Caenorhabditis latens
Average proteome isoelectric point is 6.7
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 25394 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A261BFL7|A0A261BFL7_9PELO Uncharacterized protein (Fragment) OS=Caenorhabditis latens OX=1503980 GN=FL83_16552 PE=4 SV=1
MM1 pKa = 6.96 NQNTYY6 pKa = 10.71 SADD9 pKa = 3.51 SRR11 pKa = 11.84 GGTINYY17 pKa = 7.58 TVTNICNFVVIPHH30 pKa = 6.4 SFAAPPFCPPSGNPPTSNYY49 pKa = 8.81 HH50 pKa = 4.67 QNEE53 pKa = 4.09 GASRR57 pKa = 11.84 SRR59 pKa = 11.84 GDD61 pKa = 3.45 GEE63 pKa = 4.79 NDD65 pKa = 3.32 YY66 pKa = 11.71 GDD68 pKa = 4.4 AGAPNTPSNDD78 pKa = 3.23 NTDD81 pKa = 4.07 PEE83 pKa = 4.36 GHH85 pKa = 6.72 PVDD88 pKa = 5.26 GIRR91 pKa = 11.84 ITGMPTALIALPYY104 pKa = 9.2 LDD106 pKa = 4.95 PMVDD110 pKa = 3.29 STRR113 pKa = 11.84 LVAIAAEE120 pKa = 4.2 NRR122 pKa = 11.84 SPLTTDD128 pKa = 4.58 ALITSNNPSSVATHH142 pKa = 5.92 TEE144 pKa = 3.71 VSDD147 pKa = 3.66 AEE149 pKa = 4.24 RR150 pKa = 11.84 NIAEE154 pKa = 4.22 YY155 pKa = 9.85 TGEE158 pKa = 4.04 SDD160 pKa = 4.43 EE161 pKa = 4.79 EE162 pKa = 4.6 PNTAEE167 pKa = 4.0 PAGTHH172 pKa = 3.89 STEE175 pKa = 4.19 YY176 pKa = 8.98 EE177 pKa = 3.91 TTNAVTAEE185 pKa = 4.11 TANLEE190 pKa = 4.24 RR191 pKa = 11.84 SDD193 pKa = 3.41 TAAAAEE199 pKa = 4.27 SGVIQTVDD207 pKa = 3.97 FEE209 pKa = 4.63 SADD212 pKa = 3.47 AEE214 pKa = 4.64 TYY216 pKa = 10.69 SVAEE220 pKa = 4.18 TAEE223 pKa = 4.47 TPTSDD228 pKa = 5.13 AEE230 pKa = 4.37 TADD233 pKa = 3.69 TQLVDD238 pKa = 3.88 VRR240 pKa = 11.84 MDD242 pKa = 4.03 DD243 pKa = 5.55 DD244 pKa = 3.8 EE245 pKa = 4.47 TADD248 pKa = 3.82 AVIAGYY254 pKa = 10.18 QLTDD258 pKa = 4.63 DD259 pKa = 5.13 PDD261 pKa = 6.04 ADD263 pKa = 4.32 TPNADD268 pKa = 3.6 PQVTSVQMNHH278 pKa = 6.14 TPDD281 pKa = 3.69 ADD283 pKa = 3.53 AGLIDD288 pKa = 4.2 FQLDD292 pKa = 3.66 DD293 pKa = 3.62 AHH295 pKa = 6.62 MEE297 pKa = 4.03 QNLDD301 pKa = 3.49 VVADD305 pKa = 3.99 GLEE308 pKa = 4.21 TEE310 pKa = 4.66 SAISGALLFNN320 pKa = 4.92
Molecular weight: 33.7 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.714
IPC2_protein 3.617
IPC_protein 3.63
Toseland 3.414
ProMoST 3.795
Dawson 3.63
Bjellqvist 3.783
Wikipedia 3.554
Rodwell 3.465
Grimsley 3.325
Solomon 3.617
Lehninger 3.579
Nozaki 3.745
DTASelect 3.973
Thurlkill 3.465
EMBOSS 3.567
Sillero 3.757
Patrickios 1.202
IPC_peptide 3.617
IPC2_peptide 3.732
IPC2.peptide.svr19 3.708
Protein with the highest isoelectric point:
>tr|A0A261CEL6|A0A261CEL6_9PELO Protein kinase domain-containing protein (Fragment) OS=Caenorhabditis latens OX=1503980 GN=FL83_04616 PE=4 SV=1
MM1 pKa = 7.24 TRR3 pKa = 11.84 GTAPRR8 pKa = 11.84 HH9 pKa = 5.14 RR10 pKa = 11.84 PPRR13 pKa = 11.84 PATPRR18 pKa = 11.84 QPPAAIVHH26 pKa = 6.45 HH27 pKa = 6.39 SPPNNSTFSRR37 pKa = 11.84 HH38 pKa = 4.01 RR39 pKa = 11.84 SVRR42 pKa = 11.84 RR43 pKa = 11.84 RR44 pKa = 11.84 IQMGRR49 pKa = 11.84 RR50 pKa = 11.84 GRR52 pKa = 11.84 TSPRR56 pKa = 11.84 RR57 pKa = 11.84 PDD59 pKa = 3.22 SPRR62 pKa = 11.84 TKK64 pKa = 10.61 LL65 pKa = 3.37
Molecular weight: 7.49 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.486
IPC2_protein 11.067
IPC_protein 12.691
Toseland 12.852
ProMoST 13.349
Dawson 12.852
Bjellqvist 12.852
Wikipedia 13.32
Rodwell 12.369
Grimsley 12.881
Solomon 13.349
Lehninger 13.247
Nozaki 12.852
DTASelect 12.852
Thurlkill 12.852
EMBOSS 13.349
Sillero 12.852
Patrickios 12.106
IPC_peptide 13.349
IPC2_peptide 12.34
IPC2.peptide.svr19 9.159
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
24651
743
25394
9829288
17
7710
387.1
43.78
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.056 ± 0.018
1.946 ± 0.013
5.421 ± 0.015
7.061 ± 0.026
4.535 ± 0.017
5.266 ± 0.019
2.316 ± 0.009
5.985 ± 0.015
6.508 ± 0.021
8.469 ± 0.021
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.652 ± 0.007
4.887 ± 0.011
5.014 ± 0.019
4.074 ± 0.016
5.359 ± 0.016
7.979 ± 0.02
5.956 ± 0.021
6.254 ± 0.016
1.112 ± 0.005
3.121 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here