Microbacterium phage Arroyo

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Dismasvirus; unclassified Dismasvirus

Average proteome isoelectric point is 6.23

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 69 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A514DIP2|A0A514DIP2_9CAUD Uncharacterized protein OS=Microbacterium phage Arroyo OX=2591213 GN=56 PE=4 SV=1
MM1 pKa = 7.48NNNEE5 pKa = 4.09TSILPHH11 pKa = 5.9VATMPVITKK20 pKa = 10.21VNLLAEE26 pKa = 5.46AILEE30 pKa = 4.32NKK32 pKa = 7.99GTVYY36 pKa = 9.67STANILVDD44 pKa = 4.62LVAIVCDD51 pKa = 3.42NAGFEE56 pKa = 4.27VEE58 pKa = 5.19LRR60 pKa = 11.84ITNEE64 pKa = 4.24GYY66 pKa = 10.63SIAFPQHH73 pKa = 5.97IEE75 pKa = 3.65ADD77 pKa = 4.01EE78 pKa = 4.22VFGALDD84 pKa = 3.08QGLEE88 pKa = 4.01VLEE91 pKa = 4.27EE92 pKa = 4.08MSLEE96 pKa = 4.2DD97 pKa = 3.89

Molecular weight: 10.58 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A514DIH5|A0A514DIH5_9CAUD Capsid maturation protease OS=Microbacterium phage Arroyo OX=2591213 GN=6 PE=4 SV=1
MM1 pKa = 7.7PGCPEE6 pKa = 3.42CRR8 pKa = 11.84ARR10 pKa = 11.84NRR12 pKa = 11.84DD13 pKa = 3.16SLFRR17 pKa = 11.84KK18 pKa = 9.23RR19 pKa = 11.84VEE21 pKa = 3.76RR22 pKa = 11.84GQLRR26 pKa = 11.84RR27 pKa = 11.84PGLVAEE33 pKa = 4.85RR34 pKa = 11.84AAMDD38 pKa = 3.78PAKK41 pKa = 10.33RR42 pKa = 11.84DD43 pKa = 3.56EE44 pKa = 4.59LDD46 pKa = 3.18RR47 pKa = 11.84ATRR50 pKa = 11.84NAGTVRR56 pKa = 11.84YY57 pKa = 8.86RR58 pKa = 11.84RR59 pKa = 11.84RR60 pKa = 11.84HH61 pKa = 5.05GRR63 pKa = 11.84PHH65 pKa = 6.26DD66 pKa = 3.4HH67 pKa = 7.51RR68 pKa = 11.84YY69 pKa = 8.71PGKK72 pKa = 8.77NTIALRR78 pKa = 11.84DD79 pKa = 3.28DD80 pKa = 3.3WLRR83 pKa = 11.84RR84 pKa = 11.84GIDD87 pKa = 3.47PDD89 pKa = 3.74TCFYY93 pKa = 11.05CPAPAEE99 pKa = 4.44HH100 pKa = 7.26ADD102 pKa = 3.49HH103 pKa = 6.42YY104 pKa = 10.74FPRR107 pKa = 11.84ARR109 pKa = 11.84GGSDD113 pKa = 3.09DD114 pKa = 4.4FKK116 pKa = 11.65NLVPACAPCNRR127 pKa = 11.84RR128 pKa = 11.84KK129 pKa = 9.98SDD131 pKa = 3.53RR132 pKa = 11.84LPIEE136 pKa = 3.6WLAIQTNSRR145 pKa = 11.84PPAAHH150 pKa = 5.56RR151 pKa = 11.84TVGG154 pKa = 3.2

Molecular weight: 17.72 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

69

0

69

13262

29

919

192.2

21.03

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.778 ± 0.406

0.475 ± 0.104

6.658 ± 0.315

5.934 ± 0.268

3.227 ± 0.157

8.181 ± 0.392

1.96 ± 0.186

4.645 ± 0.228

4.155 ± 0.24

8.709 ± 0.294

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.194 ± 0.152

3.386 ± 0.247

5.406 ± 0.215

3.348 ± 0.148

6.439 ± 0.376

5.21 ± 0.267

6.681 ± 0.433

7.005 ± 0.212

2.134 ± 0.155

2.473 ± 0.165
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski