Amino acid dipepetide frequency for Ralstonia phage GP4

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
23.531AlaAla: 23.531 ± 2.277
1.123AlaCys: 1.123 ± 0.331
5.829AlaAsp: 5.829 ± 0.594
9.733AlaGlu: 9.733 ± 1.157
3.476AlaPhe: 3.476 ± 0.389
10.963AlaGly: 10.963 ± 1.471
2.834AlaHis: 2.834 ± 0.366
5.455AlaIle: 5.455 ± 0.666
5.134AlaLys: 5.134 ± 0.543
9.733AlaLeu: 9.733 ± 0.688
3.744AlaMet: 3.744 ± 0.516
4.439AlaAsn: 4.439 ± 0.726
7.541AlaPro: 7.541 ± 1.145
6.31AlaGln: 6.31 ± 0.536
8.931AlaArg: 8.931 ± 0.819
7.006AlaSer: 7.006 ± 0.746
6.257AlaThr: 6.257 ± 0.639
7.22AlaVal: 7.22 ± 0.745
1.925AlaTrp: 1.925 ± 0.331
3.476AlaTyr: 3.476 ± 0.439
0.0AlaXaa: 0.0 ± 0.0
Cys
1.016CysAla: 1.016 ± 0.28
0.214CysCys: 0.214 ± 0.108
0.535CysAsp: 0.535 ± 0.261
0.535CysGlu: 0.535 ± 0.303
0.374CysPhe: 0.374 ± 0.218
0.802CysGly: 0.802 ± 0.252
0.481CysHis: 0.481 ± 0.244
0.374CysIle: 0.374 ± 0.136
0.321CysLys: 0.321 ± 0.153
0.535CysLeu: 0.535 ± 0.222
0.267CysMet: 0.267 ± 0.146
0.321CysAsn: 0.321 ± 0.148
0.16CysPro: 0.16 ± 0.095
0.214CysGln: 0.214 ± 0.131
0.695CysArg: 0.695 ± 0.256
0.267CysSer: 0.267 ± 0.127
0.321CysThr: 0.321 ± 0.17
0.642CysVal: 0.642 ± 0.25
0.321CysTrp: 0.321 ± 0.135
0.321CysTyr: 0.321 ± 0.171
0.0CysXaa: 0.0 ± 0.0
Asp
8.45AspAla: 8.45 ± 0.803
0.535AspCys: 0.535 ± 0.219
4.064AspAsp: 4.064 ± 0.637
3.957AspGlu: 3.957 ± 0.588
1.658AspPhe: 1.658 ± 0.233
8.022AspGly: 8.022 ± 0.723
1.497AspHis: 1.497 ± 0.35
2.888AspIle: 2.888 ± 0.484
1.818AspLys: 1.818 ± 0.338
4.171AspLeu: 4.171 ± 0.503
1.23AspMet: 1.23 ± 0.284
1.818AspAsn: 1.818 ± 0.305
2.514AspPro: 2.514 ± 0.311
1.872AspGln: 1.872 ± 0.367
4.278AspArg: 4.278 ± 0.438
2.727AspSer: 2.727 ± 0.504
3.102AspThr: 3.102 ± 0.37
3.904AspVal: 3.904 ± 0.454
0.909AspTrp: 0.909 ± 0.201
1.711AspTyr: 1.711 ± 0.286
0.0AspXaa: 0.0 ± 0.0
Glu
7.968GluAla: 7.968 ± 0.797
0.374GluCys: 0.374 ± 0.16
3.904GluAsp: 3.904 ± 0.405
4.011GluGlu: 4.011 ± 0.54
2.3GluPhe: 2.3 ± 0.322
4.064GluGly: 4.064 ± 0.533
1.658GluHis: 1.658 ± 0.357
3.369GluIle: 3.369 ± 0.297
2.353GluLys: 2.353 ± 0.413
5.455GluLeu: 5.455 ± 0.628
1.979GluMet: 1.979 ± 0.401
2.62GluAsn: 2.62 ± 0.331
3.262GluPro: 3.262 ± 0.599
3.048GluGln: 3.048 ± 0.408
6.043GluArg: 6.043 ± 0.837
3.262GluSer: 3.262 ± 0.458
2.353GluThr: 2.353 ± 0.336
2.407GluVal: 2.407 ± 0.367
1.123GluTrp: 1.123 ± 0.352
1.177GluTyr: 1.177 ± 0.259
0.0GluXaa: 0.0 ± 0.0
Phe
2.781PheAla: 2.781 ± 0.379
0.428PheCys: 0.428 ± 0.163
2.353PheAsp: 2.353 ± 0.369
1.497PheGlu: 1.497 ± 0.321
0.963PhePhe: 0.963 ± 0.239
2.781PheGly: 2.781 ± 0.353
0.535PheHis: 0.535 ± 0.194
1.711PheIle: 1.711 ± 0.427
1.123PheLys: 1.123 ± 0.302
1.765PheLeu: 1.765 ± 0.348
0.695PheMet: 0.695 ± 0.234
1.283PheAsn: 1.283 ± 0.276
1.016PhePro: 1.016 ± 0.283
1.123PheGln: 1.123 ± 0.308
1.658PheArg: 1.658 ± 0.494
1.818PheSer: 1.818 ± 0.269
1.283PheThr: 1.283 ± 0.22
2.888PheVal: 2.888 ± 0.32
0.374PheTrp: 0.374 ± 0.128
1.177PheTyr: 1.177 ± 0.232
0.0PheXaa: 0.0 ± 0.0
Gly
11.605GlyAla: 11.605 ± 1.47
0.856GlyCys: 0.856 ± 0.291
5.829GlyAsp: 5.829 ± 0.598
5.722GlyGlu: 5.722 ± 0.496
1.711GlyPhe: 1.711 ± 0.241
8.45GlyGly: 8.45 ± 1.149
1.39GlyHis: 1.39 ± 0.282
3.53GlyIle: 3.53 ± 0.461
4.92GlyLys: 4.92 ± 0.639
5.187GlyLeu: 5.187 ± 0.584
2.139GlyMet: 2.139 ± 0.331
3.637GlyAsn: 3.637 ± 0.475
2.246GlyPro: 2.246 ± 0.328
3.69GlyGln: 3.69 ± 0.512
6.31GlyArg: 6.31 ± 0.718
4.171GlySer: 4.171 ± 0.605
5.829GlyThr: 5.829 ± 0.765
5.562GlyVal: 5.562 ± 0.632
0.963GlyTrp: 0.963 ± 0.183
2.353GlyTyr: 2.353 ± 0.389
0.0GlyXaa: 0.0 ± 0.0
His
2.193HisAla: 2.193 ± 0.3
0.321HisCys: 0.321 ± 0.174
1.444HisAsp: 1.444 ± 0.335
1.658HisGlu: 1.658 ± 0.22
0.749HisPhe: 0.749 ± 0.179
1.765HisGly: 1.765 ± 0.335
0.588HisHis: 0.588 ± 0.25
0.909HisIle: 0.909 ± 0.242
0.856HisLys: 0.856 ± 0.232
1.016HisLeu: 1.016 ± 0.281
0.374HisMet: 0.374 ± 0.167
0.642HisAsn: 0.642 ± 0.209
1.177HisPro: 1.177 ± 0.244
0.481HisGln: 0.481 ± 0.132
1.925HisArg: 1.925 ± 0.421
1.016HisSer: 1.016 ± 0.242
1.07HisThr: 1.07 ± 0.244
1.177HisVal: 1.177 ± 0.251
0.053HisTrp: 0.053 ± 0.053
0.535HisTyr: 0.535 ± 0.206
0.0HisXaa: 0.0 ± 0.0
Ile
5.936IleAla: 5.936 ± 0.639
0.16IleCys: 0.16 ± 0.143
3.048IleAsp: 3.048 ± 0.485
4.278IleGlu: 4.278 ± 0.665
1.016IlePhe: 1.016 ± 0.251
4.011IleGly: 4.011 ± 0.559
0.695IleHis: 0.695 ± 0.207
2.032IleIle: 2.032 ± 0.421
2.407IleLys: 2.407 ± 0.438
2.139IleLeu: 2.139 ± 0.311
1.123IleMet: 1.123 ± 0.279
1.872IleAsn: 1.872 ± 0.333
1.818IlePro: 1.818 ± 0.298
1.872IleGln: 1.872 ± 0.322
2.834IleArg: 2.834 ± 0.506
2.727IleSer: 2.727 ± 0.468
2.567IleThr: 2.567 ± 0.594
3.583IleVal: 3.583 ± 0.609
0.428IleTrp: 0.428 ± 0.158
1.123IleTyr: 1.123 ± 0.261
0.0IleXaa: 0.0 ± 0.0
Lys
6.31LysAla: 6.31 ± 0.704
0.374LysCys: 0.374 ± 0.155
2.353LysAsp: 2.353 ± 0.48
2.3LysGlu: 2.3 ± 0.434
1.123LysPhe: 1.123 ± 0.288
3.209LysGly: 3.209 ± 0.512
0.428LysHis: 0.428 ± 0.147
2.086LysIle: 2.086 ± 0.383
2.086LysLys: 2.086 ± 0.356
3.423LysLeu: 3.423 ± 0.466
1.337LysMet: 1.337 ± 0.222
1.39LysAsn: 1.39 ± 0.25
3.209LysPro: 3.209 ± 0.454
1.979LysGln: 1.979 ± 0.35
4.064LysArg: 4.064 ± 0.509
1.925LysSer: 1.925 ± 0.349
2.888LysThr: 2.888 ± 0.454
2.941LysVal: 2.941 ± 0.361
1.07LysTrp: 1.07 ± 0.281
0.642LysTyr: 0.642 ± 0.185
0.0LysXaa: 0.0 ± 0.0
Leu
7.808LeuAla: 7.808 ± 0.679
0.749LeuCys: 0.749 ± 0.248
5.294LeuAsp: 5.294 ± 0.542
3.316LeuGlu: 3.316 ± 0.433
1.872LeuPhe: 1.872 ± 0.292
6.15LeuGly: 6.15 ± 0.657
1.658LeuHis: 1.658 ± 0.284
3.209LeuIle: 3.209 ± 0.53
3.744LeuLys: 3.744 ± 0.546
4.332LeuLeu: 4.332 ± 0.582
1.497LeuMet: 1.497 ± 0.32
1.925LeuAsn: 1.925 ± 0.33
3.637LeuPro: 3.637 ± 0.702
2.407LeuGln: 2.407 ± 0.269
5.936LeuArg: 5.936 ± 0.524
4.813LeuSer: 4.813 ± 0.605
4.492LeuThr: 4.492 ± 0.394
3.85LeuVal: 3.85 ± 0.611
0.642LeuTrp: 0.642 ± 0.207
1.497LeuTyr: 1.497 ± 0.255
0.0LeuXaa: 0.0 ± 0.0
Met
2.781MetAla: 2.781 ± 0.474
0.16MetCys: 0.16 ± 0.094
1.283MetAsp: 1.283 ± 0.283
0.963MetGlu: 0.963 ± 0.259
0.856MetPhe: 0.856 ± 0.225
2.032MetGly: 2.032 ± 0.345
0.695MetHis: 0.695 ± 0.205
0.695MetIle: 0.695 ± 0.22
1.658MetLys: 1.658 ± 0.303
2.139MetLeu: 2.139 ± 0.386
0.963MetMet: 0.963 ± 0.18
0.749MetAsn: 0.749 ± 0.223
2.193MetPro: 2.193 ± 0.337
1.283MetGln: 1.283 ± 0.274
2.353MetArg: 2.353 ± 0.449
2.086MetSer: 2.086 ± 0.328
1.872MetThr: 1.872 ± 0.314
1.177MetVal: 1.177 ± 0.263
0.267MetTrp: 0.267 ± 0.128
0.428MetTyr: 0.428 ± 0.178
0.0MetXaa: 0.0 ± 0.0
Asn
4.92AsnAla: 4.92 ± 0.521
0.214AsnCys: 0.214 ± 0.134
1.872AsnAsp: 1.872 ± 0.266
1.658AsnGlu: 1.658 ± 0.284
0.856AsnPhe: 0.856 ± 0.26
4.011AsnGly: 4.011 ± 0.655
0.749AsnHis: 0.749 ± 0.193
1.551AsnIle: 1.551 ± 0.359
1.23AsnLys: 1.23 ± 0.276
2.086AsnLeu: 2.086 ± 0.395
1.177AsnMet: 1.177 ± 0.286
1.283AsnAsn: 1.283 ± 0.322
1.979AsnPro: 1.979 ± 0.362
1.337AsnGln: 1.337 ± 0.301
2.407AsnArg: 2.407 ± 0.367
1.604AsnSer: 1.604 ± 0.319
2.246AsnThr: 2.246 ± 0.417
2.674AsnVal: 2.674 ± 0.416
0.642AsnTrp: 0.642 ± 0.152
0.642AsnTyr: 0.642 ± 0.205
0.0AsnXaa: 0.0 ± 0.0
Pro
8.931ProAla: 8.931 ± 1.452
0.214ProCys: 0.214 ± 0.113
2.995ProAsp: 2.995 ± 0.315
3.369ProGlu: 3.369 ± 0.448
1.444ProPhe: 1.444 ± 0.338
3.957ProGly: 3.957 ± 0.624
0.535ProHis: 0.535 ± 0.206
1.765ProIle: 1.765 ± 0.403
2.139ProLys: 2.139 ± 0.407
2.941ProLeu: 2.941 ± 0.466
0.802ProMet: 0.802 ± 0.243
1.177ProAsn: 1.177 ± 0.261
1.925ProPro: 1.925 ± 0.375
1.925ProGln: 1.925 ± 0.413
2.941ProArg: 2.941 ± 0.434
3.423ProSer: 3.423 ± 0.63
3.209ProThr: 3.209 ± 0.398
2.781ProVal: 2.781 ± 0.445
0.428ProTrp: 0.428 ± 0.129
1.07ProTyr: 1.07 ± 0.253
0.0ProXaa: 0.0 ± 0.0
Gln
5.776GlnAla: 5.776 ± 0.621
0.214GlnCys: 0.214 ± 0.115
1.604GlnAsp: 1.604 ± 0.355
2.246GlnGlu: 2.246 ± 0.338
1.23GlnPhe: 1.23 ± 0.297
2.567GlnGly: 2.567 ± 0.455
0.856GlnHis: 0.856 ± 0.294
1.979GlnIle: 1.979 ± 0.356
1.658GlnLys: 1.658 ± 0.348
3.262GlnLeu: 3.262 ± 0.337
1.711GlnMet: 1.711 ± 0.349
1.23GlnAsn: 1.23 ± 0.261
1.925GlnPro: 1.925 ± 0.297
3.155GlnGln: 3.155 ± 0.624
2.674GlnArg: 2.674 ± 0.514
2.46GlnSer: 2.46 ± 0.402
2.032GlnThr: 2.032 ± 0.339
2.995GlnVal: 2.995 ± 0.394
0.695GlnTrp: 0.695 ± 0.196
1.07GlnTyr: 1.07 ± 0.297
0.0GlnXaa: 0.0 ± 0.0
Arg
9.305ArgAla: 9.305 ± 0.915
0.481ArgCys: 0.481 ± 0.175
4.867ArgAsp: 4.867 ± 0.628
4.813ArgGlu: 4.813 ± 0.677
3.102ArgPhe: 3.102 ± 0.55
4.76ArgGly: 4.76 ± 0.559
1.39ArgHis: 1.39 ± 0.261
4.385ArgIle: 4.385 ± 0.674
3.583ArgLys: 3.583 ± 0.492
5.615ArgLeu: 5.615 ± 0.508
2.407ArgMet: 2.407 ± 0.368
2.62ArgAsn: 2.62 ± 0.392
2.834ArgPro: 2.834 ± 0.456
2.995ArgGln: 2.995 ± 0.438
4.813ArgArg: 4.813 ± 0.931
3.53ArgSer: 3.53 ± 0.36
3.423ArgThr: 3.423 ± 0.399
4.599ArgVal: 4.599 ± 0.582
1.016ArgTrp: 1.016 ± 0.221
2.246ArgTyr: 2.246 ± 0.329
0.0ArgXaa: 0.0 ± 0.0
Ser
6.952SerAla: 6.952 ± 0.899
0.588SerCys: 0.588 ± 0.212
3.85SerAsp: 3.85 ± 0.393
2.727SerGlu: 2.727 ± 0.295
1.337SerPhe: 1.337 ± 0.3
5.615SerGly: 5.615 ± 0.688
1.337SerHis: 1.337 ± 0.304
2.727SerIle: 2.727 ± 0.411
3.102SerLys: 3.102 ± 0.342
3.744SerLeu: 3.744 ± 0.465
1.23SerMet: 1.23 ± 0.296
1.925SerAsn: 1.925 ± 0.343
2.086SerPro: 2.086 ± 0.299
2.567SerGln: 2.567 ± 0.374
3.102SerArg: 3.102 ± 0.403
2.941SerSer: 2.941 ± 0.429
3.797SerThr: 3.797 ± 0.471
3.744SerVal: 3.744 ± 0.543
1.123SerTrp: 1.123 ± 0.212
1.444SerTyr: 1.444 ± 0.223
0.0SerXaa: 0.0 ± 0.0
Thr
6.631ThrAla: 6.631 ± 0.718
0.267ThrCys: 0.267 ± 0.155
2.727ThrAsp: 2.727 ± 0.413
3.637ThrGlu: 3.637 ± 0.481
1.765ThrPhe: 1.765 ± 0.343
5.241ThrGly: 5.241 ± 0.669
1.23ThrHis: 1.23 ± 0.247
3.048ThrIle: 3.048 ± 0.438
2.781ThrLys: 2.781 ± 0.308
4.385ThrLeu: 4.385 ± 0.481
1.444ThrMet: 1.444 ± 0.278
1.818ThrAsn: 1.818 ± 0.433
3.209ThrPro: 3.209 ± 0.416
1.872ThrGln: 1.872 ± 0.341
3.369ThrArg: 3.369 ± 0.65
2.995ThrSer: 2.995 ± 0.533
3.102ThrThr: 3.102 ± 0.486
4.011ThrVal: 4.011 ± 0.438
0.802ThrTrp: 0.802 ± 0.262
1.604ThrTyr: 1.604 ± 0.268
0.0ThrXaa: 0.0 ± 0.0
Val
7.754ValAla: 7.754 ± 0.762
1.016ValCys: 1.016 ± 0.266
3.85ValAsp: 3.85 ± 0.524
4.171ValGlu: 4.171 ± 0.599
1.818ValPhe: 1.818 ± 0.318
4.546ValGly: 4.546 ± 0.488
0.909ValHis: 0.909 ± 0.239
2.3ValIle: 2.3 ± 0.453
2.888ValLys: 2.888 ± 0.504
3.262ValLeu: 3.262 ± 0.349
1.497ValMet: 1.497 ± 0.246
2.727ValAsn: 2.727 ± 0.33
4.011ValPro: 4.011 ± 0.534
2.139ValGln: 2.139 ± 0.389
4.706ValArg: 4.706 ± 0.543
4.599ValSer: 4.599 ± 0.487
4.064ValThr: 4.064 ± 0.435
4.171ValVal: 4.171 ± 0.526
0.802ValTrp: 0.802 ± 0.263
1.711ValTyr: 1.711 ± 0.242
0.0ValXaa: 0.0 ± 0.0
Trp
1.23TrpAla: 1.23 ± 0.294
0.16TrpCys: 0.16 ± 0.091
0.856TrpAsp: 0.856 ± 0.267
0.481TrpGlu: 0.481 ± 0.172
0.535TrpPhe: 0.535 ± 0.228
1.07TrpGly: 1.07 ± 0.233
0.214TrpHis: 0.214 ± 0.12
0.588TrpIle: 0.588 ± 0.18
0.588TrpLys: 0.588 ± 0.202
1.604TrpLeu: 1.604 ± 0.248
0.481TrpMet: 0.481 ± 0.14
0.588TrpAsn: 0.588 ± 0.182
0.535TrpPro: 0.535 ± 0.185
0.321TrpGln: 0.321 ± 0.142
1.551TrpArg: 1.551 ± 0.385
1.07TrpSer: 1.07 ± 0.266
0.749TrpThr: 0.749 ± 0.182
1.016TrpVal: 1.016 ± 0.248
0.16TrpTrp: 0.16 ± 0.099
0.267TrpTyr: 0.267 ± 0.121
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.834TyrAla: 2.834 ± 0.367
0.267TyrCys: 0.267 ± 0.125
2.567TyrAsp: 2.567 ± 0.282
1.658TyrGlu: 1.658 ± 0.224
1.07TyrPhe: 1.07 ± 0.261
2.086TyrGly: 2.086 ± 0.463
0.267TyrHis: 0.267 ± 0.119
1.07TyrIle: 1.07 ± 0.236
0.749TyrLys: 0.749 ± 0.175
2.032TyrLeu: 2.032 ± 0.311
0.481TyrMet: 0.481 ± 0.15
1.07TyrAsn: 1.07 ± 0.258
0.695TyrPro: 0.695 ± 0.165
0.749TyrGln: 0.749 ± 0.195
2.193TyrArg: 2.193 ± 0.307
1.497TyrSer: 1.497 ± 0.281
1.283TyrThr: 1.283 ± 0.281
1.604TyrVal: 1.604 ± 0.255
0.321TyrTrp: 0.321 ± 0.228
0.588TyrTyr: 0.588 ± 0.155
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 83 proteins (18700 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski