Staphylococcus phage ROSA

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Azeredovirinae; Phietavirus; Staphylococcus virus ROSA

Average proteome isoelectric point is 6.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 74 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q4ZBL3|Q4ZBL3_9CAUD ORF030 OS=Staphylococcus phage ROSA OX=320843 PE=4 SV=1
MM1 pKa = 6.89QQQAYY6 pKa = 9.38INATIDD12 pKa = 2.95IRR14 pKa = 11.84IPTEE18 pKa = 3.49VEE20 pKa = 3.58YY21 pKa = 10.91QYY23 pKa = 11.49FDD25 pKa = 3.82DD26 pKa = 5.0VDD28 pKa = 3.64IEE30 pKa = 4.33KK31 pKa = 10.59EE32 pKa = 3.88ALADD36 pKa = 3.61YY37 pKa = 10.58LYY39 pKa = 11.17NNPDD43 pKa = 3.26EE44 pKa = 4.53LLEE47 pKa = 3.95YY48 pKa = 11.05DD49 pKa = 3.49NLKK52 pKa = 9.69IRR54 pKa = 11.84NVNVEE59 pKa = 3.93VEE61 pKa = 4.1

Molecular weight: 7.3 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q4ZBM9|Q4ZBM9_9CAUD ORF115 OS=Staphylococcus phage ROSA OX=320843 PE=4 SV=1
MM1 pKa = 7.2NVAILANPAIDD12 pKa = 3.52HH13 pKa = 6.54AVVKK17 pKa = 9.77YY18 pKa = 10.59RR19 pKa = 11.84NGISTIAKK27 pKa = 6.5TVKK30 pKa = 10.37HH31 pKa = 5.12NTATRR36 pKa = 3.41

Molecular weight: 3.89 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

74

0

74

13540

35

1047

183.0

20.94

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.753 ± 0.378

0.51 ± 0.099

6.137 ± 0.255

7.474 ± 0.49

4.247 ± 0.228

5.908 ± 0.335

1.876 ± 0.135

7.629 ± 0.277

9.365 ± 0.394

7.496 ± 0.266

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.644 ± 0.192

6.558 ± 0.289

2.806 ± 0.185

3.988 ± 0.223

4.04 ± 0.243

5.82 ± 0.308

6.078 ± 0.249

6.123 ± 0.311

1.211 ± 0.153

4.335 ± 0.357
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski