Capybara microvirus Cap3_SP_470
Average proteome isoelectric point is 4.82
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P8W5A5|A0A4P8W5A5_9VIRU Minor capsid protein OS=Capybara microvirus Cap3_SP_470 OX=2585465 PE=4 SV=1
MM1 pKa = 7.79 IYY3 pKa = 10.49 GIYY6 pKa = 10.07 CMKK9 pKa = 10.48 DD10 pKa = 3.09 VKK12 pKa = 10.24 TGFLNPFVEE21 pKa = 4.49 VNDD24 pKa = 3.97 DD25 pKa = 3.47 VAVRR29 pKa = 11.84 GFANAMMAPGTVYY42 pKa = 11.17 SNFSADD48 pKa = 3.26 FSLYY52 pKa = 10.43 KK53 pKa = 10.62 VGLFDD58 pKa = 5.96 SEE60 pKa = 4.99 LCVLSGVDD68 pKa = 3.12 KK69 pKa = 11.35 VLIAEE74 pKa = 4.9 ASQFVKK80 pKa = 10.89
Molecular weight: 8.76 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.38
IPC2_protein 4.596
IPC_protein 4.38
Toseland 4.19
ProMoST 4.533
Dawson 4.368
Bjellqvist 4.52
Wikipedia 4.304
Rodwell 4.215
Grimsley 4.101
Solomon 4.355
Lehninger 4.304
Nozaki 4.482
DTASelect 4.711
Thurlkill 4.24
EMBOSS 4.304
Sillero 4.495
Patrickios 3.249
IPC_peptide 4.342
IPC2_peptide 4.482
IPC2.peptide.svr19 4.449
Protein with the highest isoelectric point:
>tr|A0A4V1FVU1|A0A4V1FVU1_9VIRU Replication initiator protein OS=Capybara microvirus Cap3_SP_470 OX=2585465 PE=4 SV=1
MM1 pKa = 7.49 NSRR4 pKa = 11.84 FAVAPQVNINRR15 pKa = 11.84 STFRR19 pKa = 11.84 RR20 pKa = 11.84 DD21 pKa = 3.17 HH22 pKa = 5.11 TVKK25 pKa = 10.05 TSFNMGEE32 pKa = 4.66 LIPFCVEE39 pKa = 3.48 EE40 pKa = 4.47 VLPGDD45 pKa = 3.63 TFSIKK50 pKa = 8.91 TSKK53 pKa = 10.8 LIRR56 pKa = 11.84 TQPLLTPLMDD66 pKa = 3.69 QLYY69 pKa = 10.75 LDD71 pKa = 4.24 TYY73 pKa = 10.35 WYY75 pKa = 9.69 FVPNRR80 pKa = 11.84 LIWEE84 pKa = 3.82 HH85 pKa = 6.93 WINFMGEE92 pKa = 4.08 NTDD95 pKa = 3.7 GSWIPEE101 pKa = 4.08 TQYY104 pKa = 11.57 SVPTVSAPSSTGWIEE119 pKa = 3.9 HH120 pKa = 5.93 TLADD124 pKa = 4.48 YY125 pKa = 9.9 MGLPVNVPSSSDD137 pKa = 3.31 GVFSVSALPFRR148 pKa = 11.84 AYY150 pKa = 10.59 CKK152 pKa = 10.05 IYY154 pKa = 10.65 NDD156 pKa = 3.35 WFRR159 pKa = 11.84 DD160 pKa = 3.61 EE161 pKa = 4.93 NLCDD165 pKa = 3.72 PAFLPLDD172 pKa = 3.89 DD173 pKa = 4.02 STRR176 pKa = 11.84 VGSNGDD182 pKa = 3.7 SLNDD186 pKa = 3.31 VCLGGMPLKK195 pKa = 10.48 SGKK198 pKa = 9.12 IHH200 pKa = 7.77 DD201 pKa = 4.59 YY202 pKa = 10.78 FSSCLPAPQKK212 pKa = 10.77 GSPVSLGLEE221 pKa = 4.04 SVYY224 pKa = 11.16 GDD226 pKa = 3.06 GFVPVFGNGLSLGLHH241 pKa = 5.12 NSEE244 pKa = 4.39 RR245 pKa = 11.84 LFGISSSNSSGLLGASSNAVGNPLGTTTSSIVPIQNKK282 pKa = 10.25 SIGVLTKK289 pKa = 10.59 SGLDD293 pKa = 3.54 AVGLPYY299 pKa = 10.51 KK300 pKa = 10.1 DD301 pKa = 3.41 TGLVADD307 pKa = 4.81 VSQSSLTVNSLRR319 pKa = 11.84 YY320 pKa = 9.36 AFAVQRR326 pKa = 11.84 LLEE329 pKa = 4.37 KK330 pKa = 10.4 DD331 pKa = 3.05 ARR333 pKa = 11.84 GGSRR337 pKa = 11.84 YY338 pKa = 9.2 IEE340 pKa = 3.95 QIKK343 pKa = 9.88 SHH345 pKa = 6.73 FGVTSPDD352 pKa = 2.98 ARR354 pKa = 11.84 LQRR357 pKa = 11.84 SEE359 pKa = 4.06 YY360 pKa = 10.84 LGGNRR365 pKa = 11.84 INININQVLNVAQAQKK381 pKa = 9.02 TGSSEE386 pKa = 4.0 GMLGNVAGYY395 pKa = 10.35 SVTGDD400 pKa = 3.3 SHH402 pKa = 8.24 GDD404 pKa = 3.34 FSKK407 pKa = 11.1 SFTEE411 pKa = 3.58 HH412 pKa = 6.5 GYY414 pKa = 10.25 IIGLMTVRR422 pKa = 11.84 QDD424 pKa = 3.12 NSYY427 pKa = 11.04 CQGTDD432 pKa = 3.19 RR433 pKa = 11.84 FWKK436 pKa = 9.77 RR437 pKa = 11.84 KK438 pKa = 6.8 TRR440 pKa = 11.84 FDD442 pKa = 3.24 YY443 pKa = 10.8 YY444 pKa = 11.31 YY445 pKa = 10.13 PVFANIGEE453 pKa = 4.24 QPVYY457 pKa = 10.31 QSEE460 pKa = 4.41 IYY462 pKa = 8.31 EE463 pKa = 4.3 TGSNSDD469 pKa = 2.83 IVFGYY474 pKa = 9.6 QEE476 pKa = 3.41 AWADD480 pKa = 3.69 YY481 pKa = 9.96 RR482 pKa = 11.84 FKK484 pKa = 10.56 PSKK487 pKa = 8.03 VTGMMRR493 pKa = 11.84 NQLDD497 pKa = 3.26 AWHH500 pKa = 6.86 LADD503 pKa = 5.24 KK504 pKa = 10.84 YY505 pKa = 11.33 NNPPVLGKK513 pKa = 8.1 TWIEE517 pKa = 3.7 SDD519 pKa = 3.46 KK520 pKa = 11.65 ANLDD524 pKa = 3.25 RR525 pKa = 11.84 ALAVTSSTADD535 pKa = 3.04 QFFADD540 pKa = 4.04 IFIEE544 pKa = 4.34 NKK546 pKa = 8.05 CTRR549 pKa = 11.84 PMPMFSIPGLLDD561 pKa = 3.34 HH562 pKa = 6.99 NN563 pKa = 4.67
Molecular weight: 62.23 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.377
IPC2_protein 5.41
IPC_protein 5.423
Toseland 5.664
ProMoST 5.69
Dawson 5.575
Bjellqvist 5.639
Wikipedia 5.55
Rodwell 5.525
Grimsley 5.741
Solomon 5.575
Lehninger 5.563
Nozaki 5.792
DTASelect 5.995
Thurlkill 5.855
EMBOSS 5.83
Sillero 5.881
Patrickios 3.834
IPC_peptide 5.588
IPC2_peptide 5.881
IPC2.peptide.svr19 5.867
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
1445
80
563
289.0
32.0
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.336 ± 1.792
1.038 ± 0.285
6.436 ± 1.046
5.19 ± 0.756
4.498 ± 0.684
6.92 ± 0.755
1.384 ± 0.414
5.19 ± 0.935
4.983 ± 0.599
8.235 ± 0.97
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.353 ± 0.273
6.505 ± 0.439
4.014 ± 0.717
3.875 ± 0.566
3.668 ± 0.534
11.003 ± 1.821
5.26 ± 0.606
6.09 ± 0.754
1.522 ± 0.167
4.498 ± 0.529
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here