Winogradskyella sp. J14-2

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Winogradskyella; unclassified Winogradskyella

Average proteome isoelectric point is 6.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2928 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1P8RU77|A0A1P8RU77_9FLAO Chaperone protein ClpB OS=Winogradskyella sp. J14-2 OX=1936080 GN=clpB PE=3 SV=1
MM1 pKa = 7.57KK2 pKa = 10.14SIKK5 pKa = 10.3LILSLAVVLTLTISCEE21 pKa = 4.15DD22 pKa = 4.31DD23 pKa = 4.03DD24 pKa = 4.84VAVPDD29 pKa = 3.59VEE31 pKa = 4.2VRR33 pKa = 11.84DD34 pKa = 3.88RR35 pKa = 11.84GEE37 pKa = 3.61QQIADD42 pKa = 3.82NDD44 pKa = 4.07SLLGYY49 pKa = 10.61LSTHH53 pKa = 6.6YY54 pKa = 10.73YY55 pKa = 10.66NSDD58 pKa = 3.36FFLTGTNHH66 pKa = 7.35KK67 pKa = 8.99YY68 pKa = 8.92TDD70 pKa = 3.31IVVTEE75 pKa = 4.07LVEE78 pKa = 4.53GEE80 pKa = 4.52VVPAGHH86 pKa = 6.72TLLINAVEE94 pKa = 4.22THH96 pKa = 4.94TTTFEE101 pKa = 4.01EE102 pKa = 3.8TDD104 pKa = 3.34YY105 pKa = 11.32QYY107 pKa = 11.92YY108 pKa = 9.83VLKK111 pKa = 10.51LNQGAGDD118 pKa = 3.86SPRR121 pKa = 11.84FTDD124 pKa = 4.4FVRR127 pKa = 11.84MRR129 pKa = 11.84YY130 pKa = 9.59EE131 pKa = 4.17GFTLEE136 pKa = 4.61DD137 pKa = 3.72GEE139 pKa = 4.83VFDD142 pKa = 6.13SRR144 pKa = 11.84ATPEE148 pKa = 4.42DD149 pKa = 4.07LPMQGVGFSGGVIRR163 pKa = 11.84GWQLTIPMFNTAEE176 pKa = 4.09SFAIGSDD183 pKa = 4.21GITNYY188 pKa = 10.83NNFGLGMMFLPSGLAYY204 pKa = 10.52FSNTASGSVAAYY216 pKa = 10.2SNLVFKK222 pKa = 11.02FEE224 pKa = 4.16LLQYY228 pKa = 10.75EE229 pKa = 4.79EE230 pKa = 4.99IDD232 pKa = 3.54HH233 pKa = 7.27DD234 pKa = 4.62GDD236 pKa = 4.79GIPSYY241 pKa = 11.02IEE243 pKa = 5.22DD244 pKa = 4.17LDD246 pKa = 4.82ANLDD250 pKa = 3.86VLDD253 pKa = 5.78DD254 pKa = 4.42DD255 pKa = 4.55TDD257 pKa = 3.56EE258 pKa = 5.45DD259 pKa = 4.26LAPNYY264 pKa = 10.52VDD266 pKa = 5.36VDD268 pKa = 4.06DD269 pKa = 6.48DD270 pKa = 4.36NDD272 pKa = 3.98GVNTRR277 pKa = 11.84DD278 pKa = 3.23EE279 pKa = 4.59LMPTSYY285 pKa = 10.24TVDD288 pKa = 2.82TNMNEE293 pKa = 4.13EE294 pKa = 4.29EE295 pKa = 4.27PVLGPGEE302 pKa = 4.14YY303 pKa = 9.89EE304 pKa = 3.84ISRR307 pKa = 11.84STSNGILTINTVKK320 pKa = 10.65VVDD323 pKa = 4.05SDD325 pKa = 4.76SNGTPDD331 pKa = 4.4YY332 pKa = 10.08LQEE335 pKa = 5.77DD336 pKa = 3.28IAEE339 pKa = 4.43NYY341 pKa = 10.04NEE343 pKa = 4.17EE344 pKa = 4.24EE345 pKa = 4.4GG346 pKa = 3.89

Molecular weight: 38.28 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1P8RZT9|A0A1P8RZT9_9FLAO GTP cyclohydrolase 1 OS=Winogradskyella sp. J14-2 OX=1936080 GN=folE PE=3 SV=1
MM1 pKa = 8.0PKK3 pKa = 9.06RR4 pKa = 11.84TFQPSKK10 pKa = 9.13RR11 pKa = 11.84KK12 pKa = 9.48RR13 pKa = 11.84RR14 pKa = 11.84NKK16 pKa = 9.49HH17 pKa = 3.94GFRR20 pKa = 11.84EE21 pKa = 4.18RR22 pKa = 11.84MASVNGRR29 pKa = 11.84KK30 pKa = 9.18VLASRR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.05GRR40 pKa = 11.84KK41 pKa = 8.34KK42 pKa = 10.66LSVSSEE48 pKa = 3.9LRR50 pKa = 11.84HH51 pKa = 6.16KK52 pKa = 10.53KK53 pKa = 10.04

Molecular weight: 6.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2928

0

2928

1008313

38

5318

344.4

38.84

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.311 ± 0.048

0.769 ± 0.02

6.002 ± 0.05

6.534 ± 0.044

5.218 ± 0.038

6.317 ± 0.057

1.706 ± 0.028

7.934 ± 0.048

7.594 ± 0.09

9.118 ± 0.061

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.038 ± 0.029

6.622 ± 0.055

3.299 ± 0.024

3.26 ± 0.026

3.339 ± 0.037

6.425 ± 0.043

6.108 ± 0.089

6.238 ± 0.035

1.016 ± 0.017

4.151 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski