Fowl aviadenovirus 4
Average proteome isoelectric point is 6.82
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 42 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1B1SLH1|A0A1B1SLH1_9ADEN ORF2 OS=Fowl aviadenovirus 4 OX=130663 PE=4 SV=1
MM1 pKa = 8.07 AEE3 pKa = 3.44 EE4 pKa = 4.28 WLDD7 pKa = 3.66 LFHH10 pKa = 7.59 PSTSPNPEE18 pKa = 3.77 GEE20 pKa = 4.37 GEE22 pKa = 4.17 DD23 pKa = 3.38 MSLEE27 pKa = 4.49 TEE29 pKa = 4.17 CHH31 pKa = 6.44 APLQYY36 pKa = 10.85 ISMLSFDD43 pKa = 5.25 DD44 pKa = 4.26 LLAAAGPPDD53 pKa = 3.92 YY54 pKa = 11.09 SPEE57 pKa = 4.18 EE58 pKa = 4.06 NQEE61 pKa = 4.0 TPPIEE66 pKa = 4.37 TIEE69 pKa = 4.24 VGDD72 pKa = 3.65 IMAEE76 pKa = 3.72 LGIPIEE82 pKa = 4.72 GPPTSPSDD90 pKa = 3.52 SSSSLDD96 pKa = 3.3 SVLFSGVDD104 pKa = 3.83 LYY106 pKa = 11.61 DD107 pKa = 3.97 LDD109 pKa = 3.96 YY110 pKa = 10.96 TIVFSRR116 pKa = 11.84 LRR118 pKa = 11.84 EE119 pKa = 3.9 FWQSHH124 pKa = 4.12 GAYY127 pKa = 9.28 LKK129 pKa = 9.53 TVASLEE135 pKa = 4.04 CMQNDD140 pKa = 3.53 RR141 pKa = 11.84 KK142 pKa = 10.15 FQEE145 pKa = 4.95 AYY147 pKa = 9.4 CSLVRR152 pKa = 11.84 MHH154 pKa = 6.99 AVSEE158 pKa = 4.2 DD159 pKa = 3.47 AKK161 pKa = 10.72 EE162 pKa = 4.03 HH163 pKa = 6.31 LNEE166 pKa = 4.65 LLLDD170 pKa = 3.62 EE171 pKa = 5.09 TNYY174 pKa = 8.96 QHH176 pKa = 7.46 CEE178 pKa = 3.96 PLNDD182 pKa = 3.65 MLDD185 pKa = 3.71 LGFRR189 pKa = 11.84 WLNDD193 pKa = 3.33 LKK195 pKa = 11.03 GGIMEE200 pKa = 4.55 WCMDD204 pKa = 3.75 TALDD208 pKa = 3.88 RR209 pKa = 11.84 ASKK212 pKa = 10.65 VMPLTDD218 pKa = 3.67 YY219 pKa = 11.04 QPQQ222 pKa = 3.17
Molecular weight: 25.03 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.767
IPC2_protein 3.986
IPC_protein 3.961
Toseland 3.77
ProMoST 4.088
Dawson 3.923
Bjellqvist 4.075
Wikipedia 3.821
Rodwell 3.783
Grimsley 3.681
Solomon 3.923
Lehninger 3.872
Nozaki 4.037
DTASelect 4.215
Thurlkill 3.795
EMBOSS 3.834
Sillero 4.075
Patrickios 1.24
IPC_peptide 3.923
IPC2_peptide 4.062
IPC2.peptide.svr19 3.959
Protein with the highest isoelectric point:
>tr|A0A1B1SLF0|A0A1B1SLF0_9ADEN ORF13 OS=Fowl aviadenovirus 4 OX=130663 PE=4 SV=1
MM1 pKa = 7.68 SILISPNNNTGWGMRR16 pKa = 11.84 RR17 pKa = 11.84 RR18 pKa = 11.84 SRR20 pKa = 11.84 SSSMRR25 pKa = 11.84 GVGMRR30 pKa = 11.84 RR31 pKa = 11.84 RR32 pKa = 11.84 ARR34 pKa = 11.84 PLTLRR39 pKa = 11.84 SLLGLGTRR47 pKa = 11.84 RR48 pKa = 11.84 RR49 pKa = 11.84 RR50 pKa = 11.84 GSRR53 pKa = 11.84 RR54 pKa = 11.84 SRR56 pKa = 11.84 PRR58 pKa = 11.84 TTSRR62 pKa = 11.84 LVVVRR67 pKa = 11.84 TRR69 pKa = 11.84 TSSMRR74 pKa = 11.84 RR75 pKa = 11.84 RR76 pKa = 11.84 RR77 pKa = 3.5
Molecular weight: 9.04 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.454
IPC2_protein 11.608
IPC_protein 13.217
Toseland 13.378
ProMoST 13.876
Dawson 13.378
Bjellqvist 13.378
Wikipedia 13.861
Rodwell 12.881
Grimsley 13.422
Solomon 13.876
Lehninger 13.773
Nozaki 13.378
DTASelect 13.378
Thurlkill 13.378
EMBOSS 13.876
Sillero 13.378
Patrickios 12.603
IPC_peptide 13.89
IPC2_peptide 12.866
IPC2.peptide.svr19 9.484
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
41
1
42
13042
48
1251
310.5
34.95
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.476 ± 0.392
1.902 ± 0.237
5.321 ± 0.277
5.743 ± 0.419
4.263 ± 0.228
6.364 ± 0.344
2.408 ± 0.226
4.209 ± 0.222
3.665 ± 0.293
8.887 ± 0.315
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.645 ± 0.179
4.301 ± 0.43
6.878 ± 0.282
4.033 ± 0.246
7.0 ± 0.506
6.87 ± 0.325
6.533 ± 0.587
6.057 ± 0.302
1.372 ± 0.109
4.071 ± 0.271
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here