Cladosporium fulvum T-1 virus
Average proteome isoelectric point is 7.61
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|V9H131|V9H131_9VIRU Env homologue OS=Cladosporium fulvum T-1 virus OX=2052899 PE=4 SV=1
MM1 pKa = 8.17 LSMPWLLIQATNPQGWQPEE20 pKa = 4.32 FFYY23 pKa = 11.28 GDD25 pKa = 3.1 RR26 pKa = 11.84 VKK28 pKa = 11.0 FDD30 pKa = 3.21 TWVSQMDD37 pKa = 3.85 MYY39 pKa = 11.44 FLFNSMTEE47 pKa = 3.81 NLKK50 pKa = 10.66 PIFATTFLRR59 pKa = 11.84 GRR61 pKa = 11.84 AQHH64 pKa = 5.21 WVKK67 pKa = 10.62 PFLRR71 pKa = 11.84 KK72 pKa = 9.97 YY73 pKa = 10.45 LDD75 pKa = 3.84 SNGEE79 pKa = 4.06 DD80 pKa = 3.39 NADD83 pKa = 3.38 GVFKK87 pKa = 10.79 SYY89 pKa = 10.88 NHH91 pKa = 6.87 LKK93 pKa = 10.41 HH94 pKa = 6.36 AMKK97 pKa = 10.57 SVFGVSNEE105 pKa = 3.25 IATAVRR111 pKa = 11.84 VIQHH115 pKa = 6.34 LTQKK119 pKa = 9.77 TSTAEE124 pKa = 3.83 YY125 pKa = 9.06 AAKK128 pKa = 9.61 FQEE131 pKa = 4.32 YY132 pKa = 10.39 AQLTDD137 pKa = 3.33 WDD139 pKa = 4.52 DD140 pKa = 3.43 EE141 pKa = 4.44 ALQVMYY147 pKa = 10.66 RR148 pKa = 11.84 RR149 pKa = 11.84 GLKK152 pKa = 9.77 EE153 pKa = 3.81 HH154 pKa = 6.65 VKK156 pKa = 10.86 DD157 pKa = 3.4 EE158 pKa = 4.1 LMRR161 pKa = 11.84 DD162 pKa = 3.26 GRR164 pKa = 11.84 KK165 pKa = 8.96 IDD167 pKa = 3.52 GLGDD171 pKa = 4.0 LVQVTIDD178 pKa = 4.76 LDD180 pKa = 3.91 DD181 pKa = 4.05 KK182 pKa = 11.13 LYY184 pKa = 10.42 EE185 pKa = 3.95 RR186 pKa = 11.84 AMEE189 pKa = 3.78 RR190 pKa = 11.84 RR191 pKa = 11.84 YY192 pKa = 10.53 DD193 pKa = 3.71 SKK195 pKa = 11.56 VSGKK199 pKa = 10.29 AGYY202 pKa = 7.78 TPGYY206 pKa = 9.91 DD207 pKa = 3.09 NRR209 pKa = 11.84 NRR211 pKa = 11.84 GFNDD215 pKa = 3.18 NYY217 pKa = 10.99 NKK219 pKa = 10.5 PKK221 pKa = 10.45 DD222 pKa = 3.6 KK223 pKa = 10.2 PYY225 pKa = 10.73 YY226 pKa = 9.87 GPQPMEE232 pKa = 4.14 LDD234 pKa = 3.55 VTEE237 pKa = 4.57 KK238 pKa = 10.54 GRR240 pKa = 11.84 KK241 pKa = 7.98 IRR243 pKa = 11.84 NSKK246 pKa = 9.7 GNRR249 pKa = 11.84 RR250 pKa = 11.84 PPSSRR255 pKa = 11.84 EE256 pKa = 3.33 TRR258 pKa = 11.84 TCYY261 pKa = 10.61 GCGKK265 pKa = 8.5 PGHH268 pKa = 6.71 IARR271 pKa = 11.84 DD272 pKa = 3.75 CRR274 pKa = 11.84 GKK276 pKa = 10.62 NMVRR280 pKa = 11.84 RR281 pKa = 11.84 EE282 pKa = 4.3 QFNMMQRR289 pKa = 11.84 RR290 pKa = 11.84 TSKK293 pKa = 10.58 SEE295 pKa = 3.86 SSVEE299 pKa = 3.92 SLGDD303 pKa = 2.86 IDD305 pKa = 3.46 YY306 pKa = 9.14 TRR308 pKa = 11.84 RR309 pKa = 11.84 VEE311 pKa = 4.03 EE312 pKa = 4.03 RR313 pKa = 11.84 ASNGSTEE320 pKa = 4.1 PLLDD324 pKa = 3.99 PSQGRR329 pKa = 11.84 GGCHH333 pKa = 5.17 GQQMVVIPFEE343 pKa = 4.28 LQNQPRR349 pKa = 11.84 QFNMMARR356 pKa = 11.84 RR357 pKa = 11.84 VDD359 pKa = 3.66 YY360 pKa = 10.52 EE361 pKa = 3.9 IEE363 pKa = 4.08 SEE365 pKa = 4.12 SRR367 pKa = 11.84 NGHH370 pKa = 6.25 GSLHH374 pKa = 6.0 WRR376 pKa = 11.84 FCYY379 pKa = 9.85 QDD381 pKa = 3.16 SCQVHH386 pKa = 5.67 YY387 pKa = 10.16 SAKK390 pKa = 10.24 SGAGYY395 pKa = 9.43 WPQQPRR401 pKa = 11.84 GTLGATHH408 pKa = 6.71 RR409 pKa = 11.84 QDD411 pKa = 3.04 ATLQEE416 pKa = 3.85 VDD418 pKa = 3.43 IDD420 pKa = 4.13 EE421 pKa = 5.01 SCFDD425 pKa = 4.93 DD426 pKa = 5.8 DD427 pKa = 6.69 GSDD430 pKa = 5.45 KK431 pKa = 11.14 EE432 pKa = 4.35 NQDD435 pKa = 3.84 PGWNGSWSPEE445 pKa = 3.88 PQEE448 pKa = 4.48 EE449 pKa = 4.43 SQEE452 pKa = 4.3 TTGLDD457 pKa = 3.74 TIEE460 pKa = 4.58 EE461 pKa = 4.27 DD462 pKa = 3.5 QDD464 pKa = 3.76 PEE466 pKa = 4.12 EE467 pKa = 4.65 SSEE470 pKa = 3.97 EE471 pKa = 3.92 DD472 pKa = 3.12 SSEE475 pKa = 4.22 GEE477 pKa = 4.34 EE478 pKa = 5.53 SDD480 pKa = 3.96 TDD482 pKa = 4.04 DD483 pKa = 6.21 DD484 pKa = 4.36 NEE486 pKa = 4.23 QMTFTVDD493 pKa = 3.36 APKK496 pKa = 10.59 KK497 pKa = 8.77 LYY499 pKa = 11.2 DD500 pKa = 4.13 MIIHH504 pKa = 6.22 LQRR507 pKa = 11.84 RR508 pKa = 11.84 HH509 pKa = 5.74 EE510 pKa = 4.19 EE511 pKa = 3.62 FLPRR515 pKa = 11.84 IGGRR519 pKa = 11.84 RR520 pKa = 11.84 MLHH523 pKa = 6.16 SIEE526 pKa = 4.04 FDD528 pKa = 3.1 KK529 pKa = 11.43 TLDD532 pKa = 3.66 TLRR535 pKa = 11.84 GMAWGYY541 pKa = 9.9 PLMEE545 pKa = 4.29 TTEE548 pKa = 4.16 NLATAVTEE556 pKa = 4.07 RR557 pKa = 11.84 PPIGSRR563 pKa = 11.84 MIGTGYY569 pKa = 8.29 LTPSGTFVSNEE580 pKa = 3.65 LRR582 pKa = 11.84 NMVQHH587 pKa = 6.69 ARR589 pKa = 11.84 ALYY592 pKa = 10.68 SEE594 pKa = 4.29 TQKK597 pKa = 9.91 IQEE600 pKa = 4.04 RR601 pKa = 11.84 HH602 pKa = 4.92 RR603 pKa = 11.84 VQMWTQKK610 pKa = 10.38 GSRR613 pKa = 11.84 QVQAEE618 pKa = 4.13 ASRR621 pKa = 11.84 HH622 pKa = 4.06 EE623 pKa = 4.4 ARR625 pKa = 11.84 RR626 pKa = 11.84 STYY629 pKa = 9.34 EE630 pKa = 3.56 QGKK633 pKa = 10.29 GSTPQEE639 pKa = 3.88
Molecular weight: 73.65 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.464
IPC2_protein 5.436
IPC_protein 5.448
Toseland 5.677
ProMoST 5.639
Dawson 5.575
Bjellqvist 5.652
Wikipedia 5.525
Rodwell 5.537
Grimsley 5.753
Solomon 5.575
Lehninger 5.55
Nozaki 5.779
DTASelect 5.944
Thurlkill 5.817
EMBOSS 5.779
Sillero 5.868
Patrickios 4.253
IPC_peptide 5.588
IPC2_peptide 5.868
IPC2.peptide.svr19 5.835
Protein with the highest isoelectric point:
>tr|V9H1L3|V9H1L3_9VIRU Reverse transcriptase (Fragment) OS=Cladosporium fulvum T-1 virus OX=2052899 PE=4 SV=1
MM1 pKa = 7.87 APLLKK6 pKa = 10.72 EE7 pKa = 3.74 GDD9 pKa = 3.63 KK10 pKa = 11.25 VYY12 pKa = 11.28 LLTKK16 pKa = 9.71 NLKK19 pKa = 7.89 TRR21 pKa = 11.84 RR22 pKa = 11.84 QTKK25 pKa = 10.16 KK26 pKa = 9.92 LDD28 pKa = 3.57 HH29 pKa = 6.52 VKK31 pKa = 10.55 VGPFFIDD38 pKa = 3.4 KK39 pKa = 10.7 VVGPVNYY46 pKa = 10.12 RR47 pKa = 11.84 LRR49 pKa = 11.84 LPPDD53 pKa = 3.52 AKK55 pKa = 9.92 IHH57 pKa = 5.45 PVFHH61 pKa = 6.85 ISKK64 pKa = 10.34 LEE66 pKa = 4.02 PADD69 pKa = 4.78 AEE71 pKa = 4.87 TPCQEE76 pKa = 4.23 SFHH79 pKa = 6.66 FEE81 pKa = 3.82 PEE83 pKa = 3.89 AEE85 pKa = 4.16 NEE87 pKa = 4.18 FEE89 pKa = 4.64 VEE91 pKa = 4.73 KK92 pKa = 10.95 ILDD95 pKa = 3.83 KK96 pKa = 10.96 KK97 pKa = 8.32 GQRR100 pKa = 11.84 YY101 pKa = 7.78 LVKK104 pKa = 9.91 WKK106 pKa = 10.71 GYY108 pKa = 10.27 DD109 pKa = 3.23 EE110 pKa = 5.74 SEE112 pKa = 4.41 NTWEE116 pKa = 4.61 PRR118 pKa = 11.84 INLANCYY125 pKa = 9.51 QLLRR129 pKa = 11.84 QFQKK133 pKa = 9.63 WRR135 pKa = 11.84 QDD137 pKa = 2.98 SRR139 pKa = 11.84 KK140 pKa = 9.57 QEE142 pKa = 3.92 AQEE145 pKa = 4.02 RR146 pKa = 11.84 RR147 pKa = 11.84 ASPDD151 pKa = 3.04 QTRR154 pKa = 11.84 SRR156 pKa = 11.84 PKK158 pKa = 9.87 YY159 pKa = 7.73 PHH161 pKa = 6.91 ARR163 pKa = 11.84 TKK165 pKa = 10.83
Molecular weight: 19.63 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.922
IPC2_protein 8.873
IPC_protein 8.785
Toseland 9.677
ProMoST 9.399
Dawson 9.882
Bjellqvist 9.531
Wikipedia 10.014
Rodwell 10.335
Grimsley 9.94
Solomon 9.926
Lehninger 9.897
Nozaki 9.721
DTASelect 9.516
Thurlkill 9.736
EMBOSS 10.087
Sillero 9.809
Patrickios 9.677
IPC_peptide 9.926
IPC2_peptide 8.039
IPC2.peptide.svr19 7.774
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
1849
165
1045
616.3
71.12
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.516 ± 0.452
1.19 ± 0.05
6.22 ± 0.583
7.734 ± 0.567
3.245 ± 0.228
5.895 ± 0.955
3.029 ± 0.2
4.543 ± 0.937
6.869 ± 1.166
7.247 ± 0.666
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.596 ± 0.633
3.678 ± 0.21
5.571 ± 0.565
5.192 ± 0.754
7.842 ± 0.323
6.057 ± 0.665
7.68 ± 1.252
4.489 ± 0.2
1.731 ± 0.018
3.678 ± 0.125
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here