Ralstonia phage GP4

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Gervaisevirus; Ralstonia virus GP4

Average proteome isoelectric point is 6.77

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 83 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A345GU09|A0A345GU09_9CAUD Uncharacterized protein OS=Ralstonia phage GP4 OX=2282904 PE=4 SV=1
MM1 pKa = 7.53LGIVQKK7 pKa = 9.44TATEE11 pKa = 3.89QLDD14 pKa = 3.56YY15 pKa = 11.26DD16 pKa = 3.64IDD18 pKa = 3.85FARR21 pKa = 11.84WMPDD25 pKa = 2.51GDD27 pKa = 4.05VLQSAGVVITPDD39 pKa = 4.17DD40 pKa = 3.79GTLTSPAYY48 pKa = 9.76EE49 pKa = 3.73IDD51 pKa = 3.6GTVVKK56 pKa = 10.55VWLAGGTAGASYY68 pKa = 11.0NVDD71 pKa = 3.27VTVATAAGRR80 pKa = 11.84IKK82 pKa = 9.01EE83 pKa = 4.57TCFKK87 pKa = 10.53TRR89 pKa = 11.84VRR91 pKa = 11.84SCC93 pKa = 3.15

Molecular weight: 9.9 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A345GTW0|A0A345GTW0_9CAUD IQ calmodulin-binding domain-containing family protein OS=Ralstonia phage GP4 OX=2282904 PE=4 SV=1
MM1 pKa = 7.55RR2 pKa = 11.84AIVDD6 pKa = 3.83GVEE9 pKa = 3.91IQIDD13 pKa = 3.92EE14 pKa = 4.44SDD16 pKa = 3.69LHH18 pKa = 7.27FMSLYY23 pKa = 9.3SWRR26 pKa = 11.84VQQQGSKK33 pKa = 9.87RR34 pKa = 11.84YY35 pKa = 9.25VRR37 pKa = 11.84ASTTRR42 pKa = 11.84KK43 pKa = 8.82GKK45 pKa = 10.12RR46 pKa = 11.84VTIYY50 pKa = 10.1LHH52 pKa = 7.14RR53 pKa = 11.84LITDD57 pKa = 3.8AGKK60 pKa = 10.86GVIVDD65 pKa = 4.15HH66 pKa = 6.88MDD68 pKa = 3.57GDD70 pKa = 3.98TMNNQRR76 pKa = 11.84SNFRR80 pKa = 11.84LGDD83 pKa = 3.38HH84 pKa = 7.45TINARR89 pKa = 11.84NARR92 pKa = 11.84KK93 pKa = 9.77IMARR97 pKa = 11.84TTSSRR102 pKa = 11.84FRR104 pKa = 11.84GVMLRR109 pKa = 11.84KK110 pKa = 9.89GKK112 pKa = 8.39WAARR116 pKa = 11.84IRR118 pKa = 11.84DD119 pKa = 3.96GVKK122 pKa = 10.05KK123 pKa = 10.52SAIEE127 pKa = 3.75LGQFSRR133 pKa = 11.84EE134 pKa = 3.61EE135 pKa = 3.93EE136 pKa = 3.53AAYY139 pKa = 10.05AYY141 pKa = 10.99DD142 pKa = 3.67MASLRR147 pKa = 11.84IHH149 pKa = 6.77GEE151 pKa = 3.41HH152 pKa = 6.68GRR154 pKa = 11.84RR155 pKa = 11.84NFLPLVFF162 pKa = 5.27

Molecular weight: 18.67 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

83

0

83

18700

47

3224

225.3

24.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.471 ± 0.537

0.872 ± 0.177

6.112 ± 0.181

6.273 ± 0.276

2.925 ± 0.192

8.61 ± 0.413

1.995 ± 0.17

4.567 ± 0.33

4.513 ± 0.275

6.882 ± 0.2

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.663 ± 0.155

3.513 ± 0.22

4.797 ± 0.209

4.134 ± 0.197

7.278 ± 0.283

5.594 ± 0.252

5.594 ± 0.263

6.294 ± 0.167

1.406 ± 0.142

2.508 ± 0.114
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski