Proteiniphilum saccharofermentans
Average proteome isoelectric point is 6.25
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3381 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1R3T542|A0A1R3T542_9BACT Uncharacterized protein OS=Proteiniphilum saccharofermentans OX=1642647 GN=PSM36_2511 PE=4 SV=1
MM1 pKa = 7.38 MGTFYY6 pKa = 10.87 RR7 pKa = 11.84 KK8 pKa = 9.73 IMFLDD13 pKa = 3.61 DD14 pKa = 3.71 AMIVRR19 pKa = 11.84 PVTAEE24 pKa = 4.15 DD25 pKa = 3.47 IKK27 pKa = 10.47 WANNEE32 pKa = 4.36 LEE34 pKa = 4.13 NSNLPLIPTGYY45 pKa = 11.31 ADD47 pKa = 5.4 FLKK50 pKa = 10.33 HH51 pKa = 6.05 CNGVAFNGVEE61 pKa = 4.16 LYY63 pKa = 9.46 GTDD66 pKa = 3.78 IVTDD70 pKa = 3.72 PQTNFKK76 pKa = 10.77 LIDD79 pKa = 3.23 IVSFSEE85 pKa = 4.0 QQLEE89 pKa = 4.29 LFNDD93 pKa = 3.14 QLLYY97 pKa = 10.44 FGRR100 pKa = 11.84 VDD102 pKa = 4.89 DD103 pKa = 7.01 DD104 pKa = 3.75 IFTFNPAVEE113 pKa = 4.36 KK114 pKa = 11.39 YY115 pKa = 6.27 EE116 pKa = 4.09 TRR118 pKa = 11.84 DD119 pKa = 3.11 ITSFEE124 pKa = 3.37 IWNEE128 pKa = 3.79 YY129 pKa = 10.89 NSFEE133 pKa = 4.07 EE134 pKa = 4.32 FLEE137 pKa = 4.27 KK138 pKa = 10.42 EE139 pKa = 3.89 ISEE142 pKa = 4.46 KK143 pKa = 10.87 YY144 pKa = 10.71 LPTEE148 pKa = 3.89 EE149 pKa = 4.81 NEE151 pKa = 4.63 VISSMVYY158 pKa = 10.54 SRR160 pKa = 11.84 MKK162 pKa = 10.05 EE163 pKa = 3.84 DD164 pKa = 3.56 PVFAKK169 pKa = 10.82 NILEE173 pKa = 4.4 YY174 pKa = 8.61 YY175 pKa = 10.35 TLMADD180 pKa = 3.5 SGNAEE185 pKa = 4.03 AQYY188 pKa = 10.86 QLAMLLMYY196 pKa = 10.4 EE197 pKa = 4.35 GGVVEE202 pKa = 4.33 QDD204 pKa = 2.97 YY205 pKa = 9.09 DD206 pKa = 4.0 TGFDD210 pKa = 3.15 WLARR214 pKa = 11.84 AAEE217 pKa = 3.98 QGYY220 pKa = 7.9 PQAGLLYY227 pKa = 10.6 LEE229 pKa = 4.77 EE230 pKa = 4.64 TSTDD234 pKa = 3.22 DD235 pKa = 5.07 DD236 pKa = 3.57 GRR238 pKa = 11.84 YY239 pKa = 9.52 DD240 pKa = 3.02 AWVV243 pKa = 3.0
Molecular weight: 28.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.771
IPC2_protein 3.923
IPC_protein 3.91
Toseland 3.706
ProMoST 4.037
Dawson 3.872
Bjellqvist 4.024
Wikipedia 3.757
Rodwell 3.732
Grimsley 3.617
Solomon 3.859
Lehninger 3.821
Nozaki 3.973
DTASelect 4.151
Thurlkill 3.745
EMBOSS 3.783
Sillero 4.012
Patrickios 1.888
IPC_peptide 3.859
IPC2_peptide 3.999
IPC2.peptide.svr19 3.897
Protein with the highest isoelectric point:
>tr|A0A1R3TC74|A0A1R3TC74_9BACT Putative rhamnogalacturonyl hydrolase OS=Proteiniphilum saccharofermentans OX=1642647 GN=PSM36_3433 PE=4 SV=1
MM1 pKa = 7.76 RR2 pKa = 11.84 NFIRR6 pKa = 11.84 RR7 pKa = 11.84 IAILLSGSMQVPADD21 pKa = 3.7 FLLFSIADD29 pKa = 3.34 VRR31 pKa = 11.84 LQFFKK36 pKa = 11.15 VYY38 pKa = 10.17 FII40 pKa = 5.0
Molecular weight: 4.74 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.318
IPC2_protein 9.838
IPC_protein 10.965
Toseland 10.921
ProMoST 11.008
Dawson 10.994
Bjellqvist 10.833
Wikipedia 11.33
Rodwell 10.965
Grimsley 11.052
Solomon 11.272
Lehninger 11.213
Nozaki 10.891
DTASelect 10.833
Thurlkill 10.921
EMBOSS 11.359
Sillero 10.95
Patrickios 10.906
IPC_peptide 11.272
IPC2_peptide 10.043
IPC2.peptide.svr19 8.534
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3381
0
3381
1306952
24
2576
386.6
43.69
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.556 ± 0.037
0.834 ± 0.013
5.697 ± 0.03
6.684 ± 0.038
4.787 ± 0.026
7.05 ± 0.032
1.865 ± 0.017
7.314 ± 0.035
6.18 ± 0.036
8.979 ± 0.039
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.532 ± 0.019
5.38 ± 0.039
3.955 ± 0.024
3.417 ± 0.021
4.82 ± 0.025
6.369 ± 0.033
5.556 ± 0.027
6.269 ± 0.03
1.364 ± 0.018
4.391 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here