Amino acid dipepetide frequency for Nocardia sp. CT2-14

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
21.296AlaAla: 21.296 ± 0.175
0.926AlaCys: 0.926 ± 0.025
8.619AlaAsp: 8.619 ± 0.083
8.565AlaGlu: 8.565 ± 0.082
3.433AlaPhe: 3.433 ± 0.042
11.875AlaGly: 11.875 ± 0.075
2.833AlaHis: 2.833 ± 0.035
5.138AlaIle: 5.138 ± 0.063
2.854AlaLys: 2.854 ± 0.045
13.626AlaLeu: 13.626 ± 0.096
2.752AlaMet: 2.752 ± 0.04
2.468AlaAsn: 2.468 ± 0.034
6.335AlaPro: 6.335 ± 0.071
3.949AlaGln: 3.949 ± 0.046
9.459AlaArg: 9.459 ± 0.075
5.429AlaSer: 5.429 ± 0.049
7.49AlaThr: 7.49 ± 0.074
11.796AlaVal: 11.796 ± 0.109
1.698AlaTrp: 1.698 ± 0.032
2.436AlaTyr: 2.436 ± 0.044
0.0AlaXaa: 0.0 ± 0.0
Cys
1.033CysAla: 1.033 ± 0.02
0.094CysCys: 0.094 ± 0.009
0.445CysAsp: 0.445 ± 0.016
0.389CysGlu: 0.389 ± 0.016
0.215CysPhe: 0.215 ± 0.01
0.89CysGly: 0.89 ± 0.023
0.169CysHis: 0.169 ± 0.01
0.21CysIle: 0.21 ± 0.011
0.114CysLys: 0.114 ± 0.007
0.656CysLeu: 0.656 ± 0.02
0.109CysMet: 0.109 ± 0.007
0.15CysAsn: 0.15 ± 0.01
0.469CysPro: 0.469 ± 0.016
0.169CysGln: 0.169 ± 0.009
0.528CysArg: 0.528 ± 0.018
0.457CysSer: 0.457 ± 0.017
0.471CysThr: 0.471 ± 0.014
0.587CysVal: 0.587 ± 0.018
0.119CysTrp: 0.119 ± 0.007
0.162CysTyr: 0.162 ± 0.01
0.0CysXaa: 0.0 ± 0.0
Asp
7.441AspAla: 7.441 ± 0.071
0.429AspCys: 0.429 ± 0.016
3.522AspAsp: 3.522 ± 0.047
3.576AspGlu: 3.576 ± 0.043
1.809AspPhe: 1.809 ± 0.033
5.422AspGly: 5.422 ± 0.056
1.583AspHis: 1.583 ± 0.029
2.633AspIle: 2.633 ± 0.035
1.263AspLys: 1.263 ± 0.031
6.531AspLeu: 6.531 ± 0.071
1.011AspMet: 1.011 ± 0.021
1.199AspAsn: 1.199 ± 0.027
4.616AspPro: 4.616 ± 0.064
1.821AspGln: 1.821 ± 0.034
5.114AspArg: 5.114 ± 0.056
3.105AspSer: 3.105 ± 0.038
3.315AspThr: 3.315 ± 0.045
4.405AspVal: 4.405 ± 0.054
0.988AspTrp: 0.988 ± 0.025
1.396AspTyr: 1.396 ± 0.025
0.0AspXaa: 0.0 ± 0.0
Glu
6.121GluAla: 6.121 ± 0.069
0.352GluCys: 0.352 ± 0.014
2.362GluAsp: 2.362 ± 0.032
2.479GluGlu: 2.479 ± 0.042
2.035GluPhe: 2.035 ± 0.035
3.325GluGly: 3.325 ± 0.043
1.633GluHis: 1.633 ± 0.037
2.843GluIle: 2.843 ± 0.037
1.32GluLys: 1.32 ± 0.029
7.107GluLeu: 7.107 ± 0.067
1.021GluMet: 1.021 ± 0.023
1.206GluAsn: 1.206 ± 0.026
3.187GluPro: 3.187 ± 0.056
2.322GluGln: 2.322 ± 0.034
4.935GluArg: 4.935 ± 0.059
2.967GluSer: 2.967 ± 0.038
2.914GluThr: 2.914 ± 0.038
4.384GluVal: 4.384 ± 0.056
0.796GluTrp: 0.796 ± 0.022
1.248GluTyr: 1.248 ± 0.023
0.0GluXaa: 0.0 ± 0.0
Phe
4.086PheAla: 4.086 ± 0.055
0.26PheCys: 0.26 ± 0.011
2.466PheAsp: 2.466 ± 0.042
1.671PheGlu: 1.671 ± 0.036
0.903PhePhe: 0.903 ± 0.028
3.502PheGly: 3.502 ± 0.047
0.653PheHis: 0.653 ± 0.02
0.935PheIle: 0.935 ± 0.022
0.459PheLys: 0.459 ± 0.014
2.611PheLeu: 2.611 ± 0.037
0.46PheMet: 0.46 ± 0.014
0.598PheAsn: 0.598 ± 0.019
1.426PhePro: 1.426 ± 0.027
0.748PheGln: 0.748 ± 0.022
1.849PheArg: 1.849 ± 0.028
1.553PheSer: 1.553 ± 0.026
2.111PheThr: 2.111 ± 0.033
2.421PheVal: 2.421 ± 0.04
0.404PheTrp: 0.404 ± 0.014
0.635PheTyr: 0.635 ± 0.019
0.0PheXaa: 0.0 ± 0.0
Gly
10.244GlyAla: 10.244 ± 0.079
0.791GlyCys: 0.791 ± 0.022
4.875GlyAsp: 4.875 ± 0.051
4.542GlyGlu: 4.542 ± 0.051
3.051GlyPhe: 3.051 ± 0.04
7.707GlyGly: 7.707 ± 0.093
2.142GlyHis: 2.142 ± 0.033
4.301GlyIle: 4.301 ± 0.052
2.379GlyLys: 2.379 ± 0.038
8.803GlyLeu: 8.803 ± 0.068
2.121GlyMet: 2.121 ± 0.034
1.927GlyAsn: 1.927 ± 0.038
4.587GlyPro: 4.587 ± 0.057
2.553GlyGln: 2.553 ± 0.043
6.631GlyArg: 6.631 ± 0.063
5.213GlySer: 5.213 ± 0.061
5.378GlyThr: 5.378 ± 0.055
7.3GlyVal: 7.3 ± 0.068
1.592GlyTrp: 1.592 ± 0.032
2.314GlyTyr: 2.314 ± 0.035
0.0GlyXaa: 0.0 ± 0.0
His
2.639HisAla: 2.639 ± 0.037
0.215HisCys: 0.215 ± 0.011
1.346HisAsp: 1.346 ± 0.029
1.161HisGlu: 1.161 ± 0.022
0.672HisPhe: 0.672 ± 0.019
2.246HisGly: 2.246 ± 0.034
0.706HisHis: 0.706 ± 0.019
0.881HisIle: 0.881 ± 0.02
0.319HisLys: 0.319 ± 0.013
2.253HisLeu: 2.253 ± 0.033
0.403HisMet: 0.403 ± 0.014
0.49HisAsn: 0.49 ± 0.017
1.845HisPro: 1.845 ± 0.026
0.596HisGln: 0.596 ± 0.019
2.067HisArg: 2.067 ± 0.036
1.111HisSer: 1.111 ± 0.026
1.349HisThr: 1.349 ± 0.031
1.584HisVal: 1.584 ± 0.029
0.377HisTrp: 0.377 ± 0.014
0.549HisTyr: 0.549 ± 0.016
0.0HisXaa: 0.0 ± 0.0
Ile
6.497IleAla: 6.497 ± 0.064
0.316IleCys: 0.316 ± 0.012
3.313IleAsp: 3.313 ± 0.047
2.715IleGlu: 2.715 ± 0.036
0.952IlePhe: 0.952 ± 0.026
4.618IleGly: 4.618 ± 0.048
0.799IleHis: 0.799 ± 0.019
1.393IleIle: 1.393 ± 0.028
0.761IleLys: 0.761 ± 0.023
3.294IleLeu: 3.294 ± 0.043
0.649IleMet: 0.649 ± 0.02
0.899IleAsn: 0.899 ± 0.021
2.512IlePro: 2.512 ± 0.036
0.899IleGln: 0.899 ± 0.023
2.82IleArg: 2.82 ± 0.036
2.229IleSer: 2.229 ± 0.034
2.872IleThr: 2.872 ± 0.035
3.627IleVal: 3.627 ± 0.051
0.46IleTrp: 0.46 ± 0.016
0.738IleTyr: 0.738 ± 0.019
0.0IleXaa: 0.0 ± 0.0
Lys
2.531LysAla: 2.531 ± 0.041
0.103LysCys: 0.103 ± 0.008
1.103LysAsp: 1.103 ± 0.027
0.898LysGlu: 0.898 ± 0.025
0.602LysPhe: 0.602 ± 0.02
1.496LysGly: 1.496 ± 0.032
0.459LysHis: 0.459 ± 0.016
0.992LysIle: 0.992 ± 0.024
0.61LysLys: 0.61 ± 0.021
2.18LysLeu: 2.18 ± 0.033
0.456LysMet: 0.456 ± 0.015
0.509LysAsn: 0.509 ± 0.017
1.39LysPro: 1.39 ± 0.029
0.75LysGln: 0.75 ± 0.022
1.422LysArg: 1.422 ± 0.034
1.239LysSer: 1.239 ± 0.026
1.312LysThr: 1.312 ± 0.028
1.791LysVal: 1.791 ± 0.035
0.28LysTrp: 0.28 ± 0.013
0.468LysTyr: 0.468 ± 0.016
0.0LysXaa: 0.0 ± 0.0
Leu
14.607LeuAla: 14.607 ± 0.087
0.77LeuCys: 0.77 ± 0.019
6.884LeuAsp: 6.884 ± 0.065
4.984LeuGlu: 4.984 ± 0.052
2.772LeuPhe: 2.772 ± 0.052
9.016LeuGly: 9.016 ± 0.082
2.131LeuHis: 2.131 ± 0.034
4.2LeuIle: 4.2 ± 0.053
1.531LeuLys: 1.531 ± 0.031
10.166LeuLeu: 10.166 ± 0.09
1.668LeuMet: 1.668 ± 0.032
1.936LeuAsn: 1.936 ± 0.039
6.229LeuPro: 6.229 ± 0.062
2.264LeuGln: 2.264 ± 0.034
8.561LeuArg: 8.561 ± 0.082
5.762LeuSer: 5.762 ± 0.064
6.745LeuThr: 6.745 ± 0.062
8.436LeuVal: 8.436 ± 0.089
1.198LeuTrp: 1.198 ± 0.022
1.71LeuTyr: 1.71 ± 0.031
0.0LeuXaa: 0.0 ± 0.0
Met
2.451MetAla: 2.451 ± 0.034
0.144MetCys: 0.144 ± 0.009
0.932MetAsp: 0.932 ± 0.022
0.7MetGlu: 0.7 ± 0.018
0.601MetPhe: 0.601 ± 0.017
1.395MetGly: 1.395 ± 0.025
0.405MetHis: 0.405 ± 0.014
0.86MetIle: 0.86 ± 0.023
0.431MetLys: 0.431 ± 0.015
2.052MetLeu: 2.052 ± 0.031
0.368MetMet: 0.368 ± 0.014
0.495MetAsn: 0.495 ± 0.017
1.267MetPro: 1.267 ± 0.026
0.494MetGln: 0.494 ± 0.015
1.565MetArg: 1.565 ± 0.03
1.475MetSer: 1.475 ± 0.029
1.66MetThr: 1.66 ± 0.026
1.606MetVal: 1.606 ± 0.033
0.244MetTrp: 0.244 ± 0.012
0.358MetTyr: 0.358 ± 0.016
0.0MetXaa: 0.0 ± 0.0
Asn
2.545AsnAla: 2.545 ± 0.04
0.167AsnCys: 0.167 ± 0.009
1.056AsnAsp: 1.056 ± 0.027
0.885AsnGlu: 0.885 ± 0.02
0.597AsnPhe: 0.597 ± 0.018
2.05AsnGly: 2.05 ± 0.041
0.461AsnHis: 0.461 ± 0.016
0.893AsnIle: 0.893 ± 0.024
0.443AsnLys: 0.443 ± 0.017
2.071AsnLeu: 2.071 ± 0.033
0.417AsnMet: 0.417 ± 0.013
0.483AsnAsn: 0.483 ± 0.017
1.794AsnPro: 1.794 ± 0.031
0.62AsnGln: 0.62 ± 0.018
1.554AsnArg: 1.554 ± 0.027
1.169AsnSer: 1.169 ± 0.024
1.272AsnThr: 1.272 ± 0.028
1.523AsnVal: 1.523 ± 0.028
0.345AsnTrp: 0.345 ± 0.013
0.499AsnTyr: 0.499 ± 0.016
0.0AsnXaa: 0.0 ± 0.0
Pro
7.938ProAla: 7.938 ± 0.081
0.302ProCys: 0.302 ± 0.012
4.467ProAsp: 4.467 ± 0.046
4.143ProGlu: 4.143 ± 0.055
1.573ProPhe: 1.573 ± 0.03
5.942ProGly: 5.942 ± 0.074
1.287ProHis: 1.287 ± 0.028
2.309ProIle: 2.309 ± 0.032
1.286ProLys: 1.286 ± 0.029
5.05ProLeu: 5.05 ± 0.049
1.116ProMet: 1.116 ± 0.022
1.285ProAsn: 1.285 ± 0.03
3.089ProPro: 3.089 ± 0.057
1.825ProGln: 1.825 ± 0.041
3.696ProArg: 3.696 ± 0.055
2.957ProSer: 2.957 ± 0.048
3.408ProThr: 3.408 ± 0.045
5.294ProVal: 5.294 ± 0.067
0.823ProTrp: 0.823 ± 0.021
1.099ProTyr: 1.099 ± 0.028
0.0ProXaa: 0.0 ± 0.0
Gln
3.491GlnAla: 3.491 ± 0.042
0.189GlnCys: 0.189 ± 0.009
1.188GlnAsp: 1.188 ± 0.025
1.109GlnGlu: 1.109 ± 0.029
0.888GlnPhe: 0.888 ± 0.02
1.969GlnGly: 1.969 ± 0.039
0.692GlnHis: 0.692 ± 0.019
1.368GlnIle: 1.368 ± 0.027
0.543GlnLys: 0.543 ± 0.017
3.518GlnLeu: 3.518 ± 0.043
0.581GlnMet: 0.581 ± 0.018
0.548GlnAsn: 0.548 ± 0.017
1.922GlnPro: 1.922 ± 0.041
1.326GlnGln: 1.326 ± 0.035
2.803GlnArg: 2.803 ± 0.038
1.369GlnSer: 1.369 ± 0.03
1.373GlnThr: 1.373 ± 0.026
2.618GlnVal: 2.618 ± 0.045
0.526GlnTrp: 0.526 ± 0.018
0.634GlnTyr: 0.634 ± 0.017
0.0GlnXaa: 0.0 ± 0.0
Arg
9.291ArgAla: 9.291 ± 0.083
0.545ArgCys: 0.545 ± 0.018
4.347ArgAsp: 4.347 ± 0.056
4.38ArgGlu: 4.38 ± 0.051
2.545ArgPhe: 2.545 ± 0.038
5.404ArgGly: 5.404 ± 0.048
1.91ArgHis: 1.91 ± 0.033
3.822ArgIle: 3.822 ± 0.043
1.715ArgLys: 1.715 ± 0.032
7.952ArgLeu: 7.952 ± 0.072
1.968ArgMet: 1.968 ± 0.028
1.645ArgAsn: 1.645 ± 0.03
4.357ArgPro: 4.357 ± 0.055
2.25ArgGln: 2.25 ± 0.035
6.851ArgArg: 6.851 ± 0.076
3.989ArgSer: 3.989 ± 0.043
4.962ArgThr: 4.962 ± 0.048
5.885ArgVal: 5.885 ± 0.056
1.371ArgTrp: 1.371 ± 0.028
1.9ArgTyr: 1.9 ± 0.033
0.0ArgXaa: 0.0 ± 0.0
Ser
7.017SerAla: 7.017 ± 0.059
0.393SerCys: 0.393 ± 0.015
3.142SerAsp: 3.142 ± 0.041
2.67SerGlu: 2.67 ± 0.039
1.605SerPhe: 1.605 ± 0.027
5.854SerGly: 5.854 ± 0.064
1.031SerHis: 1.031 ± 0.026
2.129SerIle: 2.129 ± 0.033
1.18SerLys: 1.18 ± 0.028
4.615SerLeu: 4.615 ± 0.049
1.292SerMet: 1.292 ± 0.027
1.118SerAsn: 1.118 ± 0.024
2.948SerPro: 2.948 ± 0.043
1.331SerGln: 1.331 ± 0.029
3.636SerArg: 3.636 ± 0.04
2.903SerSer: 2.903 ± 0.042
3.248SerThr: 3.248 ± 0.04
4.314SerVal: 4.314 ± 0.046
0.873SerTrp: 0.873 ± 0.021
1.145SerTyr: 1.145 ± 0.028
0.0SerXaa: 0.0 ± 0.0
Thr
8.631ThrAla: 8.631 ± 0.08
0.403ThrCys: 0.403 ± 0.015
3.756ThrAsp: 3.756 ± 0.045
3.391ThrGlu: 3.391 ± 0.044
1.719ThrPhe: 1.719 ± 0.031
6.2ThrGly: 6.2 ± 0.057
1.198ThrHis: 1.198 ± 0.024
2.401ThrIle: 2.401 ± 0.037
1.169ThrLys: 1.169 ± 0.026
5.89ThrLeu: 5.89 ± 0.057
1.055ThrMet: 1.055 ± 0.025
1.125ThrAsn: 1.125 ± 0.024
4.048ThrPro: 4.048 ± 0.052
1.425ThrGln: 1.425 ± 0.029
3.944ThrArg: 3.944 ± 0.048
2.983ThrSer: 2.983 ± 0.039
3.925ThrThr: 3.925 ± 0.054
6.125ThrVal: 6.125 ± 0.051
0.791ThrTrp: 0.791 ± 0.017
1.202ThrTyr: 1.202 ± 0.028
0.0ThrXaa: 0.0 ± 0.0
Val
11.151ValAla: 11.151 ± 0.1
0.697ValCys: 0.697 ± 0.019
5.286ValAsp: 5.286 ± 0.049
4.557ValGlu: 4.557 ± 0.052
2.547ValPhe: 2.547 ± 0.041
6.547ValGly: 6.547 ± 0.066
1.899ValHis: 1.899 ± 0.027
3.708ValIle: 3.708 ± 0.044
1.528ValLys: 1.528 ± 0.034
9.183ValLeu: 9.183 ± 0.087
1.434ValMet: 1.434 ± 0.028
1.888ValAsn: 1.888 ± 0.032
4.891ValPro: 4.891 ± 0.058
2.056ValGln: 2.056 ± 0.032
6.41ValArg: 6.41 ± 0.055
4.496ValSer: 4.496 ± 0.046
5.448ValThr: 5.448 ± 0.053
7.722ValVal: 7.722 ± 0.073
1.043ValTrp: 1.043 ± 0.024
1.544ValTyr: 1.544 ± 0.03
0.0ValXaa: 0.0 ± 0.0
Trp
1.501TrpAla: 1.501 ± 0.026
0.142TrpCys: 0.142 ± 0.008
0.757TrpAsp: 0.757 ± 0.02
0.699TrpGlu: 0.699 ± 0.018
0.525TrpPhe: 0.525 ± 0.017
0.95TrpGly: 0.95 ± 0.022
0.367TrpHis: 0.367 ± 0.015
0.669TrpIle: 0.669 ± 0.019
0.341TrpLys: 0.341 ± 0.012
1.676TrpLeu: 1.676 ± 0.029
0.326TrpMet: 0.326 ± 0.013
0.444TrpAsn: 0.444 ± 0.016
0.746TrpPro: 0.746 ± 0.022
0.603TrpGln: 0.603 ± 0.019
1.294TrpArg: 1.294 ± 0.028
0.922TrpSer: 0.922 ± 0.02
0.967TrpThr: 0.967 ± 0.027
1.04TrpVal: 1.04 ± 0.027
0.339TrpTrp: 0.339 ± 0.015
0.325TrpTyr: 0.325 ± 0.014
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.464TyrAla: 2.464 ± 0.035
0.201TyrCys: 0.201 ± 0.011
1.313TyrAsp: 1.313 ± 0.026
1.042TyrGlu: 1.042 ± 0.023
0.708TyrPhe: 0.708 ± 0.019
2.044TyrGly: 2.044 ± 0.035
0.449TyrHis: 0.449 ± 0.015
0.677TyrIle: 0.677 ± 0.018
0.346TyrLys: 0.346 ± 0.015
2.334TyrLeu: 2.334 ± 0.039
0.294TyrMet: 0.294 ± 0.012
0.433TyrAsn: 0.433 ± 0.012
1.231TyrPro: 1.231 ± 0.024
0.654TyrGln: 0.654 ± 0.017
1.949TyrArg: 1.949 ± 0.03
1.118TyrSer: 1.118 ± 0.024
1.162TyrThr: 1.162 ± 0.025
1.578TyrVal: 1.578 ± 0.037
0.365TyrTrp: 0.365 ± 0.013
0.481TyrTyr: 0.481 ± 0.015
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 6379 proteins (2074707 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski