Streptococcus phage Javan484

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 71 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6B715|A0A4D6B715_9CAUD VRR-NUC domain-containing protein OS=Streptococcus phage Javan484 OX=2548199 GN=Javan484_0046 PE=4 SV=1
MM1 pKa = 7.82LIYY4 pKa = 10.15PATFTQDD11 pKa = 2.07TDD13 pKa = 4.11YY14 pKa = 11.77IMVTFPDD21 pKa = 3.7VPEE24 pKa = 5.63AITQGEE30 pKa = 4.54DD31 pKa = 3.17FQEE34 pKa = 4.6AYY36 pKa = 10.36EE37 pKa = 4.17MAVEE41 pKa = 4.15VLGFALEE48 pKa = 5.25DD49 pKa = 3.43YY50 pKa = 10.49TDD52 pKa = 3.81YY53 pKa = 11.16PKK55 pKa = 11.16ASAVSDD61 pKa = 3.95LKK63 pKa = 10.93EE64 pKa = 3.92QYY66 pKa = 9.46PDD68 pKa = 3.46SDD70 pKa = 3.42IALIGIDD77 pKa = 3.16MVAYY81 pKa = 7.98MKK83 pKa = 10.29KK84 pKa = 8.58YY85 pKa = 9.49HH86 pKa = 5.88SKK88 pKa = 10.45KK89 pKa = 8.6VRR91 pKa = 11.84KK92 pKa = 9.32NVTIPEE98 pKa = 4.28WLNDD102 pKa = 3.5AAEE105 pKa = 4.41DD106 pKa = 3.75KK107 pKa = 10.95NLNFSQVLTEE117 pKa = 4.09ALEE120 pKa = 4.63LKK122 pKa = 10.44LQAA125 pKa = 4.95

Molecular weight: 14.23 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6B642|A0A4D6B642_9CAUD Holin OS=Streptococcus phage Javan484 OX=2548199 GN=Javan484_0007 PE=4 SV=1
MM1 pKa = 7.83ADD3 pKa = 2.89ISLKK7 pKa = 10.51VVGTAGLKK15 pKa = 10.29KK16 pKa = 10.32KK17 pKa = 10.89LEE19 pKa = 4.57LIIKK23 pKa = 9.55KK24 pKa = 10.33DD25 pKa = 3.41AVKK28 pKa = 10.59KK29 pKa = 9.97IVRR32 pKa = 11.84DD33 pKa = 3.31NGTQLQRR40 pKa = 11.84KK41 pKa = 7.23MINKK45 pKa = 9.27AVFTKK50 pKa = 10.48GYY52 pKa = 8.22STGATRR58 pKa = 11.84RR59 pKa = 11.84SITMQIGDD67 pKa = 3.93GGLSVKK73 pKa = 9.97VKK75 pKa = 10.48PGTHH79 pKa = 4.95YY80 pKa = 10.9AGYY83 pKa = 10.11LEE85 pKa = 4.73RR86 pKa = 11.84GTRR89 pKa = 11.84LMSKK93 pKa = 10.05QPFVLPALKK102 pKa = 9.62EE103 pKa = 4.0QKK105 pKa = 10.45VKK107 pKa = 10.34FRR109 pKa = 11.84KK110 pKa = 10.07DD111 pKa = 2.98LEE113 pKa = 4.36ALVKK117 pKa = 10.71

Molecular weight: 12.97 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

71

0

71

13929

37

1207

196.2

22.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.734 ± 0.802

0.51 ± 0.077

6.591 ± 0.35

7.23 ± 0.504

4.379 ± 0.206

6.196 ± 0.466

1.558 ± 0.14

7.395 ± 0.172

9.017 ± 0.461

8.752 ± 0.32

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.541 ± 0.163

5.277 ± 0.278

2.419 ± 0.153

4.013 ± 0.216

4.02 ± 0.241

6.289 ± 0.427

6.339 ± 0.211

5.995 ± 0.25

0.984 ± 0.112

3.762 ± 0.314
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski