle: 5.495 ± 0.093	5.445GlyLys: 5.445 ± 0.079	5.183GlyLeu: 5.183 ± 0.081	2.046GlyMet: 2.046 ± 0.051	3.256GlyAsn: 3.256 ± 0.089	0.997GlyPro: 0.997 ± 0.032	1.772GlyGln: 1.772 ± 0.043	2.923GlyArg: 2.923 ± 0.065	4.737GlySer: 4.737 ± 0.089	4.37GlyThr: 4.37 ± 0.111	4.7GlyVal: 4.7 ± 0.077	0.731GlyTrp: 0.731 ± 0.034	3.24GlyTyr: 3.24 ± 0.057	0.0GlyXaa: 0.0 ± 0.0
His	0.95HisAla: 0.95 ± 0.031	0.324HisCys: 0.324 ± 0.019	1.042HisAsp: 1.042 ± 0.035	1.032HisGlu: 1.032 ± 0.031	0.823HisPhe: 0.823 ± 0.032	1.211HisGly: 1.211 ± 0.04	0.348HisHis: 0.348 ± 0.026	1.429HisIle: 1.429 ± 0.039	0.959HisLys: 0.959 ± 0.03	1.192HisLeu: 1.192 ± 0.035	0.413HisMet: 0.413 ± 0.02	0.766HisAsn: 0.766 ± 0.03	0.595HisPro: 0.595 ± 0.027	0.352HisGln: 0.352 ± 0.02	0.728HisArg: 0.728 ± 0.027	1.128HisSer: 1.128 ± 0.035	0.912HisThr: 0.912 ± 0.027	0.791HisVal: 0.791 ± 0.03	0.141HisTrp: 0.141 ± 0.012	0.7HisTyr: 0.7 ± 0.029	0.0HisXaa: 0.0 ± 0.0
Ile	6.096IleAla: 6.096 ± 0.097	1.351IleCys: 1.351 ± 0.032	4.782IleAsp: 4.782 ± 0.076	4.604IleGlu: 4.604 ± 0.068	2.889IlePhe: 2.889 ± 0.069	4.996IleGly: 4.996 ± 0.086	1.096IleHis: 1.096 ± 0.038	5.559IleIle: 5.559 ± 0.098	4.765IleLys: 4.765 ± 0.076	5.574IleLeu: 5.574 ± 0.097	2.064IleMet: 2.064 ± 0.058	3.517IleAsn: 3.517 ± 0.076	3.043IlePro: 3.043 ± 0.065	1.632IleGln: 1.632 ± 0.039	3.098IleArg: 3.098 ± 0.065	5.559IleSer: 5.559 ± 0.082	4.706IleThr: 4.706 ± 0.073	4.639IleVal: 4.639 ± 0.068	0.564IleTrp: 0.564 ± 0.025	2.735IleTyr: 2.735 ± 0.053	0.0IleXaa: 0.0 ± 0.0
Lys	5.842LysAla: 5.842 ± 0.085	0.941LysCys: 0.941 ± 0.032	4.008LysAsp: 4.008 ± 0.074	4.952LysGlu: 4.952 ± 0.069	2.342LysPhe: 2.342 ± 0.055	4.398LysGly: 4.398 ± 0.069	1.109LysHis: 1.109 ± 0.032	4.506LysIle: 4.506 ± 0.069	6.157LysLys: 6.157 ± 0.1	6.511LysLeu: 6.511 ± 0.096	2.125LysMet: 2.125 ± 0.051	4.03LysAsn: 4.03 ± 0.065	2.316LysPro: 2.316 ± 0.066	1.969LysGln: 1.969 ± 0.048	2.928LysArg: 2.928 ± 0.06	4.009LysSer: 4.009 ± 0.065	4.04LysThr: 4.04 ± 0.068	3.967LysVal: 3.967 ± 0.07	0.646LysTrp: 0.646 ± 0.026	3.44LysTyr: 3.44 ± 0.071	0.0LysXaa: 0.0 ± 0.0
Leu	6.258LeuAla: 6.258 ± 0.096	1.617LeuCys: 1.617 ± 0.048	5.077LeuAsp: 5.077 ± 0.083	4.991LeuGlu: 4.991 ± 0.088	3.467LeuPhe: 3.467 ± 0.071	5.437LeuGly: 5.437 ± 0.075	1.289LeuHis: 1.289 ± 0.046	5.963LeuIle: 5.963 ± 0.101	5.447LeuLys: 5.447 ± 0.077	7.018LeuLeu: 7.018 ± 0.12	2.457LeuMet: 2.457 ± 0.052	3.572LeuAsn: 3.572 ± 0.064	3.201LeuPro: 3.201 ± 0.063	1.756LeuGln: 1.756 ± 0.045	3.809LeuArg: 3.809 ± 0.082	6.192LeuSer: 6.192 ± 0.093	5.071LeuThr: 5.071 ± 0.072	4.468LeuVal: 4.468 ± 0.074	0.672LeuTrp: 0.672 ± 0.028	3.151LeuTyr: 3.151 ± 0.068	0.001LeuXaa: 0.001 ± 0.001
Met	2.143MetAla: 2.143 ± 0.051	0.421MetCys: 0.421 ± 0.02	1.53MetAsp: 1.53 ± 0.04	1.726MetGlu: 1.726 ± 0.042	1.122MetPhe: 1.122 ± 0.041	1.815MetGly: 1.815 ± 0.042	0.446MetHis: 0.446 ± 0.023	1.97MetIle: 1.97 ± 0.053	2.444MetLys: 2.444 ± 0.05	2.579MetLeu: 2.579 ± 0.053	0.694MetMet: 0.694 ± 0.026	1.57MetAsn: 1.57 ± 0.039	1.163MetPro: 1.163 ± 0.035	0.874MetGln: 0.874 ± 0.029	1.208MetArg: 1.208 ± 0.036	1.986MetSer: 1.986 ± 0.045	1.754MetThr: 1.754 ± 0.04	1.409MetVal: 1.409 ± 0.044	0.24MetTrp: 0.24 ± 0.016	0.963MetTyr: 0.963 ± 0.032	0.0MetXaa: 0.0 ± 0.0
Asn	3.775AsnAla: 3.775 ± 0.069	0.872AsnCys: 0.872 ± 0.035	3.055AsnAsp: 3.055 ± 0.06	3.11AsnGlu: 3.11 ± 0.059	1.768AsnPhe: 1.768 ± 0.037	4.478AsnGly: 4.478 ± 0.099	0.663AsnHis: 0.663 ± 0.025	4.01AsnIle: 4.01 ± 0.056	3.15AsnLys: 3.15 ± 0.061	2.911AsnLeu: 2.911 ± 0.054	1.318AsnMet: 1.318 ± 0.041	2.656AsnAsn: 2.656 ± 0.099	1.84AsnPro: 1.84 ± 0.052	1.074AsnGln: 1.074 ± 0.075	1.744AsnArg: 1.744 ± 0.04	3.092AsnSer: 3.092 ± 0.058	2.785AsnThr: 2.785 ± 0.068	3.086AsnVal: 3.086 ± 0.063	0.465AsnTrp: 0.465 ± 0.026	2.183AsnTyr: 2.183 ± 0.052	0.0AsnXaa: 0.0 ± 0.0
Pro	2.577ProAla: 2.577 ± 0.063	0.459ProCys: 0.459 ± 0.023	2.823ProAsp: 2.823 ± 0.061	3.265ProGlu: 3.265 ± 0.072	1.459ProPhe: 1.459 ± 0.04	1.228ProGly: 1.228 ± 0.037	0.556ProHis: 0.556 ± 0.024	1.83ProIle: 1.83 ± 0.047	2.184ProLys: 2.184 ± 0.054	2.523ProLeu: 2.523 ± 0.054	0.884ProMet: 0.884 ± 0.032	1.505ProAsn: 1.505 ± 0.046	0.99ProPro: 0.99 ± 0.042	1.143ProGln: 1.143 ± 0.041	0.959ProArg: 0.959 ± 0.033	1.9ProSer: 1.9 ± 0.048	1.754ProThr: 1.754 ± 0.043	3.1ProVal: 3.1 ± 0.071	0.274ProTrp: 0.274 ± 0.02	1.384ProTyr: 1.384 ± 0.043	0.0ProXaa: 0.0 ± 0.0
Gln	1.776GlnAla: 1.776 ± 0.044	0.356GlnCys: 0.356 ± 0.02	1.122GlnAsp: 1.122 ± 0.035	1.601GlnGlu: 1.601 ± 0.037	1.078GlnPhe: 1.078 ± 0.037	1.632GlnGly: 1.632 ± 0.051	0.414GlnHis: 0.414 ± 0.024	1.782GlnIle: 1.782 ± 0.04	1.918GlnLys: 1.918 ± 0.043	2.528GlnLeu: 2.528 ± 0.052	0.755GlnMet: 0.755 ± 0.029	1.437GlnAsn: 1.437 ± 0.061	0.949GlnPro: 0.949 ± 0.038	1.16GlnGln: 1.16 ± 0.109	1.229GlnArg: 1.229 ± 0.042	1.415GlnSer: 1.415 ± 0.044	1.363GlnThr: 1.363 ± 0.041	1.343GlnVal: 1.343 ± 0.037	0.275GlnTrp: 0.275 ± 0.026	1.125GlnTyr: 1.125 ± 0.038	0.0GlnXaa: 0.0 ± 0.0
Arg	2.587ArgAla: 2.587 ± 0.059	0.631ArgCys: 0.631 ± 0.028	2.246ArgAsp: 2.246 ± 0.052	3.11ArgGlu: 3.11 ± 0.065	1.952ArgPhe: 1.952 ± 0.053	2.306ArgGly: 2.306 ± 0.049	0.73ArgHis: 0.73 ± 0.024	3.35ArgIle: 3.35 ± 0.064	3.071ArgLys: 3.071 ± 0.06	3.533ArgLeu: 3.533 ± 0.082	1.139ArgMet: 1.139 ± 0.038	2.001ArgAsn: 2.001 ± 0.043	1.156ArgPro: 1.156 ± 0.035	1.202ArgGln: 1.202 ± 0.041	2.135ArgArg: 2.135 ± 0.062	2.611ArgSer: 2.611 ± 0.052	2.208ArgThr: 2.208 ± 0.052	2.342ArgVal: 2.342 ± 0.052	0.367ArgTrp: 0.367 ± 0.018	1.832ArgTyr: 1.832 ± 0.048	0.0ArgXaa: 0.0 ± 0.0
Ser	5.608SerAla: 5.608 ± 0.089	1.045SerCys: 1.045 ± 0.035	4.652SerAsp: 4.652 ± 0.067	4.653SerGlu: 4.653 ± 0.076	3.235SerPhe: 3.235 ± 0.07	5.779SerGly: 5.779 ± 0.096	0.995SerHis: 0.995 ± 0.033	4.316SerIle: 4.316 ± 0.074	3.922SerLys: 3.922 ± 0.063	5.024SerLeu: 5.024 ± 0.075	1.586SerMet: 1.586 ± 0.041	2.635SerAsn: 2.635 ± 0.063	1.909SerPro: 1.909 ± 0.043	1.694SerGln: 1.694 ± 0.046	2.607SerArg: 2.607 ± 0.061	4.5SerSer: 4.5 ± 0.069	3.301SerThr: 3.301 ± 0.068	4.816SerVal: 4.816 ± 0.085	0.618SerTrp: 0.618 ± 0.022	2.702SerTyr: 2.702 ± 0.067	0.0SerXaa: 0.0 ± 0.0
Thr	5.876ThrAla: 5.876 ± 0.097	0.711ThrCys: 0.711 ± 0.027	3.967ThrAsp: 3.967 ± 0.07	4.3ThrGlu: 4.3 ± 0.073	2.303ThrPhe: 2.303 ± 0.044	4.586ThrGly: 4.586 ± 0.077	0.858ThrHis: 0.858 ± 0.03	3.891ThrIle: 3.891 ± 0.073	3.209ThrLys: 3.209 ± 0.069	4.396ThrLeu: 4.396 ± 0.073	1.289ThrMet: 1.289 ± 0.039	2.272ThrAsn: 2.272 ± 0.063	2.15ThrPro: 2.15 ± 0.059	1.299ThrGln: 1.299 ± 0.041	1.691ThrArg: 1.691 ± 0.039	3.636ThrSer: 3.636 ± 0.076	5.484ThrThr: 5.484 ± 0.317	4.937ThrVal: 4.937 ± 0.104	0.407ThrTrp: 0.407 ± 0.022	2.173ThrTyr: 2.173 ± 0.067	0.0ThrXaa: 0.0 ± 0.0
Val	3.986ValAla: 3.986 ± 0.069	1.305ValCys: 1.305 ± 0.04	3.873ValAsp: 3.873 ± 0.067	3.908ValGlu: 3.908 ± 0.064	3.161ValPhe: 3.161 ± 0.063	3.81ValGly: 3.81 ± 0.067	1.07ValHis: 1.07 ± 0.033	5.267ValIle: 5.267 ± 0.088	4.569ValLys: 4.569 ± 0.079	5.682ValLeu: 5.682 ± 0.103	1.89ValMet: 1.89 ± 0.048	3.114ValAsn: 3.114 ± 0.06	2.538ValPro: 2.538 ± 0.053	1.587ValGln: 1.587 ± 0.038	2.682ValArg: 2.682 ± 0.053	4.919ValSer: 4.919 ± 0.078	4.564ValThr: 4.564 ± 0.086	4.046ValVal: 4.046 ± 0.079	0.577ValTrp: 0.577 ± 0.021	2.646ValTyr: 2.646 ± 0.054	0.0ValXaa: 0.0 ± 0.0
Trp	0.558TrpAla: 0.558 ± 0.025	0.173TrpCys: 0.173 ± 0.014	0.53TrpAsp: 0.53 ± 0.027	0.552TrpGlu: 0.552 ± 0.024	0.464TrpPhe: 0.464 ± 0.021	0.689TrpGly: 0.689 ± 0.031	0.211TrpHis: 0.211 ± 0.014	0.556TrpIle: 0.556 ± 0.023	0.584TrpLys: 0.584 ± 0.028	0.852TrpLeu: 0.852 ± 0.031	0.203TrpMet: 0.203 ± 0.014	0.596TrpAsn: 0.596 ± 0.049	0.132TrpPro: 0.132 ± 0.013	0.326TrpGln: 0.326 ± 0.021	0.367TrpArg: 0.367 ± 0.02	0.545TrpSer: 0.545 ± 0.026	0.478TrpThr: 0.478 ± 0.024	0.439TrpVal: 0.439 ± 0.021	0.114TrpTrp: 0.114 ± 0.009	0.433TrpTyr: 0.433 ± 0.024	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.984TyrAla: 2.984 ± 0.052	0.783TyrCys: 0.783 ± 0.028	3.203TyrAsp: 3.203 ± 0.072	2.713TyrGlu: 2.713 ± 0.059	1.87TyrPhe: 1.87 ± 0.047	3.124TyrGly: 3.124 ± 0.066	0.732TyrHis: 0.732 ± 0.027	3.18TyrIle: 3.18 ± 0.064	2.634TyrLys: 2.634 ± 0.05	3.141TyrLeu: 3.141 ± 0.063	1.099TyrMet: 1.099 ± 0.037	2.19TyrAsn: 2.19 ± 0.06	1.409TyrPro: 1.409 ± 0.045	0.933TyrGln: 0.933 ± 0.028	1.842TyrArg: 1.842 ± 0.044	3.059TyrSer: 3.059 ± 0.065	2.616TyrThr: 2.616 ± 0.074	2.507TyrVal: 2.507 ± 0.049	0.365TyrTrp: 0.365 ± 0.021	2.055TyrTyr: 2.055 ± 0.058	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.001XaaVal: 0.001 ± 0.001	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.003XaaXaa: 0.003 ± 0.003

Statistics based on 2780 proteins (979500 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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