archaeon HR03

Taxonomy: cellular organisms; Archaea; unclassified Archaea

Average proteome isoelectric point is 7.0

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 434 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2H5V6V6|A0A2H5V6V6_9ARCH Isopentenyl phosphate kinase OS=archaeon HR03 OX=2035439 GN=argB PE=3 SV=1
MM1 pKa = 7.4VSPDD5 pKa = 3.41YY6 pKa = 10.93FFAEE10 pKa = 4.16IFGMCCEE17 pKa = 4.03QFADD21 pKa = 4.01DD22 pKa = 4.31VEE24 pKa = 4.71VDD26 pKa = 3.73VFPLGVPHH34 pKa = 7.45RR35 pKa = 11.84FLASQCDD42 pKa = 3.53PALVNLFNLVITLYY56 pKa = 9.6QRR58 pKa = 11.84RR59 pKa = 11.84II60 pKa = 3.47

Molecular weight: 6.9 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2H5V6H6|A0A2H5V6H6_9ARCH Leucine-responsive regulatory protein OS=archaeon HR03 OX=2035439 GN=lrp PE=4 SV=1
MM1 pKa = 7.79AMVPMLKK8 pKa = 10.23NITSAAISNVVKK20 pKa = 10.67KK21 pKa = 10.25VANLLRR27 pKa = 11.84VRR29 pKa = 11.84RR30 pKa = 11.84YY31 pKa = 10.69AMRR34 pKa = 11.84GMSATAAEE42 pKa = 4.42EE43 pKa = 3.82RR44 pKa = 11.84RR45 pKa = 11.84EE46 pKa = 4.46GISRR50 pKa = 11.84QPTGLSPKK58 pKa = 9.6TSIEE62 pKa = 3.74AAISSFEE69 pKa = 4.22RR70 pKa = 11.84GGCSWFTDD78 pKa = 3.43SPLNSFLTAGTT89 pKa = 3.72

Molecular weight: 9.59 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

434

0

434

110130

32

1254

253.8

28.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.446 ± 0.117

0.872 ± 0.047

4.449 ± 0.077

7.212 ± 0.125

4.023 ± 0.087

7.625 ± 0.104

1.802 ± 0.056

5.93 ± 0.1

5.526 ± 0.107

10.54 ± 0.139

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.558 ± 0.056

2.956 ± 0.066

4.695 ± 0.063

2.415 ± 0.051

6.361 ± 0.098

5.727 ± 0.098

5.094 ± 0.08

9.231 ± 0.115

1.124 ± 0.057

3.413 ± 0.064
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski