Amino acid dipepetide frequency for Microbacterium phage Arroyo

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
13.423AlaAla: 13.423 ± 1.084
0.302AlaCys: 0.302 ± 0.15
6.862AlaAsp: 6.862 ± 0.7
7.315AlaGlu: 7.315 ± 0.782
3.77AlaPhe: 3.77 ± 0.448
11.613AlaGly: 11.613 ± 0.961
2.111AlaHis: 2.111 ± 0.41
6.41AlaIle: 6.41 ± 0.889
5.429AlaLys: 5.429 ± 0.751
9.954AlaLeu: 9.954 ± 1.051
2.715AlaMet: 2.715 ± 0.539
4.374AlaAsn: 4.374 ± 0.68
6.033AlaPro: 6.033 ± 0.736
5.807AlaGln: 5.807 ± 0.801
6.41AlaArg: 6.41 ± 0.813
5.128AlaSer: 5.128 ± 0.611
6.561AlaThr: 6.561 ± 1.007
7.918AlaVal: 7.918 ± 0.845
3.167AlaTrp: 3.167 ± 0.514
2.489AlaTyr: 2.489 ± 0.467
0.0AlaXaa: 0.0 ± 0.0
Cys
0.302CysAla: 0.302 ± 0.209
0.0CysCys: 0.0 ± 0.0
0.377CysAsp: 0.377 ± 0.169
0.151CysGlu: 0.151 ± 0.114
0.075CysPhe: 0.075 ± 0.073
0.679CysGly: 0.679 ± 0.282
0.302CysHis: 0.302 ± 0.168
0.075CysIle: 0.075 ± 0.071
0.075CysLys: 0.075 ± 0.071
0.151CysLeu: 0.151 ± 0.1
0.151CysMet: 0.151 ± 0.106
0.302CysAsn: 0.302 ± 0.158
0.905CysPro: 0.905 ± 0.304
0.151CysGln: 0.151 ± 0.092
0.302CysArg: 0.302 ± 0.181
0.226CysSer: 0.226 ± 0.117
0.226CysThr: 0.226 ± 0.126
0.226CysVal: 0.226 ± 0.138
0.0CysTrp: 0.0 ± 0.0
0.075CysTyr: 0.075 ± 0.081
0.0CysXaa: 0.0 ± 0.0
Asp
6.561AspAla: 6.561 ± 0.785
0.151AspCys: 0.151 ± 0.118
4.298AspAsp: 4.298 ± 0.658
3.921AspGlu: 3.921 ± 0.632
2.262AspPhe: 2.262 ± 0.332
6.485AspGly: 6.485 ± 0.643
2.036AspHis: 2.036 ± 0.46
2.941AspIle: 2.941 ± 0.373
1.659AspLys: 1.659 ± 0.308
6.636AspLeu: 6.636 ± 0.815
1.357AspMet: 1.357 ± 0.283
1.961AspAsn: 1.961 ± 0.303
4.826AspPro: 4.826 ± 0.612
2.111AspGln: 2.111 ± 0.297
3.62AspArg: 3.62 ± 0.674
3.469AspSer: 3.469 ± 0.499
3.469AspThr: 3.469 ± 0.479
4.449AspVal: 4.449 ± 0.61
1.659AspTrp: 1.659 ± 0.345
2.715AspTyr: 2.715 ± 0.336
0.0AspXaa: 0.0 ± 0.0
Glu
7.013GluAla: 7.013 ± 0.642
0.151GluCys: 0.151 ± 0.118
4.374GluAsp: 4.374 ± 0.604
3.997GluGlu: 3.997 ± 0.679
2.187GluPhe: 2.187 ± 0.327
3.846GluGly: 3.846 ± 0.679
1.961GluHis: 1.961 ± 0.367
1.131GluIle: 1.131 ± 0.247
2.413GluLys: 2.413 ± 0.45
6.485GluLeu: 6.485 ± 0.671
1.734GluMet: 1.734 ± 0.313
1.659GluAsn: 1.659 ± 0.424
2.866GluPro: 2.866 ± 0.591
2.338GluGln: 2.338 ± 0.402
3.167GluArg: 3.167 ± 0.453
2.036GluSer: 2.036 ± 0.455
3.469GluThr: 3.469 ± 0.456
5.656GluVal: 5.656 ± 0.636
1.508GluTrp: 1.508 ± 0.359
1.357GluTyr: 1.357 ± 0.34
0.0GluXaa: 0.0 ± 0.0
Phe
3.393PheAla: 3.393 ± 0.554
0.075PheCys: 0.075 ± 0.068
1.961PheAsp: 1.961 ± 0.313
2.866PheGlu: 2.866 ± 0.328
1.056PhePhe: 1.056 ± 0.291
3.092PheGly: 3.092 ± 0.519
0.452PheHis: 0.452 ± 0.168
1.433PheIle: 1.433 ± 0.368
1.961PheLys: 1.961 ± 0.306
2.187PheLeu: 2.187 ± 0.51
1.056PheMet: 1.056 ± 0.24
1.131PheAsn: 1.131 ± 0.264
1.584PhePro: 1.584 ± 0.303
0.905PheGln: 0.905 ± 0.263
3.318PheArg: 3.318 ± 0.514
1.81PheSer: 1.81 ± 0.351
1.81PheThr: 1.81 ± 0.364
1.056PheVal: 1.056 ± 0.266
0.528PheTrp: 0.528 ± 0.25
0.603PheTyr: 0.603 ± 0.2
0.0PheXaa: 0.0 ± 0.0
Gly
8.672GlyAla: 8.672 ± 1.096
0.528GlyCys: 0.528 ± 0.205
6.334GlyAsp: 6.334 ± 0.677
3.77GlyGlu: 3.77 ± 0.525
3.016GlyPhe: 3.016 ± 0.443
5.882GlyGly: 5.882 ± 0.884
1.433GlyHis: 1.433 ± 0.384
4.525GlyIle: 4.525 ± 0.68
4.148GlyLys: 4.148 ± 0.577
6.938GlyLeu: 6.938 ± 0.668
2.413GlyMet: 2.413 ± 0.546
2.639GlyAsn: 2.639 ± 0.599
2.79GlyPro: 2.79 ± 0.473
3.469GlyGln: 3.469 ± 0.68
4.902GlyArg: 4.902 ± 0.55
3.469GlySer: 3.469 ± 0.547
6.41GlyThr: 6.41 ± 1.276
5.203GlyVal: 5.203 ± 0.723
2.111GlyTrp: 2.111 ± 0.432
3.167GlyTyr: 3.167 ± 0.469
0.0GlyXaa: 0.0 ± 0.0
His
1.961HisAla: 1.961 ± 0.414
0.377HisCys: 0.377 ± 0.173
1.207HisAsp: 1.207 ± 0.288
0.679HisGlu: 0.679 ± 0.251
0.603HisPhe: 0.603 ± 0.228
1.81HisGly: 1.81 ± 0.356
0.679HisHis: 0.679 ± 0.284
0.754HisIle: 0.754 ± 0.278
0.83HisLys: 0.83 ± 0.277
2.262HisLeu: 2.262 ± 0.395
0.452HisMet: 0.452 ± 0.188
0.377HisAsn: 0.377 ± 0.166
0.98HisPro: 0.98 ± 0.28
0.377HisGln: 0.377 ± 0.165
1.659HisArg: 1.659 ± 0.509
1.056HisSer: 1.056 ± 0.279
0.905HisThr: 0.905 ± 0.313
1.508HisVal: 1.508 ± 0.415
0.075HisTrp: 0.075 ± 0.066
1.056HisTyr: 1.056 ± 0.268
0.0HisXaa: 0.0 ± 0.0
Ile
5.58IleAla: 5.58 ± 0.653
0.0IleCys: 0.0 ± 0.0
3.846IleAsp: 3.846 ± 0.474
3.544IleGlu: 3.544 ± 0.47
0.528IlePhe: 0.528 ± 0.196
2.639IleGly: 2.639 ± 0.487
1.056IleHis: 1.056 ± 0.31
2.489IleIle: 2.489 ± 0.419
2.111IleLys: 2.111 ± 0.587
4.148IleLeu: 4.148 ± 0.484
0.679IleMet: 0.679 ± 0.265
1.885IleAsn: 1.885 ± 0.473
2.715IlePro: 2.715 ± 0.55
1.357IleGln: 1.357 ± 0.281
3.318IleArg: 3.318 ± 0.482
1.584IleSer: 1.584 ± 0.355
3.921IleThr: 3.921 ± 0.656
3.167IleVal: 3.167 ± 0.552
0.679IleTrp: 0.679 ± 0.231
1.207IleTyr: 1.207 ± 0.281
0.0IleXaa: 0.0 ± 0.0
Lys
5.128LysAla: 5.128 ± 0.882
0.226LysCys: 0.226 ± 0.112
1.961LysAsp: 1.961 ± 0.48
2.111LysGlu: 2.111 ± 0.398
1.282LysPhe: 1.282 ± 0.343
2.79LysGly: 2.79 ± 0.434
0.302LysHis: 0.302 ± 0.139
1.885LysIle: 1.885 ± 0.469
1.961LysLys: 1.961 ± 0.483
3.544LysLeu: 3.544 ± 0.554
1.282LysMet: 1.282 ± 0.266
1.508LysAsn: 1.508 ± 0.349
3.695LysPro: 3.695 ± 0.599
1.282LysGln: 1.282 ± 0.33
3.318LysArg: 3.318 ± 0.489
2.262LysSer: 2.262 ± 0.49
2.489LysThr: 2.489 ± 0.424
3.393LysVal: 3.393 ± 0.458
0.377LysTrp: 0.377 ± 0.178
0.754LysTyr: 0.754 ± 0.196
0.0LysXaa: 0.0 ± 0.0
Leu
10.784LeuAla: 10.784 ± 1.076
0.377LeuCys: 0.377 ± 0.164
5.957LeuAsp: 5.957 ± 0.666
6.334LeuGlu: 6.334 ± 0.745
2.262LeuPhe: 2.262 ± 0.45
7.315LeuGly: 7.315 ± 0.718
0.83LeuHis: 0.83 ± 0.258
3.243LeuIle: 3.243 ± 0.493
4.298LeuLys: 4.298 ± 0.534
7.088LeuLeu: 7.088 ± 0.593
2.489LeuMet: 2.489 ± 0.345
2.715LeuAsn: 2.715 ± 0.528
4.6LeuPro: 4.6 ± 0.479
3.016LeuGln: 3.016 ± 0.456
5.957LeuArg: 5.957 ± 0.844
4.223LeuSer: 4.223 ± 0.638
6.184LeuThr: 6.184 ± 0.69
6.485LeuVal: 6.485 ± 0.825
1.207LeuTrp: 1.207 ± 0.268
1.734LeuTyr: 1.734 ± 0.347
0.0LeuXaa: 0.0 ± 0.0
Met
3.921MetAla: 3.921 ± 0.568
0.0MetCys: 0.0 ± 0.0
1.508MetAsp: 1.508 ± 0.348
0.905MetGlu: 0.905 ± 0.254
0.528MetPhe: 0.528 ± 0.205
1.357MetGly: 1.357 ± 0.252
0.0MetHis: 0.0 ± 0.0
0.679MetIle: 0.679 ± 0.273
1.357MetLys: 1.357 ± 0.406
1.207MetLeu: 1.207 ± 0.299
0.151MetMet: 0.151 ± 0.118
1.357MetAsn: 1.357 ± 0.31
1.433MetPro: 1.433 ± 0.379
0.302MetGln: 0.302 ± 0.156
0.905MetArg: 0.905 ± 0.314
1.584MetSer: 1.584 ± 0.418
2.262MetThr: 2.262 ± 0.405
1.508MetVal: 1.508 ± 0.353
0.603MetTrp: 0.603 ± 0.173
0.377MetTyr: 0.377 ± 0.152
0.0MetXaa: 0.0 ± 0.0
Asn
3.469AsnAla: 3.469 ± 0.628
0.151AsnCys: 0.151 ± 0.097
2.111AsnAsp: 2.111 ± 0.408
1.584AsnGlu: 1.584 ± 0.362
0.98AsnPhe: 0.98 ± 0.26
3.167AsnGly: 3.167 ± 0.499
0.603AsnHis: 0.603 ± 0.241
1.433AsnIle: 1.433 ± 0.333
1.131AsnLys: 1.131 ± 0.268
2.79AsnLeu: 2.79 ± 0.407
0.528AsnMet: 0.528 ± 0.204
1.81AsnAsn: 1.81 ± 0.352
2.489AsnPro: 2.489 ± 0.45
1.357AsnGln: 1.357 ± 0.274
1.81AsnArg: 1.81 ± 0.468
2.413AsnSer: 2.413 ± 0.43
2.338AsnThr: 2.338 ± 0.505
2.111AsnVal: 2.111 ± 0.516
0.679AsnTrp: 0.679 ± 0.272
0.905AsnTyr: 0.905 ± 0.236
0.0AsnXaa: 0.0 ± 0.0
Pro
8.37ProAla: 8.37 ± 0.733
0.528ProCys: 0.528 ± 0.216
3.469ProAsp: 3.469 ± 0.504
4.072ProGlu: 4.072 ± 0.733
1.885ProPhe: 1.885 ± 0.344
4.751ProGly: 4.751 ± 0.624
1.433ProHis: 1.433 ± 0.357
3.016ProIle: 3.016 ± 0.579
2.036ProLys: 2.036 ± 0.435
4.148ProLeu: 4.148 ± 0.551
0.302ProMet: 0.302 ± 0.151
1.584ProAsn: 1.584 ± 0.325
2.338ProPro: 2.338 ± 0.442
1.282ProGln: 1.282 ± 0.314
2.639ProArg: 2.639 ± 0.568
3.846ProSer: 3.846 ± 0.632
3.092ProThr: 3.092 ± 0.57
3.318ProVal: 3.318 ± 0.585
1.282ProTrp: 1.282 ± 0.326
0.679ProTyr: 0.679 ± 0.258
0.0ProXaa: 0.0 ± 0.0
Gln
4.902GlnAla: 4.902 ± 0.589
0.075GlnCys: 0.075 ± 0.068
1.734GlnAsp: 1.734 ± 0.341
2.111GlnGlu: 2.111 ± 0.387
1.056GlnPhe: 1.056 ± 0.282
2.489GlnGly: 2.489 ± 0.407
0.452GlnHis: 0.452 ± 0.182
2.036GlnIle: 2.036 ± 0.345
1.056GlnLys: 1.056 ± 0.254
4.826GlnLeu: 4.826 ± 0.447
0.528GlnMet: 0.528 ± 0.194
1.131GlnAsn: 1.131 ± 0.256
1.734GlnPro: 1.734 ± 0.39
0.603GlnGln: 0.603 ± 0.183
1.885GlnArg: 1.885 ± 0.346
1.508GlnSer: 1.508 ± 0.315
2.187GlnThr: 2.187 ± 0.445
1.734GlnVal: 1.734 ± 0.333
0.754GlnTrp: 0.754 ± 0.296
0.679GlnTyr: 0.679 ± 0.283
0.0GlnXaa: 0.0 ± 0.0
Arg
7.164ArgAla: 7.164 ± 0.871
0.377ArgCys: 0.377 ± 0.187
4.902ArgAsp: 4.902 ± 0.698
3.921ArgGlu: 3.921 ± 0.526
2.187ArgPhe: 2.187 ± 0.415
5.203ArgGly: 5.203 ± 0.649
1.584ArgHis: 1.584 ± 0.368
2.715ArgIle: 2.715 ± 0.41
2.338ArgLys: 2.338 ± 0.486
4.826ArgLeu: 4.826 ± 0.429
0.905ArgMet: 0.905 ± 0.24
1.81ArgAsn: 1.81 ± 0.414
3.092ArgPro: 3.092 ± 0.664
2.338ArgGln: 2.338 ± 0.382
5.957ArgArg: 5.957 ± 0.862
3.695ArgSer: 3.695 ± 0.525
3.77ArgThr: 3.77 ± 0.581
4.6ArgVal: 4.6 ± 0.7
1.584ArgTrp: 1.584 ± 0.343
1.433ArgTyr: 1.433 ± 0.335
0.0ArgXaa: 0.0 ± 0.0
Ser
5.731SerAla: 5.731 ± 0.811
0.0SerCys: 0.0 ± 0.0
4.148SerAsp: 4.148 ± 0.749
2.338SerGlu: 2.338 ± 0.347
2.187SerPhe: 2.187 ± 0.457
4.6SerGly: 4.6 ± 0.55
0.905SerHis: 0.905 ± 0.344
3.243SerIle: 3.243 ± 0.533
2.564SerLys: 2.564 ± 0.41
4.902SerLeu: 4.902 ± 0.588
1.131SerMet: 1.131 ± 0.329
1.734SerAsn: 1.734 ± 0.412
2.262SerPro: 2.262 ± 0.389
1.207SerGln: 1.207 ± 0.282
3.393SerArg: 3.393 ± 0.612
2.941SerSer: 2.941 ± 0.437
3.393SerThr: 3.393 ± 0.496
3.393SerVal: 3.393 ± 0.596
1.207SerTrp: 1.207 ± 0.273
0.83SerTyr: 0.83 ± 0.212
0.0SerXaa: 0.0 ± 0.0
Thr
8.295ThrAla: 8.295 ± 1.269
0.679ThrCys: 0.679 ± 0.276
4.298ThrAsp: 4.298 ± 0.659
3.469ThrGlu: 3.469 ± 0.546
2.715ThrPhe: 2.715 ± 0.434
5.279ThrGly: 5.279 ± 0.782
1.282ThrHis: 1.282 ± 0.332
3.393ThrIle: 3.393 ± 0.508
2.564ThrLys: 2.564 ± 0.528
5.505ThrLeu: 5.505 ± 0.671
1.131ThrMet: 1.131 ± 0.319
1.734ThrAsn: 1.734 ± 0.379
3.77ThrPro: 3.77 ± 0.511
1.207ThrGln: 1.207 ± 0.334
3.997ThrArg: 3.997 ± 0.545
4.374ThrSer: 4.374 ± 0.725
5.354ThrThr: 5.354 ± 1.002
4.751ThrVal: 4.751 ± 0.612
1.508ThrTrp: 1.508 ± 0.31
1.508ThrTyr: 1.508 ± 0.368
0.0ThrXaa: 0.0 ± 0.0
Val
8.747ValAla: 8.747 ± 0.851
0.302ValCys: 0.302 ± 0.141
4.6ValAsp: 4.6 ± 0.672
3.243ValGlu: 3.243 ± 0.432
2.413ValPhe: 2.413 ± 0.51
5.128ValGly: 5.128 ± 0.59
1.282ValHis: 1.282 ± 0.293
2.941ValIle: 2.941 ± 0.423
2.413ValLys: 2.413 ± 0.382
5.957ValLeu: 5.957 ± 0.727
1.508ValMet: 1.508 ± 0.339
2.111ValAsn: 2.111 ± 0.453
3.846ValPro: 3.846 ± 0.546
2.413ValGln: 2.413 ± 0.38
4.751ValArg: 4.751 ± 0.771
3.243ValSer: 3.243 ± 0.533
5.656ValThr: 5.656 ± 0.764
5.656ValVal: 5.656 ± 0.605
1.584ValTrp: 1.584 ± 0.463
2.187ValTyr: 2.187 ± 0.419
0.0ValXaa: 0.0 ± 0.0
Trp
2.111TrpAla: 2.111 ± 0.405
0.075TrpCys: 0.075 ± 0.071
0.679TrpAsp: 0.679 ± 0.217
1.282TrpGlu: 1.282 ± 0.304
0.754TrpPhe: 0.754 ± 0.229
1.508TrpGly: 1.508 ± 0.322
0.377TrpHis: 0.377 ± 0.207
1.131TrpIle: 1.131 ± 0.302
0.83TrpLys: 0.83 ± 0.227
2.036TrpLeu: 2.036 ± 0.338
0.83TrpMet: 0.83 ± 0.247
1.131TrpAsn: 1.131 ± 0.323
0.754TrpPro: 0.754 ± 0.246
1.131TrpGln: 1.131 ± 0.364
0.754TrpArg: 0.754 ± 0.274
1.734TrpSer: 1.734 ± 0.38
1.282TrpThr: 1.282 ± 0.289
2.187TrpVal: 2.187 ± 0.383
0.226TrpTrp: 0.226 ± 0.128
0.528TrpTyr: 0.528 ± 0.23
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.262TyrAla: 2.262 ± 0.365
0.377TyrCys: 0.377 ± 0.223
1.961TyrAsp: 1.961 ± 0.403
1.734TyrGlu: 1.734 ± 0.388
0.528TyrPhe: 0.528 ± 0.17
2.187TyrGly: 2.187 ± 0.463
0.528TyrHis: 0.528 ± 0.179
0.98TyrIle: 0.98 ± 0.262
0.377TyrLys: 0.377 ± 0.193
1.508TyrLeu: 1.508 ± 0.274
0.528TyrMet: 0.528 ± 0.19
0.905TyrAsn: 0.905 ± 0.273
1.357TyrPro: 1.357 ± 0.281
0.83TyrGln: 0.83 ± 0.288
2.338TyrArg: 2.338 ± 0.509
1.508TyrSer: 1.508 ± 0.348
2.036TyrThr: 2.036 ± 0.317
1.734TyrVal: 1.734 ± 0.43
0.603TyrTrp: 0.603 ± 0.213
0.452TyrTyr: 0.452 ± 0.195
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 69 proteins (13262 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski