Psychroflexus salarius

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Psychroflexus

Average proteome isoelectric point is 6.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2311 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1M4XUK2|A0A1M4XUK2_9FLAO Glycosyltransferase catalytic subunit of cellulose synthase and poly-beta-1 6-N-acetylglucosamine synthase OS=Psychroflexus salarius OX=1155689 GN=SAMN05444278_11062 PE=4 SV=1
MM1 pKa = 7.69NYY3 pKa = 10.57NYY5 pKa = 10.71LLSCLLSLTLFSLGFSQSNPDD26 pKa = 3.56FYY28 pKa = 11.3LAEE31 pKa = 4.3NGVTCMCPDD40 pKa = 4.09ADD42 pKa = 4.8FGDD45 pKa = 4.32TGTLTINGVEE55 pKa = 3.97KK56 pKa = 10.26TFTKK60 pKa = 10.46RR61 pKa = 11.84SRR63 pKa = 11.84NLLYY67 pKa = 10.53AYY69 pKa = 9.7IDD71 pKa = 3.96VNPQDD76 pKa = 3.88PEE78 pKa = 3.84IALTCTSGITDD89 pKa = 4.52LSNAFNSKK97 pKa = 11.03SNFNQDD103 pKa = 2.82LSHH106 pKa = 6.89WDD108 pKa = 3.4VSNVTNMYY116 pKa = 10.94RR117 pKa = 11.84MFYY120 pKa = 9.33YY121 pKa = 10.87ANAFNQPLNNWDD133 pKa = 3.45VSNVTNMSDD142 pKa = 3.52MIYY145 pKa = 10.43SADD148 pKa = 4.17VFNQPLNNWDD158 pKa = 3.45VSNVTNMSNMFPGAIAFNQPLNNWDD183 pKa = 3.45VSNVTNMRR191 pKa = 11.84GMFYY195 pKa = 10.64GADD198 pKa = 3.38AFNQPLNNWDD208 pKa = 3.44VSSVTNLSVMFVDD221 pKa = 5.65ANAFNQPLDD230 pKa = 3.47NWDD233 pKa = 3.49VSSVTEE239 pKa = 3.97MNAMFTNATNFSQDD253 pKa = 3.02LSNWCVEE260 pKa = 4.84LITDD264 pKa = 5.02FPTSFASGSSMIPEE278 pKa = 4.76YY279 pKa = 10.97YY280 pKa = 9.5PDD282 pKa = 3.41WGAEE286 pKa = 4.02CSNAGTTTPVITLLGEE302 pKa = 4.04NPVTIEE308 pKa = 3.96VGTTYY313 pKa = 10.9TDD315 pKa = 3.35AGATASDD322 pKa = 3.88NTDD325 pKa = 2.8GDD327 pKa = 3.96LTDD330 pKa = 5.77DD331 pKa = 4.38IIVTGSVDD339 pKa = 3.21TDD341 pKa = 3.64VVGTYY346 pKa = 8.31TLSYY350 pKa = 11.06DD351 pKa = 3.73VTDD354 pKa = 3.66SSGNAAEE361 pKa = 4.41TVTRR365 pKa = 11.84TVNVVDD371 pKa = 3.41TTIPVITLLGDD382 pKa = 3.47NPVTIEE388 pKa = 4.02VGTTYY393 pKa = 10.9TDD395 pKa = 3.35AGATASDD402 pKa = 3.88NTDD405 pKa = 2.89GDD407 pKa = 3.83LTADD411 pKa = 4.13IIITGNVDD419 pKa = 3.08ADD421 pKa = 3.89VVGTYY426 pKa = 8.35TLSYY430 pKa = 11.06DD431 pKa = 3.73VTDD434 pKa = 3.66SSGNAAEE441 pKa = 4.24TVTRR445 pKa = 11.84IVNVVDD451 pKa = 3.15TTIPVISLLGDD462 pKa = 3.33NPVTIEE468 pKa = 3.99VSTTYY473 pKa = 10.66TDD475 pKa = 3.55AGATASDD482 pKa = 4.06NADD485 pKa = 3.12GDD487 pKa = 3.85LTANIIVTGSVDD499 pKa = 3.21TDD501 pKa = 3.64VVGTYY506 pKa = 8.31TLSYY510 pKa = 11.06DD511 pKa = 3.73VTDD514 pKa = 3.66SSGNAAEE521 pKa = 4.41TVTRR525 pKa = 11.84TVNVVGTTNPDD536 pKa = 3.23FYY538 pKa = 10.68IAEE541 pKa = 4.26NGVTCMCPDD550 pKa = 4.09ADD552 pKa = 4.8FGDD555 pKa = 4.32TGTLTINGEE564 pKa = 4.14EE565 pKa = 3.94KK566 pKa = 10.24TFTKK570 pKa = 9.83RR571 pKa = 11.84TRR573 pKa = 11.84AQLDD577 pKa = 3.73AIIANNQQDD586 pKa = 3.9PEE588 pKa = 4.15IALTCTSGITSMASLFSGKK607 pKa = 8.58DD608 pKa = 3.04TFNQDD613 pKa = 2.19ISHH616 pKa = 6.91WDD618 pKa = 3.32VSNVNNMQSAFIGASSFNQDD638 pKa = 1.91ISLWNTSNLVTMADD652 pKa = 3.58MFRR655 pKa = 11.84GASSFNNPLNNWDD668 pKa = 3.38VSNVVNMFSTFASASSFNQPLNNWDD693 pKa = 3.59VSSVTVMKK701 pKa = 10.88QMFASAEE708 pKa = 4.05AFNQSLDD715 pKa = 3.25NWDD718 pKa = 3.63VSNVTDD724 pKa = 3.61MSRR727 pKa = 11.84MFRR730 pKa = 11.84SAGSFNQTLNNWDD743 pKa = 3.53VSSVTNMNQMFAFADD758 pKa = 3.65AFNQSLNNWDD768 pKa = 3.62VSNVTDD774 pKa = 4.24MYY776 pKa = 11.61QMFNQAVTFNKK787 pKa = 9.82QLNDD791 pKa = 3.36WNTEE795 pKa = 3.42NVTNMEE801 pKa = 3.9GMFIIASSFNQPLDD815 pKa = 3.24QWDD818 pKa = 3.73VSAVNNMEE826 pKa = 4.48GMFKK830 pKa = 10.28QATSFNQPLNNWDD843 pKa = 3.49VSSVTNMRR851 pKa = 11.84NMFSGADD858 pKa = 3.44AFNQSLNNWNVSNVTDD874 pKa = 3.53MSFMFFGAVVFNQNISNWCVEE895 pKa = 4.42QIPSEE900 pKa = 3.99PSSFSTNSPLEE911 pKa = 3.98EE912 pKa = 4.0SFEE915 pKa = 4.8PNWGAEE921 pKa = 4.23CEE923 pKa = 4.36SLSIDD928 pKa = 3.98NPTEE932 pKa = 3.68AQLAIKK938 pKa = 9.83LYY940 pKa = 9.49PNPATHH946 pKa = 6.44QVTLTSNANADD957 pKa = 3.6VKK959 pKa = 10.96AVSLYY964 pKa = 11.4NMLGQKK970 pKa = 9.43VKK972 pKa = 10.54QLEE975 pKa = 4.34LSGSVAPIQIDD986 pKa = 3.83VSDD989 pKa = 4.57LAAGNYY995 pKa = 8.88FIQITTKK1002 pKa = 10.24NNTRR1006 pKa = 11.84ITKK1009 pKa = 10.21RR1010 pKa = 11.84FIKK1013 pKa = 10.39RR1014 pKa = 3.11

Molecular weight: 110.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1M4W4B9|A0A1M4W4B9_9FLAO Nucleotide-binding universal stress protein UspA family OS=Psychroflexus salarius OX=1155689 GN=SAMN05444278_10575 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.59RR3 pKa = 11.84TFQPSKK9 pKa = 10.15RR10 pKa = 11.84KK11 pKa = 9.6RR12 pKa = 11.84KK13 pKa = 8.29NKK15 pKa = 9.34HH16 pKa = 4.03GFRR19 pKa = 11.84EE20 pKa = 4.09RR21 pKa = 11.84MASKK25 pKa = 10.6NGRR28 pKa = 11.84KK29 pKa = 9.11VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84SKK37 pKa = 10.67GRR39 pKa = 11.84KK40 pKa = 7.79KK41 pKa = 10.7LSVSSEE47 pKa = 2.78ISMRR51 pKa = 11.84KK52 pKa = 8.72RR53 pKa = 3.06

Molecular weight: 6.4 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2311

0

2311

777795

32

2412

336.6

38.06

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.334 ± 0.05

0.708 ± 0.016

5.564 ± 0.045

6.496 ± 0.044

5.379 ± 0.04

5.844 ± 0.056

1.869 ± 0.024

7.858 ± 0.047

7.772 ± 0.081

9.675 ± 0.062

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.948 ± 0.027

6.472 ± 0.06

3.264 ± 0.025

4.235 ± 0.038

3.281 ± 0.034

6.815 ± 0.043

5.598 ± 0.055

5.99 ± 0.044

0.949 ± 0.019

3.947 ± 0.039
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski