Zavarzinia aquatilis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodospirillales; Zavarziniaceae; Zavarzinia

Average proteome isoelectric point is 6.46

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4178 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A317E009|A0A317E009_9PROT Uncharacterized protein OS=Zavarzinia aquatilis OX=2211142 GN=DKG74_15500 PE=3 SV=1
MM1 pKa = 7.46NFTGTNNDD9 pKa = 3.04DD10 pKa = 4.04TYY12 pKa = 11.3NGTSAADD19 pKa = 3.74VFDD22 pKa = 4.0MAQGGVDD29 pKa = 2.92IVNGKK34 pKa = 9.82EE35 pKa = 3.84GDD37 pKa = 3.5DD38 pKa = 3.53EE39 pKa = 4.76FRR41 pKa = 11.84FGATLTAADD50 pKa = 4.45RR51 pKa = 11.84VIGGDD56 pKa = 3.6GYY58 pKa = 11.43DD59 pKa = 3.55HH60 pKa = 7.27VILKK64 pKa = 10.45GDD66 pKa = 3.58YY67 pKa = 10.29SAGLVLSSTTLRR79 pKa = 11.84YY80 pKa = 9.84VEE82 pKa = 4.36SFEE85 pKa = 4.13MTSGFSYY92 pKa = 10.69DD93 pKa = 2.82ITMSNGNLQAGEE105 pKa = 4.22VMTVSAYY112 pKa = 10.2GVGFGGSGHH121 pKa = 5.84YY122 pKa = 9.67FHH124 pKa = 7.55FNGSAEE130 pKa = 3.93TDD132 pKa = 3.06GRR134 pKa = 11.84FNVSDD139 pKa = 4.49GYY141 pKa = 11.64GDD143 pKa = 5.43DD144 pKa = 4.26ILIGSQGGDD153 pKa = 3.93SLSMSYY159 pKa = 10.81GGNDD163 pKa = 3.67YY164 pKa = 10.98IDD166 pKa = 4.01GQGGGDD172 pKa = 3.67VIMAEE177 pKa = 4.29NNFGAGDD184 pKa = 4.65FINGGAGSDD193 pKa = 2.71IVYY196 pKa = 10.1FRR198 pKa = 11.84GLQSATIILNGANFQNIEE216 pKa = 4.01TLAVVGNYY224 pKa = 9.87GADD227 pKa = 3.48FQVADD232 pKa = 4.01TLLAAGQWLNLDD244 pKa = 3.62MRR246 pKa = 11.84SVQAGQTVNFIGSLEE261 pKa = 3.95TDD263 pKa = 3.09GRR265 pKa = 11.84FDD267 pKa = 4.03YY268 pKa = 11.52YY269 pKa = 11.21DD270 pKa = 3.69GAGNDD275 pKa = 3.58HH276 pKa = 6.18FTGGGGADD284 pKa = 4.45LYY286 pKa = 11.03RR287 pKa = 11.84GGNGGSDD294 pKa = 2.89IVSGLGGDD302 pKa = 3.7DD303 pKa = 3.69YY304 pKa = 11.36IYY306 pKa = 10.86FGGDD310 pKa = 3.3LDD312 pKa = 4.93SGDD315 pKa = 4.53TIWGGAGYY323 pKa = 10.68DD324 pKa = 3.9VMDD327 pKa = 3.92LTGDD331 pKa = 4.12LSAGVHH337 pKa = 5.71FADD340 pKa = 5.63DD341 pKa = 3.56GLAGVEE347 pKa = 3.82RR348 pKa = 11.84VVLRR352 pKa = 11.84DD353 pKa = 3.08GFTYY357 pKa = 11.04KK358 pKa = 10.84LFFADD363 pKa = 3.98GNLNTGEE370 pKa = 4.25GLLISSSGNIGAGNSVYY387 pKa = 10.27IDD389 pKa = 3.67ASAEE393 pKa = 3.55TDD395 pKa = 3.2AAYY398 pKa = 9.75TMYY401 pKa = 11.02DD402 pKa = 2.95SDD404 pKa = 5.8GNDD407 pKa = 3.27TLIGGGGNDD416 pKa = 3.95VFWSLAGGRR425 pKa = 11.84DD426 pKa = 3.49KK427 pKa = 11.57LIGNGGNDD435 pKa = 3.53TFVVAGPLHH444 pKa = 7.09AYY446 pKa = 10.2DD447 pKa = 4.68SYY449 pKa = 12.09NGGADD454 pKa = 3.06IDD456 pKa = 4.35TITIYY461 pKa = 10.9NADD464 pKa = 3.69KK465 pKa = 11.24GGVIDD470 pKa = 5.79LSTGKK475 pKa = 10.23ISIGGVAGGSVTQIEE490 pKa = 4.9NISGSAYY497 pKa = 10.55ADD499 pKa = 3.41TLTGNSAANFIDD511 pKa = 4.18GGAGNDD517 pKa = 4.26TISGGQGSDD526 pKa = 3.67DD527 pKa = 3.67LRR529 pKa = 11.84GGAGSDD535 pKa = 3.73KK536 pKa = 10.95LDD538 pKa = 3.98GGAKK542 pKa = 10.13DD543 pKa = 4.64DD544 pKa = 4.53ILYY547 pKa = 10.92GDD549 pKa = 4.71AGNDD553 pKa = 3.1ILLGGQGNDD562 pKa = 3.19NLFGGDD568 pKa = 3.17NTDD571 pKa = 3.99RR572 pKa = 11.84LDD574 pKa = 3.87GGIGNDD580 pKa = 4.36DD581 pKa = 3.59LTGGAGRR588 pKa = 11.84DD589 pKa = 3.29TFVFSTGTGVDD600 pKa = 3.28HH601 pKa = 7.61VKK603 pKa = 10.99DD604 pKa = 3.66FTNNFDD610 pKa = 3.92YY611 pKa = 11.51LDD613 pKa = 3.97FTGVAADD620 pKa = 3.74FADD623 pKa = 3.82IQAHH627 pKa = 5.31MSQVGTDD634 pKa = 3.21VVIAYY639 pKa = 7.49GTDD642 pKa = 3.16KK643 pKa = 11.14FILEE647 pKa = 4.14NTSIGVLDD655 pKa = 4.43ASDD658 pKa = 3.81FLVV661 pKa = 3.42

Molecular weight: 67.75 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A317EBL6|A0A317EBL6_9PROT Cysteine synthase OS=Zavarzinia aquatilis OX=2211142 GN=cysK PE=4 SV=1
MM1 pKa = 7.47RR2 pKa = 11.84RR3 pKa = 11.84RR4 pKa = 11.84RR5 pKa = 11.84RR6 pKa = 11.84RR7 pKa = 11.84PSRR10 pKa = 11.84RR11 pKa = 11.84PRR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84RR16 pKa = 11.84SRR18 pKa = 11.84HH19 pKa = 5.1RR20 pKa = 11.84APARR24 pKa = 11.84PPPRR28 pKa = 11.84RR29 pKa = 11.84KK30 pKa = 9.32PRR32 pKa = 11.84RR33 pKa = 11.84RR34 pKa = 11.84PMKK37 pKa = 9.99RR38 pKa = 11.84RR39 pKa = 11.84PASTLPASPPMAPPCWPAARR59 pKa = 11.84RR60 pKa = 11.84ATVRR64 pKa = 3.38

Molecular weight: 7.79 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4178

0

4178

1369053

26

4300

327.7

35.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.587 ± 0.062

0.813 ± 0.011

6.009 ± 0.036

5.376 ± 0.036

3.617 ± 0.022

9.522 ± 0.057

1.931 ± 0.021

4.948 ± 0.025

2.891 ± 0.028

10.412 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.269 ± 0.017

2.288 ± 0.022

5.357 ± 0.039

2.684 ± 0.021

7.329 ± 0.045

4.823 ± 0.028

5.197 ± 0.036

7.532 ± 0.037

1.281 ± 0.017

2.136 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski