Ziziphus jujuba witches -broom phytoplasma

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Tenericutes; Mollicutes; Acholeplasmatales; Acholeplasmataceae; Candidatus Phytoplasma; 16SrV (Elm yellows group)

Average proteome isoelectric point is 7.93

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 597 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A660HMC7|A0A660HMC7_ZIZJU Uncharacterized protein OS=Ziziphus jujuba witches'-broom phytoplasma OX=135727 GN=CWO85_01420 PE=4 SV=1
MM1 pKa = 7.73AKK3 pKa = 10.07NNKK6 pKa = 9.61KK7 pKa = 9.53IDD9 pKa = 3.74SSSPMIDD16 pKa = 2.53ISYY19 pKa = 10.75QILSQNKK26 pKa = 7.4EE27 pKa = 3.91PMHH30 pKa = 5.7IQQIIEE36 pKa = 4.06EE37 pKa = 4.24VFKK40 pKa = 10.87IKK42 pKa = 10.72KK43 pKa = 9.98LDD45 pKa = 3.55INNKK49 pKa = 9.3DD50 pKa = 3.04ICVQLYY56 pKa = 10.73LDD58 pKa = 4.45IVSSGCFVFCGDD70 pKa = 4.66DD71 pKa = 3.24LWNIKK76 pKa = 10.12ANNLHH81 pKa = 6.61FWDD84 pKa = 4.08KK85 pKa = 11.45DD86 pKa = 3.55VFVNPNEE93 pKa = 4.18QKK95 pKa = 10.37IEE97 pKa = 3.91IPKK100 pKa = 10.05NLEE103 pKa = 3.89KK104 pKa = 10.72EE105 pKa = 3.86ILDD108 pKa = 3.83FNEE111 pKa = 4.22FDD113 pKa = 5.14LKK115 pKa = 10.9PKK117 pKa = 10.74SNEE120 pKa = 3.64EE121 pKa = 3.81NNEE124 pKa = 3.9NEE126 pKa = 4.16EE127 pKa = 4.23EE128 pKa = 4.18KK129 pKa = 11.36LNDD132 pKa = 3.49IPLDD136 pKa = 3.66SLDD139 pKa = 3.78INDD142 pKa = 3.84EE143 pKa = 4.16KK144 pKa = 11.54EE145 pKa = 3.92EE146 pKa = 4.06VEE148 pKa = 4.6IDD150 pKa = 4.85DD151 pKa = 5.14IEE153 pKa = 4.34KK154 pKa = 10.89NKK156 pKa = 10.54NNEE159 pKa = 3.99EE160 pKa = 3.62EE161 pKa = 4.29DD162 pKa = 4.0EE163 pKa = 4.52YY164 pKa = 11.14INDD167 pKa = 3.57YY168 pKa = 11.36EE169 pKa = 4.33EE170 pKa = 4.43YY171 pKa = 10.08FYY173 pKa = 11.41KK174 pKa = 10.85

Molecular weight: 20.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A660HMR2|A0A660HMR2_ZIZJU Uncharacterized protein OS=Ziziphus jujuba witches'-broom phytoplasma OX=135727 GN=CWO85_01195 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.17QPSKK9 pKa = 8.82IKK11 pKa = 10.5RR12 pKa = 11.84IRR14 pKa = 11.84THH16 pKa = 5.66GFRR19 pKa = 11.84TRR21 pKa = 11.84MSTPEE26 pKa = 3.62GRR28 pKa = 11.84RR29 pKa = 11.84VLSNRR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.8GRR39 pKa = 11.84IRR41 pKa = 11.84LTVV44 pKa = 2.91

Molecular weight: 5.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

597

0

597

180025

38

1954

301.5

35.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.41 ± 0.105

0.912 ± 0.031

4.947 ± 0.059

7.13 ± 0.107

5.924 ± 0.125

3.997 ± 0.1

1.575 ± 0.043

10.744 ± 0.141

12.027 ± 0.125

10.182 ± 0.132

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.697 ± 0.043

8.056 ± 0.119

2.609 ± 0.041

4.463 ± 0.111

2.653 ± 0.066

5.855 ± 0.079

4.941 ± 0.148

4.316 ± 0.098

0.531 ± 0.028

4.034 ± 0.078
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski