Nocardia sp. CT2-14

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Corynebacteriales; Nocardiaceae; Nocardia; unclassified Nocardia

Average proteome isoelectric point is 6.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6379 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6I3L3G8|A0A6I3L3G8_9NOCA Uncharacterized protein OS=Nocardia sp. CT2-14 OX=2675850 GN=GLP40_20700 PE=4 SV=1
MM1 pKa = 7.69NDD3 pKa = 3.08YY4 pKa = 10.54KK5 pKa = 11.41VYY7 pKa = 11.05VCTQCGFEE15 pKa = 3.98YY16 pKa = 10.77DD17 pKa = 3.85EE18 pKa = 4.6AKK20 pKa = 10.37GWPEE24 pKa = 3.38EE25 pKa = 4.84DD26 pKa = 3.12IAPGTRR32 pKa = 11.84WADD35 pKa = 3.36IPDD38 pKa = 3.99DD39 pKa = 4.34WSCPDD44 pKa = 3.97CGAAKK49 pKa = 10.45ADD51 pKa = 3.42FDD53 pKa = 3.94MVEE56 pKa = 4.04VEE58 pKa = 4.32RR59 pKa = 11.84AA60 pKa = 3.09

Molecular weight: 6.81 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6I3KU43|A0A6I3KU43_9NOCA Translation initiation factor IF-1 OS=Nocardia sp. CT2-14 OX=2675850 GN=infA PE=3 SV=1
MM1 pKa = 7.69AKK3 pKa = 10.06GKK5 pKa = 8.69RR6 pKa = 11.84TFQPNNRR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84ARR17 pKa = 11.84VHH19 pKa = 5.99GFRR22 pKa = 11.84LRR24 pKa = 11.84MRR26 pKa = 11.84TRR28 pKa = 11.84AGRR31 pKa = 11.84AIVSARR37 pKa = 11.84RR38 pKa = 11.84GKK40 pKa = 10.41GRR42 pKa = 11.84AKK44 pKa = 9.65LTAA47 pKa = 4.21

Molecular weight: 5.42 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6379

0

6379

2074707

16

14878

325.2

34.94

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.298 ± 0.045

0.744 ± 0.01

6.118 ± 0.03

5.456 ± 0.027

2.975 ± 0.019

8.845 ± 0.029

2.281 ± 0.015

4.236 ± 0.02

2.059 ± 0.024

10.157 ± 0.036

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.95 ± 0.013

2.084 ± 0.016

5.889 ± 0.029

2.865 ± 0.016

7.709 ± 0.029

5.244 ± 0.022

6.138 ± 0.021

8.404 ± 0.033

1.463 ± 0.014

2.084 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski