Amino acid dipepetide frequency for Staphylococcus phage ROSA

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
0.665AlaAla: 0.665 ± 0.182
0.295AlaCys: 0.295 ± 0.14
2.216AlaAsp: 2.216 ± 0.376
4.949AlaGlu: 4.949 ± 0.682
2.733AlaPhe: 2.733 ± 0.58
3.693AlaGly: 3.693 ± 0.664
1.256AlaHis: 1.256 ± 0.282
5.17AlaIle: 5.17 ± 0.516
6.057AlaLys: 6.057 ± 0.746
4.506AlaLeu: 4.506 ± 0.715
1.551AlaMet: 1.551 ± 0.339
3.619AlaAsn: 3.619 ± 0.526
1.477AlaPro: 1.477 ± 0.346
2.29AlaGln: 2.29 ± 0.353
2.364AlaArg: 2.364 ± 0.381
3.102AlaSer: 3.102 ± 0.542
3.767AlaThr: 3.767 ± 0.439
4.579AlaVal: 4.579 ± 0.72
0.96AlaTrp: 0.96 ± 0.369
2.29AlaTyr: 2.29 ± 0.371
0.0AlaXaa: 0.0 ± 0.0
Cys
0.074CysAla: 0.074 ± 0.073
0.074CysCys: 0.074 ± 0.074
0.148CysAsp: 0.148 ± 0.111
0.517CysGlu: 0.517 ± 0.29
0.148CysPhe: 0.148 ± 0.144
0.517CysGly: 0.517 ± 0.217
0.148CysHis: 0.148 ± 0.115
0.295CysIle: 0.295 ± 0.137
0.369CysLys: 0.369 ± 0.146
0.295CysLeu: 0.295 ± 0.174
0.074CysMet: 0.074 ± 0.081
0.295CysAsn: 0.295 ± 0.147
0.148CysPro: 0.148 ± 0.096
0.222CysGln: 0.222 ± 0.119
0.369CysArg: 0.369 ± 0.215
0.443CysSer: 0.443 ± 0.19
0.074CysThr: 0.074 ± 0.087
0.222CysVal: 0.222 ± 0.137
0.148CysTrp: 0.148 ± 0.099
0.517CysTyr: 0.517 ± 0.178
0.0CysXaa: 0.0 ± 0.0
Asp
3.398AspAla: 3.398 ± 0.578
0.443AspCys: 0.443 ± 0.189
4.358AspAsp: 4.358 ± 0.624
5.023AspGlu: 5.023 ± 0.689
3.176AspPhe: 3.176 ± 0.594
4.284AspGly: 4.284 ± 0.508
0.295AspHis: 0.295 ± 0.158
5.318AspIle: 5.318 ± 0.497
6.352AspLys: 6.352 ± 0.873
5.244AspLeu: 5.244 ± 0.482
2.437AspMet: 2.437 ± 0.437
3.841AspAsn: 3.841 ± 0.623
1.329AspPro: 1.329 ± 0.265
0.591AspGln: 0.591 ± 0.19
2.068AspArg: 2.068 ± 0.398
3.25AspSer: 3.25 ± 0.528
3.176AspThr: 3.176 ± 0.439
3.471AspVal: 3.471 ± 0.581
0.812AspTrp: 0.812 ± 0.229
2.437AspTyr: 2.437 ± 0.388
0.0AspXaa: 0.0 ± 0.0
Glu
4.432GluAla: 4.432 ± 0.593
0.443GluCys: 0.443 ± 0.203
3.693GluAsp: 3.693 ± 0.633
4.875GluGlu: 4.875 ± 0.824
3.028GluPhe: 3.028 ± 0.47
3.545GluGly: 3.545 ± 0.57
1.699GluHis: 1.699 ± 0.328
5.17GluIle: 5.17 ± 0.636
6.574GluLys: 6.574 ± 0.987
7.46GluLeu: 7.46 ± 0.95
2.216GluMet: 2.216 ± 0.406
4.727GluAsn: 4.727 ± 0.623
1.847GluPro: 1.847 ± 0.361
4.284GluGln: 4.284 ± 0.653
3.25GluArg: 3.25 ± 0.42
4.062GluSer: 4.062 ± 0.615
3.398GluThr: 3.398 ± 0.523
5.096GluVal: 5.096 ± 0.533
1.034GluTrp: 1.034 ± 0.294
3.915GluTyr: 3.915 ± 0.643
0.0GluXaa: 0.0 ± 0.0
Phe
2.29PheAla: 2.29 ± 0.352
0.222PheCys: 0.222 ± 0.13
3.324PheAsp: 3.324 ± 0.372
3.545PheGlu: 3.545 ± 0.611
0.96PhePhe: 0.96 ± 0.239
2.733PheGly: 2.733 ± 0.58
1.034PheHis: 1.034 ± 0.283
3.398PheIle: 3.398 ± 0.443
3.767PheLys: 3.767 ± 0.584
2.068PheLeu: 2.068 ± 0.29
1.034PheMet: 1.034 ± 0.27
3.693PheAsn: 3.693 ± 0.461
0.886PhePro: 0.886 ± 0.298
1.034PheGln: 1.034 ± 0.32
1.403PheArg: 1.403 ± 0.264
3.028PheSer: 3.028 ± 0.527
2.954PheThr: 2.954 ± 0.525
2.954PheVal: 2.954 ± 0.495
0.222PheTrp: 0.222 ± 0.122
1.92PheTyr: 1.92 ± 0.364
0.0PheXaa: 0.0 ± 0.0
Gly
4.062GlyAla: 4.062 ± 0.543
0.369GlyCys: 0.369 ± 0.157
3.471GlyAsp: 3.471 ± 0.574
3.915GlyGlu: 3.915 ± 0.601
2.954GlyPhe: 2.954 ± 0.471
2.733GlyGly: 2.733 ± 0.508
1.994GlyHis: 1.994 ± 0.414
4.506GlyIle: 4.506 ± 0.585
4.949GlyLys: 4.949 ± 0.574
4.432GlyLeu: 4.432 ± 0.588
1.551GlyMet: 1.551 ± 0.299
3.25GlyAsn: 3.25 ± 0.529
0.517GlyPro: 0.517 ± 0.212
3.028GlyGln: 3.028 ± 0.436
2.068GlyArg: 2.068 ± 0.44
3.176GlySer: 3.176 ± 0.375
3.398GlyThr: 3.398 ± 0.594
4.875GlyVal: 4.875 ± 0.707
0.739GlyTrp: 0.739 ± 0.261
3.102GlyTyr: 3.102 ± 0.56
0.0GlyXaa: 0.0 ± 0.0
His
1.329HisAla: 1.329 ± 0.312
0.074HisCys: 0.074 ± 0.082
0.812HisAsp: 0.812 ± 0.215
0.96HisGlu: 0.96 ± 0.265
0.886HisPhe: 0.886 ± 0.227
1.256HisGly: 1.256 ± 0.309
0.591HisHis: 0.591 ± 0.251
1.256HisIle: 1.256 ± 0.321
1.403HisLys: 1.403 ± 0.3
1.699HisLeu: 1.699 ± 0.323
0.443HisMet: 0.443 ± 0.156
0.886HisAsn: 0.886 ± 0.24
0.886HisPro: 0.886 ± 0.272
1.034HisGln: 1.034 ± 0.28
0.739HisArg: 0.739 ± 0.308
1.182HisSer: 1.182 ± 0.352
1.034HisThr: 1.034 ± 0.314
1.256HisVal: 1.256 ± 0.321
0.074HisTrp: 0.074 ± 0.077
0.96HisTyr: 0.96 ± 0.355
0.0HisXaa: 0.0 ± 0.0
Ile
4.653IleAla: 4.653 ± 0.567
0.148IleCys: 0.148 ± 0.103
5.392IleAsp: 5.392 ± 0.654
6.943IleGlu: 6.943 ± 0.954
2.807IlePhe: 2.807 ± 0.546
4.579IleGly: 4.579 ± 0.887
1.256IleHis: 1.256 ± 0.325
3.767IleIle: 3.767 ± 0.541
7.608IleLys: 7.608 ± 0.783
4.875IleLeu: 4.875 ± 0.559
2.585IleMet: 2.585 ± 0.452
5.392IleAsn: 5.392 ± 0.787
2.216IlePro: 2.216 ± 0.348
2.733IleGln: 2.733 ± 0.351
3.767IleArg: 3.767 ± 0.517
4.432IleSer: 4.432 ± 0.641
5.318IleThr: 5.318 ± 0.607
3.988IleVal: 3.988 ± 0.47
1.108IleTrp: 1.108 ± 0.327
2.733IleTyr: 2.733 ± 0.5
0.0IleXaa: 0.0 ± 0.0
Lys
6.574LysAla: 6.574 ± 0.697
0.443LysCys: 0.443 ± 0.21
4.949LysAsp: 4.949 ± 0.735
7.091LysGlu: 7.091 ± 0.688
4.358LysPhe: 4.358 ± 0.607
5.761LysGly: 5.761 ± 0.75
1.994LysHis: 1.994 ± 0.435
7.386LysIle: 7.386 ± 0.954
8.346LysLys: 8.346 ± 1.084
6.943LysLeu: 6.943 ± 0.739
2.29LysMet: 2.29 ± 0.363
5.096LysAsn: 5.096 ± 0.789
2.807LysPro: 2.807 ± 0.486
4.21LysGln: 4.21 ± 0.518
4.432LysArg: 4.432 ± 0.604
4.801LysSer: 4.801 ± 0.554
5.761LysThr: 5.761 ± 0.685
5.096LysVal: 5.096 ± 0.442
1.108LysTrp: 1.108 ± 0.27
4.21LysTyr: 4.21 ± 0.572
0.0LysXaa: 0.0 ± 0.0
Leu
3.841LeuAla: 3.841 ± 0.494
0.369LeuCys: 0.369 ± 0.196
5.244LeuAsp: 5.244 ± 0.571
5.835LeuGlu: 5.835 ± 0.838
3.324LeuPhe: 3.324 ± 0.49
3.471LeuGly: 3.471 ± 0.62
0.96LeuHis: 0.96 ± 0.309
5.466LeuIle: 5.466 ± 0.549
8.199LeuLys: 8.199 ± 0.754
6.278LeuLeu: 6.278 ± 0.637
1.034LeuMet: 1.034 ± 0.358
5.318LeuAsn: 5.318 ± 0.545
1.92LeuPro: 1.92 ± 0.299
3.028LeuGln: 3.028 ± 0.384
2.659LeuArg: 2.659 ± 0.383
5.392LeuSer: 5.392 ± 0.563
4.579LeuThr: 4.579 ± 0.641
3.988LeuVal: 3.988 ± 0.468
0.443LeuTrp: 0.443 ± 0.219
3.619LeuTyr: 3.619 ± 0.553
0.0LeuXaa: 0.0 ± 0.0
Met
1.847MetAla: 1.847 ± 0.53
0.148MetCys: 0.148 ± 0.105
1.256MetAsp: 1.256 ± 0.251
1.182MetGlu: 1.182 ± 0.335
1.034MetPhe: 1.034 ± 0.243
0.96MetGly: 0.96 ± 0.252
0.295MetHis: 0.295 ± 0.134
1.699MetIle: 1.699 ± 0.309
2.364MetLys: 2.364 ± 0.541
2.068MetLeu: 2.068 ± 0.344
0.812MetMet: 0.812 ± 0.269
1.625MetAsn: 1.625 ± 0.318
1.108MetPro: 1.108 ± 0.254
1.847MetGln: 1.847 ± 0.467
1.182MetArg: 1.182 ± 0.291
2.068MetSer: 2.068 ± 0.475
2.437MetThr: 2.437 ± 0.419
0.812MetVal: 0.812 ± 0.231
0.517MetTrp: 0.517 ± 0.181
1.182MetTyr: 1.182 ± 0.258
0.0MetXaa: 0.0 ± 0.0
Asn
3.988AsnAla: 3.988 ± 0.668
0.295AsnCys: 0.295 ± 0.185
4.727AsnAsp: 4.727 ± 0.634
4.21AsnGlu: 4.21 ± 0.513
2.216AsnPhe: 2.216 ± 0.457
4.727AsnGly: 4.727 ± 0.53
0.886AsnHis: 0.886 ± 0.267
4.653AsnIle: 4.653 ± 0.522
6.795AsnLys: 6.795 ± 1.024
4.21AsnLeu: 4.21 ± 0.55
1.477AsnMet: 1.477 ± 0.276
5.392AsnAsn: 5.392 ± 0.669
3.176AsnPro: 3.176 ± 0.387
2.437AsnGln: 2.437 ± 0.396
2.437AsnArg: 2.437 ± 0.426
3.028AsnSer: 3.028 ± 0.385
3.324AsnThr: 3.324 ± 0.472
4.136AsnVal: 4.136 ± 0.607
0.665AsnTrp: 0.665 ± 0.17
2.807AsnTyr: 2.807 ± 0.484
0.0AsnXaa: 0.0 ± 0.0
Pro
1.182ProAla: 1.182 ± 0.281
0.148ProCys: 0.148 ± 0.113
1.477ProAsp: 1.477 ± 0.354
1.847ProGlu: 1.847 ± 0.383
1.403ProPhe: 1.403 ± 0.323
1.699ProGly: 1.699 ± 0.443
0.369ProHis: 0.369 ± 0.158
2.29ProIle: 2.29 ± 0.381
2.881ProLys: 2.881 ± 0.425
1.477ProLeu: 1.477 ± 0.255
0.96ProMet: 0.96 ± 0.256
2.437ProAsn: 2.437 ± 0.388
0.665ProPro: 0.665 ± 0.202
1.182ProGln: 1.182 ± 0.338
0.739ProArg: 0.739 ± 0.241
1.625ProSer: 1.625 ± 0.369
2.511ProThr: 2.511 ± 0.444
1.256ProVal: 1.256 ± 0.247
0.295ProTrp: 0.295 ± 0.178
1.625ProTyr: 1.625 ± 0.427
0.0ProXaa: 0.0 ± 0.0
Gln
3.324GlnAla: 3.324 ± 0.454
0.443GlnCys: 0.443 ± 0.153
1.773GlnAsp: 1.773 ± 0.356
2.733GlnGlu: 2.733 ± 0.562
2.142GlnPhe: 2.142 ± 0.434
2.585GlnGly: 2.585 ± 0.351
0.96GlnHis: 0.96 ± 0.218
3.028GlnIle: 3.028 ± 0.355
2.807GlnLys: 2.807 ± 0.527
2.807GlnLeu: 2.807 ± 0.365
0.96GlnMet: 0.96 ± 0.271
2.659GlnAsn: 2.659 ± 0.417
0.739GlnPro: 0.739 ± 0.233
1.994GlnGln: 1.994 ± 0.515
1.551GlnArg: 1.551 ± 0.405
2.142GlnSer: 2.142 ± 0.368
2.142GlnThr: 2.142 ± 0.39
2.954GlnVal: 2.954 ± 0.527
0.369GlnTrp: 0.369 ± 0.172
1.773GlnTyr: 1.773 ± 0.346
0.0GlnXaa: 0.0 ± 0.0
Arg
1.92ArgAla: 1.92 ± 0.362
0.369ArgCys: 0.369 ± 0.165
2.511ArgAsp: 2.511 ± 0.428
3.398ArgGlu: 3.398 ± 0.45
1.92ArgPhe: 1.92 ± 0.447
2.216ArgGly: 2.216 ± 0.402
1.329ArgHis: 1.329 ± 0.26
3.767ArgIle: 3.767 ± 0.533
3.545ArgLys: 3.545 ± 0.493
3.693ArgLeu: 3.693 ± 0.473
1.108ArgMet: 1.108 ± 0.283
3.028ArgAsn: 3.028 ± 0.502
1.034ArgPro: 1.034 ± 0.286
1.551ArgGln: 1.551 ± 0.336
1.329ArgArg: 1.329 ± 0.344
1.477ArgSer: 1.477 ± 0.356
1.256ArgThr: 1.256 ± 0.337
2.142ArgVal: 2.142 ± 0.323
0.739ArgTrp: 0.739 ± 0.197
2.068ArgTyr: 2.068 ± 0.4
0.0ArgXaa: 0.0 ± 0.0
Ser
4.358SerAla: 4.358 ± 0.491
0.074SerCys: 0.074 ± 0.074
4.358SerAsp: 4.358 ± 0.544
3.471SerGlu: 3.471 ± 0.452
2.659SerPhe: 2.659 ± 0.445
3.915SerGly: 3.915 ± 0.617
0.591SerHis: 0.591 ± 0.205
5.244SerIle: 5.244 ± 0.734
5.023SerLys: 5.023 ± 0.607
3.693SerLeu: 3.693 ± 0.514
1.847SerMet: 1.847 ± 0.416
3.398SerAsn: 3.398 ± 0.535
1.329SerPro: 1.329 ± 0.381
2.437SerGln: 2.437 ± 0.429
2.437SerArg: 2.437 ± 0.406
3.841SerSer: 3.841 ± 0.452
3.693SerThr: 3.693 ± 0.397
3.545SerVal: 3.545 ± 0.481
0.812SerTrp: 0.812 ± 0.192
1.477SerTyr: 1.477 ± 0.31
0.0SerXaa: 0.0 ± 0.0
Thr
3.176ThrAla: 3.176 ± 0.459
0.148ThrCys: 0.148 ± 0.099
3.988ThrAsp: 3.988 ± 0.465
3.471ThrGlu: 3.471 ± 0.469
2.954ThrPhe: 2.954 ± 0.516
3.915ThrGly: 3.915 ± 0.475
1.329ThrHis: 1.329 ± 0.338
5.17ThrIle: 5.17 ± 0.717
5.318ThrLys: 5.318 ± 0.624
4.506ThrLeu: 4.506 ± 0.486
1.108ThrMet: 1.108 ± 0.304
4.21ThrAsn: 4.21 ± 0.579
1.551ThrPro: 1.551 ± 0.349
2.29ThrGln: 2.29 ± 0.45
2.585ThrArg: 2.585 ± 0.423
4.284ThrSer: 4.284 ± 0.738
4.136ThrThr: 4.136 ± 0.466
3.471ThrVal: 3.471 ± 0.497
0.665ThrTrp: 0.665 ± 0.246
2.511ThrTyr: 2.511 ± 0.412
0.0ThrXaa: 0.0 ± 0.0
Val
3.767ValAla: 3.767 ± 0.639
0.295ValCys: 0.295 ± 0.131
4.579ValAsp: 4.579 ± 0.652
6.204ValGlu: 6.204 ± 0.62
1.699ValPhe: 1.699 ± 0.361
2.954ValGly: 2.954 ± 0.485
0.739ValHis: 0.739 ± 0.22
4.727ValIle: 4.727 ± 0.518
6.352ValLys: 6.352 ± 0.632
4.21ValLeu: 4.21 ± 0.579
1.699ValMet: 1.699 ± 0.306
2.807ValAsn: 2.807 ± 0.439
2.954ValPro: 2.954 ± 0.501
1.329ValGln: 1.329 ± 0.29
2.29ValArg: 2.29 ± 0.375
3.545ValSer: 3.545 ± 0.635
4.284ValThr: 4.284 ± 0.509
3.988ValVal: 3.988 ± 0.516
0.591ValTrp: 0.591 ± 0.197
2.216ValTyr: 2.216 ± 0.511
0.0ValXaa: 0.0 ± 0.0
Trp
0.739TrpAla: 0.739 ± 0.231
0.074TrpCys: 0.074 ± 0.06
0.517TrpAsp: 0.517 ± 0.234
0.886TrpGlu: 0.886 ± 0.209
0.665TrpPhe: 0.665 ± 0.169
0.739TrpGly: 0.739 ± 0.233
0.222TrpHis: 0.222 ± 0.113
0.739TrpIle: 0.739 ± 0.269
0.886TrpLys: 0.886 ± 0.219
1.182TrpLeu: 1.182 ± 0.264
0.295TrpMet: 0.295 ± 0.143
0.812TrpAsn: 0.812 ± 0.225
0.074TrpPro: 0.074 ± 0.057
0.665TrpGln: 0.665 ± 0.207
0.443TrpArg: 0.443 ± 0.202
0.96TrpSer: 0.96 ± 0.348
0.96TrpThr: 0.96 ± 0.258
0.812TrpVal: 0.812 ± 0.21
0.0TrpTrp: 0.0 ± 0.0
0.443TrpTyr: 0.443 ± 0.154
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.92TyrAla: 1.92 ± 0.387
0.222TyrCys: 0.222 ± 0.133
2.585TyrAsp: 2.585 ± 0.57
3.693TyrGlu: 3.693 ± 0.563
1.403TyrPhe: 1.403 ± 0.338
2.807TyrGly: 2.807 ± 0.571
0.812TyrHis: 0.812 ± 0.232
3.25TyrIle: 3.25 ± 0.522
4.062TyrLys: 4.062 ± 0.572
3.324TyrLeu: 3.324 ± 0.452
0.886TyrMet: 0.886 ± 0.268
3.102TyrAsn: 3.102 ± 0.505
1.403TyrPro: 1.403 ± 0.357
1.699TyrGln: 1.699 ± 0.29
2.29TyrArg: 2.29 ± 0.408
2.364TyrSer: 2.364 ± 0.446
2.585TyrThr: 2.585 ± 0.39
2.585TyrVal: 2.585 ± 0.383
0.812TyrTrp: 0.812 ± 0.218
1.551TyrTyr: 1.551 ± 0.409
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 74 proteins (13540 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski