Cyclovirus Chimp11
Average proteome isoelectric point is 8.74
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|D4N3P7|D4N3P7_9CIRC Capsid protein OS=Cyclovirus Chimp11 OX=742920 GN=cap PE=4 SV=1
MM1 pKa = 7.71 SNRR4 pKa = 11.84 TVRR7 pKa = 11.84 KK8 pKa = 9.49 FCFTWNNYY16 pKa = 8.61 EE17 pKa = 3.44 FDD19 pKa = 4.46 AYY21 pKa = 10.49 AKK23 pKa = 10.9 CEE25 pKa = 3.93 TFLNNFAKK33 pKa = 10.57 YY34 pKa = 10.03 GIVGEE39 pKa = 4.34 EE40 pKa = 4.3 LCPSTGTPHH49 pKa = 6.18 LQGYY53 pKa = 9.24 VNLIKK58 pKa = 9.37 PTRR61 pKa = 11.84 FSTIKK66 pKa = 10.19 KK67 pKa = 8.11 HH68 pKa = 5.11 LHH70 pKa = 4.87 NAIHH74 pKa = 6.47 IEE76 pKa = 3.94 KK77 pKa = 10.73 ANGSDD82 pKa = 3.6 EE83 pKa = 4.48 QNQTYY88 pKa = 9.65 CRR90 pKa = 11.84 KK91 pKa = 10.23 SGIFFEE97 pKa = 4.68 KK98 pKa = 10.77 GEE100 pKa = 4.58 PIKK103 pKa = 10.55 QGQRR107 pKa = 11.84 TDD109 pKa = 3.27 LQLLVKK115 pKa = 10.11 DD116 pKa = 3.73 TMEE119 pKa = 4.65 PSNTLKK125 pKa = 10.83 DD126 pKa = 3.28 IATKK130 pKa = 10.65 HH131 pKa = 5.76 PIAYY135 pKa = 8.54 IRR137 pKa = 11.84 YY138 pKa = 7.9 FRR140 pKa = 11.84 GIQEE144 pKa = 4.4 LRR146 pKa = 11.84 RR147 pKa = 11.84 MVLPVPPRR155 pKa = 11.84 NYY157 pKa = 7.83 PTEE160 pKa = 3.67 VRR162 pKa = 11.84 YY163 pKa = 10.06 YY164 pKa = 9.21 WGPPGSGKK172 pKa = 8.76 SRR174 pKa = 11.84 RR175 pKa = 11.84 ALQEE179 pKa = 3.42 ATEE182 pKa = 4.05 ISTNGIYY189 pKa = 10.21 YY190 pKa = 10.12 KK191 pKa = 10.53 PRR193 pKa = 11.84 GQWWDD198 pKa = 3.42 GYY200 pKa = 7.55 EE201 pKa = 3.96 QQSCVIIDD209 pKa = 4.9 DD210 pKa = 4.45 FYY212 pKa = 11.76 GWIKK216 pKa = 10.51 YY217 pKa = 10.15 DD218 pKa = 4.69 EE219 pKa = 4.23 ILKK222 pKa = 10.17 ICDD225 pKa = 3.22 RR226 pKa = 11.84 YY227 pKa = 9.85 PYY229 pKa = 10.1 KK230 pKa = 10.91 VQIKK234 pKa = 10.07 GGYY237 pKa = 8.28 EE238 pKa = 4.0 EE239 pKa = 4.65 FTSTHH244 pKa = 4.68 IWFTSNVDD252 pKa = 2.84 TDD254 pKa = 4.26 LLYY257 pKa = 11.15 KK258 pKa = 10.08 FNNYY262 pKa = 9.13 INTAFEE268 pKa = 3.72 RR269 pKa = 11.84 RR270 pKa = 11.84 ITIKK274 pKa = 10.45 EE275 pKa = 4.27 HH276 pKa = 5.24 ITLSS280 pKa = 3.52
Molecular weight: 32.95 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.159
IPC2_protein 8.214
IPC_protein 8.112
Toseland 8.273
ProMoST 8.551
Dawson 8.843
Bjellqvist 8.931
Wikipedia 8.931
Rodwell 8.902
Grimsley 8.404
Solomon 8.96
Lehninger 8.916
Nozaki 8.975
DTASelect 8.77
Thurlkill 8.814
EMBOSS 8.99
Sillero 9.048
Patrickios 4.037
IPC_peptide 8.946
IPC2_peptide 7.775
IPC2.peptide.svr19 7.79
Protein with the highest isoelectric point:
>tr|D4N3P7|D4N3P7_9CIRC Capsid protein OS=Cyclovirus Chimp11 OX=742920 GN=cap PE=4 SV=1
MM1 pKa = 7.47 AFKK4 pKa = 10.5 RR5 pKa = 11.84 YY6 pKa = 7.66 FRR8 pKa = 11.84 RR9 pKa = 11.84 RR10 pKa = 11.84 RR11 pKa = 11.84 RR12 pKa = 11.84 VRR14 pKa = 11.84 KK15 pKa = 7.51 PVRR18 pKa = 11.84 RR19 pKa = 11.84 FRR21 pKa = 11.84 RR22 pKa = 11.84 RR23 pKa = 11.84 YY24 pKa = 7.92 RR25 pKa = 11.84 MRR27 pKa = 11.84 RR28 pKa = 11.84 RR29 pKa = 11.84 VLRR32 pKa = 11.84 SKK34 pKa = 9.88 PGNMLTKK41 pKa = 9.51 LTKK44 pKa = 9.08 ITTLSVEE51 pKa = 4.15 NNINATWSCSFKK63 pKa = 10.68 MGDD66 pKa = 3.14 FTEE69 pKa = 4.29 YY70 pKa = 11.04 GRR72 pKa = 11.84 LAPNFEE78 pKa = 4.37 TVKK81 pKa = 10.39 LNKK84 pKa = 9.75 VVVRR88 pKa = 11.84 VQPLQNVANNSTSSVPAYY106 pKa = 10.49 VVVPWHH112 pKa = 5.92 YY113 pKa = 11.25 NIALPKK119 pKa = 10.63 DD120 pKa = 3.31 FASYY124 pKa = 11.01 LRR126 pKa = 11.84 IDD128 pKa = 3.44 KK129 pKa = 10.75 HH130 pKa = 6.08 KK131 pKa = 10.92 LRR133 pKa = 11.84 AQTVGTSMSFVPNIVTVGVANEE155 pKa = 4.2 GANPTGRR162 pKa = 11.84 NITWKK167 pKa = 8.77 PTLEE171 pKa = 4.07 CLGVDD176 pKa = 3.18 INIPRR181 pKa = 11.84 VYY183 pKa = 10.34 CGAICFQGQPDD194 pKa = 3.62 MEE196 pKa = 4.31 GRR198 pKa = 11.84 KK199 pKa = 7.26 TAFNIITDD207 pKa = 3.78 VYY209 pKa = 10.41 CTFRR213 pKa = 11.84 NQNTMKK219 pKa = 10.53 VV220 pKa = 3.37
Molecular weight: 25.42 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.318
IPC2_protein 9.97
IPC_protein 10.935
Toseland 11.008
ProMoST 10.921
Dawson 11.082
Bjellqvist 10.862
Wikipedia 11.359
Rodwell 11.228
Grimsley 11.125
Solomon 11.286
Lehninger 11.242
Nozaki 10.994
DTASelect 10.862
Thurlkill 11.008
EMBOSS 11.433
Sillero 11.023
Patrickios 10.935
IPC_peptide 11.301
IPC2_peptide 10.087
IPC2.peptide.svr19 8.404
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
500
220
280
250.0
29.18
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.4 ± 0.693
2.2 ± 0.048
3.6 ± 0.574
5.0 ± 1.495
5.0 ± 0.0
5.8 ± 0.526
1.8 ± 0.586
7.0 ± 1.315
7.0 ± 0.418
5.6 ± 0.096
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.0 ± 0.777
6.8 ± 0.909
5.2 ± 0.167
3.6 ± 0.574
8.4 ± 1.949
4.6 ± 0.036
8.0 ± 0.12
6.6 ± 2.535
1.8 ± 0.287
5.6 ± 1.291
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here