Corynebacterium sp. 2039
Average proteome isoelectric point is 5.88
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2838 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6B8VY52|A0A6B8VY52_9CORY Yqey-like protein OS=Corynebacterium sp. 2039 OX=2675219 GN=COCCU_01520 PE=4 SV=1
MM1 pKa = 7.73 IPIRR5 pKa = 11.84 KK6 pKa = 7.45 FTLTAFAILSLGLAGCSASTEE27 pKa = 4.2 DD28 pKa = 5.67 DD29 pKa = 3.66 SATTADD35 pKa = 3.69 TAASLSTPEE44 pKa = 4.73 SEE46 pKa = 4.48 TSSEE50 pKa = 4.04 STTVVTTTEE59 pKa = 4.01 EE60 pKa = 4.39 DD61 pKa = 3.58 PTPEE65 pKa = 3.94 TTVEE69 pKa = 4.28 EE70 pKa = 4.37 EE71 pKa = 4.26 SSPNPSIDD79 pKa = 3.13 ATADD83 pKa = 3.33 AGPTLVDD90 pKa = 3.43 PARR93 pKa = 11.84 FSMDD97 pKa = 2.66 GPYY100 pKa = 10.69 VFDD103 pKa = 4.04 YY104 pKa = 8.85 ATQSGEE110 pKa = 4.08 TGTCLLTPDD119 pKa = 4.36 GATCTGVPAADD130 pKa = 3.91 VPDD133 pKa = 3.33 ITYY136 pKa = 10.86 APFAGQRR143 pKa = 11.84 PGAVSASMGGIHH155 pKa = 5.45 YY156 pKa = 7.5 TILEE160 pKa = 4.39 GVPPAPAQLQPGEE173 pKa = 4.37 KK174 pKa = 9.81 LVVDD178 pKa = 4.24 ATTCEE183 pKa = 4.14 VSQTRR188 pKa = 11.84 DD189 pKa = 3.43 VTCVSGDD196 pKa = 3.27 TSFTVSGADD205 pKa = 3.57 RR206 pKa = 11.84 SITTGG211 pKa = 3.01
Molecular weight: 21.46 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.722
IPC2_protein 3.668
IPC_protein 3.656
Toseland 3.452
ProMoST 3.821
Dawson 3.643
Bjellqvist 3.808
Wikipedia 3.579
Rodwell 3.49
Grimsley 3.363
Solomon 3.63
Lehninger 3.592
Nozaki 3.77
DTASelect 3.973
Thurlkill 3.503
EMBOSS 3.592
Sillero 3.77
Patrickios 0.947
IPC_peptide 3.63
IPC2_peptide 3.757
IPC2.peptide.svr19 3.732
Protein with the highest isoelectric point:
>tr|A0A6B8W6F0|A0A6B8W6F0_9CORY Uncharacterized protein OS=Corynebacterium sp. 2039 OX=2675219 GN=COCCU_02610 PE=4 SV=1
MM1 pKa = 7.4 GSVIKK6 pKa = 10.38 KK7 pKa = 8.42 RR8 pKa = 11.84 RR9 pKa = 11.84 KK10 pKa = 9.06 RR11 pKa = 11.84 MSKK14 pKa = 9.63 KK15 pKa = 9.39 KK16 pKa = 9.04 HH17 pKa = 5.39 RR18 pKa = 11.84 KK19 pKa = 5.37 MLRR22 pKa = 11.84 RR23 pKa = 11.84 TRR25 pKa = 11.84 VQRR28 pKa = 11.84 RR29 pKa = 11.84 KK30 pKa = 10.11 LGKK33 pKa = 9.87
Molecular weight: 4.16 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.507
IPC2_protein 11.184
IPC_protein 12.749
Toseland 12.925
ProMoST 13.408
Dawson 12.925
Bjellqvist 12.91
Wikipedia 13.393
Rodwell 12.735
Grimsley 12.954
Solomon 13.408
Lehninger 13.305
Nozaki 12.91
DTASelect 12.91
Thurlkill 12.91
EMBOSS 13.408
Sillero 12.91
Patrickios 12.457
IPC_peptide 13.408
IPC2_peptide 12.398
IPC2.peptide.svr19 9.111
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2838
0
2838
925458
29
3028
326.1
35.38
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.263 ± 0.054
0.637 ± 0.011
5.53 ± 0.038
6.855 ± 0.054
3.301 ± 0.031
8.572 ± 0.043
2.219 ± 0.022
5.13 ± 0.032
2.777 ± 0.032
10.337 ± 0.063
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.204 ± 0.019
2.77 ± 0.026
5.364 ± 0.038
3.468 ± 0.026
6.419 ± 0.046
5.874 ± 0.029
5.904 ± 0.035
7.804 ± 0.041
1.473 ± 0.02
2.1 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here